A new complex Tb(IB)_3(phen)·2H_2O that gives off green fluorescence was synthesized. The results show that the strong fluorescence emitting of 490 and 545 nm for Tb 3+ in the Tb(IB)_3(phen)·2H_2O complex is...A new complex Tb(IB)_3(phen)·2H_2O that gives off green fluorescence was synthesized. The results show that the strong fluorescence emitting of 490 and 545 nm for Tb 3+ in the Tb(IB)_3(phen)·2H_2O complex is related to the transitions 5D_4-7F_6 and 5D_4-7F_5, respectively. The results indicate that the complex is concentrated on the nuclei regions in the section of onion toot tip tissues and the nuclei are high lighted under fluorescent microscope. It is possible that the Tb(IB)_3(phen)·2H_2O complex can be developed as a fluorescent dye in biological and pathological studies.展开更多
Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and e...Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and eosin(H&E)stained tissues with high space-bandwidth and reduced artifacts.However,current FPM implementations require either precise system calibration and high-quality raw data,or significant computational loads due to iterative algorithms,which limits the practicality of FPM in routine pathological examinations.In this work,latent wavefront denoting the unobservable exiting wave at the surface of the sensor is introduced.A latent wavefront physical model optimized with variational expectation maximization(VEM)is proposed to tackle the inverse problem of FPM.The VEM-FPM alternates between solving a non-convex optimization problem as the main task for the latent wavefront in the spatial domain and merging together their Fourier spectrum in the Fourier plane as an intermediate product by solving a convex closed-formed Fourier space optimization.The VEM-FPM approach enables a stitching-free,full-field reconstruction for Fourier ptychography over a 5.3 mm×5.3 mm field of view,using a 2.5×objective with a numerical aperture(NA)of 0.08.The synthetic aperture achieves a resolution equivalent to 0.53 NA at 532 nm wavelength.The execution speed of VEM-FPM is twice as fast as that of state-of-the-art feature-domain methods while maintaining comparable reconstruction quality.展开更多
文摘A new complex Tb(IB)_3(phen)·2H_2O that gives off green fluorescence was synthesized. The results show that the strong fluorescence emitting of 490 and 545 nm for Tb 3+ in the Tb(IB)_3(phen)·2H_2O complex is related to the transitions 5D_4-7F_6 and 5D_4-7F_5, respectively. The results indicate that the complex is concentrated on the nuclei regions in the section of onion toot tip tissues and the nuclei are high lighted under fluorescent microscope. It is possible that the Tb(IB)_3(phen)·2H_2O complex can be developed as a fluorescent dye in biological and pathological studies.
基金National Natural Science Foundation of China(62235009)。
文摘Fourier ptychographic microscopy(FPM)is a promising technique for achieving high-resolution and large fieldof-view imaging,which is particularly suitable for pathological applications,such as imaging hematoxylin and eosin(H&E)stained tissues with high space-bandwidth and reduced artifacts.However,current FPM implementations require either precise system calibration and high-quality raw data,or significant computational loads due to iterative algorithms,which limits the practicality of FPM in routine pathological examinations.In this work,latent wavefront denoting the unobservable exiting wave at the surface of the sensor is introduced.A latent wavefront physical model optimized with variational expectation maximization(VEM)is proposed to tackle the inverse problem of FPM.The VEM-FPM alternates between solving a non-convex optimization problem as the main task for the latent wavefront in the spatial domain and merging together their Fourier spectrum in the Fourier plane as an intermediate product by solving a convex closed-formed Fourier space optimization.The VEM-FPM approach enables a stitching-free,full-field reconstruction for Fourier ptychography over a 5.3 mm×5.3 mm field of view,using a 2.5×objective with a numerical aperture(NA)of 0.08.The synthetic aperture achieves a resolution equivalent to 0.53 NA at 532 nm wavelength.The execution speed of VEM-FPM is twice as fast as that of state-of-the-art feature-domain methods while maintaining comparable reconstruction quality.
