目的使用Micro-CT,探讨重组人甲状旁腺激素(rh PTH)在2月龄雄性大鼠骨折愈合过程中的作用。方法应用2月龄雄性SD大鼠建立单侧闭合性股骨骨折内固定模型,随机分为甲状旁腺素治疗组和对照组,术后分别皮下注射重组人甲状旁腺素(1-34)10μg/...目的使用Micro-CT,探讨重组人甲状旁腺激素(rh PTH)在2月龄雄性大鼠骨折愈合过程中的作用。方法应用2月龄雄性SD大鼠建立单侧闭合性股骨骨折内固定模型,随机分为甲状旁腺素治疗组和对照组,术后分别皮下注射重组人甲状旁腺素(1-34)10μg/(kg.d)或等剂量生理盐水安慰剂(对照组),分别于术后第2、7、14、21和42天取双侧股骨标本,分别行X线检查和Micro-CT观察,并进行骨结构的定量分析。结果术后第21天,甲状旁腺素组骨折断端两侧的骨痂之间连续性良好,骨折线已模糊不清。甲状旁腺组较对照组,在术后第42天骨体积(bone Volume,BV)、骨体积分数(BV/TV)、骨密度(bone mineral density,BMD)和骨小梁模式因子(trabecular bone pattern factor,Tb.Pf)明显高于对照组;而骨小梁分离度(trabecular separation,Tb.Sp)和各向异性的程度(degree of anisotropy,DA)低于对照组。结论低剂量重组人甲状旁腺激素可提高大鼠骨折BV、BV/TV、Tb.Pf和BMD而降低Tb.Sp和DA,从而促进骨折的愈合。展开更多
<strong>Background:</strong> <span style="font-family:""><span style="font-family:Verdana;">Cleft alveolus closure is a challenge for </span><span style="...<strong>Background:</strong> <span style="font-family:""><span style="font-family:Verdana;">Cleft alveolus closure is a challenge for </span><span style="font-family:Verdana;">cleft</span> <span style="font-family:Verdana;">surgeon</span><span style="font-family:Verdana;"> for many decades. Secondary alveolar bone grafting (SABG) is the preferred technique using autologous cancellous </span><span style="font-family:Verdana;">ileac</span><span style="font-family:Verdana;"> crest bone as the donor graft. Many alternative methods were tried over the years with no</span></span><span style="font-family:Verdana;"> <span style="font-family:Verdana;">any</span></span><span style="font-family:""><span style="font-family:Verdana;"> promising results. Gingivoperiosteoplasty (GPP) is a good alternative surgical technique for bone regeneration in cleft alveolus with proper case selection criteria. </span><b><span style="font-family:Verdana;">Aim:</span></b><span style="font-family:Verdana;"> Aim of the study was to evaluate the bone regeneration following </span><span style="font-family:Verdana;">closure</span><span style="font-family:Verdana;"> of cleft alveolus with </span><span style="font-family:Verdana;">gingivoperiosteoplasty</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Method: </span></b><span style="font-family:Verdana;">This study was carried out in the department of Oral and Maxillofacial Surgery, Yenepoya Dental College, Deralakatte, Mangalore, Karnataka, India, from March 2018 to July 2019. In this </span><span style="font-family:Verdana;">study</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> we have performed </span><span style="font-family:Verdana;">gingivoperiosteoplasty</span><span style="font-family:Verdana;"> with palatoplasty and evaluated the amount of bone formation in the cleft alveolus using a series of </span><span style="font-family:Verdana;">intra oral</span><span style="font-family:Verdana;"> periapical radiographs. Five patients who required surgical repair of unilateral cleft palate and alveolus were selected for the study. </span><span style="font-family:Verdana;">Clinical</span><span style="font-family:Verdana;"> and radiographical assessment was done </span><span style="font-family:Verdana;">post operatively</span><span style="font-family:Verdana;">, after three and six months respectively for anatomical function and bone formation. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> After completion of clinical studies on patients</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> the statistical analysis of the data obtained. Radiographs were </span><span style="font-family:Verdana;">analy</span></span><span style="font-family:Verdana;">z</span><span style="font-family:Verdana;">ed</span><span style="font-family:""><span style="font-family:Verdana;"> for </span><span style="font-family:Verdana;">grey scale</span><span style="font-family:Verdana;"> density </span><span style="font-family:Verdana;">by means of</span><span style="font-family:Verdana;"> adobe </span><span style="font-family:Verdana;">photo shop</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">using</span><span style="font-family:""><span style="font-family:Verdana;"> the MATLAB process by histogram </span><span style="font-family:Verdana;">comparison</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">in</span><span style="font-family:""><span style="font-family:Verdana;"> three months and six months, </span><a name="_Hlk58658647"></a><span style="font-family:Verdana;">showing denser </span><span style="font-family:Verdana;">grey scale</span><span style="font-family:Verdana;"> pattern, indicating the new bone formation in the cleft alveolus surgical site, in all the 5 surgical cases. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">Gingivoperiosteoplasty (GPP) is a good alternative procedure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> secondary alveolar bone grafting (SABG), so donor site morbidity can be avoided with reduced time and cost for the surgery. But a larger sample size and longer follow-up </span></span><span style="font-family:Verdana;">are</span><span style="font-family:""><span style="font-family:Verdana;"> necessary to understand the better reliability of this surgical technique. </span><b><span style="font-family:Verdana;">Clinical Significance: </span></b><span style="font-family:Verdana;">Gingivoperiosteoplasty in cleft alveolus patients showed significant bone formation in 3 to 6 months postoperatively.</span></span>展开更多
文摘目的使用Micro-CT,探讨重组人甲状旁腺激素(rh PTH)在2月龄雄性大鼠骨折愈合过程中的作用。方法应用2月龄雄性SD大鼠建立单侧闭合性股骨骨折内固定模型,随机分为甲状旁腺素治疗组和对照组,术后分别皮下注射重组人甲状旁腺素(1-34)10μg/(kg.d)或等剂量生理盐水安慰剂(对照组),分别于术后第2、7、14、21和42天取双侧股骨标本,分别行X线检查和Micro-CT观察,并进行骨结构的定量分析。结果术后第21天,甲状旁腺素组骨折断端两侧的骨痂之间连续性良好,骨折线已模糊不清。甲状旁腺组较对照组,在术后第42天骨体积(bone Volume,BV)、骨体积分数(BV/TV)、骨密度(bone mineral density,BMD)和骨小梁模式因子(trabecular bone pattern factor,Tb.Pf)明显高于对照组;而骨小梁分离度(trabecular separation,Tb.Sp)和各向异性的程度(degree of anisotropy,DA)低于对照组。结论低剂量重组人甲状旁腺激素可提高大鼠骨折BV、BV/TV、Tb.Pf和BMD而降低Tb.Sp和DA,从而促进骨折的愈合。
文摘<strong>Background:</strong> <span style="font-family:""><span style="font-family:Verdana;">Cleft alveolus closure is a challenge for </span><span style="font-family:Verdana;">cleft</span> <span style="font-family:Verdana;">surgeon</span><span style="font-family:Verdana;"> for many decades. Secondary alveolar bone grafting (SABG) is the preferred technique using autologous cancellous </span><span style="font-family:Verdana;">ileac</span><span style="font-family:Verdana;"> crest bone as the donor graft. Many alternative methods were tried over the years with no</span></span><span style="font-family:Verdana;"> <span style="font-family:Verdana;">any</span></span><span style="font-family:""><span style="font-family:Verdana;"> promising results. Gingivoperiosteoplasty (GPP) is a good alternative surgical technique for bone regeneration in cleft alveolus with proper case selection criteria. </span><b><span style="font-family:Verdana;">Aim:</span></b><span style="font-family:Verdana;"> Aim of the study was to evaluate the bone regeneration following </span><span style="font-family:Verdana;">closure</span><span style="font-family:Verdana;"> of cleft alveolus with </span><span style="font-family:Verdana;">gingivoperiosteoplasty</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Method: </span></b><span style="font-family:Verdana;">This study was carried out in the department of Oral and Maxillofacial Surgery, Yenepoya Dental College, Deralakatte, Mangalore, Karnataka, India, from March 2018 to July 2019. In this </span><span style="font-family:Verdana;">study</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> we have performed </span><span style="font-family:Verdana;">gingivoperiosteoplasty</span><span style="font-family:Verdana;"> with palatoplasty and evaluated the amount of bone formation in the cleft alveolus using a series of </span><span style="font-family:Verdana;">intra oral</span><span style="font-family:Verdana;"> periapical radiographs. Five patients who required surgical repair of unilateral cleft palate and alveolus were selected for the study. </span><span style="font-family:Verdana;">Clinical</span><span style="font-family:Verdana;"> and radiographical assessment was done </span><span style="font-family:Verdana;">post operatively</span><span style="font-family:Verdana;">, after three and six months respectively for anatomical function and bone formation. </span><b><span style="font-family:Verdana;">Results:</span></b><span style="font-family:Verdana;"> After completion of clinical studies on patients</span></span><span style="font-family:Verdana;">,</span><span style="font-family:""><span style="font-family:Verdana;"> the statistical analysis of the data obtained. Radiographs were </span><span style="font-family:Verdana;">analy</span></span><span style="font-family:Verdana;">z</span><span style="font-family:Verdana;">ed</span><span style="font-family:""><span style="font-family:Verdana;"> for </span><span style="font-family:Verdana;">grey scale</span><span style="font-family:Verdana;"> density </span><span style="font-family:Verdana;">by means of</span><span style="font-family:Verdana;"> adobe </span><span style="font-family:Verdana;">photo shop</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">using</span><span style="font-family:""><span style="font-family:Verdana;"> the MATLAB process by histogram </span><span style="font-family:Verdana;">comparison</span></span><span style="font-family:""> </span><span style="font-family:Verdana;">in</span><span style="font-family:""><span style="font-family:Verdana;"> three months and six months, </span><a name="_Hlk58658647"></a><span style="font-family:Verdana;">showing denser </span><span style="font-family:Verdana;">grey scale</span><span style="font-family:Verdana;"> pattern, indicating the new bone formation in the cleft alveolus surgical site, in all the 5 surgical cases. </span><b><span style="font-family:Verdana;">Conclusion: </span></b><span style="font-family:Verdana;">Gingivoperiosteoplasty (GPP) is a good alternative procedure </span><span style="font-family:Verdana;">to</span><span style="font-family:Verdana;"> secondary alveolar bone grafting (SABG), so donor site morbidity can be avoided with reduced time and cost for the surgery. But a larger sample size and longer follow-up </span></span><span style="font-family:Verdana;">are</span><span style="font-family:""><span style="font-family:Verdana;"> necessary to understand the better reliability of this surgical technique. </span><b><span style="font-family:Verdana;">Clinical Significance: </span></b><span style="font-family:Verdana;">Gingivoperiosteoplasty in cleft alveolus patients showed significant bone formation in 3 to 6 months postoperatively.</span></span>
