AIMS The paper studies the relationship between spleen deficiency substance,spleen deficiency and gastric cancer. METHODS We adopted the IBAS 2000 image analysis system,501B SEM with 9100/60 energy chromatic dispersin...AIMS The paper studies the relationship between spleen deficiency substance,spleen deficiency and gastric cancer. METHODS We adopted the IBAS 2000 image analysis system,501B SEM with 9100/60 energy chromatic dispersing X-ray analysis instrument technique,histologic chemistry and radio-immunity means to assay the ultramicro structure,in- testinal rnetaplasia subtypes,cAMP,DNA,trace element series and their oxides of the patients' gastric mucosa. RESULTS The incidence rates of gastric cancer,incom- plete colonic intestinal metaplasia and“background lesion”of spleen deficiency with Qi stagnation patients are remarkably higher than those of spleen Qi deficiency patients(P<0.05- 0.001 ).The levels of gastric mucosa cAMP,Zn,Cu,ZnO and CuO decreased from complete to incomplete intestinal meta- plasia and from small to colonic intestinal metaplasia,while DNA increased in the above sequence(P<0.05-0.001 ).The lev- els of DNA,cAMP,Zn,Cu,ZnO and CuO in gastric mucosa of incomplete colonic intestinal metaplasia tissue are not remark- ably different from those in gastric cancer tissue. CONCLUSION Gastric diseases of spleen dificiency with Qi stagnation has the tendency of cancerization; There is a close relationship between the incidence of incomplete colonic intestinal metaplasia and gastric cancer.展开更多
In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired...In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.展开更多
To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and ...To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and Ni in China’s Dexing copper mining district through paired sampling of water-amphibians,soil-earthworms,and air-lichens.Advanced methodologies were employed,including ICP-MS quantification for heavy metals,geochemical indices(Igeo,BCF,BAF)to assess bioavailability,NMDS for source apportionment,and HPLC to detect DNA methylation alterations.Aquatic systems exhibited severe Cd/Pb enrichment(16.25-24.42μg/L;11-15×WHO limits),while agricultural soils showed extreme Cd contamination(1.5 mg/kg;15×background).Biota displayed metal-specific accumulation:frogs achieved BCFs>1,000 for Pb/Cd,earthworms showed pH-modulated BAFs>2.5 for Cd/As,and lichens recorded 100-1,000×atmospheric Cr enrichment.NMDS resolved three contamination pathways:mining-derived Cd/Pb/As(MDS1=2.56),atmospheric Cr(PC2=1.84),and geogenic Ni.Cd dominated ecological risks(Eri=554.25;RI 300),while atmospheric Cr drove carcinogenic risks(TCR=4.11×10^(-5))exceeding safety thresholds.The source-media-biota-risk framework pioneers the integration of geochemical transport with epigenetic toxicity biomarkers,demonstrating that sub-lethal Cd/Pb exposure induces genome-wide DNA hypomethylation(2.4%-6.6%reduction;ρ=−0.71 to−0.91).This paradigm shift prioritizes bioavailability-informed regulations over concentration-based metrics,offering actionable strategies for sustainable development goals-aligned mining pollution control.展开更多
目的：探索高GC含量DNA的PCR扩增条件，为扩增达托霉素生物合成基因簇及拼接奠定基础。方法：在PCR扩增体系中，使用高保真的聚合酶及添加不同浓度的DMSO、7-deaza-dGTP等增强剂，并选择合适的PCR循环程序，优化富含GC的DNA的PCR扩增条...目的：探索高GC含量DNA的PCR扩增条件，为扩增达托霉素生物合成基因簇及拼接奠定基础。方法：在PCR扩增体系中，使用高保真的聚合酶及添加不同浓度的DMSO、7-deaza-dGTP等增强剂，并选择合适的PCR循环程序，优化富含GC的DNA的PCR扩增条件。结果：向反应体系中额外添加1%~4%的DMSO可以显著提高富含GC的DNA的PCR扩增产物量，但会降低其特异性；7-deaza-dGTP可以提高扩增产物的特异性及保真度，但产量会有所下降。应用touch down PCR并在体系中添加7-deaza-dGTP能够提高扩增产物的特异性和产率，增加扩增的保真度。结论：应用优化的PCR扩增条件将所有达托霉素生物合成基因簇分段扩增出来，并可扩增出长达6 kb的片段，且序列完全正确，可以进行后续拼接。展开更多
文摘AIMS The paper studies the relationship between spleen deficiency substance,spleen deficiency and gastric cancer. METHODS We adopted the IBAS 2000 image analysis system,501B SEM with 9100/60 energy chromatic dispersing X-ray analysis instrument technique,histologic chemistry and radio-immunity means to assay the ultramicro structure,in- testinal rnetaplasia subtypes,cAMP,DNA,trace element series and their oxides of the patients' gastric mucosa. RESULTS The incidence rates of gastric cancer,incom- plete colonic intestinal metaplasia and“background lesion”of spleen deficiency with Qi stagnation patients are remarkably higher than those of spleen Qi deficiency patients(P<0.05- 0.001 ).The levels of gastric mucosa cAMP,Zn,Cu,ZnO and CuO decreased from complete to incomplete intestinal meta- plasia and from small to colonic intestinal metaplasia,while DNA increased in the above sequence(P<0.05-0.001 ).The lev- els of DNA,cAMP,Zn,Cu,ZnO and CuO in gastric mucosa of incomplete colonic intestinal metaplasia tissue are not remark- ably different from those in gastric cancer tissue. CONCLUSION Gastric diseases of spleen dificiency with Qi stagnation has the tendency of cancerization; There is a close relationship between the incidence of incomplete colonic intestinal metaplasia and gastric cancer.
基金This research was supported by the National Natural Science Foundation of China
文摘In the criminal cases of driving under the influence(DUI), DNA evidence can be collected from the deployed airbag of the motor vehicle and submitted to the crime lab for touch DNA analysis.The evidence can be acquired when the skin cells are observed on the surface of the airbag in a traffic accident. However, the low quantity or quality of the evidence collected from a crime scene prevents further identification analysis in many cases. In the current study, we reported a case of identifying touch DNA extraction from the shed skin cells from the deployed airbag of a motor vehicle. We managed to collect DNA evidence from the shed skin cells in an airbag using a proper approach of collection and extraction. The 5.87 ng of extracted DNA was sufficient for genotyping and forensic identification, which helped to identify the driver of the car in collision with a pier in the street. In DUI cases and other traffic accidents, therefore, the amount of touch DNA extracted from the deployed airbag can be sufficient for DNA marker genotyping and further analysis.
基金financially supported by the Fundation of Key Laboratory of Ministry of Natural Resources for Eco-geochemistry (ZSDHJJ202202)Geological Investigation and Evaluation of Shale Gas in Complex Structural Areas of the Middle Yangtze plate(DD20250200604) of China Geological Survey+1 种基金the Natural Science Foundation of Guangdong Province,China(2023A1515140061)the Dongguan Science and Technology of Social Development Program(20231800935842, 20231800940562).
文摘To address the critical gap in linking multi-compartmental transfer with risks of trace metals(Cd,Pb,As,Cr,Ni)in mining environments.This study systematically investigated the trans-media migration of Cd,Pb,As,Cr,and Ni in China’s Dexing copper mining district through paired sampling of water-amphibians,soil-earthworms,and air-lichens.Advanced methodologies were employed,including ICP-MS quantification for heavy metals,geochemical indices(Igeo,BCF,BAF)to assess bioavailability,NMDS for source apportionment,and HPLC to detect DNA methylation alterations.Aquatic systems exhibited severe Cd/Pb enrichment(16.25-24.42μg/L;11-15×WHO limits),while agricultural soils showed extreme Cd contamination(1.5 mg/kg;15×background).Biota displayed metal-specific accumulation:frogs achieved BCFs>1,000 for Pb/Cd,earthworms showed pH-modulated BAFs>2.5 for Cd/As,and lichens recorded 100-1,000×atmospheric Cr enrichment.NMDS resolved three contamination pathways:mining-derived Cd/Pb/As(MDS1=2.56),atmospheric Cr(PC2=1.84),and geogenic Ni.Cd dominated ecological risks(Eri=554.25;RI 300),while atmospheric Cr drove carcinogenic risks(TCR=4.11×10^(-5))exceeding safety thresholds.The source-media-biota-risk framework pioneers the integration of geochemical transport with epigenetic toxicity biomarkers,demonstrating that sub-lethal Cd/Pb exposure induces genome-wide DNA hypomethylation(2.4%-6.6%reduction;ρ=−0.71 to−0.91).This paradigm shift prioritizes bioavailability-informed regulations over concentration-based metrics,offering actionable strategies for sustainable development goals-aligned mining pollution control.
文摘目的：探索高GC含量DNA的PCR扩增条件，为扩增达托霉素生物合成基因簇及拼接奠定基础。方法：在PCR扩增体系中，使用高保真的聚合酶及添加不同浓度的DMSO、7-deaza-dGTP等增强剂，并选择合适的PCR循环程序，优化富含GC的DNA的PCR扩增条件。结果：向反应体系中额外添加1%~4%的DMSO可以显著提高富含GC的DNA的PCR扩增产物量，但会降低其特异性；7-deaza-dGTP可以提高扩增产物的特异性及保真度，但产量会有所下降。应用touch down PCR并在体系中添加7-deaza-dGTP能够提高扩增产物的特异性和产率，增加扩增的保真度。结论：应用优化的PCR扩增条件将所有达托霉素生物合成基因簇分段扩增出来，并可扩增出长达6 kb的片段，且序列完全正确，可以进行后续拼接。
