Amphenicol residues in foods of animal origin can pose severe risks to human health;however,the determination of amphenicols is usually based on a single-mode immunoassays.So a multi-mode method capable of determining...Amphenicol residues in foods of animal origin can pose severe risks to human health;however,the determination of amphenicols is usually based on a single-mode immunoassays.So a multi-mode method capable of determining amphenicols is desirable.In this study,a tri-mode fluorescence detection method for amphenicols was developed on the basis of the ribosomal protein L16(rpL16)of Thermus thermophilus.In particular,rpL16 was expressed,and its recognition mechanism for three amphenicols was investigated using a molecular docking technique.The protein was conjugated to red quantum dots to prepare a recognition element,which was combined with a blue carbon dotlabeled fluorescent tracer to develop a direct competitive microplate-based assay for the detection of amphenicols.As the analyte concentration increased,there was a color change from purple to red,the RGB value increased,and the fluorescence intensity from the tracer decreased.The results could be read with the naked eye,a smartphone,and a multimode microplate reader.The limits of detection for determination of the three amphenicols in eggs were 1.0 ng/g via visual detection,10 pg/g via smartphone detection,and 0.05-0.17 pg/g via instrumental detection.This study reports the first tri-mode detection method for the determination of small molecule compounds based on quantum dots and carbon dots,providing a versatile tool for amphenicol detection in foods of animal origin under different usage scenarios.展开更多
Interfacial fluid mixing driven by a shock wave is a common phenomenon that occurs frequently in basic science research and in a variety of applications.In this work,shock-tube experiments on the developments of two-d...Interfacial fluid mixing driven by a shock wave is a common phenomenon that occurs frequently in basic science research and in a variety of applications.In this work,shock-tube experiments on the developments of two-dimensional tri-mode interfaces accelerated by a convergent shock wave are performed.Eight sets of different combinations in the value of phase and that of amplitude in a tri-mode perturbed interface are studied to evaluate the effect of mode coupling on the amplitude growths of the basic modes.The qualitative results show that the phase combination obviously affects the interface morphologies and flow features.The alternation of each mode amplitude does not affect the major flow features,such as the number and arrangement of the bubbles and spikes,but affects the local interface features.Depending upon the phase combination,the mode amplitude growth is either promoted or suppressed by mode coupling relative to the single-mode counterpart.By considering the feedbacks from both the first-order and second-order mode couplings,the mode amplitude growth can be qualitatively predicted.Relative to dual-mode interface,mode coupling occurs earlier in tri-mode interface.For the sets of parameters we studied,the effect of initial phase on the amplitude growth is greater and occurs earlier for the mode with low mode number.In addition,the amplitude development of mode with high mode number is more affected by initial amplitudes of basic modes than that of mode with low mode number.The introduction of the third mode affects the amplitude growths of original two modes,but has little effect on the final mixing width growth.Finally,a theoretical model is proposed to predict the amplitude growth of each basic mode.展开更多
To control the tri-modal microstructure and performance,a prediction model of tri-modal microstructure in the isothermal local loading forming of titanium alloy was developed.The staged isothermal local loading experi...To control the tri-modal microstructure and performance,a prediction model of tri-modal microstructure in the isothermal local loading forming of titanium alloy was developed.The staged isothermal local loading experiment on TA15alloy indicates that there exist four important microstructure evolution phenomena in the development of tri-modal microstructure,i.e.,the generation of lamellarα,content variation of equiaxedα,spatial orientation change of lamellarαand globularization of lamellarα.Considering the laws of these microstructure phenomena,the microstructure model was established to correlate the parameters of tri-modal microstructure and processing conditions.Then,the developed microstructure model was integrated with finite element(FE)model to predict the tri-modal microstructure in the isothermal local loading forming.Its reliability and accuracy were verified by the microstructure observation at different locations of sample.Good agreements between the predicted and experimental results suggest that the developed microstructure model and its combination with FE model are effective in the prediction of tri-modal microstructure in the isothermal local loading forming of TA15alloy.展开更多
Although declining in the US due to restrictions of asbestos exposure, malignant pleura/mesothelioma （MPP） remains a very serious thoracic malignancy that is rising in incidence worldwide （1）. Trirnodality therapy...Although declining in the US due to restrictions of asbestos exposure, malignant pleura/mesothelioma （MPP） remains a very serious thoracic malignancy that is rising in incidence worldwide （1）. Trirnodality therapy with chemotherapy and radiotherapy combined with extrapleural pneumonectomy （EPP） has gained acceptance given the acceptable mortality rate （〈5%） and long term survival reported in patients with epithelial histology, negative margins, and no extrapleural lymph node involvement after trimodalitv treatment （2）.展开更多
基金support of Science and Technology Program of Hebei Administration for Market Regulation(No.2024ZC01).
文摘Amphenicol residues in foods of animal origin can pose severe risks to human health;however,the determination of amphenicols is usually based on a single-mode immunoassays.So a multi-mode method capable of determining amphenicols is desirable.In this study,a tri-mode fluorescence detection method for amphenicols was developed on the basis of the ribosomal protein L16(rpL16)of Thermus thermophilus.In particular,rpL16 was expressed,and its recognition mechanism for three amphenicols was investigated using a molecular docking technique.The protein was conjugated to red quantum dots to prepare a recognition element,which was combined with a blue carbon dotlabeled fluorescent tracer to develop a direct competitive microplate-based assay for the detection of amphenicols.As the analyte concentration increased,there was a color change from purple to red,the RGB value increased,and the fluorescence intensity from the tracer decreased.The results could be read with the naked eye,a smartphone,and a multimode microplate reader.The limits of detection for determination of the three amphenicols in eggs were 1.0 ng/g via visual detection,10 pg/g via smartphone detection,and 0.05-0.17 pg/g via instrumental detection.This study reports the first tri-mode detection method for the determination of small molecule compounds based on quantum dots and carbon dots,providing a versatile tool for amphenicol detection in foods of animal origin under different usage scenarios.
基金supported by the National Natural Science Foundation of China (Grant Nos.12372281 and 12388101)the Strategic Priority Research Program of Chinese Academy of Sciences (Grant No.XDB0620201)Youth Innovation Promotion Association CAS。
文摘Interfacial fluid mixing driven by a shock wave is a common phenomenon that occurs frequently in basic science research and in a variety of applications.In this work,shock-tube experiments on the developments of two-dimensional tri-mode interfaces accelerated by a convergent shock wave are performed.Eight sets of different combinations in the value of phase and that of amplitude in a tri-mode perturbed interface are studied to evaluate the effect of mode coupling on the amplitude growths of the basic modes.The qualitative results show that the phase combination obviously affects the interface morphologies and flow features.The alternation of each mode amplitude does not affect the major flow features,such as the number and arrangement of the bubbles and spikes,but affects the local interface features.Depending upon the phase combination,the mode amplitude growth is either promoted or suppressed by mode coupling relative to the single-mode counterpart.By considering the feedbacks from both the first-order and second-order mode couplings,the mode amplitude growth can be qualitatively predicted.Relative to dual-mode interface,mode coupling occurs earlier in tri-mode interface.For the sets of parameters we studied,the effect of initial phase on the amplitude growth is greater and occurs earlier for the mode with low mode number.In addition,the amplitude development of mode with high mode number is more affected by initial amplitudes of basic modes than that of mode with low mode number.The introduction of the third mode affects the amplitude growths of original two modes,but has little effect on the final mixing width growth.Finally,a theoretical model is proposed to predict the amplitude growth of each basic mode.
基金Projects(51605388,51575449)supported by the National Natural Science Foundation of ChinaProject(B08040)supported by the "111" Project,China+1 种基金Project(131-QP-2015)supported by the Research Fund of the State Key Laboratory of Solidification Processing(NWPU),ChinaProject supported by the Open Research Fund of State Key Laboratory of Materials Processing and Die&Mould Technology,Huazhong University of Science and Technology,China
文摘To control the tri-modal microstructure and performance,a prediction model of tri-modal microstructure in the isothermal local loading forming of titanium alloy was developed.The staged isothermal local loading experiment on TA15alloy indicates that there exist four important microstructure evolution phenomena in the development of tri-modal microstructure,i.e.,the generation of lamellarα,content variation of equiaxedα,spatial orientation change of lamellarαand globularization of lamellarα.Considering the laws of these microstructure phenomena,the microstructure model was established to correlate the parameters of tri-modal microstructure and processing conditions.Then,the developed microstructure model was integrated with finite element(FE)model to predict the tri-modal microstructure in the isothermal local loading forming.Its reliability and accuracy were verified by the microstructure observation at different locations of sample.Good agreements between the predicted and experimental results suggest that the developed microstructure model and its combination with FE model are effective in the prediction of tri-modal microstructure in the isothermal local loading forming of TA15alloy.
文摘Although declining in the US due to restrictions of asbestos exposure, malignant pleura/mesothelioma （MPP） remains a very serious thoracic malignancy that is rising in incidence worldwide （1）. Trirnodality therapy with chemotherapy and radiotherapy combined with extrapleural pneumonectomy （EPP） has gained acceptance given the acceptable mortality rate （〈5%） and long term survival reported in patients with epithelial histology, negative margins, and no extrapleural lymph node involvement after trimodalitv treatment （2）.
