Objective:To evaluate the effectiveness of DTwP-HB-Hib vaccination dosing intervals at eight weeks versus four weeks on the immunogenicity of the diphtheria component.Methods:This is a randomized,open-label,parallel,c...Objective:To evaluate the effectiveness of DTwP-HB-Hib vaccination dosing intervals at eight weeks versus four weeks on the immunogenicity of the diphtheria component.Methods:This is a randomized,open-label,parallel,controlled trial on healthy two-month-old infants who had not received DTwP-HB-Hib vaccinations.The infants received three doses of the vaccine either at an eight-week or four-week interval.The anti-diphtheria toxoid IgG antibody levels before and after three doses of the vaccination were measured using ELISA.Results:Eighty infants were enrolled in this study,with 64 fulfilling the study requirements and randomized into two groups.All study participants exhibited uncertain protection against diphtheria antibodies(0.01-0.99 IU/mL)at the beginning of the study with an average of(0.023±0.009)IU/mL and(0.026±0.009)IU/mL in eight weeks and four weeks groups.Following three doses of the vaccination,the antibody level rose to an average of(1.320±0.234)IU/mL in the eight-week group and(1.307±0.186)IU/mL in the four-week group,with no statistically significant difference in the antibody levels observed(P=0.814).Conclusions:DTwP-HB-Hib vaccinations can be administered at an eight-week or four-week interval,as they do not significantly affect the level of anti-diphtheria IgG antibodies.展开更多
目的探讨破伤风类毒素(Tetanus toxoid,TT)载体蛋白结合疫苗接种对儿童破伤风抗体水平的潜在影响。方法在山西省太原市开展回顾性观察队列研究,在既往按照国家免疫规划程序接种过4剂次百白破联合疫苗和1剂次白喉破伤风联合疫苗的11~13...目的探讨破伤风类毒素(Tetanus toxoid,TT)载体蛋白结合疫苗接种对儿童破伤风抗体水平的潜在影响。方法在山西省太原市开展回顾性观察队列研究,在既往按照国家免疫规划程序接种过4剂次百白破联合疫苗和1剂次白喉破伤风联合疫苗的11~13岁儿童中,选择仅接种过b型流感嗜血杆菌TT载体结合疫苗(Hemophilus influenzae type b TT-conjugated vaccine,Hib-TT)且无其他TT载体蛋白疫苗接种史儿童和无任何TT载体蛋白疫苗接种儿童作为观察对象,按照Hib-TT免疫史分为对照组(未接种过Hib-TT)、A组(仅在12月龄内接种3剂次Hib-TT)和B组(仅在12~18月龄接种1剂次Hib-TT),采用酶联免疫吸附试验检测血清破伤风IgG抗体浓度,比较A组和B组与对照组抗体几何平均浓度(Geometric mean concentration,GMC)、阳性率和达到保护性水平(IgG抗体浓度≥0.10 IU/mL)比例。结果对照组、A组、B组分别纳入146例、82例、136例,3组破伤风IgG抗体GMC分别为0.056 IU/mL、0.076 IU/mL、0.058 IU/mL,阳性率分别为95.38%、96.60%、94.34%,达到保护性水平比例分别为21.23%、42.68%、37.50%。A组、B组与对照组的抗体GMC比值分别为1.36(95%CI:1.09~1.72)、1.04(95%CI:0.86~1.51),抗体阳性率差值分别为1.22%(95%CI:-3.98%~6.41%)、-1.04%(95%CI:-6.21%~4.12%),抗体达到保护性水平比例差值分别为21.45%(95%CI:8.99%~33.94%)、16.27%(95%CI:5.69%~26.65%)。结论接种不同剂次Hib-TT可能对儿童破伤风保护性抗体水平产生影响。展开更多
为研制更有效的预防家畜D型产气荚膜梭菌肠毒血症的疫苗,本研究采用D型产气荚膜梭菌标准菌株增菌培养,经自制产毒培养基高效产毒,制备成D型产气荚膜梭菌氢氧化铝类毒素疫苗和白油类毒素疫苗。通过不同剂量疫苗免疫绵羊的攻毒保护试验和...为研制更有效的预防家畜D型产气荚膜梭菌肠毒血症的疫苗,本研究采用D型产气荚膜梭菌标准菌株增菌培养,经自制产毒培养基高效产毒,制备成D型产气荚膜梭菌氢氧化铝类毒素疫苗和白油类毒素疫苗。通过不同剂量疫苗免疫绵羊的攻毒保护试验和免疫绵羊的抗体水平监测,评估疫苗的免疫保护效果。结果表明:D型产气荚膜梭菌类毒素疫苗安全性良好,氢氧化铝苗和白油苗对绵羊有效免疫剂量均为4 m L(外毒素对小鼠半数致死量为2-6.4/m L),绵羊接种一个有效免疫剂量后,氢氧化铝疫苗免疫保护周期为6周以上;白油疫苗免疫保护周期为21周以上。本研究结果表明两种疫苗均具有良好的应用前景。展开更多
文摘Objective:To evaluate the effectiveness of DTwP-HB-Hib vaccination dosing intervals at eight weeks versus four weeks on the immunogenicity of the diphtheria component.Methods:This is a randomized,open-label,parallel,controlled trial on healthy two-month-old infants who had not received DTwP-HB-Hib vaccinations.The infants received three doses of the vaccine either at an eight-week or four-week interval.The anti-diphtheria toxoid IgG antibody levels before and after three doses of the vaccination were measured using ELISA.Results:Eighty infants were enrolled in this study,with 64 fulfilling the study requirements and randomized into two groups.All study participants exhibited uncertain protection against diphtheria antibodies(0.01-0.99 IU/mL)at the beginning of the study with an average of(0.023±0.009)IU/mL and(0.026±0.009)IU/mL in eight weeks and four weeks groups.Following three doses of the vaccination,the antibody level rose to an average of(1.320±0.234)IU/mL in the eight-week group and(1.307±0.186)IU/mL in the four-week group,with no statistically significant difference in the antibody levels observed(P=0.814).Conclusions:DTwP-HB-Hib vaccinations can be administered at an eight-week or four-week interval,as they do not significantly affect the level of anti-diphtheria IgG antibodies.
文摘目的探讨破伤风类毒素(Tetanus toxoid,TT)载体蛋白结合疫苗接种对儿童破伤风抗体水平的潜在影响。方法在山西省太原市开展回顾性观察队列研究,在既往按照国家免疫规划程序接种过4剂次百白破联合疫苗和1剂次白喉破伤风联合疫苗的11~13岁儿童中,选择仅接种过b型流感嗜血杆菌TT载体结合疫苗(Hemophilus influenzae type b TT-conjugated vaccine,Hib-TT)且无其他TT载体蛋白疫苗接种史儿童和无任何TT载体蛋白疫苗接种儿童作为观察对象,按照Hib-TT免疫史分为对照组(未接种过Hib-TT)、A组(仅在12月龄内接种3剂次Hib-TT)和B组(仅在12~18月龄接种1剂次Hib-TT),采用酶联免疫吸附试验检测血清破伤风IgG抗体浓度,比较A组和B组与对照组抗体几何平均浓度(Geometric mean concentration,GMC)、阳性率和达到保护性水平(IgG抗体浓度≥0.10 IU/mL)比例。结果对照组、A组、B组分别纳入146例、82例、136例,3组破伤风IgG抗体GMC分别为0.056 IU/mL、0.076 IU/mL、0.058 IU/mL,阳性率分别为95.38%、96.60%、94.34%,达到保护性水平比例分别为21.23%、42.68%、37.50%。A组、B组与对照组的抗体GMC比值分别为1.36(95%CI:1.09~1.72)、1.04(95%CI:0.86~1.51),抗体阳性率差值分别为1.22%(95%CI:-3.98%~6.41%)、-1.04%(95%CI:-6.21%~4.12%),抗体达到保护性水平比例差值分别为21.45%(95%CI:8.99%~33.94%)、16.27%(95%CI:5.69%~26.65%)。结论接种不同剂次Hib-TT可能对儿童破伤风保护性抗体水平产生影响。
文摘为研制更有效的预防家畜D型产气荚膜梭菌肠毒血症的疫苗,本研究采用D型产气荚膜梭菌标准菌株增菌培养,经自制产毒培养基高效产毒,制备成D型产气荚膜梭菌氢氧化铝类毒素疫苗和白油类毒素疫苗。通过不同剂量疫苗免疫绵羊的攻毒保护试验和免疫绵羊的抗体水平监测,评估疫苗的免疫保护效果。结果表明:D型产气荚膜梭菌类毒素疫苗安全性良好,氢氧化铝苗和白油苗对绵羊有效免疫剂量均为4 m L(外毒素对小鼠半数致死量为2-6.4/m L),绵羊接种一个有效免疫剂量后,氢氧化铝疫苗免疫保护周期为6周以上;白油疫苗免疫保护周期为21周以上。本研究结果表明两种疫苗均具有良好的应用前景。
