Objective:To evaluate the nursing value of mechanical expectoration drainage combined with traditional Chinese medicine acupoint application in children with pediatric bronchial pneumonia.Methods:A total of 62 childre...Objective:To evaluate the nursing value of mechanical expectoration drainage combined with traditional Chinese medicine acupoint application in children with pediatric bronchial pneumonia.Methods:A total of 62 children with pediatric bronchial pneumonia treated from May 2024 to May 2025 were selected as samples and randomly divided into groups using a random number table.Group A received mechanical expectoration drainage combined with traditional Chinese medicine acupoint application nursing,while Group B received conventional nursing.Lung function,symptom disappearance time,nursing satisfaction,and inflammatory indicators were compared between the two groups.Results:The maximum vital capacity(FVC),forced expiratory volume in one second(FEV),peak expiratory flow(PEF),and maximal mid-expiratory flow(MMEF)in Group A were all higher than those in Group B,with p<0.05;the symptom disappearance time in Group A was shorter than that in Group B,and nursing satisfaction was higher in Group A than in Group B,with p<0.05;the procalcitonin(PCT),white blood cell count(WBC),and serum amyloid A(SAA)levels in Group A were lower than those in Group B,with p<0.05.Conclusion:The application of mechanical expectoration drainage combined with traditional Chinese medicine acupoint application nursing in the care of children with pediatric bronchial pneumonia reduces inflammation levels,shortens symptom disappearance time,and improves lung function.展开更多
Objective To systematically evaluate the overall efficacy of external application of traditional Chinese medicine(EA-TCM)in combination with oral three-step analgesic ladder therapy for patients suffering from cancer-...Objective To systematically evaluate the overall efficacy of external application of traditional Chinese medicine(EA-TCM)in combination with oral three-step analgesic ladder therapy for patients suffering from cancer-induced bone pain(CIBP).Methods We conducted a literature search of randomized controlled trials on the combination of EA-TCM and three-step analgesic ladder therapy for CIBP across ten databases and two registration systems.It included four Chinese databases[Chinese Biomedical Literature Database(SinoMed),China National Knowledge Infrastructure(CNKI),Wanfang Database,and China Science and Technology Journal Database(VIP)],six English databases(Scopus,Embase,Web of Science,PubMed,Cochrane Library,and OpenGrey),and two registration systems(Chinese Clinical Trial Registry and ClinicalTrials.gov).The timeframe for the literature search extended from the inception of each database to December 31,2023.Meta-analysis was performed using RevMan(v5.4.1),and the outcome indicators(pain relief rate,analgesic duration,quality of life,pain intensity,breakthrough pain frequency,and adverse reactions)were graded using GRADE profiler(v3.6).Results According to the established inclusion and exclusion criteria,a total of 43 studies was deemed eligible,involving 3142 participants with CIBP.The results of meta-analysis showed that compared with oral three-step analgesic ladder therapy alone,the combined therapy of EA-TCM and three-step analgesic ladder has a significant improvement in pain relief rate[risk ratio(RR)=1.32,95%confidence interval(CI):1.24 to 1.41,P<0.00001],analgesic duration[mean difference(MD)=1.33,95%CI:0.97 to 1.69,P<0.00001],and quality of life(MD=5.66,95%CI:4.88 to 6.44,P<0.00001).Furthermore,the combined therapy significantly reduced pain intensity(MD=-1.00,95%CI:-1.19 to-0.80,P<0.00001),breakthrough pain frequency(MD=-0.43,95%CI:-0.51 to-0.36,P<0.00001),and adverse reactions(RR=0.60,95%CI:0.53 to 0.68,P<0.00001)in CIBP patients.Based on the GRADE assessment,the level of evidence varied from low to moderate.Conclusion EA-TCM combined with the three-step analgesic ladder therapy can effectively alleviate pain symptoms in patients with CIBP and improve their quality of life.Additionally,the EA-TCM can effectively reduce the incidence of adverse reactions associated with threestep analgesic therapy.展开更多
Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Al...Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Almost half the recipients have been found to suffer from loss of graft function due to rejection reaction within 10 years of post-transplantation. Part of them have bad quality of life展开更多
Objective: for patients with peripheral facial paralysis, the clinical effect of combined treatment with external application of Chinese medicine acupoints and acupuncture in the treatment process is to provide basis ...Objective: for patients with peripheral facial paralysis, the clinical effect of combined treatment with external application of Chinese medicine acupoints and acupuncture in the treatment process is to provide basis for clinical treatment. Methods: the subjects of this research experiment were 120 patients with peripheral facial paralysis who received outpatient treatment in our hospital from January 2020 to December 2021. According to the order of admission, the patients were divided into two groups and then marked separately, and the clinical effects of different treatment methods were compared. Results: the treatment efficiency of the study group was higher than that of the control group, and the treatment duration was shorter than that of the control group (p < 0.05). Conclusion: for patients with peripheral facial paralysis, in the process of receiving clinical treatment, the combination of external application of traditional Chinese medicine acupoints and acupuncture has higher clinical effect, which can effectively shorten the treatment time of patients and is worthy of clinical promotion and application.展开更多
Objective: to analyze the clinical consequences of apoplectic arthritis in the course of treatment of Chinese drug topcoat. Methods: 100 patients with rheumatoid arthritis were cultured from January 20 to March 2021 a...Objective: to analyze the clinical consequences of apoplectic arthritis in the course of treatment of Chinese drug topcoat. Methods: 100 patients with rheumatoid arthritis were cultured from January 20 to March 2021 and treated in our hospital. 100 patients were randomly divided into two groups. The control group of 50 patients received routine treatment. A group of 50 patients with external coating of traditional Chinese medicine treatment, observe the symptoms and state indicators of the two groups. Results: after treatment, the difference between the disease index of the joint tumor obtained from the tissue and the joint pain index of the control group at dawn was statistical (N < 0.05). Conclusion: the application of external coating in the treatment of rheumatoid arthritis can quickly improve the clinical symptoms of patients with ideal effect, which is worthy of clinical publicity.展开更多
With the deepening of clinical medical research in recent years, among many pediatric diseases, diarrhea is a more common disease. After the onset of children, in addition to the increase of stool frequency and abnorm...With the deepening of clinical medical research in recent years, among many pediatric diseases, diarrhea is a more common disease. After the onset of children, in addition to the increase of stool frequency and abnormal stool characteristics, they are often combined with vomiting, abdominal pain and fever, which is very easy to cause water and electricity disorder and seriously affect the health and even life safety of children. Infectious diarrhea is the most common clinical diarrhea in children, which often occurs in summer and autumn. At present, the treatment mainly includes correcting water and electricity disorder, rational application of antibiotics, protection of gastrointestinal mucosa, intestinal probiotics, etc. the clinical curative effect is not satisfactory, and the problems such as bacterial drug resistance, double infection, flora imbalance, expensive treatment cost and easy repetition caused by unreasonable use of antibiotics are difficult to be completely solved. This paper analyzes the advantages of Acupoint Application of traditional Chinese medicine and western medicine in the treatment of infantile diarrhea.展开更多
This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application informa...This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application information was gathered from relevant reports,books,and classic material medica.Research literature and dissertations on C.axillaris chemical constituents and pharmacological activities,available up to 2023,were sourced from electronic databases.Pharmaceutical components such as flavonoids,phenolic acids,triterpenes,lignans,and organic acids were identified in C.axillaris and categorized based on their relevance to cardiovascular roles.Examining the material basis for C.axillaris efficacy in addressing cardiovascular diseases,an analysis of 27 Chinese patent medicines containing Choerospondiatis Fructus(CF)highlighted Myristicae Semen as the most frequently utilized and strongly associated with CF in prescriptions.C.axillaris exhibited notable pharmacological effects,encompassing anti-myocardial ischemia-reperfusion injury,anti-arrhythmic properties,anti-myocardial fibrosis,and hemorheological effects linked to cardiovascular diseases.This review aspires to provide valuable insights for translating traditional applications into modern pharmaceuticals and offers guidance for clinical applications in cardiovascular interventions.展开更多
Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away cold.It has been extensively utilized in ...Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away cold.It has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and toothache.This review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of EH.Relevant information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic databases.EH is a member of Compositae family and is mainly grown in southern China.Traditional Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving pain.By now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other compounds.EH extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative diseases.Scutellarin,the major active compound of EH,has been used as a quality marker.And no obvious toxicity of EH has been reported.According to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine fields.While several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain elusive.To fully exploit the medicinal potential of EH,further studies on understanding the effective material basis and mechanisms are warranted.展开更多
Daucus carota(D.carota)is rich phytochemical content and has potential pharmacological properties.The root of the carrot serves as a nutritious source of vitamins,particularly notable for its highβ-carotene content,w...Daucus carota(D.carota)is rich phytochemical content and has potential pharmacological properties.The root of the carrot serves as a nutritious source of vitamins,particularly notable for its highβ-carotene content,which is a precursor to vitamin A.Additionally,it is an excellent source of flavonoids,polyacetylenes,phenolic acids,and carotenoids.These phytochemicals are associated with numerous health benefits,including antioxidant,anti-inflammatory,antimicrobial,anticancer,and hepatoprotective effects.Recent research has expanded our knowledge of these bioactive compounds,providing insights into theirmolecular mechanisms of action.This information underscores the relevance of the antioxidant and anti-inflammatory properties of carrot phytochemicals in the prevention and management of chronic diseases such as anti-inflammatory activity,antioxidant activity,hepatoprotective activity,antifungal activity,antimicrobial activity,antibacterial activity,anticancer activity,antidiabetic activity,antiulcer activity,cardioprotective activity,antidepressant activity,antidementia effect,and analgesic effect.Moreover,the antimicrobial and immunomodulatory properties of carrot extracts suggest their potential applications in traditional medicine and as complementary treatments in contemporary therapeutic practices.展开更多
[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control gr...[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.展开更多
文摘Objective:To evaluate the nursing value of mechanical expectoration drainage combined with traditional Chinese medicine acupoint application in children with pediatric bronchial pneumonia.Methods:A total of 62 children with pediatric bronchial pneumonia treated from May 2024 to May 2025 were selected as samples and randomly divided into groups using a random number table.Group A received mechanical expectoration drainage combined with traditional Chinese medicine acupoint application nursing,while Group B received conventional nursing.Lung function,symptom disappearance time,nursing satisfaction,and inflammatory indicators were compared between the two groups.Results:The maximum vital capacity(FVC),forced expiratory volume in one second(FEV),peak expiratory flow(PEF),and maximal mid-expiratory flow(MMEF)in Group A were all higher than those in Group B,with p<0.05;the symptom disappearance time in Group A was shorter than that in Group B,and nursing satisfaction was higher in Group A than in Group B,with p<0.05;the procalcitonin(PCT),white blood cell count(WBC),and serum amyloid A(SAA)levels in Group A were lower than those in Group B,with p<0.05.Conclusion:The application of mechanical expectoration drainage combined with traditional Chinese medicine acupoint application nursing in the care of children with pediatric bronchial pneumonia reduces inflammation levels,shortens symptom disappearance time,and improves lung function.
基金Provincial Key Research and Development Project of Hunan(2018SK2127)Hunan Province Traditional Chinese Medicine Research and Development Project(201946).
文摘Objective To systematically evaluate the overall efficacy of external application of traditional Chinese medicine(EA-TCM)in combination with oral three-step analgesic ladder therapy for patients suffering from cancer-induced bone pain(CIBP).Methods We conducted a literature search of randomized controlled trials on the combination of EA-TCM and three-step analgesic ladder therapy for CIBP across ten databases and two registration systems.It included four Chinese databases[Chinese Biomedical Literature Database(SinoMed),China National Knowledge Infrastructure(CNKI),Wanfang Database,and China Science and Technology Journal Database(VIP)],six English databases(Scopus,Embase,Web of Science,PubMed,Cochrane Library,and OpenGrey),and two registration systems(Chinese Clinical Trial Registry and ClinicalTrials.gov).The timeframe for the literature search extended from the inception of each database to December 31,2023.Meta-analysis was performed using RevMan(v5.4.1),and the outcome indicators(pain relief rate,analgesic duration,quality of life,pain intensity,breakthrough pain frequency,and adverse reactions)were graded using GRADE profiler(v3.6).Results According to the established inclusion and exclusion criteria,a total of 43 studies was deemed eligible,involving 3142 participants with CIBP.The results of meta-analysis showed that compared with oral three-step analgesic ladder therapy alone,the combined therapy of EA-TCM and three-step analgesic ladder has a significant improvement in pain relief rate[risk ratio(RR)=1.32,95%confidence interval(CI):1.24 to 1.41,P<0.00001],analgesic duration[mean difference(MD)=1.33,95%CI:0.97 to 1.69,P<0.00001],and quality of life(MD=5.66,95%CI:4.88 to 6.44,P<0.00001).Furthermore,the combined therapy significantly reduced pain intensity(MD=-1.00,95%CI:-1.19 to-0.80,P<0.00001),breakthrough pain frequency(MD=-0.43,95%CI:-0.51 to-0.36,P<0.00001),and adverse reactions(RR=0.60,95%CI:0.53 to 0.68,P<0.00001)in CIBP patients.Based on the GRADE assessment,the level of evidence varied from low to moderate.Conclusion EA-TCM combined with the three-step analgesic ladder therapy can effectively alleviate pain symptoms in patients with CIBP and improve their quality of life.Additionally,the EA-TCM can effectively reduce the incidence of adverse reactions associated with threestep analgesic therapy.
文摘Kidney transplantation is one of the important means in saving the life of patients with renal diseases of the terminal stage. However, rejection reaction often results in the loss of the grafted organs’ function. Almost half the recipients have been found to suffer from loss of graft function due to rejection reaction within 10 years of post-transplantation. Part of them have bad quality of life
文摘Objective: for patients with peripheral facial paralysis, the clinical effect of combined treatment with external application of Chinese medicine acupoints and acupuncture in the treatment process is to provide basis for clinical treatment. Methods: the subjects of this research experiment were 120 patients with peripheral facial paralysis who received outpatient treatment in our hospital from January 2020 to December 2021. According to the order of admission, the patients were divided into two groups and then marked separately, and the clinical effects of different treatment methods were compared. Results: the treatment efficiency of the study group was higher than that of the control group, and the treatment duration was shorter than that of the control group (p < 0.05). Conclusion: for patients with peripheral facial paralysis, in the process of receiving clinical treatment, the combination of external application of traditional Chinese medicine acupoints and acupuncture has higher clinical effect, which can effectively shorten the treatment time of patients and is worthy of clinical promotion and application.
文摘Objective: to analyze the clinical consequences of apoplectic arthritis in the course of treatment of Chinese drug topcoat. Methods: 100 patients with rheumatoid arthritis were cultured from January 20 to March 2021 and treated in our hospital. 100 patients were randomly divided into two groups. The control group of 50 patients received routine treatment. A group of 50 patients with external coating of traditional Chinese medicine treatment, observe the symptoms and state indicators of the two groups. Results: after treatment, the difference between the disease index of the joint tumor obtained from the tissue and the joint pain index of the control group at dawn was statistical (N < 0.05). Conclusion: the application of external coating in the treatment of rheumatoid arthritis can quickly improve the clinical symptoms of patients with ideal effect, which is worthy of clinical publicity.
文摘With the deepening of clinical medical research in recent years, among many pediatric diseases, diarrhea is a more common disease. After the onset of children, in addition to the increase of stool frequency and abnormal stool characteristics, they are often combined with vomiting, abdominal pain and fever, which is very easy to cause water and electricity disorder and seriously affect the health and even life safety of children. Infectious diarrhea is the most common clinical diarrhea in children, which often occurs in summer and autumn. At present, the treatment mainly includes correcting water and electricity disorder, rational application of antibiotics, protection of gastrointestinal mucosa, intestinal probiotics, etc. the clinical curative effect is not satisfactory, and the problems such as bacterial drug resistance, double infection, flora imbalance, expensive treatment cost and easy repetition caused by unreasonable use of antibiotics are difficult to be completely solved. This paper analyzes the advantages of Acupoint Application of traditional Chinese medicine and western medicine in the treatment of infantile diarrhea.
基金supported by grants from the Macao Science and Technology Development Fund(0043/2021/AGJ)the Science and Technology Major Project of Tibetan Autonomous Region of China(XZ202201ZD0001G06)+2 种基金the Natural Science Foundation of Sichuan Province(2022NSFSC1605)the Special Fund of Natural Science for the Central Universities(ZYN2023099)the Engineering Research Center for Pharmaceuticals and Equipments of Sichuan Province Fund(ERCPEF2201)。
文摘This article conducts a comprehensive review of both traditional therapeutic application knowledge and contemporary literature pertaining to Choerospondias axillaris(Roxb.)Burtt et Hill.Traditional application information was gathered from relevant reports,books,and classic material medica.Research literature and dissertations on C.axillaris chemical constituents and pharmacological activities,available up to 2023,were sourced from electronic databases.Pharmaceutical components such as flavonoids,phenolic acids,triterpenes,lignans,and organic acids were identified in C.axillaris and categorized based on their relevance to cardiovascular roles.Examining the material basis for C.axillaris efficacy in addressing cardiovascular diseases,an analysis of 27 Chinese patent medicines containing Choerospondiatis Fructus(CF)highlighted Myristicae Semen as the most frequently utilized and strongly associated with CF in prescriptions.C.axillaris exhibited notable pharmacological effects,encompassing anti-myocardial ischemia-reperfusion injury,anti-arrhythmic properties,anti-myocardial fibrosis,and hemorheological effects linked to cardiovascular diseases.This review aspires to provide valuable insights for translating traditional applications into modern pharmaceuticals and offers guidance for clinical applications in cardiovascular interventions.
基金funded by the State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources(Guangxi Normal University)(CMEMR2022-B11)the Natural Science Research of Jiangsu Higher education Institution of China(22KJB360018)Jiangsu Province University Student Innovation and Entrepreneurial Training Program(202311117019Z).
文摘Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away cold.It has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and toothache.This review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of EH.Relevant information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic databases.EH is a member of Compositae family and is mainly grown in southern China.Traditional Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving pain.By now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other compounds.EH extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative diseases.Scutellarin,the major active compound of EH,has been used as a quality marker.And no obvious toxicity of EH has been reported.According to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine fields.While several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain elusive.To fully exploit the medicinal potential of EH,further studies on understanding the effective material basis and mechanisms are warranted.
文摘Daucus carota(D.carota)is rich phytochemical content and has potential pharmacological properties.The root of the carrot serves as a nutritious source of vitamins,particularly notable for its highβ-carotene content,which is a precursor to vitamin A.Additionally,it is an excellent source of flavonoids,polyacetylenes,phenolic acids,and carotenoids.These phytochemicals are associated with numerous health benefits,including antioxidant,anti-inflammatory,antimicrobial,anticancer,and hepatoprotective effects.Recent research has expanded our knowledge of these bioactive compounds,providing insights into theirmolecular mechanisms of action.This information underscores the relevance of the antioxidant and anti-inflammatory properties of carrot phytochemicals in the prevention and management of chronic diseases such as anti-inflammatory activity,antioxidant activity,hepatoprotective activity,antifungal activity,antimicrobial activity,antibacterial activity,anticancer activity,antidiabetic activity,antiulcer activity,cardioprotective activity,antidepressant activity,antidementia effect,and analgesic effect.Moreover,the antimicrobial and immunomodulatory properties of carrot extracts suggest their potential applications in traditional medicine and as complementary treatments in contemporary therapeutic practices.
基金Supported by National Key R&D Program(2019YFC1712500)Science and Technology Program of Guizhou Province([2020]3003)Project of Guizhou University of Traditional Chinese Medicine(2018YFC170810520).
文摘[Objectives]To explore the protective effect and molecular mechanism of Shui People s Classic Prescription Jipei Dilong Ointment on knee osteoarthritis in rats.[Methods]72 SPF male SD rats were divided into control group,model group,Jipei Dilong Ointment high,medium and low dose groups,and positive drug Diclofenac group,with 12 rats in each group.Except the control group,all other groups were injected with 0.2 mL of 2%papain and 0.3%L-cysteine mixture into knee joint cavity to establish osteoarthritis model,while the control group was injected with the same amount of normal saline.The Lequesne MG score was used to determine the success of the model.After successful modeling,external administration was given for 4 weeks.The histopathological changes in articular cartilage and synovium were observed by HE staining;the levels of TNF-α,IL-1βand COX-2 in serum were detected by ELISA,and the relative expression of MMP-9 and TIMP-1 mRNA in cartilage was detected by qRT-PCR;the relative expression of MMP-9,TIMP-1,TLR4,MyD88 and NF-κB in rat cartilage was detected by Western-blot.[Results]Compared with the model group,Jipei Dilong Ointment could significantly reduce the Lequesne MG score and Mankin s score of arthritic rats(P<0.01);significantly improve the pathological changes in articular cartilage and synovium,reduce tissue edema,necrosis,inflammatory cell infiltration and fibrous tissue proliferation;reduce the expression of MMP-9 mRNA(P<0.01)in different degrees and increase the expression of TIMP-1 mRNA(P<0.01);reduce the relative expression of MMP-9,TLR4,MyD88 and NF-κB protein in different degrees(P<0.01),and significantly increase the relative expression of TIMP-1 protein in cartilage(P<0.01).[Conclusions]Jipei Dilong Ointment can improve the joint injury of osteoarthritis rats,and the mechanism may be related to the regulation of TLR4/MyD88/NF-κB signaling pathway and the ratio of MMP-9/TIMP-1.
