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Numerical simulation of the preparation of semi-solid metal slurry with damper cooling tube method 被引量:1
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作者 Haoqiang Yang Shuisheng Xie Lei Li 《Journal of University of Science and Technology Beijing》 CSCD 2007年第5期443-448,共6页
In semi-solid forming process, preparing the slurry with rosette or globular microstructure is very important. A new approach named the damper cooling tube method (DCT), to produce the semi-solid metal slurry, has b... In semi-solid forming process, preparing the slurry with rosette or globular microstructure is very important. A new approach named the damper cooling tube method (DCT), to produce the semi-solid metal slurry, has been introduced. To optimize the technical parameters in designing the apparatus, the finite volume method was adopted to simulate the flow process. The temperature effects on the rheological properties of the slurries were also considered. The effects of the technical parameters on the slurry properties were studied in detail. 展开更多
关键词 semi-solid forming RHEOLOGY damper cooling tube method finite volume method
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Method for Improving Transverse Wall Thickness Precision of Seamless Steel Tube Based on Tube Rotation 被引量:3
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作者 Yong-zheng JIANG Hua-ping TANG 《Journal of Iron and Steel Research International》 SCIE EI CAS CSCD 2015年第10期924-930,共7页
The tube rotation method (TRM) refers to the rotational movement of steel tube about its axis as well as translation in rolling direction in stretch reducing rolling process. The influence of the TRM on transverse w... The tube rotation method (TRM) refers to the rotational movement of steel tube about its axis as well as translation in rolling direction in stretch reducing rolling process. The influence of the TRM on transverse wall thickness precision of seamless steel tube was studied. Thickness distribution of the TRM was obtained by superimposing the thickened amount of single pass rolling. Results show that the TRM can effectively improve the evenness of thickness distribution. In order to analyze the influence mechanism of the TRM, the finite element method was adopted to simulate the thickness distribution in stretch reduction process. Results show that the TRM changes the roundtrip flow between two fix places of conventional stretch reducing and inhibits the directional accumulation of metal. In addition, the TRM has a correction effect on thickness cusp. All these advantages of the TRM help to improve the transverse wall thickness precision of seamless steel tube. 展开更多
关键词 seamless tube stretch reducing transverse unevenness tube rotation method
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The pressure field in the liquid column in the tube-arrest method 被引量:2
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作者 应崇福 李超 +1 位作者 徐德龙 邓京军 《Chinese Physics B》 SCIE EI CAS CSCD 2008年第7期2580-2589,共10页
We have been using the method of tube-arrest as a means of producing transient single cavitation bubble. In the present paper we seek to comprehend the mechanism of production and inquire into the structure of the ab ... We have been using the method of tube-arrest as a means of producing transient single cavitation bubble. In the present paper we seek to comprehend the mechanism of production and inquire into the structure of the ab initio pressure field in the arrested liquid column. The generated pressure wave is shown by combining the theoretical analysis with the experimental observation to be a slightly varied version of water hammer. With relatively clean liquid, the magnitude of the tension peak generating the TSB is likely to reach of several millions Pa. It is also shown that the so generated cavitation bubble originating from the gas-containing bulk liquid is in ‘violent’ motion. 展开更多
关键词 tube-arrest method original pressure wave water hammer origin of violent cavitation bubble
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Fabrication of the 19-filament Fe/Cu clad MgB_2 wire via in situ powder-in-tube method
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作者 YU ZhengGuang MA YanWei WANG DongLiang GAO ZhaoShun ZHANG XianPing 《Chinese Science Bulletin》 SCIE EI CAS 2007年第19期2621-2625,共5页
Using commercial amorphous B powder (92% in purity) and Mg powder (99% in purity) as starting materials, 19-filament Fe/Cu clad MgB2 wires were fabricated by an in situ powder-in-tube method. Heat treatment was perfor... Using commercial amorphous B powder (92% in purity) and Mg powder (99% in purity) as starting materials, 19-filament Fe/Cu clad MgB2 wires were fabricated by an in situ powder-in-tube method. Heat treatment was performed at 700℃ for 1 h under an argon gas atmosphere. The influence of Mg/B ratio on the microstructure and superconducting properties of the wires was investigated. It was found that the major phases of MgB2 wires were MgB2 accompanied with relatively small amounts of MgO and Fe2B impurities. With 5% excess Mg addition, the onset TC slightly decreased. However, the transport JC at 4.2 K and 4 T reached 1.07×104 A·cm-2, increasing by a factor of 1.4 compared to the stoichiometric sample. Moreover, the Mg1.05B2 sample showed an improved field dependence of JC, suggesting that less voids and smaller grain size of the Mg1.05B2 core lead to better grain connectivity and stronger flux pinning. 展开更多
关键词 MgB2金属导线 铜铁复合物 制造工业 灯丝
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A CALCULATION METHOD FOR FULLY DEVELOPED FLOWS IN CURVED RECTANGULAR TUBES
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作者 卢志明 刘宇陆 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 1997年第4期315-320,共6页
In this paper, A method, consisted of perturbation method, Garlerkin method and finite-difference method, is designed to calculate fully developed flows in curved tubes of rectangular cross-section. It costs less comp... In this paper, A method, consisted of perturbation method, Garlerkin method and finite-difference method, is designed to calculate fully developed flows in curved tubes of rectangular cross-section. It costs less computation than that of direct solving N-S equations, and prevents from building high-order difference equations and extra dealing with the boundary conditions. Numerical results in the situation of small curvature and low Dean number is in accordance with former's numerical and experimental results in quality, and it shows the feasibility of this paper's method. 展开更多
关键词 curved tube Galerkin method Dean number
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HEAT TRANSFER STUDIES OF HIGHLY VISCOUS NON-NEWTONIAN FLUIDS IN VERTICAL TUBES BY FINITE ELEMENT METHOD
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作者 钱夕元 侯望奇 江体乾 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 1996年第1期65-74,共10页
A numerical method capable is developed for handling steady laminar flow and heat trans-fer of a highly viscous power-law fluid whose density,viscosity,specific heat and thermalconductivity,vary with temperature.The g... A numerical method capable is developed for handling steady laminar flow and heat trans-fer of a highly viscous power-law fluid whose density,viscosity,specific heat and thermalconductivity,vary with temperature.The governing equations are found to be continuity,monmentumand energy expressions.Important effects such as varying viscosity,natural convection and viscousdissipation are incorporated in the theoretical model.These equations are being attracted by employing a decoupled finite element method.Galerkin’sprinciple is used to handle the momentum and continuity equations.Consistent(SU/PG)andnon-consistent(SU)streamline upwind methods are employed for the energy equation.Comparisonof calculated results and experimental data shows good agreement.Similar results are obtained withSU and SU/PG methods.Velocity and temperature profiles which provide insights into the processare also given. 展开更多
关键词 non-Newtonian FLUIDS VERTICAL tube FINITE clement method
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Fabrication and characterization of highly ordered Ni_(0.5)Zn_(0.5)Fe_2O_4 nanowire/tube arrays by sol-gel template method 被引量:6
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作者 Mei Yu Jianhua Liu Songmei Li 《Journal of University of Science and Technology Beijing》 CSCD 2007年第5期469-472,共4页
Highly ordered nanowire/tube arrays of Ni0.5Zn0.5Fe2O4were fabricated by the sol-gel method in the pores of anodic alumina membrane (AAM). Whether nanowires or nanotubes were fabricated depends on immersion time. Th... Highly ordered nanowire/tube arrays of Ni0.5Zn0.5Fe2O4were fabricated by the sol-gel method in the pores of anodic alumina membrane (AAM). Whether nanowires or nanotubes were fabricated depends on immersion time. The immersion time was 15- 40 s for nanotubes and over 60 s for nanowires. The topography and crystalline structure of the nanowire arrays were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray diffraction (XRD). It was found that the length and diameter of the Ni0.5Zn0.5Fe2O4 nanowires are related to the thickness of the AAM and the diameter of the pores. The results indicated that the Ni0.5Zn0.5Fe2O4 nanowires are uniform and parallel to each other. 展开更多
关键词 anowire/tube arrays anodic alumina membrane (AAM) sol-gel method
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A Numerical-analytic Method for Quickly Predicting Springback of Numerical Control Bending of Thin-walled Tube 被引量:4
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作者 Mei ZHAN He YANG Liang HUANG 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2006年第5期713-720,共8页
Springback is one of important factors influencing the forming quality of numerical control (NC) bending of thin-walled tube. In this paper, a numerical-analytic method for springback angle prediction of the process... Springback is one of important factors influencing the forming quality of numerical control (NC) bending of thin-walled tube. In this paper, a numerical-analytic method for springback angle prediction of the process was put forward. The method is based on springback angle model derived using analytic method and simulation results from three-dimensional (3D) rigid-plastic finite element method (FEM). The method is validated through comparison with experimental results. The features of the method are as follows: (1) The method is high in efficiency because it combines advantages of rigid-plastic FEM and analytic method. (2) The method is satisfactory in accuracy, since the field variables used in the model is resulting from 3D rigid-plastic FEM solution, and the effects both of axial force and strain neutral axis shift have been included. (3) Research on multi-factor effects can be carried out using the method due to its advantage inheriting from rigid-plastic FEM. The method described here is also of general significance to other bending processes. 展开更多
关键词 Thin-walled tube Numerical control bending SPRINGBACK Numerical-analytic method 3D rigid-plastic FEM
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THE APPLICATION OF BOUNDARY ELEMENT METHOD IN STRESS ANALYSIS OF AUTOFRETTED TUBE WITH NOTCH
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作者 宋顺成 张玉诚 冯德振 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 1990年第2期185-190,共6页
In this paper, the boundary element method is applied to investigate the internal state of stress of autofretted tube with notch and the calculated results are important in the practical design.
关键词 THE APPLICATION OF BOUNDARY ELEMENT method IN STRESS ANALYSIS OF AUTOFRETTED tube WITH NOTCH
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Structure of Graphene, and Mechanical and Bonding Characteristics of Single Wall Carbon Nanotube by Linear Scaling Quantum Mechanical Method
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作者 Jun Cai Guiqin Li +1 位作者 Chongyu Wang Zhiyong Xie 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2010年第7期614-618,共5页
Using a linear scaling self-consistent-charge density functional tight binding (SCC-DFTB) and an ab initio Omol method, the bonding characteristics and Young's modulus of (10, 0) and (10,10) single-walled carbo... Using a linear scaling self-consistent-charge density functional tight binding (SCC-DFTB) and an ab initio Omol method, the bonding characteristics and Young's modulus of (10, 0) and (10,10) single-walled carbon nanotubes are calculated. The structure of a graphene is also calculated. It is found that the C-C and C-H bond length, their distribution characteristics on the tube, and Young^s modulus of the tube by linear scaling SCC-DFTB are identical to those by ab initio, while the computing cost by the linear scaling SCC-DFTB is reduced by more than 30 times as compared with that by the Dmol for the (10,0) and (10,10) tubes. By computing the structure of a graphene it is also found that the linear scaling SCCDFTB is reliable and time-saving. 展开更多
关键词 Single walled nanotube tube Young's modulus Linear scaling method Density functional tight-binding
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基于能量法的隔震连续梁桥抗震加固研究
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作者 彭刚辉 姚国文 +3 位作者 郑史雄 姚运 贾宏宇 宋安祥 《铁道标准设计》 北大核心 2026年第1期71-78,共8页
隔震连续梁桥广泛应用于铁路及公路交通中,使用年限的增加及地震动作用导致桥墩抗震能力降低,桥墩加固对隔震连续梁桥地震响应的影响需进行系统研究。采用钢管作为加固材料,建立隔震连续梁桥有限元模型;依据不同的加固方案建立9种分析工... 隔震连续梁桥广泛应用于铁路及公路交通中,使用年限的增加及地震动作用导致桥墩抗震能力降低,桥墩加固对隔震连续梁桥地震响应的影响需进行系统研究。采用钢管作为加固材料,建立隔震连续梁桥有限元模型;依据不同的加固方案建立9种分析工况,选用与目标加速度反应谱相匹配的7条地震波,结合能量法进行非线性动力时程分析,对桥梁结构基本周期、滞回耗能、墩底弯矩、墩底剪力及隔震支座受力特性进行研究;选取隔震支座水平剪力及水平位移作为隔震效果评价指标,7条地震波作用平均值作为墩底弯矩及剪力的评价指标。研究结果表明:连续梁桥结构基本周期、隔震支座力学特性及体系滞回耗能与桥墩加固高度、加固墩数量具有相关性,加固高度较大时(H/2),其影响幅值范围为5.74%~20%,桥墩加固对墩底弯矩及剪力的影响幅值范围为1.86%~6.46%;在峰值加速度相同的情况下,地震波对加固后连续梁桥的滞回耗能影响幅值范围为6.88%~8.16%,而桥墩长径比对隔震支座受力特性有较大影响。研究成果可为既有隔震连续梁桥桥墩抗震加固提供借鉴。 展开更多
关键词 隔震连续梁桥 桥墩加固 钢管加固 能量法 滞回耗能 加固高度
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新型内置钢管补强混凝土开洞梁试验及理论研究
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作者 王勇 韩冠旭 +4 位作者 张亚军 张宝明 滕飞 龚成有 赵杰 《工程科学与技术》 北大核心 2026年第2期215-227,共13页
为便于混凝土梁洞口补强施工和优化传力机制,首先,提出新型内置钢管洞口补强方式,对混凝土开洞梁开展力学性能试验研究,获得不同补强方式对试件裂缝发展及分布、破坏模式、混凝土和钢筋应变及荷载-变形曲线等力学行为的影响规律;其次,基... 为便于混凝土梁洞口补强施工和优化传力机制,首先,提出新型内置钢管洞口补强方式,对混凝土开洞梁开展力学性能试验研究,获得不同补强方式对试件裂缝发展及分布、破坏模式、混凝土和钢筋应变及荷载-变形曲线等力学行为的影响规律;其次,基于ABAQUS软件,建立开洞梁数值模型,对试验梁内力机理进行对比分析;最后,提出附加压杆效应,建立开洞梁拉-压杆桁架分析模型,对试验梁抗剪承载力进行分析。结果表明:与传统斜筋补强方式相比,内置钢管洞口补强方式施工方便,采用内置钢管补强开洞梁,其极限承载力不低于同等工况下斜筋补强开洞梁,且有利于提高开洞梁抗剪承载力和减小梁底裂缝宽度;内置钢管补强方式在洞口区域形成附加压杆效应,缩短传力路径,所提拉-压杆桁架理论模型可用于分析内置钢管补强混凝土开洞梁抗剪承载力。本文所提出的内置钢管补强方式,有效解决了传统斜筋补强施工困难与受力性能较差等问题,为切实解决工程中开洞梁抗剪承载力不足与裂缝控制难的问题提供了兼具高效与可靠性的设计方法。 展开更多
关键词 开洞梁 补强方式 内置钢管 极限承载力 裂缝 主应力迹线
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船舶艉轴密封装置摩擦自激振动研究
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作者 邹冬林 薛林 +3 位作者 林强 杨宇泽 塔娜 饶柱石 《船舶力学》 北大核心 2026年第3期439-451,共13页
船舶艉轴密封装置在工作过程中常常因静环和动环间的摩擦激励而产生异常振动噪声,通过实船测试发现,该异常振动在振动频谱中表征为静环扭振固有频率及其倍频分量的出现。为揭示该异常振动产生原因,本文建立了摩擦激励下的艉轴密封装置... 船舶艉轴密封装置在工作过程中常常因静环和动环间的摩擦激励而产生异常振动噪声,通过实船测试发现,该异常振动在振动频谱中表征为静环扭振固有频率及其倍频分量的出现。为揭示该异常振动产生原因,本文建立了摩擦激励下的艉轴密封装置非线性扭振分析模型,利用多尺度法和数值仿真分别研究了轴系旋转速度、动静环间摩擦系数、Ω弹簧预紧力、Ω弹簧扭转刚度、系统结构阻尼比等参数对艉轴密封装置振动的影响规律。研究表明:当摩擦激励诱发的等效阻尼比小于系统的结构阻尼比时,艉轴密封装置的静环将产生摩擦自激振动,表现为振动频谱中出现静环扭振固有频率及其倍频分量;增大Ω弹簧预紧力、增大动静环间的静摩擦系数与动摩擦系数差异,或者降低轴系旋转速度、降低Ω弹簧扭转刚度均将导致静环更易发生摩擦自激振动;提高系统的结构阻尼比可有效抑制静环的摩擦自激振动;轴系扭振仅改变静环自激振动的幅值,而不会改变其性态。最后,本文提供了静环产生摩擦自激振动的判别公式。本文研究结果为实船艉轴密封装置的振动故障诊断、设计优化及运行调控提供理论依据与技术支撑。 展开更多
关键词 艉轴密封装置 自激振动 异常振动 多尺度法 摩擦激励 扭转振动
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Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin 被引量:2
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作者 ZHANG Shuzhen XU Pengfei +4 位作者 ZHANG Dayong LIN Shifeng LI Wenbin HAN Yingpeng YANG Chuanping 《Journal of Northeast Agricultural University(English Edition)》 CAS 2006年第1期7-10,共4页
Kanamycin was used to screen To seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg·L^-1 kanamycin could inhibit growth of non-transgenic plants, and 2 posi... Kanamycin was used to screen To seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg·L^-1 kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is economic and effective in preliminary screening the transgenic plants. 展开更多
关键词 SOYBEAN pollen-tube method KANAMYCIN
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桩内单孔管波法检测理论及其应用
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作者 付鹏程 吴君涛 +3 位作者 易恩泽 王奎华 朱旭峰 赵竹占 《岩土工程学报》 北大核心 2026年第3期539-548,共10页
近年来,单孔管波法可以通过竖向激振作用下桩内声测管中的水压响应时深规律来判断桩身完整性,但是该方法的检测理论尚不完善。因此,提出了桩周土-桩-测管内流体耦合振动模型,结合桩身三维振动控制方程以及Biot流体位移势函数控制方程,... 近年来,单孔管波法可以通过竖向激振作用下桩内声测管中的水压响应时深规律来判断桩身完整性,但是该方法的检测理论尚不完善。因此,提出了桩周土-桩-测管内流体耦合振动模型,结合桩身三维振动控制方程以及Biot流体位移势函数控制方程,求解得出测管中不同深度处的水压响应表达式。解得桩身振动响应与退化工况下的已有解几乎一致。基于验证后的解析解,开展测管中水压传播机理的研究。通过水压响应波列图,可观察到管中水压以两种波速进行传播,管中的水压响应是应力波沿桩身传播与管波在测管内传播共同作用的结果。对不同工况下的土-桩-测管系统进行参数分析,研究水压响应的影响因素。结合现场试验结果,证明了单孔管波法进行桩基完整性检测的合理性与有效性。研究结论能为单孔管波法检测技术的推广应用提供工程指导以及理论依据。 展开更多
关键词 桩基础 单孔管波法 动力响应 解析解 斯通利波
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卒中后吞咽障碍患者胃管置入方法及位置验证的研究进展
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作者 孙洋 姜永梅 《中国临床护理》 2026年第1期62-66,共5页
吞咽障碍是卒中后患者常见的并发症之一,严重影响患者摄食和生活质量。胃管置入作为一种有效的营养支持方式,其置入方法的安全性及位置验证的准确性对患者安全具有重要临床意义。文章对卒中后吞咽障碍特点、卒中后吞咽障碍患者胃管置入... 吞咽障碍是卒中后患者常见的并发症之一,严重影响患者摄食和生活质量。胃管置入作为一种有效的营养支持方式,其置入方法的安全性及位置验证的准确性对患者安全具有重要临床意义。文章对卒中后吞咽障碍特点、卒中后吞咽障碍患者胃管置入方法及位置验证等进行了综述,旨在为后续研究的开展及临床应用提供参考。 展开更多
关键词 卒中后吞咽障碍 胃管 置管方法 安全性 位置验证
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强渗透地层管井降水的滤料设计方法及应用
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作者 李达 刘宁毅 +3 位作者 赵耀威 焦雷 刘中欣 马建军 《水文地质工程地质》 北大核心 2026年第2期105-114,共10页
为避免富水强渗透地层工程降水引起地层细颗粒流失而导致环境问题,需建立一种合理确定降水管井的滤料粒径的设计方法。基于反滤层滤土与减压的工作原理,提出一种以有效层间系数为2~3和不均匀系数小于等于2为控制指标的管井降水滤料设计... 为避免富水强渗透地层工程降水引起地层细颗粒流失而导致环境问题,需建立一种合理确定降水管井的滤料粒径的设计方法。基于反滤层滤土与减压的工作原理,提出一种以有效层间系数为2~3和不均匀系数小于等于2为控制指标的管井降水滤料设计准则。通过离散元数值模拟,分析了渗流过程中地层-滤料系统的颗粒流失特性与孔隙率变化规律,揭示了滤料通过截留细粒达到自稳定状态的细观机制。结果表明:当有效层间系数小于等于3时,细粒流失量小于3%,且孔隙率趋于稳定。后续经过现场抽水试验验证了该滤料设计准则的有效性。本研究为管井降水滤料设计提供了理论依据,可提升富水强渗透地层基坑降水工程的安全性。 展开更多
关键词 强渗透地层 管井降水 滤料设计 有效层间系数 离散元模拟
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管壳式换热器管束排布多目标优化方法
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作者 金立杰 王艺烨 +3 位作者 李陈龙 许旭 王进峰 何雨彤 《应用化工》 北大核心 2026年第2期490-494,共5页
为了优化管壳式换热器的换热性能,采用灰度关联法对管束排布方式进行了优化研究。建立了5种不同排布方式的换热器物理模型并通过Fluent软件进行数值模拟,获得了换热器的进出口温度和压力变化数据;从温度场、压力场和流动特性等多个维度... 为了优化管壳式换热器的换热性能,采用灰度关联法对管束排布方式进行了优化研究。建立了5种不同排布方式的换热器物理模型并通过Fluent软件进行数值模拟,获得了换热器的进出口温度和压力变化数据;从温度场、压力场和流动特性等多个维度对换热方案进行对比分析,评估不同排布方式对换热性能和流动阻力的影响;通过灰度关联法确定了流体平均换热量、泵功和热量损失的权重并进行了多目标优化,最终确定转角正方形排列为最佳方案。研究结果表明:与其他排布方式相比,转角正方形排列在多目标优化下表现出较好的综合性能,能够平衡换热效果与能耗,提升换热器的整体运行稳定性。 展开更多
关键词 灰度关联法 管壳式换热器 管束排布 热量损失
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舰用管壳式换热器抗冲击性能计算方法研究
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作者 徐双喜 刘景强 +3 位作者 李培跃 金向东 任仲豪 谌伟 《武汉理工大学学报(交通科学与工程版)》 2026年第1期98-104,共7页
文中提出了一种等效替代的方法,并使用时域模拟法对换热器进行抗冲击仿真计算.将细换热管分别等效为14和26根粗换热管进行建模,对两种等效模型进行模态分析,得到了等效模型的振型及模态质量,采用时域模拟法对整体母模型开展仿真计算,最... 文中提出了一种等效替代的方法,并使用时域模拟法对换热器进行抗冲击仿真计算.将细换热管分别等效为14和26根粗换热管进行建模,对两种等效模型进行模态分析,得到了等效模型的振型及模态质量,采用时域模拟法对整体母模型开展仿真计算,最后,对换热管局部子模型开展单独计算,得到了换热器的冲击响应规律.结果表明:换热器各部位应力均满足相关规范的评估要求,采用粗换热管等效替代细换热管的方法能够有效的开展管壳式换热器的时域冲击仿真计算. 展开更多
关键词 管壳式换热器 抗冲击 等效替代 时域模拟法
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白鲜花粉形态及特性研究
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作者 宋占伟 张晴 +4 位作者 徐玲洁 郭冰柔 桑子涵 程顺 李彦慧 《草业科学》 北大核心 2026年第1期152-159,共8页
白鲜(Dictamnus dasycarpus)是河北省重要的野生花卉资源,其花多色艳,花型美观,具有极高的观赏价值。本研究以保定地区的白鲜为试验材料,通过扫描电子显微镜对其花粉形态进行了观察,并采用4种染色方法[四唑盐比色法(MTT法)、醋酸洋红、... 白鲜(Dictamnus dasycarpus)是河北省重要的野生花卉资源,其花多色艳,花型美观,具有极高的观赏价值。本研究以保定地区的白鲜为试验材料,通过扫描电子显微镜对其花粉形态进行了观察,并采用4种染色方法[四唑盐比色法(MTT法)、醋酸洋红、碘-碘化钾法(I_(2)-KI法)、2,3,5-三苯基氯化四氮唑法(TTC法)]测定其花粉活力和不同储存条件(25、-4、-20、-80℃)下花粉活力变化,以及荧光显微观察其花粉萌发过程等,探究白鲜花粉形态及花粉萌发特性。结果表明,白鲜花粉为长椭圆形,具3个萌发沟,沟达两级,极面呈三裂圆形。MTT法、醋酸洋红、I_(2)-KI法3种方法均能花粉进行染色,MTT法中有活力的花粉呈蓝紫色,无活力花粉呈无色,颜色区分明显,不同时间下花粉染色变化明显;在醋酸洋红法与I_(2)-KI法染色下,对比开花1 d和开花3 d的花粉染色情况无明显变化;TTC法对不同时间下花粉均不能染色;-80℃下储存60 d花粉活力为45.47%,延长了花粉寿命。对白鲜进行异株异花授粉,授粉后2 h,花粉在柱头表面萌发,在授粉后48 h,少量花粉管开始进入子房。综上,本研究结果增加了对白鲜花粉形态的认识和对花粉特性的了解,为进一步研究提供了参考。 展开更多
关键词 白鲜 花粉形态 花粉活力 花粉萌发 扫描电镜 检测方法 花粉管
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