Understanding of the physiological effect of post-full-bloom foliar boron combined with calcium (B+Ca) on apple (<i><span style="font-family:Verdana;">Malus</span></i> <i><sp...Understanding of the physiological effect of post-full-bloom foliar boron combined with calcium (B+Ca) on apple (<i><span style="font-family:Verdana;">Malus</span></i> <i><span style="font-family:Verdana;">domestica</span></i><span style="font-family:Verdana;">) peel tissues is envisaged to give way to the unknown mode-of-action by which these mineral regimens suppress fruit sunburn-browning incidence in orchards. Promotion of this mineral approach among growers, as a certainly cheaper alternative to mitigate fruit sunburn-browning incidence in apple orchards necessitates clear elucidation of its mode-of-action. This study investigated peel photosynthetic pigments and total peroxides (as a measure of oxidative stress) in three apple cultivars, </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> which were treated with four B+Ca treatments varying in levels of B and Ca as well as inclusion of zinc (Zn) in one treatment. Randomized complete block design experiments with five replications were conducted at commercial farms in Western Cape, South Africa. Significant (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < </span><i><span style="font-family:Verdana;">0.05</span></i><span style="font-family:Verdana;">) treatment effect for major pigment aspects and total peroxides occurred in all cultivars, but with strong influence of cultivar and fruit age. For instance, effect of varying B, Ca and possible B+Ca duet-effect on photosynthetic pigments occurred in </span></span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, whereas the Zn-treatment was mainly responsible for significant treatment effects in both </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> apples. Significant treatment effect for total peroxides occurred in </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, yet significant interaction effect occurred with </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, however, these significant results did not yield meaningful peel oxidative stress differences among the treatments. Foliar treatment differences in photochemical efficiency (Fv/Fm) w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> not significant. The study concludes with firm evidence that foliar B+Ca treatment composition has</span><span style="font-family:Verdana;"> a</span><span style="font-family:Verdana;"> significant effect on apple peel photosynthetic pigments depending on cultivar, and Zn is not desirable in the formulation of these treatments.</span>展开更多
Objective:To identify the alleralinn of the membrane polenlial and llie effect of carolenoid extracts from Chlorococcum hnmicola(C.humicola) on membrane hound ATPases and lipid peroxidation.Methods:The lolal carotenoi...Objective:To identify the alleralinn of the membrane polenlial and llie effect of carolenoid extracts from Chlorococcum hnmicola(C.humicola) on membrane hound ATPases and lipid peroxidation.Methods:The lolal carotenoids were extracted from C.humicola.Four groups of Swiss albino mice were treated as control,Benzo(a)pyrene[B(a)P],total carotenoids,B(a)P+ total caralenoids respectively for a period of 60 days.Membrane lipid peroxidation and ATPases(Total ATPases,Ca^(2+)-ATPases.Mg^(2+)-ATPases.Na^+K^+- ATPasei were determined in lung,liver and erythrocyte samples.Results:The activity of lolal ATPase was found to be significantly increased in the B(a)P treated liver and lung tissue.Erythrocyte membrane also showed higher ATPase activity which was significantly reverted on total carolenoid treatment.Conclusions: It can be concluded that the changes in membrane potential favour the functional deterioration of physiological system.The overall findings demonstrates that the animals post treated with carolenoid extract from C.humicola may maintains the alterations in membrane bound ATPase and lipid peroxidation in tissues against the carcinogenic chemical and hence aid in establishing the membrane potential action.Then-fore C.humicola can be further extended to exploits its possible application for various health benefits as neulraceulicals and food additives.展开更多
文摘Understanding of the physiological effect of post-full-bloom foliar boron combined with calcium (B+Ca) on apple (<i><span style="font-family:Verdana;">Malus</span></i> <i><span style="font-family:Verdana;">domestica</span></i><span style="font-family:Verdana;">) peel tissues is envisaged to give way to the unknown mode-of-action by which these mineral regimens suppress fruit sunburn-browning incidence in orchards. Promotion of this mineral approach among growers, as a certainly cheaper alternative to mitigate fruit sunburn-browning incidence in apple orchards necessitates clear elucidation of its mode-of-action. This study investigated peel photosynthetic pigments and total peroxides (as a measure of oxidative stress) in three apple cultivars, </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:;" "=""><span style="font-family:Verdana;"> which were treated with four B+Ca treatments varying in levels of B and Ca as well as inclusion of zinc (Zn) in one treatment. Randomized complete block design experiments with five replications were conducted at commercial farms in Western Cape, South Africa. Significant (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> < </span><i><span style="font-family:Verdana;">0.05</span></i><span style="font-family:Verdana;">) treatment effect for major pigment aspects and total peroxides occurred in all cultivars, but with strong influence of cultivar and fruit age. For instance, effect of varying B, Ca and possible B+Ca duet-effect on photosynthetic pigments occurred in </span></span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, whereas the Zn-treatment was mainly responsible for significant treatment effects in both </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> apples. Significant treatment effect for total peroxides occurred in </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Cripps Pink</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;"> and </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Granny Smith</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, yet significant interaction effect occurred with </span><span style="font-family:Verdana;">‘</span><span style="font-family:Verdana;">Golden Delicious</span><span style="font-family:Verdana;">’</span><span style="font-family:Verdana;">, however, these significant results did not yield meaningful peel oxidative stress differences among the treatments. Foliar treatment differences in photochemical efficiency (Fv/Fm) w</span><span style="font-family:Verdana;">ere</span><span style="font-family:Verdana;"> not significant. The study concludes with firm evidence that foliar B+Ca treatment composition has</span><span style="font-family:Verdana;"> a</span><span style="font-family:Verdana;"> significant effect on apple peel photosynthetic pigments depending on cultivar, and Zn is not desirable in the formulation of these treatments.</span>
基金Supported by Bharathiar university.coimbatore,Tamilnadu India
文摘Objective:To identify the alleralinn of the membrane polenlial and llie effect of carolenoid extracts from Chlorococcum hnmicola(C.humicola) on membrane hound ATPases and lipid peroxidation.Methods:The lolal carotenoids were extracted from C.humicola.Four groups of Swiss albino mice were treated as control,Benzo(a)pyrene[B(a)P],total carotenoids,B(a)P+ total caralenoids respectively for a period of 60 days.Membrane lipid peroxidation and ATPases(Total ATPases,Ca^(2+)-ATPases.Mg^(2+)-ATPases.Na^+K^+- ATPasei were determined in lung,liver and erythrocyte samples.Results:The activity of lolal ATPase was found to be significantly increased in the B(a)P treated liver and lung tissue.Erythrocyte membrane also showed higher ATPase activity which was significantly reverted on total carolenoid treatment.Conclusions: It can be concluded that the changes in membrane potential favour the functional deterioration of physiological system.The overall findings demonstrates that the animals post treated with carolenoid extract from C.humicola may maintains the alterations in membrane bound ATPase and lipid peroxidation in tissues against the carcinogenic chemical and hence aid in establishing the membrane potential action.Then-fore C.humicola can be further extended to exploits its possible application for various health benefits as neulraceulicals and food additives.
