The toxicities of several fungicides to Pythium ultimum were determined by the method of mycelia growth. The results showed that hymexazol, Ovraclostrobin＋ Metiram, copper calcium sulphate, Thiophamate-merhyl and Fam...The toxicities of several fungicides to Pythium ultimum were determined by the method of mycelia growth. The results showed that hymexazol, Ovraclostrobin＋ Metiram, copper calcium sulphate, Thiophamate-merhyl and Famoxadone＋Cymoxanil all showed good inhibitory effects, of which Hymexazol was the best one with the ECho of 2.631 2 mg/L, followed by Ovractostrobin＋Metiram, whose ECso was 5.303 3 mg/L, and the effects of other fungicides were relatively poor. In the field fungicide screening test, the combination of 70% hymexazol wettable powder and 70% Thiophamate-merhyl had the control efficiency of 95.57%, which was better than the other test combinations, and it was applicable in the field control of tomato basal stem rot.展开更多
The continuous input of various emerging contaminants(ECs)has inevitably introduced large amounts of transformation products(TPs)in natural and engineering water scenarios.Structurally similar to the precursor species...The continuous input of various emerging contaminants(ECs)has inevitably introduced large amounts of transformation products(TPs)in natural and engineering water scenarios.Structurally similar to the precursor species,the TPs are expected to possess comparative,if not more serious,environmental properties and risks.This review summarizes the state-of-the-art knowledge regarding the integrated risk assessment frameworks of TPs of ECs,mainly involving the exposure-and effectdriven analysis.The inadequate information within existing frameworks that was essential and critical for developing a better risk assessment framework was discussed.The main strategic improvements include(1)non-targeted product analysis in both laboratory and field samples,(2)omics-based highthroughput toxicity assessment,(3)multichannel-driven mode of action in conjugation with effectdirected analysis,and(4)machine learning technology.Overall,this review provides a concise but comprehensive insight into the optimized strategy for evaluating the environmental risks and screening the key toxic products from the cocktail mixtures of ECs and their TPs in the global water cycle.This facilitates deciphering the mode of toxicity in complex chemical mixtures and prioritizing the regulated TPs among the unknown products,which have the potential to be considered a class of novel"ECs"ofgreatconcern.展开更多
Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial bloom.However,no synthetic cyanophage genome has been constructed and where its obstacles are unclear.Here,w...Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial bloom.However,no synthetic cyanophage genome has been constructed and where its obstacles are unclear.Here,we survey a stretch of 16 kb length sequence of cyanophage A-4L that is unclonable in Escherichia coli.We test 12 predicted promoters of cyanophage A-4L which were verified all active in E.coli.Next,we screen for eight ORFs that hindered the assembly of intermediate DNA fragments in E.coli and describe that seven ORFs in the 16 kb sequence could not be separately cloned in E.coli.All of unclonable ORFs in high-copy-number plasmid were successfully cloned using low-copy-number vector,suggesting that these ORFs were copy-number-dependent.We propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ORFs.Last,we de novo synthesized and assembled the full-length genome of cyanophage A-4L.This work deepens the understanding of synthetic cyanophages studies.展开更多
基金Supported by the Major agriculture application technology innovation project of Shandong Province"Establishment and demonstration of eco efficient and safe production technology model of protected vegetable"Major Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences(No.2014CXZ07)+3 种基金the Agricultural Science and Technology Innovation Project of Shandong Academy of Agricultural Sciences(CXGC2016B11,CXGC2016A09)Special Fund for Agro-scientific Research in the Public Interest(201503112)the Major Project of National Agricultural Product Quality Safety Risk Assessment(GJFP2016013)~~
文摘The toxicities of several fungicides to Pythium ultimum were determined by the method of mycelia growth. The results showed that hymexazol, Ovraclostrobin＋ Metiram, copper calcium sulphate, Thiophamate-merhyl and Famoxadone＋Cymoxanil all showed good inhibitory effects, of which Hymexazol was the best one with the ECho of 2.631 2 mg/L, followed by Ovractostrobin＋Metiram, whose ECso was 5.303 3 mg/L, and the effects of other fungicides were relatively poor. In the field fungicide screening test, the combination of 70% hymexazol wettable powder and 70% Thiophamate-merhyl had the control efficiency of 95.57%, which was better than the other test combinations, and it was applicable in the field control of tomato basal stem rot.
基金the Natural Science Foundation of China-Joint Fund Project(No.U2005206)the Xiamen Municipal Bureau of Science and Technology(No.YDZX20203502000003)the support of the President Research Funds from Xiamen University(No.20720210081).
文摘The continuous input of various emerging contaminants(ECs)has inevitably introduced large amounts of transformation products(TPs)in natural and engineering water scenarios.Structurally similar to the precursor species,the TPs are expected to possess comparative,if not more serious,environmental properties and risks.This review summarizes the state-of-the-art knowledge regarding the integrated risk assessment frameworks of TPs of ECs,mainly involving the exposure-and effectdriven analysis.The inadequate information within existing frameworks that was essential and critical for developing a better risk assessment framework was discussed.The main strategic improvements include(1)non-targeted product analysis in both laboratory and field samples,(2)omics-based highthroughput toxicity assessment,(3)multichannel-driven mode of action in conjugation with effectdirected analysis,and(4)machine learning technology.Overall,this review provides a concise but comprehensive insight into the optimized strategy for evaluating the environmental risks and screening the key toxic products from the cocktail mixtures of ECs and their TPs in the global water cycle.This facilitates deciphering the mode of toxicity in complex chemical mixtures and prioritizing the regulated TPs among the unknown products,which have the potential to be considered a class of novel"ECs"ofgreatconcern.
基金supported by the grants from the National Key Research and Development Program of China (No.2018YFA0903000)the National Natural Science Foundation of China (31901019)and the China Postdoctoral Science foundation (2021M692389).
文摘Artificial cyanophages are considered to be an effective biological method to control harmful cyanobacterial bloom.However,no synthetic cyanophage genome has been constructed and where its obstacles are unclear.Here,we survey a stretch of 16 kb length sequence of cyanophage A-4L that is unclonable in Escherichia coli.We test 12 predicted promoters of cyanophage A-4L which were verified all active in E.coli.Next,we screen for eight ORFs that hindered the assembly of intermediate DNA fragments in E.coli and describe that seven ORFs in the 16 kb sequence could not be separately cloned in E.coli.All of unclonable ORFs in high-copy-number plasmid were successfully cloned using low-copy-number vector,suggesting that these ORFs were copy-number-dependent.We propose a clone strategy abandoned the promotor and the start codon that could be applied for unclonable ORFs.Last,we de novo synthesized and assembled the full-length genome of cyanophage A-4L.This work deepens the understanding of synthetic cyanophages studies.
