To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified th...To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.展开更多
A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, caul...A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root respectively. Furthermore, this expression mode was highly correlated with AsA levels.展开更多
This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced u...This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.展开更多
With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this c...With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this context,Rosa roxburghii Tratt(RR)has emerged as a potential source of skin-lightening compounds.Vitamin C(Vc)and superoxide dismutase(SOD)-rich extracts were extracted from RR,namely RRVC and RRSOD,using macroporous resin column chromatography and graded ammonium sulfate precipitation.The antioxidant and skin-lightening effects of these extracts were evaluated.RRVC and RRSOD showed strong inhibitory activity against various radicals and tyrosinase.When formulated together,their antioxidant and skin-lightening abilities were significantly enhanced,with the optimal ratio of RRVC and RRSOD(RRVS)of 5:32.In vivo studies on C57BL/6J mice revealed that these two extracts and their formulations effectively lowered oxidative stress levels in a model of d-galactose-induced aging in mice.Furthermore,they inhibited melanogenesis and tyrosinase activity in B16 cells and reduced ultravioletradiation-induced melanin deposition in mouse ears.The combination of RRVC and RRSOD had a more potent melanin-scavenging effect compared with the individual extracts.These findings suggest that RRVC and RRSOD,especially when combined at the optimal ratio,can whiten skin,increasing the value of RR products and offering natural alternatives for skin lightening.展开更多
基金supported by the Science and Technology Support Plan of Guizhou Province(Guizhou Family Combination Support 2022,No.116).
文摘To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.
基金supported by the Natural Science Foundation of Guizhou Province of China(20033019 and 20043025).
文摘A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root respectively. Furthermore, this expression mode was highly correlated with AsA levels.
基金supported by Rosa roxburghii industry development program of Guizhou Province,China(QCN2019-261)the National Natural Science Foundation of China(31260379)the National Natural Science Foundation of China(31960485).
文摘This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.
基金supported by the Qiannan Prefecture Science and Technology Support Grant Project(Qiannankehe Support[2024]No.16)the Guizhou Provincial Science and Technology Support Grant Project(Qiankehe Support[2023]No.472 and[2020]No.1Y148)the Guizhou Province Science and Technology Program Funding Project(Qian Kehe Basic-2K[2022]No.445),China.
文摘With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this context,Rosa roxburghii Tratt(RR)has emerged as a potential source of skin-lightening compounds.Vitamin C(Vc)and superoxide dismutase(SOD)-rich extracts were extracted from RR,namely RRVC and RRSOD,using macroporous resin column chromatography and graded ammonium sulfate precipitation.The antioxidant and skin-lightening effects of these extracts were evaluated.RRVC and RRSOD showed strong inhibitory activity against various radicals and tyrosinase.When formulated together,their antioxidant and skin-lightening abilities were significantly enhanced,with the optimal ratio of RRVC and RRSOD(RRVS)of 5:32.In vivo studies on C57BL/6J mice revealed that these two extracts and their formulations effectively lowered oxidative stress levels in a model of d-galactose-induced aging in mice.Furthermore,they inhibited melanogenesis and tyrosinase activity in B16 cells and reduced ultravioletradiation-induced melanin deposition in mouse ears.The combination of RRVC and RRSOD had a more potent melanin-scavenging effect compared with the individual extracts.These findings suggest that RRVC and RRSOD,especially when combined at the optimal ratio,can whiten skin,increasing the value of RR products and offering natural alternatives for skin lightening.
