目的:观察弱内向整流相关及花生四烯酸激活的弱内向整流相关双孔钾离子通道(tandem of pore domains in a weak inward rectifying related-tandem of pore domains in a weak inward rectifying related arachidonic acid activated tw...目的:观察弱内向整流相关及花生四烯酸激活的弱内向整流相关双孔钾离子通道(tandem of pore domains in a weak inward rectifying related-tandem of pore domains in a weak inward rectifying related arachidonic acid activated two pore-domain potassium channels, TREK-TRAAK K2P)激活对氧化损伤的人视网膜色素上皮(retinal pigment epithelial, RPE)细胞吞噬功能的影响。方法:免疫荧光法检测TREK-1、TREK-2及TRAAK通道蛋白在人RPE细胞的表达,以不同时间和浓度梯度的叔丁基过氧化氢(tertbutyl hydroperoxide, t-BHP)诱导人RPE细胞氧化损伤。分对照组、t-BHP组、利鲁唑加t-BHP组,利鲁唑组,向各组加入2×107/mL的荧光微球或FITC标记猪感光细胞外节膜盘孵育6 h,固定染色后荧光显微镜拍照,Image-Pro Plus 6.0软件分析计算RPE细胞吞噬率和吞噬指数。结果:TREK-1、TREK-2、TREEK通道蛋白亚型在人RPE细胞质中均高表达。人RPE细胞在t-BHP干预后存活率呈浓度和时间依赖性,200μmol/L t-BHP干预6 h与对照组存活率比较差异无统计学意义(P>0.05),400μmol/L t-BHP干预6 h致半数死亡。特异性吞噬指数:t-BHP组低于其他各组(P<0.001),而利鲁唑组高于对照组,差异无统计学意义(P>0.05)。特异性吞噬率:t-BHP组低于其他各组,差异无统计学意义(P>0.05)。非特异性吞噬指数:t-BHP组低于其他各组(P<0.001);非特异性吞噬率:t-BHP组低于利鲁唑加t-BHP组,差异无统计学意义(P>0.05),两两比较,仅利鲁唑组高于t-BHP组(P<0.05)。结论:激活TREK-TRAAK K2P通道可保护氧化损伤人RPE细胞的吞噬功能。展开更多
On 22 January 2025,the delegation of Harvard Students'China Trek 2025 arrived in Hangzhou.Forty-six students from Harvard Kennedy School,representing 20 countries,engaged in in-depth exchanges with Chinese and int...On 22 January 2025,the delegation of Harvard Students'China Trek 2025 arrived in Hangzhou.Forty-six students from Harvard Kennedy School,representing 20 countries,engaged in in-depth exchanges with Chinese and international youth delegates,embarking on a journey promoting mutual learning and strengthening friendship.展开更多
文摘目的:观察弱内向整流相关及花生四烯酸激活的弱内向整流相关双孔钾离子通道(tandem of pore domains in a weak inward rectifying related-tandem of pore domains in a weak inward rectifying related arachidonic acid activated two pore-domain potassium channels, TREK-TRAAK K2P)激活对氧化损伤的人视网膜色素上皮(retinal pigment epithelial, RPE)细胞吞噬功能的影响。方法:免疫荧光法检测TREK-1、TREK-2及TRAAK通道蛋白在人RPE细胞的表达,以不同时间和浓度梯度的叔丁基过氧化氢(tertbutyl hydroperoxide, t-BHP)诱导人RPE细胞氧化损伤。分对照组、t-BHP组、利鲁唑加t-BHP组,利鲁唑组,向各组加入2×107/mL的荧光微球或FITC标记猪感光细胞外节膜盘孵育6 h,固定染色后荧光显微镜拍照,Image-Pro Plus 6.0软件分析计算RPE细胞吞噬率和吞噬指数。结果:TREK-1、TREK-2、TREEK通道蛋白亚型在人RPE细胞质中均高表达。人RPE细胞在t-BHP干预后存活率呈浓度和时间依赖性,200μmol/L t-BHP干预6 h与对照组存活率比较差异无统计学意义(P>0.05),400μmol/L t-BHP干预6 h致半数死亡。特异性吞噬指数:t-BHP组低于其他各组(P<0.001),而利鲁唑组高于对照组,差异无统计学意义(P>0.05)。特异性吞噬率:t-BHP组低于其他各组,差异无统计学意义(P>0.05)。非特异性吞噬指数:t-BHP组低于其他各组(P<0.001);非特异性吞噬率:t-BHP组低于利鲁唑加t-BHP组,差异无统计学意义(P>0.05),两两比较,仅利鲁唑组高于t-BHP组(P<0.05)。结论:激活TREK-TRAAK K2P通道可保护氧化损伤人RPE细胞的吞噬功能。
文摘On 22 January 2025,the delegation of Harvard Students'China Trek 2025 arrived in Hangzhou.Forty-six students from Harvard Kennedy School,representing 20 countries,engaged in in-depth exchanges with Chinese and international youth delegates,embarking on a journey promoting mutual learning and strengthening friendship.
