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重组人Tum-5融合蛋白的克隆、表达、纯化及鉴定 被引量:1
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作者 马楠 张英起 颜真 《第四军医大学学报》 北大核心 2005年第14期1279-1281,共3页
目的:构建人肿瘤抑素(tumstatin)活性片段Tum5的融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白.方法:人工合成Tum5基因,进行测序分析,将该基因克隆入原核表达载体pQE30中,转化感受态细胞DH5α,经IPTG诱导表达重... 目的:构建人肿瘤抑素(tumstatin)活性片段Tum5的融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白.方法:人工合成Tum5基因,进行测序分析,将该基因克隆入原核表达载体pQE30中,转化感受态细胞DH5α,经IPTG诱导表达重组融合蛋白,对表达产物进行TricineSDSPAGE电泳分析和WesternBlot检测分析.结果构建了重组融合表达质粒pQE30Tum5,表达的蛋白经TricineSDSPAGE电泳分析,在约Mr10×103处出现了一条新生的蛋白条带,经灰度扫描检测,表达量约占菌体总蛋白的40%,纯化后的蛋白灰度扫描检测,纯度为95%.结论:成功地构建了人Tum5基因的原核表达载体,并纯化了该基因的原核表达产物. 展开更多
关键词 tumSTATIN tum-5 重组融合蛋白质类/分离和提纯 pQE载体
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Energy,momentum and angular momentum in the dyadosphere of a charged spacetime in teleparallel equivalent of general relativity
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作者 Gamal G.L.Nashed 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第3期85-91,共7页
We apply the energy momentum and angular momentum tensor to a tetrad field, with two unknown functions of radial coordinate, in the framework of a teleparallel equivalent of general relativity (TEGR). The definition... We apply the energy momentum and angular momentum tensor to a tetrad field, with two unknown functions of radial coordinate, in the framework of a teleparallel equivalent of general relativity (TEGR). The definition of the gravitational energy is used to investigate the energy within the external event horizon of the dyadosphere region for the Reissner-NordstrSm black hole. We also calculate the spatial momentum and angular momentum. 展开更多
关键词 Teleparallel equivalent of general relativity energy-momentum tensor angular momen- tum tensor angular momentum
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携带Tum5基因的慢病毒表达载体的构建及其在人脐静脉血管内皮细胞中的表达 被引量:2
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作者 王爱媛 高殿文 +3 位作者 王桂玲 陈立中 盖春柳 陈晓隆 《国际眼科杂志》 CAS 2008年第1期53-55,共3页
目的:构建及鉴定Tum5基因慢病毒表达载体,并观察在体外人血管内皮细胞中的表达。方法:采用PCR技术从含有tumstatin基因的质粒克隆模板pSPORT1-Sfi钓取Tum5基因,并将基因克隆到慢病毒载体表达质粒pGC-FU(含EGFP基因)中,构建慢病毒载体表... 目的:构建及鉴定Tum5基因慢病毒表达载体,并观察在体外人血管内皮细胞中的表达。方法:采用PCR技术从含有tumstatin基因的质粒克隆模板pSPORT1-Sfi钓取Tum5基因,并将基因克隆到慢病毒载体表达质粒pGC-FU(含EGFP基因)中,构建慢病毒载体表达质粒pGC-FU-Tum5,通过酶切、测序验证Tum5基因后,将pGC-FU-Tum5质粒和包装质粒pHelper1.0,pHelp-er2.0共同转染人胚胎肾上皮细胞系293T细胞,获得携带Tum5基因和EGFP基因的重组慢病毒GC-FU-Tum5,并转染靶细胞人脐静脉血管内皮细胞。通过检测标志蛋白-增强型绿色荧光蛋白(EGFP)和目的蛋白Tum5进一步验证pGC-FU-Tum5在靶细胞中Tum5的表达。结果:(1)pGC-FU-Tum5中携有正确的Tum5基因,并能在人类细胞中表达;pGC-FU-Tum5共转染包装细胞293T能产生重组病毒GC-FU-Tum5;(2)目的基因Tum5能被重组慢病毒高效地转导入靶细胞人脐静脉血管内皮细胞,并达到稳定的表达,荧光显微镜下能直接观察到GFP,Western blotting能检测到Tum5蛋白在靶细胞中的表达。结论:成功构建了携带Tum5基因的重组慢病毒载体,转染人脐静脉血管内皮细胞后能够稳定表达Tum5基因,为进一步研究Tum5的功能和眼部新生血管疾病的治疗奠定基础。 展开更多
关键词 tum5 慢病毒载体 基因转移
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逐饮Ⅰ号对恶性胸水大鼠模型血清TUM2-PK含量和胸膜组织bFGF表达的影响 被引量:1
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作者 刘建秋 李竹英 蒋鹏娜 《中华中医药学刊》 CAS 2009年第2期234-236,共3页
目的:观察逐饮Ⅰ号对恶性胸水大鼠模型血清中肿瘤M2型丙酮酸激酶(TUM2-PK)和胸膜组织中碱性成纤维细胞因子(bFGF)的影响。方法:建立艾氏腹水瘤细胞株(EAC)恶性胸水大鼠模型,用ELISA法检测恶性胸水大鼠血清中TUM2-PK的含量,免疫组化法测... 目的:观察逐饮Ⅰ号对恶性胸水大鼠模型血清中肿瘤M2型丙酮酸激酶(TUM2-PK)和胸膜组织中碱性成纤维细胞因子(bFGF)的影响。方法:建立艾氏腹水瘤细胞株(EAC)恶性胸水大鼠模型,用ELISA法检测恶性胸水大鼠血清中TUM2-PK的含量,免疫组化法测定胸膜组织中bFGF的表达。结果:①恶性胸水大鼠模型组的血清TUM2-PK含量明显高于正常空白组,中药组、顺铂组、中药+顺铂组血清TUM2-PK含量明显低于模型组,中药+顺铂组的血清TUM2-PK含量明显低于顺铂组和中药组,中药组与顺铂组含量相近。②恶性胸水大鼠模型组的胸膜组织bFGF表达,明显高于空白组,中药组、顺铂组、中药加顺铂组的bFGF表达低于模型组,中药+顺铂组的bFGF表达,明显低于顺铂组和中药组,中药组与顺铂组相近。结论:①逐饮Ⅰ号可明显降低恶性胸水大鼠模型血清TUM2-PK含量。②逐饮Ⅰ号可明显降低恶性胸水大鼠模型胸膜组织bFGF表达。③逐饮Ⅰ号可能通过降低恶性胸水大鼠模型血清TUM2-PK含量及胸膜组织bFGF表达,达到抑制恶性胸水的目的。 展开更多
关键词 逐饮Ⅰ号 恶性胸水大鼠模型 tum2-PK BFGF
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乌尔第三王朝历史文献中.TUM词义辨析 被引量:1
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作者 吴宇虹 董晓博 《史学史研究》 CSSCI 北大核心 2016年第2期120-123,共4页
E.TUM 是大量出现在乌尔第三王朝档案管理类文件(Bulla&Archive Label)中的行政术语,其所属语种及所表意义学术界素有争论。本文拟通过对相关研究进行梳理,结合已公开发表的相关文献,并做简要分析,以期为该问题的解决做出些许贡献。
关键词 乌尔第三王朝 tum 档案箱 历史文献 阿卡德语 档案管理 第三时期 达干 亨佩尔 舒勒
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一种新的肿瘤标志物──恶性肿瘤特异性生长因子(TUM)的测定及临床应用 被引量:4
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作者 王术艺 艾桂萍 《陕西医学检验》 1997年第2期40-42,共3页
通过对62例恶性肿瘤患者和40例良性疾病患者及36例健康人测定血清恶性肿瘤特异性生长因子(TUM)的浓度,发现恶性肿瘤血清TUM的水平明显高于良性疾病组和健康对照组(P<0.01).值得重视的是肺癌阳性率可达81.2%.另外对32例恶性... 通过对62例恶性肿瘤患者和40例良性疾病患者及36例健康人测定血清恶性肿瘤特异性生长因子(TUM)的浓度,发现恶性肿瘤血清TUM的水平明显高于良性疾病组和健康对照组(P<0.01).值得重视的是肺癌阳性率可达81.2%.另外对32例恶性肿瘤患者同时检测TUM与CEA,TUM的阳性率为65.6%,CEA阳性率为43.8%,两种检测联和阳性率为81.3%.因此TUM作为新的肿瘤标志物有助于恶性肿瘤的早期筛选诊断. 展开更多
关键词 血清 tum 肿瘤标物志物 测定
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Laparoscopic endoscopic cooperative surgery as a minimally invasive treatment for gastric submucosal tumor 被引量:7
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作者 Tsutomu Namikawa Kazuhiro Hanazaki 《World Journal of Gastrointestinal Endoscopy》 CAS 2015年第14期1150-1156,共7页
Laparoscopic wedge resection is a useful procedure fortreating patients with submucosal tumor(SMT) including gastrointestinal stromal tumor(GIST) of the stomach. However, resection of intragastric-type SMTs can be pro... Laparoscopic wedge resection is a useful procedure fortreating patients with submucosal tumor(SMT) including gastrointestinal stromal tumor(GIST) of the stomach. However, resection of intragastric-type SMTs can be problematic due to the difficulty in accurately judging the location of endoluminal tumor growth, and often excessive amounts of healthy mucosa are removed; thus, full-thickness local excision using laparoscopic and endoscopic cooperative surgery(LECS) is a promising procedure for these cases. Our experience with LECS has confirmed this procedure to be a safe, feasible, and minimally invasive treatment method for gastric GISTs less than 5 cm in diameter, with outcomes similar to conventional laparoscopic wedge resection. The important advantage of LECS is the reduction in the resected area of the gastric wall compared to that in conventional laparoscopic wedge resection using a linear stapler. Early gastric cancer fits the criteria for endoscopic resection; however, if performing endoscopic submucosal dissection is difficult, the LECS procedure might be a good alternative. In the future, LECS is also likely to be indicated for duodenal tumors, as well as gastric tumors. Furthermore, developments in endoscopic and laparoscopic technology have generated various modified LECS techniques, leading to even less invasive surgery. 展开更多
关键词 COOPERATIVE surgery ENDOSCOPY GASTROINTESTINAL tum
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Tum5蛋白多克隆抗体的制备及纯化
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作者 李迪 张更 +5 位作者 武国军 刘飞 王禾 秦卫军 袁建林 于磊 《中国现代医学杂志》 CAS CSCD 北大核心 2014年第11期9-13,共5页
目的制备Tum5蛋白多克隆抗体并纯化。方法利用pQE30-Tum5重组质粒在大肠杆菌中通过IPTG诱导表达;亲和层析法纯化重组蛋白;以纯化的重组蛋白为抗原免疫新西兰大耳白兔制备抗Tum5多克隆抗体,ELISA方法检测抗体滴度,亲和层析法纯化兔抗Tum... 目的制备Tum5蛋白多克隆抗体并纯化。方法利用pQE30-Tum5重组质粒在大肠杆菌中通过IPTG诱导表达;亲和层析法纯化重组蛋白;以纯化的重组蛋白为抗原免疫新西兰大耳白兔制备抗Tum5多克隆抗体,ELISA方法检测抗体滴度,亲和层析法纯化兔抗Tum5多克隆抗体。结果利用pQE30-Tum5重组质粒,经原核表达和亲和层析获得Tum5蛋白;利用Tum5蛋白制备多克隆抗体,纯度大于95%。结论利用原核表达的Tum5蛋白成功地制备了兔抗Tum5多克隆抗体。 展开更多
关键词 tum5 亲和层析纯化 多克隆抗体
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血清TuM2-PK、TSGF与CEA、CA19-9、CA242联合检测在结肠癌诊断中的临床价值 被引量:20
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作者 荆卫娟 任传路 +2 位作者 丁磊 王海刚 丁庆莉 《标记免疫分析与临床》 CAS 2015年第11期1121-1124,共4页
目的探讨血清Tu M2-PK、TSGF与CEA、CA19-9、CA242联合检测在结肠癌诊断中的临床应用价值。方法采集83例结肠癌患者和90例健康体检者血清,运用ELISA法检测Tu M2-PK、速率法检测TSGF、化学发光法检测CEA、CA19-9和CA242水平。结果与对照... 目的探讨血清Tu M2-PK、TSGF与CEA、CA19-9、CA242联合检测在结肠癌诊断中的临床应用价值。方法采集83例结肠癌患者和90例健康体检者血清,运用ELISA法检测Tu M2-PK、速率法检测TSGF、化学发光法检测CEA、CA19-9和CA242水平。结果与对照组相比,结肠癌组血清Tu M2-PK、TSGF、CEA、CA19-9、CA242水平均升高,差异有统计学意义(均P<0.05)。TSGF、Tu M2-PK诊断结肠癌的敏感度高于CA242、CA19-9单独检测,差异具统计学意义(均P<0.05);上述几种标志物单独检测在结肠癌诊断中敏感度均不高,最高仅为65.06%。CA242、CA19-9诊断特异度均高于TSGF、CEA单独检测,差异具统计学意义(均P<0.05)。诊断准确度指标间差异无统计学意义(P>0.05)。组合CA242+CA19-9+TSGF与组合CA242+CA19-9相比,诊断敏感度提高具统计学意义(P<0.05),但特异度降低明显(P<0.05),低达66.67%。组合CA242+CA19-9+TSGF+Tu M2-PK较组合CA242+CA19-9,敏感度得到更大提高(P<0.05),高达72.29%;虽特异度仍有降低,但降低不具统计学意义(P>0.05);同时这一组合使结肠癌诊断的准确度得到了提高,高达75.72%,但差异无统计学意义(P>0.05)。组合CA242+CA19-9+TSGF+Tu M2-PK在加或不加CEA这一指标情况下其诊断效率指标均无变化。结论联合检测组合CA242+CA19-9+TSGF+Tu M2-PK能有效提高单项检测对结肠癌的诊断效率,尤其提高特异度很高的CA242、CA19-9诊断结肠癌的敏感度和准确度。将这几项指标进行联合检测分析可能会更有助于我们对结肠癌的筛查和诊断。 展开更多
关键词 肿瘤型M2丙酮酸激酶(tum2-PK) TSGF CEA CA19-9 CA242 联合检测 结肠癌
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PcDNA3.1-Tum-5基因真核表达载体的构建
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作者 张军 兰斌 孙启强 《肿瘤基础与临床》 2010年第6期461-463,共3页
目的构建携带Tum-5基因真核表达载体PcDNA3.1-Tum-5。方法利用巢式PCR扩增人胚肾细胞株HEK293 cDNA获得Tum-5基因,并利用DNA回收试剂盒进行回收;同时扩增、提取pcDNA3.1质粒,并将其与Tum-5基因分别进行EcoRV和XhoI双酶切,回收相应条带,... 目的构建携带Tum-5基因真核表达载体PcDNA3.1-Tum-5。方法利用巢式PCR扩增人胚肾细胞株HEK293 cDNA获得Tum-5基因,并利用DNA回收试剂盒进行回收;同时扩增、提取pcDNA3.1质粒,并将其与Tum-5基因分别进行EcoRV和XhoI双酶切,回收相应条带,通过T4DNA连接酶进行连接,转化DH5α感受态细胞,获得阳性克隆。获得质粒DNA后行KpnI和XhoI双酶切,克隆并测序酶切产物。结果巢式PCR扩增出的产物与目的基因大小相同;重组质粒经双酶切电泳分析,与预期结果一致;对连接点两端进行测序,结果显示接头两端序列连接正确,插入序列与载体读码框架相匹配。结论本方法可以成功构建人Tum-5基因表达载体,为进一步研究Tum-5基因功能提供了理论依据。 展开更多
关键词 肿瘤抑素 质粒 tum-5基因 巢式PCR
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TuM2-PK检测在消化道肿瘤中的应用价值
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作者 刘发蓉 马颖才 +1 位作者 费发珠 白菊芳 《临床医学进展》 2022年第7期6090-6096,共7页
食管、胃、结直肠等部位的肿瘤总称为消化道肿瘤,由于早期消化道肿瘤患者没有明显的临床症状,从而导致就诊时基本都是肿瘤的中晚期,因此治疗效果差,预后欠佳。大量的研究和临床数据证实,预防和降低死亡率最有效的办法就是早诊断、早治... 食管、胃、结直肠等部位的肿瘤总称为消化道肿瘤,由于早期消化道肿瘤患者没有明显的临床症状,从而导致就诊时基本都是肿瘤的中晚期,因此治疗效果差,预后欠佳。大量的研究和临床数据证实,预防和降低死亡率最有效的办法就是早诊断、早治疗。肿瘤的发生、发展与肿瘤标记物的表达水平在一定程度上显示相关性,因此,寻找可用于早期诊断和预后判断的肿瘤标志物一直是人们关注的焦点。M2型丙酮酸激酶(pyruvate kinase isoenzyme type M2, PKM2)是糖酵解过程中的限速酶丙酮酸激酶的同分异构体中的一种,可通过瓦伯格效果促进肿瘤细胞的生长与增值,是肿瘤能量代谢的重要标志,是一种潜在的新型肿瘤标记物,作者对其进行了综述和梳理。 展开更多
关键词 tum2-PK 消化道肿瘤 综述
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STUDY ON STRUCTURE AND PROPERTY OF Nb-DOPED TiC CERAMIC WITH QUANTUM CHEMISTRY CALCULATIONS
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作者 闵新民 蔡克峰 +1 位作者 南策文 袁润章 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 1994年第4期63-71,共9页
Nb-doped TiC ceramic,or (NbyTi1-y) Cx,in which amount of Nb element added is increased from zero to 40Wt. %, synthisized -with self-propagating high temperature synthesis,is studied with SCF-Xa-DV,a quantum chemistry ... Nb-doped TiC ceramic,or (NbyTi1-y) Cx,in which amount of Nb element added is increased from zero to 40Wt. %, synthisized -with self-propagating high temperature synthesis,is studied with SCF-Xa-DV,a quantum chemistry cal-culating method. The chemical bonding is studied to discuss the relation between structrues and properties. Several classes of models in which there is no vacancy,one vacancy or two vacan-cies have been calculated. From the calculated results of bond or-der, a measure of covalent bond strength,and molecule orbital contour map, it is concluded that when Nb element added in-creases, the vacancies increase correspondingly,the covalent com-ponent of chemical bonds of the samples decreases -while the met-al-bonding component increases, so the hardness and resistance of the samples decrease. 展开更多
关键词 TiC ceramic structure and property quan- tum chemistry
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Effect of tumor necrosis factor-α antagonists on oxidative stress in patients with Crohn's disease
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作者 Kazunari Yamamoto Toshimi Chiba Takayuki Matsumoto 《World Journal of Gastroenterology》 SCIE CAS 2015年第35期10208-10214,共7页
AIM: To investigate changes in oxidative stress in Crohn's disease(CD) before and after anti-tumor necrosis factor(TNF)-α treatment.METHODS: A total of 42 patients with active CD, who were scheduled to be treated... AIM: To investigate changes in oxidative stress in Crohn's disease(CD) before and after anti-tumor necrosis factor(TNF)-α treatment.METHODS: A total of 42 patients with active CD, who were scheduled to be treated by anti-TNF-α antibodies, were enrolled. Serum levels of diacron-reactive oxygen metabolites(d-ROM), biological antioxidant potential(BAP), and modified ratio of oxidative stress and antioxidant capacity(m-OA) were measured using the Free Radical Analytical System before and 8 wk after induction of therapy with infliximab or adalimumab. The values for oxidative stress were correlated with disease activity and clinical response as determined by the CD activity index(CDAI) at 8 and 54 wk after the therapy.RESULTS: Prior to treatment, d-ROM showed significant correlations with CDAI(r = 0.42, P < 0.01). There was a significant negative correlation between m-OA and CDAI before and after treatment(r =-0.48 vs r =-0.42, P < 0.01). CDAI and d-ROM had decreased significantly by 8 wk after treatment(CDAI; 223.3 ± 113.2 vs 158.3 ± 73.4, P < 0.01, d-ROM; 373 ± 133 vs 312 ± 101, P < 0.05). However, neither BAP nor m-OA had changed significantly. In patients who had responded to the treatment at 8 wk, d-ROM, BAP, and m-OA levels before treatment did not differ significantly between patients with and without loss of response.CONCLUSION: Anti-TNF-α therapy decreases oxi-dative stress in patients with CD, but does not alter the production of antioxidants. Dysregulation of antioxidants may be associated with the disease. 展开更多
关键词 Crohn’s DISEASE SEVERITY OXIDATIVE stress Anti-tum
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血清ESM-1、TuM2-PK水平与直肠癌患者病情严重程度和预后的关系
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作者 王涛 段占峰 张巍巍 《实用癌症杂志》 2024年第12期1976-1979,共4页
目的探讨直肠癌患者血清ESM-1、TuM2-PK水平及临床意义。方法选取直肠癌患者120例,入院时TNM分期,Ⅰ~Ⅱ期18例、Ⅲ期58例、Ⅳ期44例,根据实体瘤治疗疗效评价标准评估预后分为预后良好组30例,预后不良组90例。比较不同临床分期血清ESM-1... 目的探讨直肠癌患者血清ESM-1、TuM2-PK水平及临床意义。方法选取直肠癌患者120例,入院时TNM分期,Ⅰ~Ⅱ期18例、Ⅲ期58例、Ⅳ期44例,根据实体瘤治疗疗效评价标准评估预后分为预后良好组30例,预后不良组90例。比较不同临床分期血清ESM-1、TuM2-PK水平,并分析其与临床分期的相关性,比较不同预后患者入院时、化疗1个周期后、化疗2个周期后血清ESM-1、TuM2-PK水平,并分析联合指标检测对直肠癌患者预后的判断价值。结果不同分期入院时患者血清ESM-1、TuM2-PK水平比较,P<0.05;入院时血清ESM-1、TuM2-PK水平与TNM分期成正比(γ=0.782,P<0.001;γ=0.320,P<0.001);不同预后患者入院时血清ESM-1、TuM2-PK水平比较,P>0.05,化疗1、2个周期后,预后不良组患者血清ESM-1、TuM2-PK水平均高于预后良好组,P<0.05;化疗1、2个周期后,血清ESM-1、TuM2-PK水平联合预测AUC为0.926、0.991,敏感度为94.4%、98.9%,特异度为83.3%、93.3%。结论直肠癌患者血清ESM-1、TuM2-PK水平与临床分期和预后有一定相关性,并且联合检测可以有效预测患者预后。 展开更多
关键词 直肠癌 ESM-1 tum2-PK TNM分期 预后
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Antibacterial activity of Leptogium cochleatum and Leptogium moluccanum
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作者 Andrea Pauline B.Manlapaz Mitzi I.Mariano +2 位作者 Odessa Rona M.Reyes Laureena C.Rodriguez Jaycee Augusto G.Paguirigan 《Studies in Fungi》 2022年第1期139-142,共4页
Currently there is an increase in the emergence and re-emergence of bacterial infections,and lichens are potential sources of pharmaceutically important secondary metabolites to halt this problem.Lichens are unique pl... Currently there is an increase in the emergence and re-emergence of bacterial infections,and lichens are potential sources of pharmaceutically important secondary metabolites to halt this problem.Lichens are unique plant-like organisms which are relatively less explored in the Philippines.Here,lichen extracts of Leptogium cochleatum and Leptogium moluccanum were tested against Escherichia coli,Enterobacter agglomerans,Klebsiella pneumoniae,Pseudomonas aeruginosa,and Staphylococcus aureus using paper disk diffusion assay.Hexane extract of L.moluccanum led to a 17.14 mm zone of inhibition(ZOI)against K.pneumoniae,while hexane extract of L.cochleatum led to a 15.28 mm ZOI against E.agglomerans.Dichloromethane extracts of L.cochleatum exhibited a ZOI of 15.86 mm against E.agglomerans and 14.84 mm ZOI against E.coli.The bioactivities exhibited by the lichen extracts may be due to the secondary metabolites detected using thin layer chromatography.Indeed,lichen-derived bioactive compounds hold great promise for pharmaceutical applications for the benefit of human life. 展开更多
关键词 LEP tum BENEFIT
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Theoretical study of photon emission from single quantum dot emitter coupled to surface plasmons
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作者 Guang-cun SHAN Shu-ying BAO +1 位作者 Kang ZHANG Wei HUANG 《Frontiers of physics》 SCIE CSCD 2011年第3期313-319,共7页
Motivated by the recent pioneering advances on nanoscale plasmonics and also nanophotonics tech- nology based on the surface plasmons (SPs), in this work, we give a master equation model in the Lindblad form and inv... Motivated by the recent pioneering advances on nanoscale plasmonics and also nanophotonics tech- nology based on the surface plasmons (SPs), in this work, we give a master equation model in the Lindblad form and investigate the quantum optical properties of single quantum dot (QD) emitter coupled to the SPs of a metallic nanowire. Our main results demonstrate the QD luminescence results of photon emission show three distinctive regimes depending on the distance between QD and metallic nanowire, which elucidates a crossover passing from being metallic dissipative for much smaller emitter-nanowire distances to surface plasmon (SP) emission for larger separations at the vicinity of plasmonic metallic nanowire. Besides, our results also indicate that, for both the resonant case and the detuning case, through measuring QD emitter luminescence spectra and second-order correlation functions, the information about the QD emitter coupling to the SPs of the dissipative metallic nanowire can be extracted. This theoretical study will serve as an introduction to un- derstanding the nanoplasmonic imaging spectroscopy and pave a new way to realize the quantum information devices. 展开更多
关键词 quantum plasmonics quantum optics metallic nanowire surface plasmon (SP) quan-tum dot
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pLXSN-Tum-5致人脐静脉内皮细胞凋亡作用及机制 被引量:1
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作者 唐泽波 温娜 +2 位作者 齐玲 历春 盖晓东 《中国公共卫生》 CAS CSCD 北大核心 2017年第9期1360-1363,共4页
目的探讨pLXSN-Tum-5病毒颗粒对人脐静脉内皮细胞(HUVEC)的生长抑制和诱导凋亡作用及机制。方法采用不同浓度的pLXSN-Tum-5病毒颗粒作用HUVEC,利用噻唑蓝法和Hoechst-33342染色法检测细胞生长及其形态变化;RT-PCR和Western blotting方... 目的探讨pLXSN-Tum-5病毒颗粒对人脐静脉内皮细胞(HUVEC)的生长抑制和诱导凋亡作用及机制。方法采用不同浓度的pLXSN-Tum-5病毒颗粒作用HUVEC,利用噻唑蓝法和Hoechst-33342染色法检测细胞生长及其形态变化;RT-PCR和Western blotting方法检测细胞中Bax、Bcl-2、caspase-3 mRNA和蛋白表达变化。结果与对照组(pLXSN)比较,pLXSN-Tum-5病毒颗粒转染组HUVEC的存活率明显降低,呈剂量效应关系;细胞内可见凋亡小体;对照组HUVEC内Bcl-2和caspase-3 mRNA和蛋白表达分别为(0.721±0.041)、(0.654±0.034)和(0.956±0.032)、(0.356±0.054);与对照组比较,20μmol/L pLXSN-Tum-5病毒颗粒转染组HUVEC内Bcl-2和caspase-3mRNA和蛋白水平[分别为(1.134±0.0524)、(1.012±0.0641)和(1.612±0.067)、(0.712±0.0647)]明显上调(P<0.05)。结论 Tum-5可抑制HUVEC增殖,诱导HUVEC凋亡,其机制可能与上调Bax/Bcl-2和caspase-3表达有关。 展开更多
关键词 肿瘤抑素(tumstatin) tum-5 细胞凋亡 人脐静脉内皮细胞(HUVEC) 抗血管生成
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Interfacial polaron in quantum dots and luminescent porous silicon 被引量:2
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作者 Bingsuo Zou Sishen Xie 《Chinese Science Bulletin》 SCIE EI CAS 2001年第8期630-635,共6页
Polaronic effect is important in the current researches on quantum dots (QD). This paper reported a new concept of the 'confined polaron', their size dependent formation possibilities and energy variation in d... Polaronic effect is important in the current researches on quantum dots (QD). This paper reported a new concept of the 'confined polaron', their size dependent formation possibilities and energy variation in different QD systems, with an indication of contribution from both intrinsic and/or extrinsic phonons. To understand the spectro-scopic characteristics of porous silicon (PS), we find that luminescence behavior of oxidized porous silicon is in good agreement with the model of interfacial confined polaron in QDs. This conclusion is useful to unveiling the mechanmism of PS luminescence. 展开更多
关键词 porous silicon LUMINESCENCE INTERFACIAL POLARON quan- tum dots.
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边缘感知增强的煤矿井下视觉SLAM方法 被引量:2
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作者 牟琦 梁鑫 +2 位作者 郭媛婕 王煜豪 李占利 《煤田地质与勘探》 北大核心 2025年第3期231-242,共12页
【目的】煤矿井下普遍存在低照度、弱纹理和结构化的特征退化场景,导致视觉SLAM(visual simultaneous localization and mapping)系统面临有效特征不足或误匹配率高的问题,严重制约了其定位的准确性和鲁棒性。【方法】提出一种基于边缘... 【目的】煤矿井下普遍存在低照度、弱纹理和结构化的特征退化场景,导致视觉SLAM(visual simultaneous localization and mapping)系统面临有效特征不足或误匹配率高的问题,严重制约了其定位的准确性和鲁棒性。【方法】提出一种基于边缘感知增强的视觉SLAM方法。首先,构建了边缘感知约束的低光图像增强模块。通过自适应尺度的梯度域引导滤波器优化Retinex算法,以获得纹理清晰光照均匀的图像,从而显著提升了在低光照和不均匀光照条件下特征提取性能。其次,在视觉里程计中构建了边缘感知增强的特征提取和匹配模块,通过点线特征融合策略有效增强了弱纹理和结构化场景中特征的可检测性和匹配准确性。具体使用边缘绘制线特征提取算法(edge drawing lines,EDLines)提取线特征,定向FAST和旋转BRIEF点特征提取算法(oriented fast and rotated brief,ORB)提取点特征,并利用基于网格运动统计(grid-based motion statistics,GMS)和比值测试匹配算法进行精确匹配。最后,将该方法与ORB-SLAM2、ORB-SLAM3在TUM数据集和煤矿井下实景数据集上进行了全面实验验证,涵盖图像增强、特征匹配和定位等多个环节。【结果和结论】结果表明:(1)在TUM数据集上的测试结果显示,所提方法与ORB-SLAM2相比,绝对轨迹误差、相对轨迹误差的均方根误差分别降低了4%~38.46%、8.62%~50%;与ORB-SLAM3相比,绝对轨迹误差、相对轨迹误差的均方根误差分别降低了0~61.68%、3.63%~47.05%。(2)在煤矿井下实景实验中,所提方法的定位轨迹更接近于相机运动参考轨迹。(3)有效提高了视觉SLAM在煤矿井下特征退化场景中的准确性和鲁棒性,为视觉SLAM技术在煤矿井下的应用提供了技术解决方案。研究面向井下特征退化场景的视觉SLAM方法,对于推动煤矿井下移动式装备机器人化具有重要意义。 展开更多
关键词 视觉SLAM 特征退化 边缘感知 图像增强 点线特征融合 tum数据集
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TUM Vice President Visits
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《Tsinghua Science and Technology》 SCIE EI CAS 2008年第5期695-695,共1页
Vice President Meng Liqiu of Technical University of Munich (TUM) visited Tsinghua University on July 7, 2008. Tsinghua Vice President Kang Kejun had a discussion with Professor Meng. VP Kang briefed Professor Meng ... Vice President Meng Liqiu of Technical University of Munich (TUM) visited Tsinghua University on July 7, 2008. Tsinghua Vice President Kang Kejun had a discussion with Professor Meng. VP Kang briefed Professor Meng on Tsinghua's recent developments in aerospace, energy, electrical engineering, life science, and management. 展开更多
关键词 tum Vice President Visits VP
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