The paper presents the investigation of the possibility of hepatic tissue restoration by the platelet-rich plasma(PRP)in case of toxic hepatitis.It is shown PRP injection into liver reduced an amount of connective tis...The paper presents the investigation of the possibility of hepatic tissue restoration by the platelet-rich plasma(PRP)in case of toxic hepatitis.It is shown PRP injection into liver reduced an amount of connective tissue and collagen fibers at the places of hepatocytes necrosis after experimental toxic influence.PRP administration leads to rapid regeneration of hepatic tissues with restoration of its normal structure.As PRP is concentrate of autologous blood cells,it doesn't cause any side effects and doesn't require immunosuppressive therapy.展开更多
Constructing of heterojunction was identified as a feasible way to improve photocatalytic activity of pho-tocatalyst.In this work,a n-p type Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction was successfully prepared for ...Constructing of heterojunction was identified as a feasible way to improve photocatalytic activity of pho-tocatalyst.In this work,a n-p type Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction was successfully prepared for organic pollutants degradation.This Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction exhibited much higher pho-tocatalytic activity towards Rhodamine B(92.24%,expose to visible light for 60 min),norfloxacin(81.73%,expose to visible light for 90 min)and levofloxacin(87.46%,expose to visible light for 90 min)than pure Bi_(2)WO_(6)and pure AgInS_(2).Toxicity analysis indicated the low environmental toxicity of Rhodamine B degradation intermediates for Rye seeds and Sudangrass seeds germination and growth.Mechanism study displayed that AgInS_(2)and Bi_(2)WO_(6)work as the primary photocatalyst to form·O_(2)−and ·OH,respectively.The improved photocatalytic activity of the Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction was due to the im-proved light response range and intensified carrier separation capability.Additionally,a S-scheme charge transfer mechanism including multiple charge transfer channels was proposed.This work could provide an effective strategy for organic pollutants degradation in wastewater.展开更多
[目的]将网络药理学与分子对接技术融入实验教学设计与实践,帮助本科生构建从分子水平到整体疗效的学习路径,深化对方剂机制的理解。[方法]以四逆汤组方配伍减毒增效为例,通过中药系统药理学数据库与分析平台(Traditional Chinese Medic...[目的]将网络药理学与分子对接技术融入实验教学设计与实践,帮助本科生构建从分子水平到整体疗效的学习路径,深化对方剂机制的理解。[方法]以四逆汤组方配伍减毒增效为例,通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、Cytoscape 3.9.0软件构建网络,运用基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析、分子对接等手段开展教学,并在教学中以实验预习、实验模拟以及团队协作能力培养为抓手,引导学生自主设计并完善验证性实验,最终依据线上操作和线下实验报告考核,完成过程和结果评价,及时做好教学反馈。[结果]通过收集四逆汤118个活性成分与364个共有靶点,学生可作图分析出丝氨酸/苏氨酸蛋白激酶1(serine/threonine-protein kinase 1,AKT1)和白细胞介素-6(interleukin-6,IL-6)等核心靶点与相应信号通路,并经分子对接获得可视化数据。与传统教学相比,实验教学班级期末得分率较高,学习成果显著。[结论]网络药理学与分子对接技术在方剂学课程中的应用,有助于学生理解方剂的作用机制及疗效,有效提升方剂学的教学质量。展开更多
Chemoprevention is one of the cancer prevention approaches wherein natural/synthetic agent(s) are prescribed with the aim to delay or disrupt multiple pathways and processes involved at multiple steps, i.e., initiatio...Chemoprevention is one of the cancer prevention approaches wherein natural/synthetic agent(s) are prescribed with the aim to delay or disrupt multiple pathways and processes involved at multiple steps, i.e., initiation, promotion, and progression of cancer. Amongst environmental chemopreventive compounds, diet/beverage-derived components are under evaluation, because of their long history of exposure to humans, high tolerability, low toxicity, and reported biological activities. This compilation briefly covers and compares the available evidence on chemopreventive efficacy and probable mechanism of chemoprevention by selected dietary phytochemicals(capsaicin, curcumin, diallyl sulphide, genistein, green/black tea polyphenols, indoles, lycopene, phenethyl isocyanate, resveratrol, retinoids and tocopherols) in experimental systems and clinical trials. All the dietary phytochemicals covered in this review have demonstrated chemopreventive efficacy against spontaneous or carcinogen-induced experimental tumors and/or associated biomarkers and processes in rodents at several organ sites. The observed anti-initiating, anti-promoting and anti-progression activity of dietary phytochemicals in carcinogen-induced experimental models involve phytochemical-mediated redox changes, modulation of enzymes and signaling kinases resulting to effects on multiple genes and cell signaling pathways. Results from clinical trials using these compounds have not shown them to be chemopreventive. This may be due to our:(1) inability to reproduce the exposure conditions, i.e., levels, complexity, other host and lifestyle factors; and(2) lack of understanding about the mechanisms of action and agent-mediated toxicity in several organs and physiological processes in the host. Current research efforts in addressing the issues of exposure conditions, bioavailability, toxicity and the mode of action of dietary phytochemicals may help address the reason for observed mismatch that may ultimately lead to identification of new chemopreventive agents for protection against broad spectrum of exposures.展开更多
文摘The paper presents the investigation of the possibility of hepatic tissue restoration by the platelet-rich plasma(PRP)in case of toxic hepatitis.It is shown PRP injection into liver reduced an amount of connective tissue and collagen fibers at the places of hepatocytes necrosis after experimental toxic influence.PRP administration leads to rapid regeneration of hepatic tissues with restoration of its normal structure.As PRP is concentrate of autologous blood cells,it doesn't cause any side effects and doesn't require immunosuppressive therapy.
基金supported by the Specialized Research Fund of Education Department of Shaanxi Province(No.22JY015)the College Students Innovation and Entrepreneurship Plan Training Program(No.S202211396006).
文摘Constructing of heterojunction was identified as a feasible way to improve photocatalytic activity of pho-tocatalyst.In this work,a n-p type Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction was successfully prepared for organic pollutants degradation.This Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction exhibited much higher pho-tocatalytic activity towards Rhodamine B(92.24%,expose to visible light for 60 min),norfloxacin(81.73%,expose to visible light for 90 min)and levofloxacin(87.46%,expose to visible light for 90 min)than pure Bi_(2)WO_(6)and pure AgInS_(2).Toxicity analysis indicated the low environmental toxicity of Rhodamine B degradation intermediates for Rye seeds and Sudangrass seeds germination and growth.Mechanism study displayed that AgInS_(2)and Bi_(2)WO_(6)work as the primary photocatalyst to form·O_(2)−and ·OH,respectively.The improved photocatalytic activity of the Bi_(2)WO_(6)/AgInS_(2)S-scheme heterojunction was due to the im-proved light response range and intensified carrier separation capability.Additionally,a S-scheme charge transfer mechanism including multiple charge transfer channels was proposed.This work could provide an effective strategy for organic pollutants degradation in wastewater.
文摘[目的]将网络药理学与分子对接技术融入实验教学设计与实践,帮助本科生构建从分子水平到整体疗效的学习路径,深化对方剂机制的理解。[方法]以四逆汤组方配伍减毒增效为例,通过中药系统药理学数据库与分析平台(Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,TCMSP)、Cytoscape 3.9.0软件构建网络,运用基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析、分子对接等手段开展教学,并在教学中以实验预习、实验模拟以及团队协作能力培养为抓手,引导学生自主设计并完善验证性实验,最终依据线上操作和线下实验报告考核,完成过程和结果评价,及时做好教学反馈。[结果]通过收集四逆汤118个活性成分与364个共有靶点,学生可作图分析出丝氨酸/苏氨酸蛋白激酶1(serine/threonine-protein kinase 1,AKT1)和白细胞介素-6(interleukin-6,IL-6)等核心靶点与相应信号通路,并经分子对接获得可视化数据。与传统教学相比,实验教学班级期末得分率较高,学习成果显著。[结论]网络药理学与分子对接技术在方剂学课程中的应用,有助于学生理解方剂的作用机制及疗效,有效提升方剂学的教学质量。
文摘Chemoprevention is one of the cancer prevention approaches wherein natural/synthetic agent(s) are prescribed with the aim to delay or disrupt multiple pathways and processes involved at multiple steps, i.e., initiation, promotion, and progression of cancer. Amongst environmental chemopreventive compounds, diet/beverage-derived components are under evaluation, because of their long history of exposure to humans, high tolerability, low toxicity, and reported biological activities. This compilation briefly covers and compares the available evidence on chemopreventive efficacy and probable mechanism of chemoprevention by selected dietary phytochemicals(capsaicin, curcumin, diallyl sulphide, genistein, green/black tea polyphenols, indoles, lycopene, phenethyl isocyanate, resveratrol, retinoids and tocopherols) in experimental systems and clinical trials. All the dietary phytochemicals covered in this review have demonstrated chemopreventive efficacy against spontaneous or carcinogen-induced experimental tumors and/or associated biomarkers and processes in rodents at several organ sites. The observed anti-initiating, anti-promoting and anti-progression activity of dietary phytochemicals in carcinogen-induced experimental models involve phytochemical-mediated redox changes, modulation of enzymes and signaling kinases resulting to effects on multiple genes and cell signaling pathways. Results from clinical trials using these compounds have not shown them to be chemopreventive. This may be due to our:(1) inability to reproduce the exposure conditions, i.e., levels, complexity, other host and lifestyle factors; and(2) lack of understanding about the mechanisms of action and agent-mediated toxicity in several organs and physiological processes in the host. Current research efforts in addressing the issues of exposure conditions, bioavailability, toxicity and the mode of action of dietary phytochemicals may help address the reason for observed mismatch that may ultimately lead to identification of new chemopreventive agents for protection against broad spectrum of exposures.
