目的探讨中国西南地区汉族人群中PRDM16、TRPM8、TSPAN2及MMP16等基因多态性与无先兆偏头痛遗传易感性关系。方法共收集512例无先兆偏头痛和535例健康正常对照,运用病例-对照分析,荟萃分析目前国内外报道的全基因组关联分析(genome-wide...目的探讨中国西南地区汉族人群中PRDM16、TRPM8、TSPAN2及MMP16等基因多态性与无先兆偏头痛遗传易感性关系。方法共收集512例无先兆偏头痛和535例健康正常对照,运用病例-对照分析,荟萃分析目前国内外报道的全基因组关联分析(genome-wide association studies,GWAS),单碱基延伸法(SNa Pshot)进行多个基因11个SNP位点的基因分型。结果 rs2651899(PRDM16),rs10166942(TRPM8),rs12134493(TSPAN2)和rs10504861(MMP16)与无先兆偏头痛发病具相关性。结论本研究重复了以前GWAS结果,PRDM16,TRPM8,TSPAN2,MMP16基因的SNP位点与无先兆偏头痛发病相关。展开更多
AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 ...AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 mRNA assay by RT-PCR.Eighty-eight specimens of human colorectal adenocarcinoma were surgically removed.TSPAN1 protein levels in cancer tissues were determined by immunohistochemistry using a polyclonal antibody against self-prepared TSPAN1.The correlation between TSPAN1 expression and the clinicopathological factors and the overall survival rate was analyzed by univariate and multivariate assay.RESULTS:TSPAN1 mRNA was detected in 90.0%(18/20) of cancerous tissues.The light density of TSPAN1 mRNA expression levels was 0.89 ± 0.30 in adenocarcinoma by gel-image system.TSPAN1 protein expression was detected in 78.41%(69/88) and weakly expressed in 40% normal colorectal tissues.There were significant differences between colorectal adenocarcinoma and normal control epithelium(P < 0.05).TSPAN1 protein expression in colorectal cancerous tissue was significantly correlated with the histological grade,cell expression PCNA,lymph nodal metastasis and TNM staging of the disease.Patients with TSPAN1 protein overexpression had a significantly shorter survival period than that in patients with TSPAN1 protein negative or weak expression,respectively(P < 0.05).Furthermore,by multivariate analysis,TSPAN1 protein expression demonstrated an independent prognostic factor for human colorectal cancers(P < 0.05,relative risk 0.755;95% confidence interval 0.302-1.208).CONCLUSION:The expression of TSPAN1 gene is increased in colorectal carcinoma,suggesting that TSPAN1 might serve as an independent prognostic factor for the colorectal adenocarcinoma patients.展开更多
文摘目的探讨中国西南地区汉族人群中PRDM16、TRPM8、TSPAN2及MMP16等基因多态性与无先兆偏头痛遗传易感性关系。方法共收集512例无先兆偏头痛和535例健康正常对照,运用病例-对照分析,荟萃分析目前国内外报道的全基因组关联分析(genome-wide association studies,GWAS),单碱基延伸法(SNa Pshot)进行多个基因11个SNP位点的基因分型。结果 rs2651899(PRDM16),rs10166942(TRPM8),rs12134493(TSPAN2)和rs10504861(MMP16)与无先兆偏头痛发病具相关性。结论本研究重复了以前GWAS结果,PRDM16,TRPM8,TSPAN2,MMP16基因的SNP位点与无先兆偏头痛发病相关。
基金Supported by The University High-New-Tech Development Fund of Jiangsu Province,No. JHO2-118Natural Science Foundation of Jiangsu Province,No.BK2006058National Natural and Science Foundation,No.30771126
文摘AIM:To determine if TSPAN1 overexpression is associated with clinicopathological and prognostic factors in human colorectal adenocarcinoma.METHODS:Total RNA was extracted in 20 human adenocarcinoma tissues for TSPAN1 mRNA assay by RT-PCR.Eighty-eight specimens of human colorectal adenocarcinoma were surgically removed.TSPAN1 protein levels in cancer tissues were determined by immunohistochemistry using a polyclonal antibody against self-prepared TSPAN1.The correlation between TSPAN1 expression and the clinicopathological factors and the overall survival rate was analyzed by univariate and multivariate assay.RESULTS:TSPAN1 mRNA was detected in 90.0%(18/20) of cancerous tissues.The light density of TSPAN1 mRNA expression levels was 0.89 ± 0.30 in adenocarcinoma by gel-image system.TSPAN1 protein expression was detected in 78.41%(69/88) and weakly expressed in 40% normal colorectal tissues.There were significant differences between colorectal adenocarcinoma and normal control epithelium(P < 0.05).TSPAN1 protein expression in colorectal cancerous tissue was significantly correlated with the histological grade,cell expression PCNA,lymph nodal metastasis and TNM staging of the disease.Patients with TSPAN1 protein overexpression had a significantly shorter survival period than that in patients with TSPAN1 protein negative or weak expression,respectively(P < 0.05).Furthermore,by multivariate analysis,TSPAN1 protein expression demonstrated an independent prognostic factor for human colorectal cancers(P < 0.05,relative risk 0.755;95% confidence interval 0.302-1.208).CONCLUSION:The expression of TSPAN1 gene is increased in colorectal carcinoma,suggesting that TSPAN1 might serve as an independent prognostic factor for the colorectal adenocarcinoma patients.
