Two novel koninginin derivatives,koningipyridines A and B(1 and 2),along with four known compounds(3-6)were isolated from the EtOAc extract of the endophytic fungus Trichoderma koningiopsis SC-5.Among them,koningipyri...Two novel koninginin derivatives,koningipyridines A and B(1 and 2),along with four known compounds(3-6)were isolated from the EtOAc extract of the endophytic fungus Trichoderma koningiopsis SC-5.Among them,koningipyridine A featured an unprecedented pentacyclic ketal skeleton with the formation of a fascinating 6/6/5/6/5 fused ring system and shared a characteristic pyridine core,which represents the first example of nitrogen-containing koninginin-type natural product.Moreover,koningipyridine B was the first member in the koninginin family sharing a unique 6/6/5 dihydropyridine skeleton,and it was suggested to be the critical biosynthetic precursor of koningipyridine A.The structures of 1 and 2 were elucidated by the interpretation of 1D and 2D NMR spectroscopy,HRESIMS data,as well as theoretical calculations of 13C NMR and electronic circular dichroism(ECD).Moreover,all isolates were screened for antimicrobial activities against Staphylococcus aureus,MRSA,and Escherichia coli as well as the cytotoxic effects against three cancer cell lines(A549,Hela,and HepG2).展开更多
Four new fungal polyketides named koninginins N-Q(1–4),together with four known analogues(5–8),were isolated from the endophytic fungus Trichoderma koningiopsis YIM PH30002 harbored in Panax notoginseng.Their struct...Four new fungal polyketides named koninginins N-Q(1–4),together with four known analogues(5–8),were isolated from the endophytic fungus Trichoderma koningiopsis YIM PH30002 harbored in Panax notoginseng.Their structures were determined on the basis of spectral data interpretation.These compounds were evaluated for their antifungal activity,nitric oxide inhibition,and anticoagulant activity.展开更多
基金Financial support for this research was provided by the National Natural Science Foundation of China(Nos.82173711 and 82003929)Youth Innovation Promotion Association of CAS(2020342)+2 种基金Natural Science Foundation of Hunan Province(Nos.2021JJ30917 and 2021JJ40993)Key Research and Development Project of Hainan Province(No.ZDYF2022SHFZ048)the Central South University postgraduate independent exploration and innovation project(No.2022zzts0899).
文摘Two novel koninginin derivatives,koningipyridines A and B(1 and 2),along with four known compounds(3-6)were isolated from the EtOAc extract of the endophytic fungus Trichoderma koningiopsis SC-5.Among them,koningipyridine A featured an unprecedented pentacyclic ketal skeleton with the formation of a fascinating 6/6/5/6/5 fused ring system and shared a characteristic pyridine core,which represents the first example of nitrogen-containing koninginin-type natural product.Moreover,koningipyridine B was the first member in the koninginin family sharing a unique 6/6/5 dihydropyridine skeleton,and it was suggested to be the critical biosynthetic precursor of koningipyridine A.The structures of 1 and 2 were elucidated by the interpretation of 1D and 2D NMR spectroscopy,HRESIMS data,as well as theoretical calculations of 13C NMR and electronic circular dichroism(ECD).Moreover,all isolates were screened for antimicrobial activities against Staphylococcus aureus,MRSA,and Escherichia coli as well as the cytotoxic effects against three cancer cell lines(A549,Hela,and HepG2).
基金This work was supported partly by grants from the National Natural Science Foundation of China(Nos.81360480,21262041 and 81460536).
文摘Four new fungal polyketides named koninginins N-Q(1–4),together with four known analogues(5–8),were isolated from the endophytic fungus Trichoderma koningiopsis YIM PH30002 harbored in Panax notoginseng.Their structures were determined on the basis of spectral data interpretation.These compounds were evaluated for their antifungal activity,nitric oxide inhibition,and anticoagulant activity.
