The orbitofrontal cortex(ORB),a region crucial for stimulus-reward association,decision-making,and flexible behaviors,extensively connects with other brain areas.However,brain-wide inputs to projection-defined ORB neu...The orbitofrontal cortex(ORB),a region crucial for stimulus-reward association,decision-making,and flexible behaviors,extensively connects with other brain areas.However,brain-wide inputs to projection-defined ORB neurons and the distribution of inhibitory neurons postsynaptic to neurons in specific ORB subregions remain poorly characterized.Here we mapped the inputs of five types of projection-specific ORB neurons and ORB outputs to two types of inhibitory neurons.We found that different projection-defined ORB neurons received inputs from similar cortical and thalamic regions,albeit with quantitative variations,particularly in somatomotor areas and medial groups of the dorsal thalamus.By counting parvalbumin(PV)or somatostatin(SST)interneurons innervated by neurons in specific ORB subregions,we found a higher fraction of PV neurons in sensory cortices and a higher fraction of SST neurons in subcortical regions targeted by medial ORB neurons.These results provide insights into understanding and investigating the function of specific ORB neurons.展开更多
In the past decade, the rapid develop-ment of viral tracing techniques, more precisely the neu-rotropic viral based mono-transsynaptic tracing strategy,provides a powerful tool to map synaptic connectivi...In the past decade, the rapid develop-ment of viral tracing techniques, more precisely the neu-rotropic viral based mono-transsynaptic tracing strategy,provides a powerful tool to map synaptic connectivityin the central and peripheral nervous systems. This minireview provides a brief description of the principles ofthis tracing method, describe different strategies to tracethe neural circuit from defned neuronal populations, andpresent an update of newly developed viral variants optimizing the method.展开更多
基金supported by the STI2030-Major Projects(2021ZD0203700/2021ZD0203703)the National Natural Science Foundation of China(31771151 and 32171030 to H.Y.,32100829 to D.L.)+2 种基金the Lingang Lab(Grant LG202104-01-03)Shanghai Municipal Science and Technology Major Project(grant No.2018SHZDZX05)the Strategic Priority Research Program of the Chinese Academy of Sciences(grant No.XDB32010200).
文摘The orbitofrontal cortex(ORB),a region crucial for stimulus-reward association,decision-making,and flexible behaviors,extensively connects with other brain areas.However,brain-wide inputs to projection-defined ORB neurons and the distribution of inhibitory neurons postsynaptic to neurons in specific ORB subregions remain poorly characterized.Here we mapped the inputs of five types of projection-specific ORB neurons and ORB outputs to two types of inhibitory neurons.We found that different projection-defined ORB neurons received inputs from similar cortical and thalamic regions,albeit with quantitative variations,particularly in somatomotor areas and medial groups of the dorsal thalamus.By counting parvalbumin(PV)or somatostatin(SST)interneurons innervated by neurons in specific ORB subregions,we found a higher fraction of PV neurons in sensory cortices and a higher fraction of SST neurons in subcortical regions targeted by medial ORB neurons.These results provide insights into understanding and investigating the function of specific ORB neurons.
文摘In the past decade, the rapid develop-ment of viral tracing techniques, more precisely the neu-rotropic viral based mono-transsynaptic tracing strategy,provides a powerful tool to map synaptic connectivityin the central and peripheral nervous systems. This minireview provides a brief description of the principles ofthis tracing method, describe different strategies to tracethe neural circuit from defned neuronal populations, andpresent an update of newly developed viral variants optimizing the method.
