Acute respiratory distress syndrome(ARDS)is a common respiratory emergency,but current clinical treatment remains at the level of symptomatic support and there is a lack of effective targeted treatment measures.Our pr...Acute respiratory distress syndrome(ARDS)is a common respiratory emergency,but current clinical treatment remains at the level of symptomatic support and there is a lack of effective targeted treatment measures.Our previous study confirmed that inhalation of hydrogen gas can reduce the acute lung injury of ARDS,but the application of hydrogen has flammable and explosive safety concerns.Drinking hydrogen-rich liquid or inhaling hydrogen gas has been shown to play an important role in scavenging reactive oxygen species and maintaining mitochondrial quality control balance,thus improving ARDS in patients and animal models.Coral calcium hydrogenation(CCH)is a new solid molecular hydrogen carrier prepared from coral calcium(CC).Whether and how CCH affects acute lung injury in ARDS remains unstudied.In this study,we observed the therapeutic effect of CCH on lipopolysaccharide(LPS)induced acute lung injury in ARDS mice.The survival rate of mice treated with CCH and hydrogen inhalation was found to be comparable,demonstrating a significant improvement compared to the untreated ARDS model group.CCH treatment significantly reduced pulmonary hemorrhage and edema,and improved pulmonary function and local microcirculation in ARDS mice.CCH promoted mitochondrial peripheral division in the early course of ARDS by activating mitochondrial thioredoxin 2(Trx2),improved lung mitochondrial dysfunction induced by LPS,and reduced oxidative stress damage.The results indicate that CCH is a highly efficient hydrogen-rich agent that can attenuate acute lung injury of ARDS by improving the mitochondrial function through Trx2 activation.展开更多
[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the ...[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.展开更多
基金supported by the Nanjing Medical Science and Technology Development Project,China(Project No.:YKK23221)Open Project of Immune Cell Translational Research Center of Jiangning Hospital,Nanjing Medical University,China(Project No.:JNYYZXKY202216)+1 种基金General Project of Medical Education Collaborative Innovation Fund of Jiangsu University,China(Project No.:JDYY2023094)China Red Cross Foundation Medical Empowerment Charity Special Fund Project(Project No.:CRCF-YXFN-202302028).
文摘Acute respiratory distress syndrome(ARDS)is a common respiratory emergency,but current clinical treatment remains at the level of symptomatic support and there is a lack of effective targeted treatment measures.Our previous study confirmed that inhalation of hydrogen gas can reduce the acute lung injury of ARDS,but the application of hydrogen has flammable and explosive safety concerns.Drinking hydrogen-rich liquid or inhaling hydrogen gas has been shown to play an important role in scavenging reactive oxygen species and maintaining mitochondrial quality control balance,thus improving ARDS in patients and animal models.Coral calcium hydrogenation(CCH)is a new solid molecular hydrogen carrier prepared from coral calcium(CC).Whether and how CCH affects acute lung injury in ARDS remains unstudied.In this study,we observed the therapeutic effect of CCH on lipopolysaccharide(LPS)induced acute lung injury in ARDS mice.The survival rate of mice treated with CCH and hydrogen inhalation was found to be comparable,demonstrating a significant improvement compared to the untreated ARDS model group.CCH treatment significantly reduced pulmonary hemorrhage and edema,and improved pulmonary function and local microcirculation in ARDS mice.CCH promoted mitochondrial peripheral division in the early course of ARDS by activating mitochondrial thioredoxin 2(Trx2),improved lung mitochondrial dysfunction induced by LPS,and reduced oxidative stress damage.The results indicate that CCH is a highly efficient hydrogen-rich agent that can attenuate acute lung injury of ARDS by improving the mitochondrial function through Trx2 activation.
基金Supported by Major Innovation Program for Extraction of Plant-derived NaturalDisease-resistant Components and Industrialization(KX140564)
文摘[ Objective ] The study was to explore whether glutaredoxin （GRX2） and thioredoxin （TRX2） genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [ Methods ] Using Sanhuang chicken as the control, the GRX2 and TRX2 genes were cloned from skirt tissues of Puerp/ao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [ Results ] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerp/ao chicken, Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in GenBank, and no any mutation was observed. [ Conclusion] The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.
