To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified th...To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.展开更多
A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, caul...A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root respectively. Furthermore, this expression mode was highly correlated with AsA levels.展开更多
This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced u...This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.展开更多
Lactobacillus acidophilus GIM1.208(LA)can improve flavor,enhance antioxidant and hypoglycemic activities of Rosa roxburghii Tratt(RRT).This study investigated the interactions between proteins and active compounds in ...Lactobacillus acidophilus GIM1.208(LA)can improve flavor,enhance antioxidant and hypoglycemic activities of Rosa roxburghii Tratt(RRT).This study investigated the interactions between proteins and active compounds in RRT fermented by LA to reveal the underlying reasons for changes in flavor and activity.A total of 6239 proteins were characterized and quantified by Label-free proteomics,with their molecular weights were mainly concentrated in the range of 10-40 kDa.The 129 differential proteins screened were mainly enriched in compounds synthesis or transformation pathways,such as glycolysis.Integrated analysis of proteomics and metabolomics revealed a strong correlation between proteins and metabolites,especiallyβ-glucosidase was closely correlation with flavonoids and triterpenoids.Additionally,molecular dynamics(MD)simulations have demonstrated that glycosides establish hydrogen bonds with the amino acids ofβ-glucosidase,while hydrophobic interactions serve as the primary driving force behind the binding process.Notably,Rosamutin exhibited the most significant binding energy withβ-glucosidase(66.257 kJ/mol).The results of the MD analysis further demonstrate the huge potential of LAβ-glucosidase to transform glycosides in RRT.In summary,proteins in LA fermented RRT contribute to the consumption or synthesis of compounds,ultimately enhancing the flavor and activity of RRT.This study’s results can provide a reference for the research of proteins and bioactive compounds of RRT.展开更多
Dietary fiber(DF),an indigestible component of plant cell walls,is widely recognized for its health benefits.Rosa roxburghii Tratt(RRT)pomace contains high levels of DF,known for their anti-inflammatory and antioxidan...Dietary fiber(DF),an indigestible component of plant cell walls,is widely recognized for its health benefits.Rosa roxburghii Tratt(RRT)pomace contains high levels of DF,known for their anti-inflammatory and antioxidant properties,which are beneficial in managing atherosclerosis and diabetes;however,knowledge about the DF in RRT is limited.This study aimed to determine whether bound polyphenols(BPs)contribute to the health benefits of RRT’s DF and assess its potential as a valuable source of DF.DF was extracted from RRT(RRT-DF)using an enzymatic method and BPs removed from RRT-DF through alkaline extraction(RRT-DF-F),and the micro-structural,physicochemical,antioxidant,and adsorption properties were compared between RRT-DF and RRTDFF.The results showed no significant alteration in the microstructure,crystalline peaks,or characteristic bonds of the obtained fibers,and RRT-DF showed improved water-and oil-holding capacities.RRT-DF-F demonstrated significantly lower DPPH and OH-scavenging activity than RRT-DF,and the adsorption capacity for nitrite,cholesterol,and bile acid,and theα-glucosidase activity inhibition ratio was significantly decreased.These findings indicate that BPs contribute to the in vitro physicochemical and functional properties of RRT-DF,highlighting its potential as a functional food ingredient and supporting the high-value utilization of RRT byproducts.展开更多
Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this stu...Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this study,the proliposomes containing RSO andβC are referred to as Ps,while the modified formulations are denoted as sodium alginate-chitosanmodified proliposomes(SA-CS-Ps).Materials and methods The physicochemical properties,structural characteristics,thermal stability,microstructure,rheological behavior,storage stability,and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps(CS-Ps).Results The particle size and turbidity of Ps increased with the addition of modification layers,reaching 563.73 nm and0.697,respectively,for SA-CS-Ps.CS and SA formed a protective bilayer through electrostatic interactions,which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress.Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76℃,respectively,compared with that of unmodified Ps.During storage,the retention rates(RRs)of RSO andβC followed the order SA-CS-Ps>CS-Ps>Ps,whereas malondialdehyde and peroxide values exhibited the opposite trend.Elevated temperatures accelerated oxidation,thereby compromising the protective effect of the carriers.After 24 d at 4℃,SA-CSPs demonstrated superior stability,with malondialdehyde of 0.016μg/mg,peroxide value of 2.697 mmol/kg,and RRs of RSO of 97.15%andβC of 96.37%.The oxidation kinetics conformed to a first-order reaction model.Conclusions Given its enhanced protection against degradation and stability,SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds.This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.展开更多
The key polyphenolic compounds in polyphenol extracts from Rosa roxburghii distiller’s grains(R.roxburghii DGs)were crucial in regulating intestinal homeostasis effectively.Thus,an integrative analysis combining meta...The key polyphenolic compounds in polyphenol extracts from Rosa roxburghii distiller’s grains(R.roxburghii DGs)were crucial in regulating intestinal homeostasis effectively.Thus,an integrative analysis combining metagenomics and metabolomics investigated the interactions and mechanisms between the combination of key polyphenols from R.roxburghii DGs(CKPR)and the fecal microbiota.The free polyphenols in CKPR(CKPR-FP)regulated intestinal homeostasis more effectively than the bound polyphenols in CKPR(CKPR-BP)by decreasing the Bacteroidetes/Firmicutes(B/F)ratio and targeted modulating gut microbiota.Gut microbiota metabolized CKPR into small-molecule bioactives,maintaining microecological stability and contributing to intestinal functions.Additionally,hydroxyphenyl propionic acid and phenylacetic acid stimulated microbial enzymatic pathways related to the degradation of polycyclic aromatic hydrocarbons,xylene,and methyl anthranilate.This research reveals the potential of CKPR and fecal microbiota interaction in maintaining host health.It provides a reference for the precise development of functional foods and nutritional supplements.展开更多
Rosa roxburghii Tratt.(Cili)is a species of rose fruit with distinctive characteristics,combining medicinal and edible values.In recent years,it has become the focus of attention in the field of nutrition and health.F...Rosa roxburghii Tratt.(Cili)is a species of rose fruit with distinctive characteristics,combining medicinal and edible values.In recent years,it has become the focus of attention in the field of nutrition and health.From a health perspective,the core advantage of R.roxburghii lies in its high concentration of bioactive substances.Among these substances,pentacyclic triterpenoids are regarded as one of the key substances that contribute to its health-promoting effects.However,the regulatory mechanisms of triterpenoid biosynthesis remain unclear.The present study found that ultraviolet-B(UV-B)radiation increased the total triterpene content of R.roxburghii by 1.49-fold.The key cyclase gene RrOSC4 encodes an oxidosqualene cyclase implicated in the formation ofα/β-amyrin skeletons under UV-B radiation.Co-expression network and promoter analyses identified RrMYB62 as a key regulator of triterpenoid biosynthesis.Furthermore,over-expression of RrMYB62 upregulated the expression of RrOSC4,leading to increased pentacyclic triterpenoids content in‘NC89’tobacco leaves.Results from yeast one-hybrid,dual-luciferase activity and electrophoretic mobility shift assays demonstrated that RrMYB62 directly binds and activates the promoter of RrOSC4 to regulate triterpenoid biosynthesis.Taken together,our findings demonstrate that triterpenoid biosynthesis in R.roxburghii exposed to UV-B radiation is regulated by the RrMYB62-RrOSC4 module.This study revealed the interplay between light signaling and functional phytochemicals,offering a molecular basis for enhancing the fruit quality in the field of food application.展开更多
With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this c...With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this context,Rosa roxburghii Tratt(RR)has emerged as a potential source of skin-lightening compounds.Vitamin C(Vc)and superoxide dismutase(SOD)-rich extracts were extracted from RR,namely RRVC and RRSOD,using macroporous resin column chromatography and graded ammonium sulfate precipitation.The antioxidant and skin-lightening effects of these extracts were evaluated.RRVC and RRSOD showed strong inhibitory activity against various radicals and tyrosinase.When formulated together,their antioxidant and skin-lightening abilities were significantly enhanced,with the optimal ratio of RRVC and RRSOD(RRVS)of 5:32.In vivo studies on C57BL/6J mice revealed that these two extracts and their formulations effectively lowered oxidative stress levels in a model of d-galactose-induced aging in mice.Furthermore,they inhibited melanogenesis and tyrosinase activity in B16 cells and reduced ultravioletradiation-induced melanin deposition in mouse ears.The combination of RRVC and RRSOD had a more potent melanin-scavenging effect compared with the individual extracts.These findings suggest that RRVC and RRSOD,especially when combined at the optimal ratio,can whiten skin,increasing the value of RR products and offering natural alternatives for skin lightening.展开更多
Rosa roxburghii Tratt(RRT)has been widely studied in recent years due to its rich nutritional composition.In this study,the effects of fermentation by Limosilactobacillus fermentum NCU001464(Lm.fermentum NCU001464)on ...Rosa roxburghii Tratt(RRT)has been widely studied in recent years due to its rich nutritional composition.In this study,the effects of fermentation by Limosilactobacillus fermentum NCU001464(Lm.fermentum NCU001464)on the physicochemical properties and biological activity of dried RRT fruit were investigated.The pH and monosaccharide content of RRT decreased after 48 h of fermentation,the total phenolic content(TPC)increased from 15.06 to 21.74μmol GAE/L,the antioxidant capacity increased and xanthine oxidase(XO)inhibition increased from 25.89 to 36.67%.Moreover,the half-maximal inhibitory concentration(IC50)for XO,of the fermented RRT phenol extract was lower than that of the unfermented phenol extract.Fluorescence spectroscopy confirmed the stronger,inhibitory,binding interactions between XO and fermented RRT.The main binding forces between fermented RRT phenol extract and XO appeared to be hydrogen bonds and van der Waals forces.展开更多
Lactobacillus acidophilus GIM1.208(LA)can greatly improve the volatile flavor and sensory scores of Rosa roxburghii Tratt(RRT),while its effects on nonvolatile flavor and active compounds remains uncertain.Therefore,t...Lactobacillus acidophilus GIM1.208(LA)can greatly improve the volatile flavor and sensory scores of Rosa roxburghii Tratt(RRT),while its effects on nonvolatile flavor and active compounds remains uncertain.Therefore,this study used widely targeted metabolomics to comprehensively analyze the effects of LA on flavor and activity of RRT.After being fermented by LA,the total phenolics and flavonoids content of RRT increased,while the total triterpenoids content slightly decreased.The total antioxidant activity rose by 35.23%,and hypoglycemic activity expressed through α-amylase and α-glucosidase inhibitory activities increased by 51.66% and 24.95%,respectively.A total of 1635 metabolites were identified,including amino acids,phenolic acids,flavonoids,and triterpenes,and 205 different metabolites were screened.The analysis of potential taste-related metabolites among differential metabolites revealed a reduction in amino acid content,while the majority of organic acids and sugars exhibited an increase.The electronic-tongue and electronic-nose showed that LA reduces bitterness and astringency while enhancing the aroma of RRT,indicating changes in flavor compounds lead to improved flavor quality.Additionally,LA can hydrolyze glycoside compounds in RRT and release glycosides with higher activity,which contributes to its increased antioxidant and hypoglycemic effects.In summary,LA induced alterations in the flavor compounds and glycosides of RRT,thereby improved its flavor and activity.This study can serve as a reference for future research on the flavor and activity of RRT,as well as for production quality control.展开更多
基金supported by the Science and Technology Support Plan of Guizhou Province(Guizhou Family Combination Support 2022,No.116).
文摘To identify the pathogen responsible for fruit rot disease in Rosa roxburghii Tratt.from Guiding County,Guizhou Province,China,diseased fruit samples were collected.The pathogen was isolated,purified,and identified through morphological,molecular,and pathogenic analyses.Subsequently,its biological characteristicswere evaluated.Furthermore,to determine the agent with the strongest toxicity against the identified pathogen,the antifungal activity of six chemical and biological agents was evaluated through indoor toxicity assays.Finally,Neopestalotiopsis clavispora was identified as the pathogen responsible for fruit rot disease in R.roxburghii Tratt.The diameter of the pathogen grown under different carbon and nitrogen sources,temperatures,and pH values was measured using the crossintersection method.The optimal carbon and nitrogen sources were soluble starch and peptone,respectively.The optimal growth temperature ranged from 25℃ to 30℃,and the optimal growth pH ranged from 4 to 8.The antifungal effects of six agents,including carvacrol 5%aqueous solution and trifloxystrobin–tebuconazole 75%water-dispersible granules,on the mycelial growth rate of N.clavispora were evaluated.All six agents inhibited N.clavispora,with thiophanate–methyl 70%wettable powder showing the strongest antifungal effect and effectively inhibiting mycelial growth even at the lowest concentration.This was followed by difenoconazole–azoxystrobin 48%suspension concentrate,ethylicin 80%emulsifiable concentrate,and trifloxystrobin–tebuconazole 75%WG,with half-maximal effective concentrations of 0.0105,0.0272,and 0.0368 mg/L,respectively.These findings provide a scientific basis for the application of pesticides in the field-based,environmentally friendly control of fruit rot disease in R.roxburghii Tratt.
基金supported by the Natural Science Foundation of Guizhou Province of China(20033019 and 20043025).
文摘A 855 bp cDNA encoding L-galactono-1,4-lactone dehydrogenase (GalLDH) fragment was cloned from fruit of R. roxburghii Tratt by the method of RT-PCR, on the basis of the homologous genes of Arabidopsis thaliana, cauliflower, sweet potato, strawberry, etc. in GenBank. Sequence analysis showed 79-92% identity in nucleotide sequence and 75-87% identity in amino acid sequence to that of strawberry and Arabidopsis thaliana, etc. Northern blot showed that the expression of GalLDH was significantly different in different organs. The transcription level of GalLDH in fruit was significantly higher than that in leaf, stem and root respectively. Furthermore, this expression mode was highly correlated with AsA levels.
基金supported by Rosa roxburghii industry development program of Guizhou Province,China(QCN2019-261)the National Natural Science Foundation of China(31260379)the National Natural Science Foundation of China(31960485).
文摘This study mainly investigated the regulatory effect of Rosa roxburghii Tratt fruit juice fermented by Lacticaseibacillus paracasei SR10-1(LAB-RRTJ)on modulating gut microbiota in dextran sulfate sodium(DSS)-induced ulcerative colitis in mice.Compared to control group,DSS induction decreased body weight of mice,indexes of Shannon,Simpson,Chao1 and Faith_pd,and increased disease activity index(DAI)and levels of interleukin 1β(IL-1β),IL-6,tumor necrosis factorα(TNF-α)and interferon-γ(IFN-γ);And this induction also led to an increase in Proteobacteria,Verrucomicrobia and Actinobacteria at phylum level,harmful bacterial species richness at genus level,and relative richness of S.sciuri,Desulfovibrio C21_c20,R.gnavus and Akkermansia muciniphila at species level,and a decrease in Firmicutes at phylum level and relative richness of B.acidifaciens in mice.LAB-RRTJ increased body weight of mice with DSS induced ulcerative colitis(UC)and indexes of Shannon,Simpson,Chao1 and Faith_pd,reduced DAI and the content of four infl ammatory factors and improved gut microbiota imbalance in DSS induced UC mice.Besides,the number of operational taxonomic units(OTUs)increased,α-diversity andβ-diversity were restored and similar to those in mice in the control group after LAB-RRTJ treatment.Compared with the positive drug treatment group,LAB-RRTJ has a better effect on regulating gut microbiota diversity in colitis mice.Correlation analysis showed that infl ammatory factors were positively correlated with harmful bacteria and negatively correlated with beneficial bacteria which commonly found in some colitis mice.Taken together,our study demonstrated that LAB-RRTJ could alleviate DSS-induced colitis in mice through the modulation of infl ammatory cytokines and gut microbiota composition.
基金supported by the National Natural Science Foundation of China(Grant No.32160532 and 31860446).
文摘Lactobacillus acidophilus GIM1.208(LA)can improve flavor,enhance antioxidant and hypoglycemic activities of Rosa roxburghii Tratt(RRT).This study investigated the interactions between proteins and active compounds in RRT fermented by LA to reveal the underlying reasons for changes in flavor and activity.A total of 6239 proteins were characterized and quantified by Label-free proteomics,with their molecular weights were mainly concentrated in the range of 10-40 kDa.The 129 differential proteins screened were mainly enriched in compounds synthesis or transformation pathways,such as glycolysis.Integrated analysis of proteomics and metabolomics revealed a strong correlation between proteins and metabolites,especiallyβ-glucosidase was closely correlation with flavonoids and triterpenoids.Additionally,molecular dynamics(MD)simulations have demonstrated that glycosides establish hydrogen bonds with the amino acids ofβ-glucosidase,while hydrophobic interactions serve as the primary driving force behind the binding process.Notably,Rosamutin exhibited the most significant binding energy withβ-glucosidase(66.257 kJ/mol).The results of the MD analysis further demonstrate the huge potential of LAβ-glucosidase to transform glycosides in RRT.In summary,proteins in LA fermented RRT contribute to the consumption or synthesis of compounds,ultimately enhancing the flavor and activity of RRT.This study’s results can provide a reference for the research of proteins and bioactive compounds of RRT.
基金supported by the National Natural Science Foundation of China(No.32060536)Science and Technology Program of Guizhou Province(Zk[2022]key 005).
文摘Dietary fiber(DF),an indigestible component of plant cell walls,is widely recognized for its health benefits.Rosa roxburghii Tratt(RRT)pomace contains high levels of DF,known for their anti-inflammatory and antioxidant properties,which are beneficial in managing atherosclerosis and diabetes;however,knowledge about the DF in RRT is limited.This study aimed to determine whether bound polyphenols(BPs)contribute to the health benefits of RRT’s DF and assess its potential as a valuable source of DF.DF was extracted from RRT(RRT-DF)using an enzymatic method and BPs removed from RRT-DF through alkaline extraction(RRT-DF-F),and the micro-structural,physicochemical,antioxidant,and adsorption properties were compared between RRT-DF and RRTDFF.The results showed no significant alteration in the microstructure,crystalline peaks,or characteristic bonds of the obtained fibers,and RRT-DF showed improved water-and oil-holding capacities.RRT-DF-F demonstrated significantly lower DPPH and OH-scavenging activity than RRT-DF,and the adsorption capacity for nitrite,cholesterol,and bile acid,and theα-glucosidase activity inhibition ratio was significantly decreased.These findings indicate that BPs contribute to the in vitro physicochemical and functional properties of RRT-DF,highlighting its potential as a functional food ingredient and supporting the high-value utilization of RRT byproducts.
基金supported by the Basic Research Projects of Free Exploration Category of Central Guided Local Science and Technology Development Funds(No.202407AA110007)the Yunnan Provincial Talent Reserve Project for Young and Middle-aged Academic and Technical Leaders(No.202405AC350032)+1 种基金the Ten Thousand People Plan of Yunnan Province,Young Top-notch Personnel(No.YNWR-QNBJ-2018-046)the Yunnan Fundamental Research Projects(No.202401AS070012),China。
文摘Objectives To enhance the stability of proliposomes co-encapsulating Rosa roxburghii Tratt seed oil(RSO)andβ-carotene(βC),sodium alginate(SA)and chitosan(CS)were utilized for bilayer surface modification.In this study,the proliposomes containing RSO andβC are referred to as Ps,while the modified formulations are denoted as sodium alginate-chitosanmodified proliposomes(SA-CS-Ps).Materials and methods The physicochemical properties,structural characteristics,thermal stability,microstructure,rheological behavior,storage stability,and oxidation kinetics of SA-CS-Ps were investigated and compared with those of unmodified Ps and CS monolayer-modified Ps(CS-Ps).Results The particle size and turbidity of Ps increased with the addition of modification layers,reaching 563.73 nm and0.697,respectively,for SA-CS-Ps.CS and SA formed a protective bilayer through electrostatic interactions,which preserved the spherical structure of Ps while enhancing its apparent viscosity and stress.Thermal stability analysis indicated that the phase transition temperatures of CS-Ps and SA-CS-Ps were elevated by 10.57 and 15.76℃,respectively,compared with that of unmodified Ps.During storage,the retention rates(RRs)of RSO andβC followed the order SA-CS-Ps>CS-Ps>Ps,whereas malondialdehyde and peroxide values exhibited the opposite trend.Elevated temperatures accelerated oxidation,thereby compromising the protective effect of the carriers.After 24 d at 4℃,SA-CSPs demonstrated superior stability,with malondialdehyde of 0.016μg/mg,peroxide value of 2.697 mmol/kg,and RRs of RSO of 97.15%andβC of 96.37%.The oxidation kinetics conformed to a first-order reaction model.Conclusions Given its enhanced protection against degradation and stability,SA-CS-Ps represents a promising candidate for the delivery of sensitive bioactive compounds.This study provides a theoretical basis for the application of bilayer-modified proliposomes in functional food delivery systems requiring enhanced stability.
基金National Natural Science Foundation of China(Grant No.32260560,32301997)Guizhou Provincial Science and Technology Project(Grant No.ZK[2022]047,[2023]092)+1 种基金Guizhou Provincial Science and Technology Support Project(Grant No.[2023]343)Guizhou University Natural Science Special Scientific Research Fund Project(X2021327).
文摘The key polyphenolic compounds in polyphenol extracts from Rosa roxburghii distiller’s grains(R.roxburghii DGs)were crucial in regulating intestinal homeostasis effectively.Thus,an integrative analysis combining metagenomics and metabolomics investigated the interactions and mechanisms between the combination of key polyphenols from R.roxburghii DGs(CKPR)and the fecal microbiota.The free polyphenols in CKPR(CKPR-FP)regulated intestinal homeostasis more effectively than the bound polyphenols in CKPR(CKPR-BP)by decreasing the Bacteroidetes/Firmicutes(B/F)ratio and targeted modulating gut microbiota.Gut microbiota metabolized CKPR into small-molecule bioactives,maintaining microecological stability and contributing to intestinal functions.Additionally,hydroxyphenyl propionic acid and phenylacetic acid stimulated microbial enzymatic pathways related to the degradation of polycyclic aromatic hydrocarbons,xylene,and methyl anthranilate.This research reveals the potential of CKPR and fecal microbiota interaction in maintaining host health.It provides a reference for the precise development of functional foods and nutritional supplements.
基金supported by the National Key Research and Development Program of China(2024YFD1300803)the Fundamental Research Funds for the Central Universities(2572023AW35)National Natural Science Foundation of China(32501698).
文摘Rosa roxburghii Tratt.(Cili)is a species of rose fruit with distinctive characteristics,combining medicinal and edible values.In recent years,it has become the focus of attention in the field of nutrition and health.From a health perspective,the core advantage of R.roxburghii lies in its high concentration of bioactive substances.Among these substances,pentacyclic triterpenoids are regarded as one of the key substances that contribute to its health-promoting effects.However,the regulatory mechanisms of triterpenoid biosynthesis remain unclear.The present study found that ultraviolet-B(UV-B)radiation increased the total triterpene content of R.roxburghii by 1.49-fold.The key cyclase gene RrOSC4 encodes an oxidosqualene cyclase implicated in the formation ofα/β-amyrin skeletons under UV-B radiation.Co-expression network and promoter analyses identified RrMYB62 as a key regulator of triterpenoid biosynthesis.Furthermore,over-expression of RrMYB62 upregulated the expression of RrOSC4,leading to increased pentacyclic triterpenoids content in‘NC89’tobacco leaves.Results from yeast one-hybrid,dual-luciferase activity and electrophoretic mobility shift assays demonstrated that RrMYB62 directly binds and activates the promoter of RrOSC4 to regulate triterpenoid biosynthesis.Taken together,our findings demonstrate that triterpenoid biosynthesis in R.roxburghii exposed to UV-B radiation is regulated by the RrMYB62-RrOSC4 module.This study revealed the interplay between light signaling and functional phytochemicals,offering a molecular basis for enhancing the fruit quality in the field of food application.
基金supported by the Qiannan Prefecture Science and Technology Support Grant Project(Qiannankehe Support[2024]No.16)the Guizhou Provincial Science and Technology Support Grant Project(Qiankehe Support[2023]No.472 and[2020]No.1Y148)the Guizhou Province Science and Technology Program Funding Project(Qian Kehe Basic-2K[2022]No.445),China.
文摘With growing concerns about the negative impact of environmental pollution on skin health,there is an increasing demand for natural active ingredients to combat free radicals and excessive melanin production.In this context,Rosa roxburghii Tratt(RR)has emerged as a potential source of skin-lightening compounds.Vitamin C(Vc)and superoxide dismutase(SOD)-rich extracts were extracted from RR,namely RRVC and RRSOD,using macroporous resin column chromatography and graded ammonium sulfate precipitation.The antioxidant and skin-lightening effects of these extracts were evaluated.RRVC and RRSOD showed strong inhibitory activity against various radicals and tyrosinase.When formulated together,their antioxidant and skin-lightening abilities were significantly enhanced,with the optimal ratio of RRVC and RRSOD(RRVS)of 5:32.In vivo studies on C57BL/6J mice revealed that these two extracts and their formulations effectively lowered oxidative stress levels in a model of d-galactose-induced aging in mice.Furthermore,they inhibited melanogenesis and tyrosinase activity in B16 cells and reduced ultravioletradiation-induced melanin deposition in mouse ears.The combination of RRVC and RRSOD had a more potent melanin-scavenging effect compared with the individual extracts.These findings suggest that RRVC and RRSOD,especially when combined at the optimal ratio,can whiten skin,increasing the value of RR products and offering natural alternatives for skin lightening.
基金The National Key Research and Development Program of China(2017YFD0400503-3)Major Special Science and Technology Project of Yunnan Province(202002AE320006)+2 种基金National Natural Science Foundation of China(31760457,32101894,32160547)Vegetable Industry Technology System Post Expert Project of Jiangxi Province(JXARS-06)State Key Laboratory of Food Science and Resources Nanchang University(SKLF-ZZA-201910,SKLF-ZZB-202132)are gratefully acknowledged.
文摘Rosa roxburghii Tratt(RRT)has been widely studied in recent years due to its rich nutritional composition.In this study,the effects of fermentation by Limosilactobacillus fermentum NCU001464(Lm.fermentum NCU001464)on the physicochemical properties and biological activity of dried RRT fruit were investigated.The pH and monosaccharide content of RRT decreased after 48 h of fermentation,the total phenolic content(TPC)increased from 15.06 to 21.74μmol GAE/L,the antioxidant capacity increased and xanthine oxidase(XO)inhibition increased from 25.89 to 36.67%.Moreover,the half-maximal inhibitory concentration(IC50)for XO,of the fermented RRT phenol extract was lower than that of the unfermented phenol extract.Fluorescence spectroscopy confirmed the stronger,inhibitory,binding interactions between XO and fermented RRT.The main binding forces between fermented RRT phenol extract and XO appeared to be hydrogen bonds and van der Waals forces.
基金supported by the National Natural Science Foundation of China(Grant No.32160532 and 31860446).
文摘Lactobacillus acidophilus GIM1.208(LA)can greatly improve the volatile flavor and sensory scores of Rosa roxburghii Tratt(RRT),while its effects on nonvolatile flavor and active compounds remains uncertain.Therefore,this study used widely targeted metabolomics to comprehensively analyze the effects of LA on flavor and activity of RRT.After being fermented by LA,the total phenolics and flavonoids content of RRT increased,while the total triterpenoids content slightly decreased.The total antioxidant activity rose by 35.23%,and hypoglycemic activity expressed through α-amylase and α-glucosidase inhibitory activities increased by 51.66% and 24.95%,respectively.A total of 1635 metabolites were identified,including amino acids,phenolic acids,flavonoids,and triterpenes,and 205 different metabolites were screened.The analysis of potential taste-related metabolites among differential metabolites revealed a reduction in amino acid content,while the majority of organic acids and sugars exhibited an increase.The electronic-tongue and electronic-nose showed that LA reduces bitterness and astringency while enhancing the aroma of RRT,indicating changes in flavor compounds lead to improved flavor quality.Additionally,LA can hydrolyze glycoside compounds in RRT and release glycosides with higher activity,which contributes to its increased antioxidant and hypoglycemic effects.In summary,LA induced alterations in the flavor compounds and glycosides of RRT,thereby improved its flavor and activity.This study can serve as a reference for future research on the flavor and activity of RRT,as well as for production quality control.
