[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌...[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。展开更多
Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is...Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is widely used in various applications.Its properties and applications are closely tied to its tacticity.One-dimensional(1D) conventional 13C NMR has been extensively utilized to analyze PP tacticity,but its low sensitivity and longer relaxation time remain drawbacks.Typically,analyzing a single PP sample requires around 9 h of NMR time.Using of a cryogenically cooled 10 mm NMR probe can significantly reduce this measurement time;however,its high cost makes it inaccessible for most NMR laboratories.While the refocused insensitive nuclei enhanced by polarization transfer(RINEPT) technique is well-known for enhancing NMR sensitivity,there are no published studies using 1D 13C RINEPT to quantify PP tacticity.Relaxation agents like chromium(Ⅲ) acetylacetonate(Cr(acac)_(3)) have also been used to reduce relaxation times in polyolefin NMR analyses.Here we introduce a straightforward and easily implementable 1D 13C NMR method for rapid PP tacticity quantification.This method combines Cr(acac)_(3),Bruker's existing RINEPT pulse sequence(ineptrd),and our recently published ~1H decoupling sequence(bi_waltz65_256 pl) to eliminate ^(1)H decoupling artifacts.It is worth noting that decoupling artifacts are always present.When the signal-to-noise ratio(SNR) is low,these artifacts are obscured by noise.For example,in some two-dimensional(2D) or three-dimensional(3D) NMR spectra,decoupling artifacts are barely visible because of the low SNR.However,when attempting to observe weak signals in ^(1)D spectra,increasing the sample concentration or the number of scans enhances the SNR,revealing the decoupling artifacts.The decoupling artifacts appeared superimpose with some other weak signals,affecting the measurements of signal intensities.Therefore,improved ~1H-decoupling methods are crucial for such data acquisitio n.This synergy results in a 9.4-to 9.7-fold sensitivity enhancement,equating to an 88-to 94-fold reduction(9.4^(2)≈88,9.7^(2)≈94) in NMR acquisition time compared to conventional 1D ^(13)C NMR experiment with Cr(acac)_(3).The time savings are even more substantial compared to experiments without Cr(acac)_(3).The faster and quantitative approach is accessible to researchers with or without cryoprobes.Beyond PP,this method can be applied to tacticity measurements of other polyolefins,such as polybutene,polyhexene and polyoctene.展开更多
Straw return has demonstrated significant potential for enhancing carbon(C)sequestration and nitrogen(N)uptake while concurrently promoting plant productivity.However,the specific transport and distribution of C produ...Straw return has demonstrated significant potential for enhancing carbon(C)sequestration and nitrogen(N)uptake while concurrently promoting plant productivity.However,the specific transport and distribution of C produced by photosynthesis and exogenous N within the rice plant-soil system under straw return remains unclear.A long-term straw return pot trial experiment was conducted in a double cropping rice system,incorporating treatments of inorganic fertilizer application with straw removal(F),straw burning and ash return with reducing inorganic fertilizers(SBR),and straw return with reducing inorganic fertilizers(SR)to investigate C sequestration and exogenous N uptake using ^(13)C pulse and ^(15)N isotope tracer techniques.The SR treatment had significantly higher soil ^(13)C abundance,by 24.4 and 25.4%,respectively,^(13)C concentrations in aboveground plant parts,by 18.4 and 35.8%respectively,and ^(15)N concentrations in rice panicles,by 12.8 and 34.3%than the SBR and F treatments.This enhancement contributed to a higher total organic C concentration and increased rice grain yield in the SR treatment.Furthermore,the SR treatment had significantly higher photosynthetic C,by 9.8%,which was directly transferred to soil C.The SR treatment had a higher distribution of photosynthetic C in the leaves and stems,but a lower distribution in the panicle compared to the SBR treatment.This finding is advantageous for sequestering photosynthetic C into the soil through straw return;conversely,opposite trends were observed in ^(15)N distribution.In addition,rice plants in the SR treatment had increased N uptake from urea and soil N sources,enhancing N recovery by 9.2 and 12.5%,respectively,and reducing soil N residues.Correlation analysis showed that the SR treatment increased the concentrations of ^(13)C in leaves and roots while decreasing the ^(15)N abundance in all rice organs,thereby contributing to an increase in rice yield.The partial least square path model suggested that the increase in rice yield under the SR treatment was primarily linked to ^(13)C accumulation within the rice plant-soil system.The results suggest that straw return increases the sequestration of photosynthetic C and exogenous N in the rice plant-soil system and increases N utilization efficiency,which subsequently improves both rice and soil productivity.展开更多
文摘[目的]探究OR13C2在宫颈癌组织中的表达以及对宫颈癌恶性转移的影响。[方法]通过蛋白免疫印迹和实时的荧光定量PCR检测宫颈癌组织OR13C2表达情况。将宫颈癌细胞分为pcDNA NC组、pcDNA OR13C2组和抑制剂(PI-103)组。CCK-8实验分析宫颈癌细胞增殖能力,Transwell实验和划痕实验分析宫颈癌细胞侵袭和迁移能力,流式细胞术检测宫颈癌细胞的凋亡率,蛋白免疫印迹分析OR13C2对PI3K/AKT通路蛋白表达的影响。[结果]OR13C2在宫颈癌组织中低表达,而在宫颈癌旁组织中高表达(0.23±0.02 vs 0.97±0.07;2.01±0.05 vs 4.81±0.13;P<0.05)。OR13C2高表达后能够抑制宫颈癌细胞的增殖、侵袭和迁移能力,促进宫颈癌细胞凋亡。此外,OR13C2高表达能够抑制PI3K/AKT的激活(0.73±0.03 vs 0.19±0.05 vs 0.26±0.08;0.72±0.08 vs 0.23±0.06 vs 0.31±0.03;P<0.05)。[结论]OR13C2在宫颈癌组织中表达减少,上调OR13C2的表达可以抑制宫颈癌HeLa细胞的增殖、侵袭和迁移能力,促进宫颈癌HeLa细胞凋亡,此作用与OR13C2抑制PI3K/AKT通路的激活相关。
文摘Polypropylene(PP) accounts for approximately 28.0% of the global polyolefin market,valued at $243.4 billion in 2022.Known for its lightweight,chemical resistance,costeffectiveness,high strength and melting point,PP is widely used in various applications.Its properties and applications are closely tied to its tacticity.One-dimensional(1D) conventional 13C NMR has been extensively utilized to analyze PP tacticity,but its low sensitivity and longer relaxation time remain drawbacks.Typically,analyzing a single PP sample requires around 9 h of NMR time.Using of a cryogenically cooled 10 mm NMR probe can significantly reduce this measurement time;however,its high cost makes it inaccessible for most NMR laboratories.While the refocused insensitive nuclei enhanced by polarization transfer(RINEPT) technique is well-known for enhancing NMR sensitivity,there are no published studies using 1D 13C RINEPT to quantify PP tacticity.Relaxation agents like chromium(Ⅲ) acetylacetonate(Cr(acac)_(3)) have also been used to reduce relaxation times in polyolefin NMR analyses.Here we introduce a straightforward and easily implementable 1D 13C NMR method for rapid PP tacticity quantification.This method combines Cr(acac)_(3),Bruker's existing RINEPT pulse sequence(ineptrd),and our recently published ~1H decoupling sequence(bi_waltz65_256 pl) to eliminate ^(1)H decoupling artifacts.It is worth noting that decoupling artifacts are always present.When the signal-to-noise ratio(SNR) is low,these artifacts are obscured by noise.For example,in some two-dimensional(2D) or three-dimensional(3D) NMR spectra,decoupling artifacts are barely visible because of the low SNR.However,when attempting to observe weak signals in ^(1)D spectra,increasing the sample concentration or the number of scans enhances the SNR,revealing the decoupling artifacts.The decoupling artifacts appeared superimpose with some other weak signals,affecting the measurements of signal intensities.Therefore,improved ~1H-decoupling methods are crucial for such data acquisitio n.This synergy results in a 9.4-to 9.7-fold sensitivity enhancement,equating to an 88-to 94-fold reduction(9.4^(2)≈88,9.7^(2)≈94) in NMR acquisition time compared to conventional 1D ^(13)C NMR experiment with Cr(acac)_(3).The time savings are even more substantial compared to experiments without Cr(acac)_(3).The faster and quantitative approach is accessible to researchers with or without cryoprobes.Beyond PP,this method can be applied to tacticity measurements of other polyolefins,such as polybutene,polyhexene and polyoctene.
基金supported by the National Natural Science Foundation of China(32160503)the Earmarked Fund for Jiangxi Agriculture Research System,China(JXARS-01)the National Key R&D Program of China(2023YFD2301303).
文摘Straw return has demonstrated significant potential for enhancing carbon(C)sequestration and nitrogen(N)uptake while concurrently promoting plant productivity.However,the specific transport and distribution of C produced by photosynthesis and exogenous N within the rice plant-soil system under straw return remains unclear.A long-term straw return pot trial experiment was conducted in a double cropping rice system,incorporating treatments of inorganic fertilizer application with straw removal(F),straw burning and ash return with reducing inorganic fertilizers(SBR),and straw return with reducing inorganic fertilizers(SR)to investigate C sequestration and exogenous N uptake using ^(13)C pulse and ^(15)N isotope tracer techniques.The SR treatment had significantly higher soil ^(13)C abundance,by 24.4 and 25.4%,respectively,^(13)C concentrations in aboveground plant parts,by 18.4 and 35.8%respectively,and ^(15)N concentrations in rice panicles,by 12.8 and 34.3%than the SBR and F treatments.This enhancement contributed to a higher total organic C concentration and increased rice grain yield in the SR treatment.Furthermore,the SR treatment had significantly higher photosynthetic C,by 9.8%,which was directly transferred to soil C.The SR treatment had a higher distribution of photosynthetic C in the leaves and stems,but a lower distribution in the panicle compared to the SBR treatment.This finding is advantageous for sequestering photosynthetic C into the soil through straw return;conversely,opposite trends were observed in ^(15)N distribution.In addition,rice plants in the SR treatment had increased N uptake from urea and soil N sources,enhancing N recovery by 9.2 and 12.5%,respectively,and reducing soil N residues.Correlation analysis showed that the SR treatment increased the concentrations of ^(13)C in leaves and roots while decreasing the ^(15)N abundance in all rice organs,thereby contributing to an increase in rice yield.The partial least square path model suggested that the increase in rice yield under the SR treatment was primarily linked to ^(13)C accumulation within the rice plant-soil system.The results suggest that straw return increases the sequestration of photosynthetic C and exogenous N in the rice plant-soil system and increases N utilization efficiency,which subsequently improves both rice and soil productivity.
