Oxidative stress(OS)is detri me ntal to sperm fun ctio ns,and the oxidation reductio n pote ntial(ORP)is a good measure of OS as it considers the balance betwee n oxidants and reducta nts.Total motile sperm count(TMSC...Oxidative stress(OS)is detri me ntal to sperm fun ctio ns,and the oxidation reductio n pote ntial(ORP)is a good measure of OS as it considers the balance betwee n oxidants and reducta nts.Total motile sperm count(TMSC)is viewed as the single most im porta nt semen analysis parameter that can predict male infertility severity,and its correlation with ORP has never been undertaken.The objectives of this study were to assess the correlation between ORP and TMSC,to identify the ORP cutoff value based on the TMSC result,and to compare this cutoff value with previously reported ORP cutoff values in literature.One thousand one hundred and sixty-eight infertile patients and 100 fertile controls were enrolled.Demographic and semen data of the participants were retrieved and analyzed.Wilcox on's rank-sum test compared variables betwee n infertile men and fertile controls;Spearman's correlation assessed the static ORP(sORP)-TMSC relationship for the whole sample and among each group individually.Using a 20×10^6TMSC threshold,receiver operator characteristic(ROC)analysis determined the sORP cutoff associated with the highest predictive values.TMSC was significantly negatively correlated with sORP across all participants(r=0.86,P<0.001),among infertile patients(r=0.729,P<0.001),and among fertile controls(r=0.53,P<0.001).A 20-million TMSC threshold determined an sORP cutoff value of 2.34 mV/106sperm/ml to be associated with 82.9%sensitivity,82.8%specificity,91.5%positive predictive value(PPV),68.5%negative predictive value(NPV),and 82.9%overall accuracy.Compared with previously reported cutoff values in searched literature,the 2.34 mV/10^6sperm/ml cutoff value identified in our study yielded the highest overall diagnostic accuracy in the evaluation of in fertile men.展开更多
Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of...Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">test diet. Two additional levels providing 2.38 and 2.94</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> no treatment</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) and total sperm cells per ejaculate increased from 69.7 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> to 82.0 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.展开更多
Varicocele is a prevalent condition in the infertile male population.However,to date,which patients may benefit most from varicocele repair is still a matter of debate.The purpose of this study was to evaluate whether...Varicocele is a prevalent condition in the infertile male population.However,to date,which patients may benefit most from varicocele repair is still a matter of debate.The purpose of this study was to evaluate whether certain preintervention sperm parameters are predictive of successful varicocele repair,defined as an improvement in total motile sperm count(TMSC).We performed a retrospective study on 111 patients with varicocele who had undergone varicocele repair,collected from the Department of Endocrinology,Metabolic Diseases and Nutrition,University of Catania(Catania,Italy),and the Unit of Urology at the Selcuk University School of Medicine(Konya,Türkiye).The predictive analysis was conducted through the use of the Brain Project,an innovative tool that allows a complete and totally unbiased search of mathematical expressions that relate the object of study to the various parameters available.Varicocele repair was considered successful when TMSC increased by at least 50% of the preintervention value.For patients with preintervention TMSC below 5×10^(6),improvement was considered clinically relevant when the increase exceeded 50% and the absolute TMSC value was>5×10^(6).From the preintervention TMSC alone,we found a model that predicts patients who appear to benefit little from varicocele repair with a sensitivity of 50.0% and a specificity of 81.8%.Varicocele grade and serum follicle-stimulating hormone(FSH)levels did not play a predictive role,but it should be noted that all patients enrolled in this study were selected with intermediate-or high-grade varicocele and normal FSH levels.In conclusion,preintervention TMSC is predictive of the success of varicocele repair in terms of TMSC improvement in patients with intermediate-or high-grade varicoceles and normal FSH levels.展开更多
This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 a...This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.展开更多
文摘Oxidative stress(OS)is detri me ntal to sperm fun ctio ns,and the oxidation reductio n pote ntial(ORP)is a good measure of OS as it considers the balance betwee n oxidants and reducta nts.Total motile sperm count(TMSC)is viewed as the single most im porta nt semen analysis parameter that can predict male infertility severity,and its correlation with ORP has never been undertaken.The objectives of this study were to assess the correlation between ORP and TMSC,to identify the ORP cutoff value based on the TMSC result,and to compare this cutoff value with previously reported ORP cutoff values in literature.One thousand one hundred and sixty-eight infertile patients and 100 fertile controls were enrolled.Demographic and semen data of the participants were retrieved and analyzed.Wilcox on's rank-sum test compared variables betwee n infertile men and fertile controls;Spearman's correlation assessed the static ORP(sORP)-TMSC relationship for the whole sample and among each group individually.Using a 20×10^6TMSC threshold,receiver operator characteristic(ROC)analysis determined the sORP cutoff associated with the highest predictive values.TMSC was significantly negatively correlated with sORP across all participants(r=0.86,P<0.001),among infertile patients(r=0.729,P<0.001),and among fertile controls(r=0.53,P<0.001).A 20-million TMSC threshold determined an sORP cutoff value of 2.34 mV/106sperm/ml to be associated with 82.9%sensitivity,82.8%specificity,91.5%positive predictive value(PPV),68.5%negative predictive value(NPV),and 82.9%overall accuracy.Compared with previously reported cutoff values in searched literature,the 2.34 mV/10^6sperm/ml cutoff value identified in our study yielded the highest overall diagnostic accuracy in the evaluation of in fertile men.
文摘Research findings for supplementing boar stud diets with fish oils are inconsistent. This study was designed to address three possible causes of performance variation of boars to fish oil supplementation: stability of the fatty acid source, level of inclusion and breed of boars tested. Three groups of 87 boars each, from two genetic lines (PIC 337 and PIC 800), were assigned to treatment based on age, mean sperm production (previous 12 weeks), and body condition score. All boars received a corn-soybean meal diet with a commercial fish oil supplement providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/boar/day of docosahexaenoic acid (DHA) as a preconditioning diet. On 10-Aug., 2020, the DHA source was changed to a stabilized starch imbedded source of refined fish oil (Salmate®), providing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d for </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">test diet. Two additional levels providing 2.38 and 2.94</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA were fed for a 9 week pretreatment period and during the test period. Salmate® fed at 2.38</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d of DHA resulted in a reduction in the number of rejected ejaculates (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.045) by 7.5% and 6.4% compared to the lowest and highest inclusion rates, respectively. There w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> no treatment</span><span style="font-family:Verdana;">s</span><span style="font-family:Verdana;"> by genetic line interactions. A retrospective study of semen production and quality of 77 boars on the Salmate® diet containing 1.83</span><span style="font-family:""> </span><span style="font-family:Verdana;">g/b/d DHA was done to compare to the original source of DHA at the same inclusion level. There were no differences in semen quality parameters between the 2 lipid sources. Ejaculate volume increased from 177.9 ml to 233.4 ml (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) and total sperm cells per ejaculate increased from 69.7 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> to 82.0 </span><span style="font-family:""><span style="font-family:Verdana;">×</span><span style="font-family:Verdana;"> 10<sup></span></span><span style="font-family:Verdana;">9</span><span style="font-family:Verdana;"></sup> (P</span><span style="font-family:""> </span><span style="font-family:Verdana;"><</span><span style="font-family:""> </span><span style="font-family:Verdana;">0.001) due to substitution of Salmate®. Adding Salmate® at 2.38 g/b/d resulted in a lower number of rejected ejaculates per boar by 7.5% and 6.4% vs. 1.83 and 2.94 g/b/d, respectively, and boars fed Salmate® at 1.83 g/b/d produced 17% more doses than the competing product.
文摘Varicocele is a prevalent condition in the infertile male population.However,to date,which patients may benefit most from varicocele repair is still a matter of debate.The purpose of this study was to evaluate whether certain preintervention sperm parameters are predictive of successful varicocele repair,defined as an improvement in total motile sperm count(TMSC).We performed a retrospective study on 111 patients with varicocele who had undergone varicocele repair,collected from the Department of Endocrinology,Metabolic Diseases and Nutrition,University of Catania(Catania,Italy),and the Unit of Urology at the Selcuk University School of Medicine(Konya,Türkiye).The predictive analysis was conducted through the use of the Brain Project,an innovative tool that allows a complete and totally unbiased search of mathematical expressions that relate the object of study to the various parameters available.Varicocele repair was considered successful when TMSC increased by at least 50% of the preintervention value.For patients with preintervention TMSC below 5×10^(6),improvement was considered clinically relevant when the increase exceeded 50% and the absolute TMSC value was>5×10^(6).From the preintervention TMSC alone,we found a model that predicts patients who appear to benefit little from varicocele repair with a sensitivity of 50.0% and a specificity of 81.8%.Varicocele grade and serum follicle-stimulating hormone(FSH)levels did not play a predictive role,but it should be noted that all patients enrolled in this study were selected with intermediate-or high-grade varicocele and normal FSH levels.In conclusion,preintervention TMSC is predictive of the success of varicocele repair in terms of TMSC improvement in patients with intermediate-or high-grade varicoceles and normal FSH levels.
文摘This study evaluated the relationship between serum L-carnitine level and sperm parameters in young boars. Serum L-carnitine and semen characteristics were determined for 61 young Duroc boars between the ages of 590 and 630 days. Multiple linear regression analysis was performed to predict total and progressive motility and the total number of spermatozoa based on serum total L-carnitine and free L-carnitine levels. Total number of spermatozoa was not associated with basal serum L-carnitine levels. A regression equation was found in which both total L-carnitine levels and free L-carnitine levels were significant predictors of total and progressive motility (P 0.05). These results suggest that serum L-carnitine level is an important selection parameter for stock boars.
