Fluorescence liftime imaging （FLIM） of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their envi...Fluorescence liftime imaging （FLIM） of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incubating cells with these dyes, we find a punctate and continuous distribution of the dye in cells. The viscosity value obtained in what appears to be endocytotic vesicles in living cells is around 100 times higher than that of water and of cellular cytoplasm.Time-resolved fluorescence anisotropy measurements also yield rotational correlation times consistent with large microviscosity values. In this way, we successfully develop a practical and versatile approach to map the microviscosity in cells based on imaging fluorescent molecular rotors.展开更多
移动机器人即时定位与建图(SLAM)技术研究越来越多,但移动机器人SLAM技术存在一些问题,如在构建地图中进行回环匹配时,由于数据信息相似或者数据采集模糊易造成回环出错,从而降低地图精度。对CartographerSLAM在回环检测时使用ScantoMa...移动机器人即时定位与建图(SLAM)技术研究越来越多,但移动机器人SLAM技术存在一些问题,如在构建地图中进行回环匹配时,由于数据信息相似或者数据采集模糊易造成回环出错,从而降低地图精度。对CartographerSLAM在回环检测时使用ScantoMap匹配出现特征点缺失问题进行改进,提出Map to Map策略。该方法利用子图间的匹配闭环检测,去掉连续帧之间的冗余数据,减少运算时间,提高精度。对改进算法结果进行实验对比,选取相关点计算误差作出折线图,可直观看出改进后的算法建图误差比改进前的误差小。展开更多
文摘Fluorescence liftime imaging （FLIM） of modified hydrophobic bodipy dyes that act as fluorescent molecular rotors shows that the fluorescence lifetime of these probes is a function of the microviscosity of their environment. Incubating cells with these dyes, we find a punctate and continuous distribution of the dye in cells. The viscosity value obtained in what appears to be endocytotic vesicles in living cells is around 100 times higher than that of water and of cellular cytoplasm.Time-resolved fluorescence anisotropy measurements also yield rotational correlation times consistent with large microviscosity values. In this way, we successfully develop a practical and versatile approach to map the microviscosity in cells based on imaging fluorescent molecular rotors.
文摘移动机器人即时定位与建图(SLAM)技术研究越来越多,但移动机器人SLAM技术存在一些问题,如在构建地图中进行回环匹配时,由于数据信息相似或者数据采集模糊易造成回环出错,从而降低地图精度。对CartographerSLAM在回环检测时使用ScantoMap匹配出现特征点缺失问题进行改进,提出Map to Map策略。该方法利用子图间的匹配闭环检测,去掉连续帧之间的冗余数据,减少运算时间,提高精度。对改进算法结果进行实验对比,选取相关点计算误差作出折线图,可直观看出改进后的算法建图误差比改进前的误差小。
