[Objective] The effects of wheat based diet supplemented with xylanase on blood sugar and total protein in serum of geese were studied. [ Method ] By using the randomized design of single factor, the 1-day-old healthy...[Objective] The effects of wheat based diet supplemented with xylanase on blood sugar and total protein in serum of geese were studied. [ Method ] By using the randomized design of single factor, the 1-day-old healthy goslings were divided into 6 groups and fed with corn based diet, wheat based diet and wheat based diet supplemented with xylanase at different concentrations respectively, the contents of blood sugar and total protein in serum were determined. [ Result] The wheat based diet supplemented with xylanase could increase the blood sugar and total protein in serum of geese and wheat based diet supplemented with 0.2% xylanase generated the best effect, which was higher than those of corn based diet group. As for the concentration of protein in serum, wheat based diet supplemented with 0.2% xylanase was significantly different from corn based diet and wheat based diet. [ Conclusion] The wheat based diet supplemented with xylanase could enhance geese production.展开更多
Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the tota...Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the total protein and albumin were found to vary linearly with concentration. Hence by calculating the nonlinear refractive index it is possible to measure their concentration in the sample. The values measured thus are found in equivalence with conventional colorimetric method.展开更多
Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ ...Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.展开更多
The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress an...The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.展开更多
Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of ex...Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.展开更多
Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods...Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods Primary cultured hippocampal neurons, the cultures were pretreated with tSDA and GRb1 on 10d for 24 hours respectively. Then the cultures were treated with 35 μmol·L -1 Aβ25-35 for 24 hours, observed the changing of survival rate of neurons and the apoptosis of neurons with biochemical analysis combining immunofluorescent cytochemical double-staining technique. Results Hippocampal neurons were treated with 35 μmol·L -1 Aβ for 24 hours, and survival rate of neurons downed to 52.6%. When neurons were pretreated by tSDA and GRb1, survival rate of neurons increased 11% to 15%. The findings of immunofluorescent cytochemical double-staining indicated that apoptotic neurons were obviously more than that of the blank group, reaching 43.9%.When neurons were pretreated by tSDA and GRb1, apoptotic neurons were downed to 16.6%, 10.8% respectively. Conclusion tSDA had the same effects as GRb1, protecting the neurons, antagonizing neurotoxicity of Aβ, increasing survival rate of neurons, and reducing apoptotic neurons induced by Aβ.展开更多
[Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fer...[Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fertility, different amounts of N-fertilizer were applied in the plots, and then the contents of total protein and its components, percentage of the component content to total protein content as well as the processing quality of grains of two strong-gluten wheat varieties （Linyou145 and Zhengmai9023） and two weak-gluten wheat varieties （Ningmai9 and Baofeng949） were determined. [Result] The contents of total protein and globulin, gliadin and glutenin were improved significantly with the increase of the N-fertilizer amount; but the content of albumin did not show remarkable increase; in addition, the percentage of each protein component was relative stable and did not increase significantly. Increase in the amount of N-fertilizer improved the sedimentation value, wet gluten content, loaf volume and loaf score, decreased the volume weight of grain. [Conclusion] This study provideed theoretical support for high-quality wheat production.展开更多
Background and Aim: Despite the fact that acute appendicitis is the most common surgical emergency all around the world, its diagnosis is still based on clinical evaluation and accuracy of the diagnosis depending on e...Background and Aim: Despite the fact that acute appendicitis is the most common surgical emergency all around the world, its diagnosis is still based on clinical evaluation and accuracy of the diagnosis depending on experience. The aim of this study is to evaluate the role of inflammatory markers in diagnosis of acute appendicitis. Material and Method: The study includes 77 cases with histopathologically proven acute appendicitis and 17 control cases. Blood samples were obtained from all cases and C-reactive protein (CRP), Granulocyte Colony Stimulating Factor (G-CSF) and Total Antioxidant Capacity (TAC) were measured. Findings: In cases with acute appendicitis, CRP and G-CSF levels were found to be related to acute appendicitis;however, TAC was not affected by the disease process. Moreover, CRP and G-CSF levels were correlated with the disease severity. Conclusion: Both CRP and G-CSF can be used in diagnosis of acute appendicitis. Furthermore, increased CRP level can be a marker to show advanced cases. However, G-CSF is not an effective marker to show disease severity.展开更多
A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an...A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%.展开更多
Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content ...Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content and protein expression pattern to assess the conservation status ofPosidoniaplants from the S. Marinella (Rome, Italy) meadow. The total phenol content was inversely related to maximum beddensity, confirming the relationship betweenhigh phenol content and stressful conditions. In addition, protein expression pattern profilesshowed that the number of differentially expressed proteins was dramatically reduced in the latest years compared to previous analyses. Our results support the usefulness of integrating solid descriptors, such as phenol content, with novel biochemical/molecular approaches in the monitoring of meadows.展开更多
The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with ...The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with CpTI gene were determined in order to study the products of CpTI gene expression at protein level. The results indicated that the amount of total soluble protein was greater in transgenic poplars than in non transgenic poplars, but was more in the metaphylla than in browse. The expression of CpTI gene resulted in an obvious increase in CpTI content, whereas CpTI was not detected in non transgenic poplars. It was found that there were high amount of total soluble protein and CpTI in 3 clones of TG07, TG04 and TG71 compared with other transgenic clones. In addition, the analysis of protein by SDS PAGE showed that a specific protein band of about 11.3?kD corresponding to the 80 amino acids encoded by the CpTI gene was observed in transgenic poplars on the gel of protein, which was not detected in non transgenic poplars.展开更多
为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和...为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和挥发性盐基氮(Total volatile base nitrogen,TVB-N)含量的近红外定量分析模型。采集近红外光谱后,通过比较决定系数(Coefficient of determination,R^(2))、校正标准偏差(Root mean square error of calibration,RMSEC)、预测标准偏差(Root mean square error of prediction,RMSEP)等模型的评价参数,选取近红外光谱定量分析模型的最佳预处理方式、特征光谱范围以及主因子数。结果显示,NPN含量模型的最佳预处理方法为多元散射校正(Multiplicative signal correction,MSC),其特征光谱范围为8887.1~7774.2 cm-1;TVB-N含量模型则采用MSC与卷积平滑(Savitzky-Golay smoothing,SG)相结合的方式进行预处理,建模范围为全波段。两个定量模型的主因子数均为5。经模型的优化与外部验证,最终构建的PLS最优模型如下:NPN含量近红外定量分析模型的R^(2)为0.9384,RMSEC为0.279,RMSEP为0.443;TVB-N含量近红外定量分析模型的R^(2)为0.8685,RMSEC为3.800,RMSEP为4.070。研究结果表明,两个模型均具有良好的预测精度(R^(2)>0.85)和稳定性,其中NPN定量分析模型的预测能力优于TVB-N定量分析模型。综上,本研究基于NIRS与PLS构建的定量分析模型能够有效预测南极磷虾体内的NPN和TVB-N含量,为南极磷虾的品质评价提供了可靠的技术支持,满足快速评定的实际应用需求。展开更多
Objectives: To establish an ADL prediction model for Parkinson’s inpatients as an auxiliary evaluation scheme. Methods: The data of Parkinson’s patients hospitalized in the Department of Neurology of Affiliated Brai...Objectives: To establish an ADL prediction model for Parkinson’s inpatients as an auxiliary evaluation scheme. Methods: The data of Parkinson’s patients hospitalized in the Department of Neurology of Affiliated Brain Hospital of Guangzhou Medical University from 2019 to 2022, which suited the criteria were collected, and a multiple linear regression model was established with serum total protein, serum albumin, age, BMI and education level as independent variables and BI scores as dependent variables. Results: A total of 95 PD patients were included (mean 70.05 ± 10.87 years): 53 males and 42 females. The correlation analysis showed that the serum total protein (r = 0.398, P Conclusion: The ADL multiple linear regression model can be used as an important means to evaluate the ADL ability of PD patients in hospital.展开更多
BACKGROUND： The differential diagnosis of many neurodegenerative disorders depends primarily on clinical symptoms together with imaging methods. Recently, increased importance has been placed on the use of biomarkers...BACKGROUND： The differential diagnosis of many neurodegenerative disorders depends primarily on clinical symptoms together with imaging methods. Recently, increased importance has been placed on the use of biomarkers for diagnosing various neurodegenerative disorders. OBJECTIVE： To assess the feasibility of tau-protein, phosphorylated tau-protein, beta-amyloid 42 （Aβ42）, and 14-3-3 protein as biomarkers for diagnosing several neurodegenerative diseases complicated by cognitive deficits. DESIGN, TIME AND SETTING： A non-randomized, concurrent, case-control investigation was performed in three medical centers in the Czech Republic （Department of Neurology at the University Hospital in Hradec Kralove, Department of Neurology at the 2rd Medical Faculty, and the University Hospital Motol） between October 2000 and November 2006. PARTICIPANTS： Eighteen patients with probable AIzheimer＇s disease, 4 patients with Creutzfeldt-Jakob disease, 10 patients with frontotemporal dementia, 9 patients with clinically isolated syndrome suggestive of multiple sclerosis, and 7 patients with multiple sclerosis, as well as 38 race-, nationality-, and age-matched cognitively intact controls, were included in the study. Diagnoses were established based on the following criteria： the criteria for Alzheimer＇s disease proposed by the National Institute of Neurological and Communicative Disorders and Stroke/Alzheimer＇s Disease and Related Disorders Association, WHO criteria for Creutzfeldt-Jakob disease, Neary criteria for frontotemporal dementia, and McDonald＇s criteria for multiple sclerosis. All included patients were confirmed to suffer from various degrees of dementia. METHODS： Enzyme-linked immunosorbent assay was used to measure concentrations of tau-protein, phosphorylated tau-protein, and Aβ42 in cerebrospinal fluid （CSF） samples collected by standard lumbar puncture from each patient. Moreover, 14-3-3 protein was assessed by Western blot in CSF of Creutzfeldt-Jakob disease patients. Cognitive status was assessed using the Mini Mental Scale Examination （MMSE） in all subjects. MAIN OUTCOME MEASURES： Establishment of biomarkers with greatest specificity and sensitivity for the investigated disorders according to Receiver Operating Characteristic curves, which were based on values from patients and controls; correlation between concentrations of given biomarkers and demographic parameters, diagnosis, duration of disease, and level of cognitive deficit. RESULTS： Increased concentrations of total tau protein and phosphorylated tau protein, and decreased levels of Aβ42, in CSF of Alzheimer＇s disease patients reached the required sensitivity/specificity ratio of 80% or greater. A marked elevation in CSF concentrations of total tau protein showed even greater sensitivity than 14-3-3 protein in Creutzfeldt-Jakob disease. There was no association between selected biomarkers and frontotemporal dementia or multiple sclerosis. Phosphorylated tau-protein was the only biomarker that noticeably correlated with MMSE scores for Alzheimer＇s disease.CONCLUSION： Levels of total tau protein, phosphorylated tau protein, and A!342 in the CSF could differentiate patients with Alzheimer＇s disease and Creutzfeldt-Jakob disease from healthy controls and patients with other neurodegenerative disorders. The diversity of absolute values demonstrates the necessity to establish a specific standard for each laboratory.展开更多
基金the Science and Technology Key Projects of China (2004BA514A13-9)~~
文摘[Objective] The effects of wheat based diet supplemented with xylanase on blood sugar and total protein in serum of geese were studied. [ Method ] By using the randomized design of single factor, the 1-day-old healthy goslings were divided into 6 groups and fed with corn based diet, wheat based diet and wheat based diet supplemented with xylanase at different concentrations respectively, the contents of blood sugar and total protein in serum were determined. [ Result] The wheat based diet supplemented with xylanase could increase the blood sugar and total protein in serum of geese and wheat based diet supplemented with 0.2% xylanase generated the best effect, which was higher than those of corn based diet group. As for the concentration of protein in serum, wheat based diet supplemented with 0.2% xylanase was significantly different from corn based diet and wheat based diet. [ Conclusion] The wheat based diet supplemented with xylanase could enhance geese production.
文摘Z-scan technique is an effective tool for measuring the optical nonlinearity of the materials. By using this technique the measurement was made for total protein and albumin. The nonlinear refractive index of the total protein and albumin were found to vary linearly with concentration. Hence by calculating the nonlinear refractive index it is possible to measure their concentration in the sample. The values measured thus are found in equivalence with conventional colorimetric method.
文摘Populus tomentosa seedlings used for cold acclimation at -3℃ were pretreated with or without 10?mmol·L -1 CaCl 2, 3?mmol·L -1 of Ca 2+ chelator EGTA, 0 1?mmol·L -1 of Ca 2+ channel inhibitor LaCl 3,and 0 05?mmol·L -1 of CaM antagonist CPZ. The changes in the contents of total soluble protein and CaM, and freezing resistance in all pretreated seedlings in various periods ( viz: following cold acclimation, chilling stress and recovery) were investigated. Results showed that cold acclimation increased the contents of total soluble protein and CaM, and freezing resistance of seedlings, which could be strongly reduced by the pretreatments of EGTA CPZ and LaCl 3 Cold acclimation combined with CaCl 2 pretreatment enhanced the effect of cold acclimation on freezing resistance, and obviously increased the contents of total soluble protein and CaM, reduced the declining degree of the contents of total soluble protein and CaM caused by chilling stress as compared with cold acclimation, augmented the increase in the level of total soluble protein and CaM during the recovery periods. Further analysis found that an increase in total soluble protein content during cold acclimation with or without CaCl 2 pretreatment mainly resulted from the increase in content of heat stable protein in total soluble protein. It is suggested that Ca 2+ calmodulin may be involved in the synthesis of total soluble protein, and the induction of freezing resistance of seedlings.
文摘The changes in the contents of total soluble protein and RNA, the activity of RNase in leaves and branches of Populus tomentosa cuttings at various periods (viz: cold acclimation, deacclimation, chilling stress and the recovery after chilling stress), and the survival rate and the freezing resistance of cuttings during cold acclimation at -3℃ were investigated. Results showed that cold acclimation not only increased the contents of total soluble protein and RNA, the survival rates and the freezing resistance of cuttings, decreased the activity of RNase, but also reduced the declining degree of total soluble protein and RNA contents, and the increasing level of RNase caused by chilling stress as compared with the controls. In addition, cold acclimation augmented the increase in the level of total soluble protein and RNA, and facilitated the decrease of RNase during the recovery periods. Further analysis found that the DNA content of all treatments kept relative stability at various periods. The changes in total soluble protein, RNA and RNase were closely related to the freezing resistance of cuttings. It appears that the increase of RNA content caused by cold acclimation induced decrease of RNase activity may be involved in the accumulation of total soluble protein and the induction of freezing resistance of cuttings.
基金supported by the National Natural Science Foundation of China (No. 40706050, 40706048 and 30700619)the National Science & Technology Pillar Program (No. 2006BAD01A13 and 2008BAC49B04)+2 种基金National special fund for transgenic project (No. 2009ZX08009-019B)Natural Science Foundation of Shandong Province (No. 2009ZRA02075)and Qingdao Municipal Science and Technology plan project (No. 09-2-5-8-hy)
文摘Total soluble proteins of different life stages, filamentous sporophytes cultivated in high temperatures, and blade gametophytes harvested in different seasons, were identified by SDS-PAGE. The types and amounts of expressed proteins also varied amongst the samples. The fewest soluble proteins were present in filamentous sporophytes. There were more types and amounts of soluble protein in conchospores than in filamentous sporophytes, but fewer than in bulgy sporophytes. More types of protein were detected in filamentous sporophytes cultivated in high temperatures than in those growing in normal situations. The most types and amounts of protein were found in blade gametophytes in all samples. Blade gametophytes harvested last year and stored at -20 ℃ showed only minor differences in expression of proteins when compared with those harvested in different seasons.
文摘Objective To investigate the effects of the total saponin of Dipsacus asperoides (tSDA) and ginsenoside Rb1 (GRb1) on the apoptosis of primary cultured hippocampal neurons induced by β-amyloid protein (Aβ). Methods Primary cultured hippocampal neurons, the cultures were pretreated with tSDA and GRb1 on 10d for 24 hours respectively. Then the cultures were treated with 35 μmol·L -1 Aβ25-35 for 24 hours, observed the changing of survival rate of neurons and the apoptosis of neurons with biochemical analysis combining immunofluorescent cytochemical double-staining technique. Results Hippocampal neurons were treated with 35 μmol·L -1 Aβ for 24 hours, and survival rate of neurons downed to 52.6%. When neurons were pretreated by tSDA and GRb1, survival rate of neurons increased 11% to 15%. The findings of immunofluorescent cytochemical double-staining indicated that apoptotic neurons were obviously more than that of the blank group, reaching 43.9%.When neurons were pretreated by tSDA and GRb1, apoptotic neurons were downed to 16.6%, 10.8% respectively. Conclusion tSDA had the same effects as GRb1, protecting the neurons, antagonizing neurotoxicity of Aβ, increasing survival rate of neurons, and reducing apoptotic neurons induced by Aβ.
基金Supported by the Special Program for Wheat Grain Quality Stability from Ministry of Agriculture of China(070101)the National Key Technologies R&D Program of China during the 10th Five-Year Plan Period(2004BA520A12-4)~~
文摘[Objective] This study aimed to explore the effects of nitrogen fertigation rate on the protein components in grains and processing quality of different wheat varieties. [Method] Under the condition of higher soil fertility, different amounts of N-fertilizer were applied in the plots, and then the contents of total protein and its components, percentage of the component content to total protein content as well as the processing quality of grains of two strong-gluten wheat varieties （Linyou145 and Zhengmai9023） and two weak-gluten wheat varieties （Ningmai9 and Baofeng949） were determined. [Result] The contents of total protein and globulin, gliadin and glutenin were improved significantly with the increase of the N-fertilizer amount; but the content of albumin did not show remarkable increase; in addition, the percentage of each protein component was relative stable and did not increase significantly. Increase in the amount of N-fertilizer improved the sedimentation value, wet gluten content, loaf volume and loaf score, decreased the volume weight of grain. [Conclusion] This study provideed theoretical support for high-quality wheat production.
文摘Background and Aim: Despite the fact that acute appendicitis is the most common surgical emergency all around the world, its diagnosis is still based on clinical evaluation and accuracy of the diagnosis depending on experience. The aim of this study is to evaluate the role of inflammatory markers in diagnosis of acute appendicitis. Material and Method: The study includes 77 cases with histopathologically proven acute appendicitis and 17 control cases. Blood samples were obtained from all cases and C-reactive protein (CRP), Granulocyte Colony Stimulating Factor (G-CSF) and Total Antioxidant Capacity (TAC) were measured. Findings: In cases with acute appendicitis, CRP and G-CSF levels were found to be related to acute appendicitis;however, TAC was not affected by the disease process. Moreover, CRP and G-CSF levels were correlated with the disease severity. Conclusion: Both CRP and G-CSF can be used in diagnosis of acute appendicitis. Furthermore, increased CRP level can be a marker to show advanced cases. However, G-CSF is not an effective marker to show disease severity.
文摘A sensitive and selective assay of proteins is proposed based on measuring the total internal-reflected resonance light scattering(TIR-RLS) signals produced on the water/tetrachloromethane(H_2O/CCl_4) interface. In an aqueous medium with pH value in the range of 3.29—3.78, electrostatic attraction occurs between the negatively charged Evans Blue(EB) and positively charged proteins, forming hydrophobic ion associates and resulting in EB-protein adsorption on H_2O/CCl_4 interface. The presence of cetyltrimethylammonium bromide prompts this adsorption, resulting in strongly enhanced TIR-RLS signals. The intensity of the enhanced TIR-RLS at 360—370 nm was found to be proportional to the concentration of proteins. For bovine serum albumin and human serum albumin, the linear range of detection is 0.07—1.2 μg/mL and the limits of detection are 6.68 and 6.30 ng/mL(3σ), respectively, while for lysozyme, the linear range of detection is 0.06—1.0 μg/mL and the limit of detection is 6.0 ng/mL(3σ). The content of the total albumin in a human urine sample could be directly determined by using the standard addition method with a percent recovery of 97.6%—104.1%, and the RSD ranging from 1.9% to 4.2%.
文摘Posidonia oceanicameadows are experiencing a progressive decline, and monitoring their status is crucial for the maintenance of theseecosystems. We performed a comparativeanalysis of bed density, total phenol content and protein expression pattern to assess the conservation status ofPosidoniaplants from the S. Marinella (Rome, Italy) meadow. The total phenol content was inversely related to maximum beddensity, confirming the relationship betweenhigh phenol content and stressful conditions. In addition, protein expression pattern profilesshowed that the number of differentially expressed proteins was dramatically reduced in the latest years compared to previous analyses. Our results support the usefulness of integrating solid descriptors, such as phenol content, with novel biochemical/molecular approaches in the monitoring of meadows.
文摘The contents of total soluble protein and cowpea trypsin inhibitor (CpTI) in the browse and metaphylla of transgenic hybrid triploid poplars [ (Populus tomentosa×P.bolleana)×P.tomentosa ] transformed with CpTI gene were determined in order to study the products of CpTI gene expression at protein level. The results indicated that the amount of total soluble protein was greater in transgenic poplars than in non transgenic poplars, but was more in the metaphylla than in browse. The expression of CpTI gene resulted in an obvious increase in CpTI content, whereas CpTI was not detected in non transgenic poplars. It was found that there were high amount of total soluble protein and CpTI in 3 clones of TG07, TG04 and TG71 compared with other transgenic clones. In addition, the analysis of protein by SDS PAGE showed that a specific protein band of about 11.3?kD corresponding to the 80 amino acids encoded by the CpTI gene was observed in transgenic poplars on the gel of protein, which was not detected in non transgenic poplars.
文摘为实现南极磷虾(Euphausia superba)品质的快速评定,本研究将近红外光谱技术(Near infrared spectroscopy,NIRS)与偏最小二乘法(Partial least squares,PLS)相结合,构建用于快速预测磷虾体内非蛋白氮(Non-protein nitrogen,NPN)含量和挥发性盐基氮(Total volatile base nitrogen,TVB-N)含量的近红外定量分析模型。采集近红外光谱后,通过比较决定系数(Coefficient of determination,R^(2))、校正标准偏差(Root mean square error of calibration,RMSEC)、预测标准偏差(Root mean square error of prediction,RMSEP)等模型的评价参数,选取近红外光谱定量分析模型的最佳预处理方式、特征光谱范围以及主因子数。结果显示,NPN含量模型的最佳预处理方法为多元散射校正(Multiplicative signal correction,MSC),其特征光谱范围为8887.1~7774.2 cm-1;TVB-N含量模型则采用MSC与卷积平滑(Savitzky-Golay smoothing,SG)相结合的方式进行预处理,建模范围为全波段。两个定量模型的主因子数均为5。经模型的优化与外部验证,最终构建的PLS最优模型如下:NPN含量近红外定量分析模型的R^(2)为0.9384,RMSEC为0.279,RMSEP为0.443;TVB-N含量近红外定量分析模型的R^(2)为0.8685,RMSEC为3.800,RMSEP为4.070。研究结果表明,两个模型均具有良好的预测精度(R^(2)>0.85)和稳定性,其中NPN定量分析模型的预测能力优于TVB-N定量分析模型。综上,本研究基于NIRS与PLS构建的定量分析模型能够有效预测南极磷虾体内的NPN和TVB-N含量,为南极磷虾的品质评价提供了可靠的技术支持,满足快速评定的实际应用需求。
文摘Objectives: To establish an ADL prediction model for Parkinson’s inpatients as an auxiliary evaluation scheme. Methods: The data of Parkinson’s patients hospitalized in the Department of Neurology of Affiliated Brain Hospital of Guangzhou Medical University from 2019 to 2022, which suited the criteria were collected, and a multiple linear regression model was established with serum total protein, serum albumin, age, BMI and education level as independent variables and BI scores as dependent variables. Results: A total of 95 PD patients were included (mean 70.05 ± 10.87 years): 53 males and 42 females. The correlation analysis showed that the serum total protein (r = 0.398, P Conclusion: The ADL multiple linear regression model can be used as an important means to evaluate the ADL ability of PD patients in hospital.
文摘BACKGROUND： The differential diagnosis of many neurodegenerative disorders depends primarily on clinical symptoms together with imaging methods. Recently, increased importance has been placed on the use of biomarkers for diagnosing various neurodegenerative disorders. OBJECTIVE： To assess the feasibility of tau-protein, phosphorylated tau-protein, beta-amyloid 42 （Aβ42）, and 14-3-3 protein as biomarkers for diagnosing several neurodegenerative diseases complicated by cognitive deficits. DESIGN, TIME AND SETTING： A non-randomized, concurrent, case-control investigation was performed in three medical centers in the Czech Republic （Department of Neurology at the University Hospital in Hradec Kralove, Department of Neurology at the 2rd Medical Faculty, and the University Hospital Motol） between October 2000 and November 2006. PARTICIPANTS： Eighteen patients with probable AIzheimer＇s disease, 4 patients with Creutzfeldt-Jakob disease, 10 patients with frontotemporal dementia, 9 patients with clinically isolated syndrome suggestive of multiple sclerosis, and 7 patients with multiple sclerosis, as well as 38 race-, nationality-, and age-matched cognitively intact controls, were included in the study. Diagnoses were established based on the following criteria： the criteria for Alzheimer＇s disease proposed by the National Institute of Neurological and Communicative Disorders and Stroke/Alzheimer＇s Disease and Related Disorders Association, WHO criteria for Creutzfeldt-Jakob disease, Neary criteria for frontotemporal dementia, and McDonald＇s criteria for multiple sclerosis. All included patients were confirmed to suffer from various degrees of dementia. METHODS： Enzyme-linked immunosorbent assay was used to measure concentrations of tau-protein, phosphorylated tau-protein, and Aβ42 in cerebrospinal fluid （CSF） samples collected by standard lumbar puncture from each patient. Moreover, 14-3-3 protein was assessed by Western blot in CSF of Creutzfeldt-Jakob disease patients. Cognitive status was assessed using the Mini Mental Scale Examination （MMSE） in all subjects. MAIN OUTCOME MEASURES： Establishment of biomarkers with greatest specificity and sensitivity for the investigated disorders according to Receiver Operating Characteristic curves, which were based on values from patients and controls; correlation between concentrations of given biomarkers and demographic parameters, diagnosis, duration of disease, and level of cognitive deficit. RESULTS： Increased concentrations of total tau protein and phosphorylated tau protein, and decreased levels of Aβ42, in CSF of Alzheimer＇s disease patients reached the required sensitivity/specificity ratio of 80% or greater. A marked elevation in CSF concentrations of total tau protein showed even greater sensitivity than 14-3-3 protein in Creutzfeldt-Jakob disease. There was no association between selected biomarkers and frontotemporal dementia or multiple sclerosis. Phosphorylated tau-protein was the only biomarker that noticeably correlated with MMSE scores for Alzheimer＇s disease.CONCLUSION： Levels of total tau protein, phosphorylated tau protein, and A!342 in the CSF could differentiate patients with Alzheimer＇s disease and Creutzfeldt-Jakob disease from healthy controls and patients with other neurodegenerative disorders. The diversity of absolute values demonstrates the necessity to establish a specific standard for each laboratory.
