How temperature affects the direction of sexual differentiation pathways in temperature-dependent sex determination(TSD)remains a mystery despite decades of scientific investigation.In the accompanying study,we demons...How temperature affects the direction of sexual differentiation pathways in temperature-dependent sex determination(TSD)remains a mystery despite decades of scientific investigation.In the accompanying study,we demonstrated the thermal switch mechanism within thermosensitive steroidogenic cells(TSSCs)that triggers the male development pathway in turtles(Trachemys scripta elegans).However,the transcriptional regulatory mechanisms linking TSSCs'cold sensitivity with pre-Sertoli cell specification remain elusive.Herein,we elucidate the regulatory schema governing the male determination pathway,including Ca^(2+)-induced aldosterone synthesis in TSSCs and subsequent aldosterone-driven transcriptional modulation in pre-supporting cells.Aldosterone-bound NR3C1,a master regulator of the male determination pathway,activates DMRT1 gene expression,culminating in pre-Sertoli specification.Therefore,this study discovers a novel male-specific pathway by which temperature initiates male sex determination in TSD species.展开更多
基金supported by the National Natural Science Foundation of China(32030013,31821001,32430012,32170486,and 32371570)the National Key R&D Program of China(2023YFF1304900)+4 种基金the Heilongjiang Provincial Natural Science Foundation for Outstanding Youths(JQ2021C001)the National Forestry and Grassland Administration(2020132610)the Heilongjiang Provincial Natural Science Foundation for Excellent Young Scientists(YQ2023C022)the Heilongjiang Chunyan Support Program for Young Scientific and Technological Talents Team(CYQN24058)the Fundamental Research Funds for the Central Universities(2572023CT13-2).
文摘How temperature affects the direction of sexual differentiation pathways in temperature-dependent sex determination(TSD)remains a mystery despite decades of scientific investigation.In the accompanying study,we demonstrated the thermal switch mechanism within thermosensitive steroidogenic cells(TSSCs)that triggers the male development pathway in turtles(Trachemys scripta elegans).However,the transcriptional regulatory mechanisms linking TSSCs'cold sensitivity with pre-Sertoli cell specification remain elusive.Herein,we elucidate the regulatory schema governing the male determination pathway,including Ca^(2+)-induced aldosterone synthesis in TSSCs and subsequent aldosterone-driven transcriptional modulation in pre-supporting cells.Aldosterone-bound NR3C1,a master regulator of the male determination pathway,activates DMRT1 gene expression,culminating in pre-Sertoli specification.Therefore,this study discovers a novel male-specific pathway by which temperature initiates male sex determination in TSD species.
