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3株木霉(Trichoderma spp.)对华山松疱锈病菌锈孢子的破坏作用 被引量:15
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作者 陈玉惠 杨艳红 +1 位作者 李永和 林宏益 《植物保护》 CAS CSCD 北大核心 2006年第6期62-65,共4页
从不同来源的茶藨生柱锈锈孢子堆和疱锈病枝干上分离获得3株木霉,室内生测结果表明,3株木霉的菌丝和培养滤液对锈孢子壁有较强的破坏作用;菌株TR2和TR3在锈孢子堆上生长速度快,锈孢子死亡率随孢子壁破坏率的增加而升高,锈孢子壁对孢壁... 从不同来源的茶藨生柱锈锈孢子堆和疱锈病枝干上分离获得3株木霉,室内生测结果表明,3株木霉的菌丝和培养滤液对锈孢子壁有较强的破坏作用;菌株TR2和TR3在锈孢子堆上生长速度快,锈孢子死亡率随孢子壁破坏率的增加而升高,锈孢子壁对孢壁降解酶有强烈诱导作用;TR1对锈孢子壁的破坏作用弱于TR2和TR3,但在PD培养液中有较强的产毒能力,锈孢子壁能抑制TR1菌株产毒。 展开更多
关键词 木霉 锈孢子 生物防治 华山松疱锈病
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Trichoderma spp.对樟子松枯梢病病原菌的影响 被引量:14
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作者 宋瑞清 周秀华 《东北林业大学学报》 CAS CSCD 北大核心 2004年第4期29-30,共2页
通过Trichoderma属 3个菌株与樟子松枯梢病菌 (Sphaeropsissapinea)对峙培养试验的结果表明 :Tri chodermaviride1,Trichodermaviride 2及Trichodermaharzianum对樟子松枯梢病病原菌都有抑制效果 ,其中Trichodermaviride 2对病原菌的相... 通过Trichoderma属 3个菌株与樟子松枯梢病菌 (Sphaeropsissapinea)对峙培养试验的结果表明 :Tri chodermaviride1,Trichodermaviride 2及Trichodermaharzianum对樟子松枯梢病病原菌都有抑制效果 ,其中Trichodermaviride 2对病原菌的相对抑制效果最好 ,且其相对抑制效果随着时间的增加而增长 ,在 4 0h达到最高 ,为 73.5 5。Trichodermaviride 1和Trichodermaharzianum对病原菌的相对抑制效果在 16h达到最高 ,分别为 4 .86及 2 .5 9。 展开更多
关键词 trichoderma spp Sphaeropsis sapinea 拮抗作用
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具有生防能力的木霉菌(Trichoderma spp.)与两株大豆根腐病病原菌(Fusarium oxysporum、Rhizoctonia solani)对碳、磷、铁的竞争研究 被引量:7
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作者 邵红涛 许艳丽 《黑龙江大学自然科学学报》 CAS 北大核心 2007年第1期126-129,共4页
通过室内试验研究比较了具有生物防治能力的5株木霉菌株与2株大豆根腐病病原菌(Fusarium oxysporum、Rhizoctonia solani)对C、P、Fe等营养元素的竞争。试验结果表明病原菌F.oxysporum利用葡萄糖的速度显著的高于所测试的木霉菌株(菌株... 通过室内试验研究比较了具有生物防治能力的5株木霉菌株与2株大豆根腐病病原菌(Fusarium oxysporum、Rhizoctonia solani)对C、P、Fe等营养元素的竞争。试验结果表明病原菌F.oxysporum利用葡萄糖的速度显著的高于所测试的木霉菌株(菌株MM9除外),而木霉菌株MM9、S7、SH7、S2利用葡萄糖的速率显著的高于病原菌R.solani。木霉菌株S7、S2利用可溶性磷的速率显著的高于病原菌F.oxysporum。木霉菌株MM35利用可利用铁的速率显著的高于病原菌(F.oxysporum、R.solani)。木霉菌与大豆根腐病病原菌(F.oxysporum、R.solani)对C、P、Fe营养的竞争呈现多样性。 展开更多
关键词 木霉菌 大豆根腐病 机制 竞争
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Inhibition of Chlamydospore Germination and Mycelial Growth of Trichoderma spp. by Chemical Fungicides 被引量:2
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作者 张林 蒋细良 +2 位作者 杨晓燕 李梅 陈书华 《Agricultural Science & Technology》 CAS 2015年第7期1494-1499,共6页
For exploring the influences of application and residue of chemical fungi- cides on chlamydospore preparations of Trichoderma spp., the effects of seven chemical fungicides on chlamydospore germination and mycelia gro... For exploring the influences of application and residue of chemical fungi- cides on chlamydospore preparations of Trichoderma spp., the effects of seven chemical fungicides on chlamydospore germination and mycelia growth of two bio- control fungi T. harzianum 610 and T. Iongibrachiatum 758 were studied. Carben- dazim, tebuconazole and difenoconazole showed strong toxicities, thiram and car- bexin showed moderate toxicities, and metalaxyl showed Mycelia growth of the two strains was more sensitive to most tested fungicides than those of chlamydospore germination. Chlamydospore germination of 610 was more sensitive to tested fungicides than those of 758, and mycelia growth of 758 was more sensitive to most tested fungi- cides than those of 610. Among the seven fungicides, 98% carbendazim had the strongest effects (ECru values were 1.64 and 0.05μpg/ml), and 70% pentachloroni- trobenzene had the weakest effects (EC50 values were 1.64 and 0,05 μg/ml) for chlamydospore germination and mycelia growth of 610. As for 758, 98% carbendaz- im had the strongest inhibitory effects and 95% metalaxyl had the weakest inhibitory for chlamydospore germination of 756 (EC50 values were 0.62 and 1 108.61 μg/ml respectively), whereas 96.2% tebuconazole showed the strongest inhibitory effects for mycelia growth of 758 (EC= value was 0.32μg/ml), and 95% metalaxyl was the weakest (EC= value was 1 206.29 μg/ml). According to the applied concentration of different fungicides in practice, we concluded that chlamydospore preparations of 610 and 758 could not be combined with carbendazim, tebuconazole, thiram and carboxin for controlling plant diseases, and the pesticide residues to the biocontrol effects should be kept in mind. Chlamydospore preparations of 610 and 758 can be and difenoconazole for controlling plant dis- eases, 758 chlamydospore preparations and germinated chlamydospore of 610 can be combined with metalaxyl for controlling plant diseases, and pesticide residue risk was not serious. 展开更多
关键词 Chemical fungicide INHIBITION trichoderma spp Chlamydospore germi- nation Mycelia growth
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木霉(Trichoderma spp.)对三种引起大棚蔬菜病害病原菌的影响 被引量:7
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作者 宋瑞清 周秀华 Sirajul HASAH 《菌物研究》 CAS 2004年第4期6-10,共5页
通过木霉属(Trichoderma) 3菌株与双鸭山蔬菜大棚中的黄瓜枯萎病菌(FusariumoxysporumSchlecht.f.cucumerinum)、黄瓜果腐病菌(PhytophthoracapsiciLeonian)、菜豆叶枯病菌(Cladosporiumsp .)的对峙培养试验,结果表明:绿色木霉1(Trichod... 通过木霉属(Trichoderma) 3菌株与双鸭山蔬菜大棚中的黄瓜枯萎病菌(FusariumoxysporumSchlecht.f.cucumerinum)、黄瓜果腐病菌(PhytophthoracapsiciLeonian)、菜豆叶枯病菌(Cladosporiumsp .)的对峙培养试验,结果表明:绿色木霉1(TrichodermaviridePers.exGray 1)可作为双鸭山蔬菜大棚中的黄瓜枯萎病、黄瓜果腐病、菜豆叶枯病3种病害的生物防治拮抗菌加以利用,该拮抗菌对菜豆叶枯病菌抑制效果最好;绿色木霉2 (Tricho dermaviride 2 )对黄瓜果腐病菌抑制效果最好;而哈茨木霉(TrichodermaharzianumRifai)对以上3种病原菌都有抑制效果,对菜豆叶枯病菌抑制效果最好。从试验结果还可看出,绿色木霉2对黄瓜枯萎病菌和菜豆叶枯病菌的生长有促进作用。 展开更多
关键词 木霉 黄瓜枯萎病菌 黄瓜果腐病茵 菜豆叶枯病菌 拮抗作用
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Effects of Penicillium spp. and Trichoderma spp.on Pleurotus ostreatus Growth and Screening of Effective Disinfectants 被引量:2
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作者 覃培升 杨琴 +2 位作者 黄福常 刘斌 黎金锋 《Agricultural Science & Technology》 CAS 2015年第3期435-438,473,共5页
[Objective] This study was conducted to investigate the effects of Penicillium spp. and Trichoderma spp. on the growth of Pleurotus ostreatus (oyster mushroom) and to screen effective disinfectants to control them a... [Objective] This study was conducted to investigate the effects of Penicillium spp. and Trichoderma spp. on the growth of Pleurotus ostreatus (oyster mushroom) and to screen effective disinfectants to control them and other pathogenic microbes. [Method] Six strains or species of Penicilliurn and Trichoderma were inocu- lated into PDA plates growing with P. ostreatus to observe the growth of their mycelia. And the inhibitory effects of hydrogen peroxide, bromogeramine bromide, 84 disinfectant, lysol, potassium permanganate, and 75% medical alcohol on Trichoderma pleuroticola, Trichoderma viride, Trichoderma harzianum, Penicillium citrinum, Penicillium thomii and Penicillium brevicompactum were detected. [Result] Confrontation test revealed that Penicillium spp. and Trichoderma spp. competed for nutrients with P. ostreatus mycelia, antagonized against the growth of P. ostreatus mycelia, invaded and twined around P. ostreatus mycelia, and also produced toxins poisoning P. ostreatus mycelia. The six disinfectants exhibited different inhibitory effects against different species of Penicillium and Trichoderma. Among them the in- hibitory effects of bromogeramine bromide against the six species of Penicillium and Trichoderma were significantly different. However, the inhibitory effects of 3% hydrogen peroxide, 84 disinfectant, 5% lysol, potassium permanganate and 75% medical alcohol were significantly different among Penicillium spp., but did not among Tri- choderma spp. [Conclusion] Bromogeramine bromide can be used to control the bacteria and fungi in mushroom production for it has significant inhibitory effects on the six species of both Penicillium and Trichoderma. 展开更多
关键词 PENICILLIUM trichoderma Pleurotus ostreatus DISINFECTANT
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Advances in biocontrol mechanism and application of Trichoderma spp.for plant diseases 被引量:1
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作者 HUANG Caihong YANG Qian 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第2期161-167,共7页
Trichoderma spp. is a filamentous soil fungus known as an effective biocontrol agent of a range of important airborne and soilborne pathogens, it has universal distribution and economic importance. This article review... Trichoderma spp. is a filamentous soil fungus known as an effective biocontrol agent of a range of important airborne and soilborne pathogens, it has universal distribution and economic importance. This article reviewed the researches on biocontrol mechanism for plant diseases and application of Trichoderma spp., especially Trichoderma harzianum in recent years 展开更多
关键词 trichoderma spp. trichoderma harzianum biocontrol mechanism APPLICATION
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木霉(Trichoderma spp.)对植物土传病害生防机制的研究进展 被引量:20
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作者 邹佳迅 范晓旭 宋福强 《大豆科学》 CAS CSCD 北大核心 2017年第6期970-977,共8页
应用木霉防治植物土传病害已经成为目前生物防治研究的热点。随着分子生物学与生物工程等技术的不断发展,对木霉防治病原微生物的生防机制研究已经取得较大的进展。本文主要从竞争作用、重寄生作用、抗生作用、诱导抗性、促生作用、协... 应用木霉防治植物土传病害已经成为目前生物防治研究的热点。随着分子生物学与生物工程等技术的不断发展,对木霉防治病原微生物的生防机制研究已经取得较大的进展。本文主要从竞争作用、重寄生作用、抗生作用、诱导抗性、促生作用、协同拮抗等方面对木霉的生防机制进行综述,并阐述了防病的分子机理,以期使木霉更加合理地应用到土传病害防治领域中。同时,文章也对木霉的实际应用与可能出现的问题进行分析,并对今后的研究工作进行展望。 展开更多
关键词 木霉 土传病害 生物防治 生防机制 分子机理
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New advances in the science and use of Trichoderma spp. 被引量:1
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作者 Gary Harman 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2004年第4期388-388,共1页
Trichoderma spp. have been known for their strong abilities to produce extracellular enzymes, especially cellulases and glucanases by T. reesei, and also for their abilities to control plant pathogens. Most notably, a... Trichoderma spp. have been known for their strong abilities to produce extracellular enzymes, especially cellulases and glucanases by T. reesei, and also for their abilities to control plant pathogens. Most notably, a number of lines of evidence indicate that the agriculturally relevant strains are synergistic plant symbionts. As plant symbionts they possess the following characteristics: (a) they infect plant roots but cause the plant to restrict their growth to outer layers of the plant cortex (an exception is the recent discoveries of plant endophytic strains of Trichoderma and Gliocladium ), (b) they produce bioactive plant molecules in the zone of interaction that (c) induce localized systemic resistance to plant diseases and (d) systemic plant resistance to diseases that in some respects is similar to that induced by rhizobacteria, (e) they induce changes in plant proteomes, (f) increase plant growth and yield at least in part by enhancing root growth, and (g) they increase plant nutrient uptake. The increase in plant growth and yields is strongly interactive with plant genotype, at least in maize. There also are very significant increases in the knowledge of events that occur in mycoparasitism that in many respects have features in common with their interactions with plants. They are highly resistant to toxicants and have recently been shown to degrade cyanide and take up, and then degrade, metallocyanides. These discoveries allow new uses for the organisms in managed plant systems, including agriculture. For example, the induced systemic resistance system allows control of fungi, bacteria, Oomycetes and even one virus at sites temporally and spatially distant from the site of application. The abilities of the fungi to resist/degrade toxicants and increase plant growth indicate they will highly useful components in plant-microbe or microbe-only strategies for remediation of soil and water pollution. Finally, there are new uses of their enzyme systems for production of chitin degradation products for use as nutraceuticals or pharmaceuticals. 展开更多
关键词 植物 抗性 木霉素 真菌 相互作用 寄生
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Biocontrol Endotherapy with Trichoderma spp. and Bacillus amyloliquefaciens against Phytophthora spp.: A Comparative Study with Phosphite Treatment on Quercus robur and Fagus sylvatica
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作者 Gilles Berger Katarzyna Czarnocka +2 位作者 Bastien Cochard Tomasz Oszako Francois Lefort 《Journal of Agricultural Science and Technology(A)》 2015年第6期428-439,共12页
Phytophthora species are particularly aggressive plant pathogens and are often associated with the decline of many tree species, including oak and beech. Several fungi and bacteria species are known as potential antag... Phytophthora species are particularly aggressive plant pathogens and are often associated with the decline of many tree species, including oak and beech. Several fungi and bacteria species are known as potential antagonists usable as biological control agents. Phosphonate (H3PO3), commonly branded as phosphite, has also been used in the past years to protect trees against invasive Phytophthora spp.. This study aimed at comparing the effects of selected antagonist microorganisms and phosphonate, when applied by microinjection or leaf treatment. Antagonistic species were first selected for their high inhibitory activity against problematic Phytophthora species, such as Phytophthora cactorum, P. quercina and P. plurivora attacking Quercus robur and Fagus sylvatica in Polish forests. Three endophytic species Trichoderma atroviride (two strains), T. harzianum and Bacillus amyloliquefaciens showed a high control activity, and their efficacy was then assessed in comparison with a phosphonate treatment. Two application methods were experimented in this study: injection of a solution of spores or phosphonate into the sap vessels of beech or a foliar treatment on oak. Phosphonate and two strains of Trichoderma significantly reduced the necrotic area on oak leaves inoculated with P. plurivora and one strain of T. atroviride significantly reduced necrotic areas on beech branches. These results are therefore promising of a novel way to control Phytophthora spp. in forest stands and nurseries. 展开更多
关键词 Phytophthora spp. endotherapy trichoderma Bacillus sp. endophytes.
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The Beneficial Effect of Trichoderma spp. in Seed Treatment of Four Maize (Zea mays L.) Genotypes
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作者 José Luis Arispe-Vázquez Abiel Sánchez-Arizpe +7 位作者 Daniel Alejandro Cadena-Zamudio Ma. Elizabeth Galindo-Cepeda David Heriberto Noriega-Cantú Oscar Guadalupe Barrón-Bravo Leslie Carnero-Avilés Juan Mayo-Hernández Susana Elizabeth Ramírez-Sánchez Adriana Antonio-Bautista 《American Journal of Plant Sciences》 2023年第6期625-637,共13页
Maize is the main crop for Mexicans;however, it is affected by species of fungi causing ear rot. This research aimed to evaluate the effect of T. asperellum T11, T. harzianum T1 4 y T. longibrachiatum T1 40 on some ag... Maize is the main crop for Mexicans;however, it is affected by species of fungi causing ear rot. This research aimed to evaluate the effect of T. asperellum T11, T. harzianum T1 4 y T. longibrachiatum T1 40 on some agronomic variables of four maize genotypes. The seeds of the genotypes H-515, Zapata 7, and H-507 were treated with a suspension of Trichoderma spp. to 1 × 10<sup>8</sup> spores mL<sup>-1</sup>, using a control (untreated seed), and Benomyl as chemical control. The planting was in Morelos, in a completely random block design with a factorial arrangement. The ear rot was natural. Data were obtained at the end of the crop cycle and processed in SAS 9.4<sup><sup>®</sup></sup>. H-515 genotype had the greatest effect on the treatment of maize seeds with Trichoderma spp. (5.562 kg);T. asperellum T11 was the strain that stood out with a mean yield of 50 ears in an area of 16 m<sup>2</sup> of 4.904 kg, and control of 4.448 kg. Our results are an economic option for farmers to contemplate the use of Trichoderma and obtain its benefits. 展开更多
关键词 SEEDS TREATMENT trichoderma asperellum Ear Rot YIELD
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Trichoderma spp.and Bacillus spp.as growth promoters in maize(Zea mays L.)
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作者 López-Valenzuela BE Armenta-Bojórquez AD +3 位作者 Hernández-Verdugo S Apodaca-Sánchez MA Samaniego-Gaxiola JA Valdez-Ortiz A 《Phyton-International Journal of Experimental Botany》 2019年第1期37-46,共10页
Microbes that are beneficial to plants are used to enhance the crop growth,yield and are alternatives to chemical fertilizers.Trichoderma and Bacillus are the predominant plant growth-promoting fungi and bacteria.The ... Microbes that are beneficial to plants are used to enhance the crop growth,yield and are alternatives to chemical fertilizers.Trichoderma and Bacillus are the predominant plant growth-promoting fungi and bacteria.The objective of this study was select,characterize,and evaluate isolates of Trichoderma spp.and Bacillus spp.native from the northern region of Sinaloa,Mexico,and assess their effect on growth promotion in maize(Zea mays L.).In greenhouse conditions,four Trichoderma isolates and twenty Bacillus isolates,as well as two controls,were tested in a completely randomized design with three replicates.We selected the two best strains of Trichoderma and Bacillus:TB=Trichoderma asperellum,TF=Trichoderma virens,B14=Bacillus cereus sensu lato and B17=Bacillus cereus,which were evaluated in the field in a completely randomized blocks in factorial arrangement design with three replicates applying different rates of nitrogen fertilizer(0,150 kg N/ha,and 300 kg N/ha).Treatments 5(B17=B.cereus)and 11(TF=T.virens)both fertilized with 150 kg N/ha showed similar yields and they did not reveal significant differences from the treatments fertilized with 300 kg N/ha.This indicated that treatment 5(B17=B.cereus with 150 kg N/ha)and treatment 11(TF=T.virens with 150 kg N/ha)were efficient as growth promoters,by not showing significant differences in root volume and dry weight of foliage.The results indicated a reduction of 50% in the rate of nitrogen to fertilizer required for maize(Zea mays L.)crops.These microorganisms Trichoderma and Bacillus could be an alternative to reduce the use of chemical fertilizers in maize. 展开更多
关键词 trichoderma BACILLUS Native strains BIOFERTILIZERS
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Biocontrol of Rhizoctonia solani with Trichoderma Spp.
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作者 TANG Jiabin MA Bingtian WANG Lingxia LI Ping CHEN Hong Rice Res Inst,Sichuan Agri Univ,Wenjiang 611130,China 《Chinese Rice Research Newsletter》 2001年第4期8-8,共1页
From over 800 fungal strains of Tri-choderma Spp. , 6 strains were foundto greatly inhibit the growing of Rhi-zocotonia solani, the pathogen of ricesheath blight in dual culture. Amongthem, strain T3 was the best anta... From over 800 fungal strains of Tri-choderma Spp. , 6 strains were foundto greatly inhibit the growing of Rhi-zocotonia solani, the pathogen of ricesheath blight in dual culture. Amongthem, strain T3 was the best antago-nist, which reduced the growing ofthe pathogen by 52.54% (Table 1). 展开更多
关键词 Biocontrol of Rhizoctonia solani with trichoderma spp
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SMARCA4缺失型非小细胞肺癌的SPP1表达及其与PD-L1的关系 被引量:2
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作者 吴娟 黄曦 +5 位作者 李佳嘉 魏雨晴 张丽琴 俞咏梅 陆志伟 张鹤 《中国临床药理学与治疗学》 北大核心 2025年第4期477-486,共10页
目的:分析SMARCA4缺失型非小细胞肺癌中分泌性磷蛋白1(SPP1)及细胞程序性死亡-配体1(PD-L1)的表达,为罕见病理类型肺癌后续治疗研究提供科学依据。方法:回顾性分析12例SMARCA4缺失型非小细胞肺癌患者的临床与病理特征,根据其形态学分为... 目的:分析SMARCA4缺失型非小细胞肺癌中分泌性磷蛋白1(SPP1)及细胞程序性死亡-配体1(PD-L1)的表达,为罕见病理类型肺癌后续治疗研究提供科学依据。方法:回顾性分析12例SMARCA4缺失型非小细胞肺癌患者的临床与病理特征,根据其形态学分为腺癌与低分化癌两组,分析SPP1与PD-L1在两组的表达与关系。结果:所有患者均检出SPP1表达且其在低分化癌组表达水平较腺癌组显著升高(P=0.015);PD-L1表达者占6/7(5例未测),较之腺癌组,PD-L1亦在低分化癌组高表达(P=0.048),两组的PD-L1表达差异与SPP1表达差异一致。结论:SMARCA4缺失型非小细胞肺癌具有较高的SPP1及PD-L1阳性表达,病理形态为低分化癌者更甚,SPP1与PD-L1在SMARCA4缺失型非小细胞肺癌中的表达情况可能具有正相关性,其关联机制及免疫治疗中作用有待后续研究进一步探讨。 展开更多
关键词 NSCLC SMARCA4缺失 spp1 PD-L1 免疫治疗
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BDSBAS和WAAS对单频SPP精度增强对比分析
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作者 陈红英 陈韵陶 《全球定位系统》 2025年第3期82-87,共6页
星基增强系统(Satellite-Based Augmentation System,SBAS)在民航等精密定位领域的重要性逐渐凸显,但目前尚缺乏针对北斗星基增强系统(BeiDou Satellite-Based Augmentation System,BDSBAS)和广域增强系统(Wide Area Augmentation Syste... 星基增强系统(Satellite-Based Augmentation System,SBAS)在民航等精密定位领域的重要性逐渐凸显,但目前尚缺乏针对北斗星基增强系统(BeiDou Satellite-Based Augmentation System,BDSBAS)和广域增强系统(Wide Area Augmentation System,WAAS)在单频单点定位(single point positioning,SPP)应用中的性能差异对比,因此本文深入分析了SBAS对单频定位的精度增强.文章通过使用中国境内和北美地区的国际GNSS服务组织(International GNSS Service,IGS)多系统GNSS实验(Multi-GNSS Experiment,MGEX)数据,对比分析了BDSBAS和WAAS地球静止轨道(geostationary orbit,GEO)卫星的单频SPP定位效果,探讨了不同增强策略对轨道、钟差和电离层延迟的改正,验证了SBAS对定位精度的提升效果.结果显示,在各测站中,WAAS的表现优于BDSBAS,对均方根(root mean square,RMS)的最大改善达到60%,而BDSBAS在中国及周边地区也具有良好的适应性,对RMS的最大改善能达到50%,结合轨道、钟差和电离层延迟改正的策略,特别是SF_SBAS_ORB_ION在定位精度上表现最佳,在电离层影响显著的条件下,显著减少了误差并提高了定位稳定性.文章为SBAS在单频精密定位中的应用提供了新的数据支撑和参考. 展开更多
关键词 星基增强系统(SBAS) 定位增强 单频单点定位(spp) 轨道误差 电离层延迟
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SPP1在代谢相关性脂肪性肝炎中的诊断价值及调控机制
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作者 李沙 李锦忠 +3 位作者 谌宁 李敏然 戴二黑 齐亚娟 《暨南大学学报(自然科学与医学版)》 北大核心 2025年第4期505-517,共13页
目的:代谢相关性脂肪性肝炎(metabolic dysfunction-associated steatohepatitis,MASH)是代谢相关性脂肪性肝病(metabolic dysfunction-associated fatty liver disease,MAFLD)的进展阶段,目前主要依赖肝活检进行确诊,存在创伤性强、依... 目的:代谢相关性脂肪性肝炎(metabolic dysfunction-associated steatohepatitis,MASH)是代谢相关性脂肪性肝病(metabolic dysfunction-associated fatty liver disease,MAFLD)的进展阶段,目前主要依赖肝活检进行确诊,存在创伤性强、依从性差等问题。因此,亟需开发非侵入性生物标志物以辅助诊断。本研究旨在通过整合机器学习算法与肝脏转录组数据,筛选MASH关键诊断基因,并探讨分泌型磷蛋白1(secreted phosphoprotein 1,SPP1)在MASH中的调控机制及诊断价值。方法:从基因表达综合数据库(gene expression omnibus,GEO)获取MAFLD患者肝脏转录组数据集,通过差异表达分析筛选差异表达基因(differentially expressed genes,DEGs)。结合随机森林、人工神经网络、Lasso回归和支持向量机递归特征消除算法筛选关键基因,构建Neural-MASH诊断模型,并通过受试者工作特征(receiver operating characteristic,ROC)曲线评估性能。进一步分析候选关键基因与免疫细胞浸润及临床指标的相关性,并进行功能富集分析。结果:共筛选出85个DEGs,功能富集显示其与p53信号通路和细胞外基质(extra cellular matrix,ECM)-受体相互作用密切相关。通过多算法交叉验证,确定SPP1、含Fc受体样及黏蛋白结构域蛋白(fc alpha and mu receptor,FCAMR)、含黄素单加氧酶1(flavin-containing monooxygenase 1,FMO1)为关键基因,其中SPP1在MASH中表达上调,且与B细胞、巨噬细胞M0浸润及临床指标呈正相关(均P<0.05)。Neural-MASH模型在训练集和验证集中的曲线下面积(area under curve,AUC)分别为1.000和0.811。功能分析揭示SPP1主要参与ECM、细胞迁移调控、脂质定位及IL-18信号通路等生物学过程。结论:SPP1可作为MASH的潜在诊断标志物,其与免疫微环境的交互作用可能在疾病进展中发挥关键调控作用。基于机器学习所构建的Neural-MASH模型具有较高的诊断效能,可为MASH的无创诊断提供参考依据。 展开更多
关键词 代谢相关性脂肪性肝炎 分泌型磷蛋白1(spp1) 人工神经网络 生物标志物
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SPP1^(+)巨噬细胞及其表达产物CCL18在CTD-ILD中的水平及临床意义
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作者 张琳 庞春艳 +1 位作者 王永福 鲁芙爱 《包头医学院学报》 2025年第6期49-55,共7页
目的:分析结缔组织病合并间质性肺病(CTD-ILD)患者外周血中SPP1^(+)巨噬细胞的比例和其表达产物趋化因子18(CCL18)在CTD-ILD患者血清中表达水平及临床意义。方法:选取20例结缔组织病(CTD)患者和20例健康人群作为对照组,35例初诊CTD-ILD... 目的:分析结缔组织病合并间质性肺病(CTD-ILD)患者外周血中SPP1^(+)巨噬细胞的比例和其表达产物趋化因子18(CCL18)在CTD-ILD患者血清中表达水平及临床意义。方法:选取20例结缔组织病(CTD)患者和20例健康人群作为对照组,35例初诊CTD-ILD患者作为实验组,比较实验组与对照组中SPP1^(+)巨噬细胞、CCL18表达水平的差异,并与Ⅰ型胶原蛋白(COL1A1)、红细胞沉降率(ESR)、C-反应蛋白(CRP)等指标进行相关性分析。结果:与对照组相比,CTD-ILD患者外周血单核细胞中SPP1^(+)巨噬细胞比例显著增加(P<0.05),血清中CCL18的含量也明显升高(P<0.05);同时SPP1^(+)巨噬细胞、CCL18与ESR、CRP、COL1A1、肺部影像学评分(HRCT评分)呈正相关关系(P<0.05),与用力肺活量占预计值的百分比(FVC%)、一氧化碳弥散量占预计值的百分比(DLCO%)呈负相关关系(P<0.05);SPP1^(+)巨噬细胞和CCL18的曲线下面积(AUC)分别为0.732、0.939,二者联合检测的AUC为0.939,灵敏度为93.5%,特异度为83.3%,约登指数为0.768。结论:SPP1^(+)巨噬细胞及CCL18是CTD-ILD患者预后不良的因素,二者联合检测对疾病的预后具有重要的意义。 展开更多
关键词 结缔组织病合并间质性肺病 spp1^(+)巨噬细胞 趋化因子18 Ⅰ型胶原
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Trichoderma vermifimicola strain TC467 possessing phytopathogen antagonism,plant growth promotion and fungicide resistance
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作者 WANG Hengxu ZENG Zhaoqing ZHUANG Wenying 《菌物学报》 北大核心 2025年第10期102-113,共12页
Trichoderma strains possessing biological control functions have been used in agriculture against phytopathogens.Currently,only very few species of the genus were applied to or involved in plant disease control.Discov... Trichoderma strains possessing biological control functions have been used in agriculture against phytopathogens.Currently,only very few species of the genus were applied to or involved in plant disease control.Discovery of additional useful resources is desperately needed.In this study,biocontrol effect of Trichoderma vermifimicola strain TC467 was evaluated by dual confrontation culture,cellophane and two-compartment culture,pot experiments,and resistance to chemical fungicides.The results demonstrated that TC467 produced substances essential to phytopathogen control(including siderophore,xylanase and chitinase)and plant growth promoters(producing indole-3-acetic acid and gibberellin).The strain displayed a high inhibition rate against Botrytis cinerea reaching 85.26%;and its non-volatile and volatile secondary metabolites showed the inhibition rates to Sclerotinia sclerotiorum and B.cinerea as high as 84.67%and 47.62%,respectively.In pot experiments,comparing with untreated plants TC467 significantly enhanced the height and fresh weight of lettuce(Lactuca sativa var.ramosa)by 46.69%and 15.33%,respectively.Its fermentation broth effectively minimized the lettuce disease caused by B.cinerea with inhibition rate of 87.76%.In addition,the strain showed higher tolerance to hymexazol water-dispersible granule than that to other tested fungicides;at the concentration of 0.42 mg/L the growth rate of TC467 can even approach 98.19%.T.vermifimicola strain TC467 has the potential for practical application in biocontrol especially plant diseases caused by B.cinerea,which extends our knowledge of nature beneficial resources. 展开更多
关键词 biological control trichoderma Botrytis cinerea growth inhibition rate plant diseases
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结直肠癌中SPP1+巨噬细胞的分布和功能及临床意义研究进展
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作者 龙雨欣 杨岳 +1 位作者 金硕(综述) 张红梅(审校) 《中国肿瘤临床》 北大核心 2025年第12期633-637,共5页
结直肠癌(colorectal cancer,CRC)是中国常见的消化道恶性肿瘤之一,其恶性进展与肿瘤微环境(tumor microenvironment,TME)的调控密切相关。肿瘤相关巨噬细胞作为TME中关键的免疫调节细胞,具有显著的异质性特征。分泌型磷蛋白1阳性肿瘤... 结直肠癌(colorectal cancer,CRC)是中国常见的消化道恶性肿瘤之一,其恶性进展与肿瘤微环境(tumor microenvironment,TME)的调控密切相关。肿瘤相关巨噬细胞作为TME中关键的免疫调节细胞,具有显著的异质性特征。分泌型磷蛋白1阳性肿瘤相关巨噬细胞(secreted phosphoprotein 1 tumor-associated macrophages,SPP1+TAM)是一类具有明确促瘤作用的亚群。SPP1+TAM在CRC组织中呈现特异性分布特征,尤其在肿瘤侵袭前沿和转移灶中显著富集。该亚群通过分泌SPP1等效应分子,参与结肠癌细胞黏附、迁移、血管生成和转移等多种活动。本文就SPP1+TAM在CRC中的分布特征、分子调控机制及其临床意义进行综述,旨在为开发新型诊断标志物和靶向治疗策略提供理论依据。 展开更多
关键词 结直肠癌 肿瘤微环境 spp1+巨噬细胞 骨桥蛋白
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Trichoderma aureoviride hyphal pellets embedded in corncob-sodium alginate matrix for efficient uranium(VI)biosorption from aqueous solutions
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作者 Min Li Bo Liang +3 位作者 Jie-ming Liu Jin Zhang Bin Wang Jie Shang 《Water Science and Engineering》 2025年第1期51-58,共8页
The discharge of effluents containing uranium(U)ions into aquatic ecosystems poses significant risks to both human health and marine organisms.This study investigated the biosorption of U(VI)ions from aqueous solution... The discharge of effluents containing uranium(U)ions into aquatic ecosystems poses significant risks to both human health and marine organisms.This study investigated the biosorption of U(VI)ions from aqueous solutions using corncob-sodium alginate(SA)-immobilized Trichoderma aureoviride hyphal pellets.Experimental parameters,including initial solution pH,initial concentration,temperature,and contact time,were systematically examined to understand their influence on the bioadsorption process.Results showed that the corncob-SA-immobilized T.aureoviride hyphal pellets exhibited maximum uranium biosorption capacity at an initial pH of 6.23 and a contact time of 12 h.The equilibrium data aligned with the Langmuir isotherm model,with a maximum biosorption capacity of 105.60 mg/g at 301 K.Moreover,biosorption kinetics followed the pseudo-second-order kinetic model.In terms of thermodynamic parameters,the changes in Gibbs-free energy(△G°)were determined to be-4.29 kJ/mol at 301 K,the changes in enthalpy(△H°)were 46.88 kJ/mol,and the changes in entropy(△S°)was 164.98 J/(mol·K).Notably,the adsorbed U(VI)could be efficiently desorbed using Na_(2)CO_(3),with a maximum readsorption efficiency of 53.6%.Scanning electron microscopic(SEM)analysis revealed U(VI)ion binding onto the hyphal pellet surface.This study underscores the efficacy of corncob-SA-immobilized T.aureoviride hyphal pellets as a cost-effective and environmentally favorable biosorbent material for removing U(VI)from aquatic ecosystems. 展开更多
关键词 BIOSORPTION URANIUM trichoderma aureoviride Immobilization CORNCOB
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