Dental implants are the primary solution for tooth replacement,providing both aesthetic and functional restoration.Their long-term success depends not only on osseointegration but also on robust peri-implant soft tiss...Dental implants are the primary solution for tooth replacement,providing both aesthetic and functional restoration.Their long-term success depends not only on osseointegration but also on robust peri-implant soft tissue integration(PSTI),particularly in the transmucosal region,where a stable epithelial seal is critical to preventing microbial infiltration and peri-implant inflammation.While surface topography modifications such as roughness,morphology,and porosity influence gingival cell behavior,passive surface modifications alone are often insufficient to promote rapid PSTI.This raises a fundamental question in dental implant design:How can implant surfaces be bioengineered to actively promote PSTI rather than passively relying on cellular responses?This review examines how biofunctionalization has emerged as a transformative strategy in implant surface engineering and critically analyses the latest biofunctionalization strategies for dental implants,with a particular focus on the underlying mechanisms that regulate biomolecule-implant interactions.It evaluates biomolecule incorporation via physical and covalent attachment,highlighting their distinct advantages in stability,efficiency,and scalability.We discuss approaches for functionalizing dental implant surfaces with bioactive molecules,such as proteins and peptides,and cells to replicate natural biological interactions,regulate immune responses,and enhance antimicrobial defense mechanisms.By addressing how bioengineered surfaces can be designed to actively engage with biological systems,this review provides a framework for developing next-generation implant technologies that achieve more effective and predictable PSTI,with strong potential for clinical translation.展开更多
Soft tissue integration around titanium(Ti)implants is weaker than that around natural teeth,compromising long-term success of Ti implants.Carbon monoxide(CO)possesses distinctive therapeutic properties,rendering it a...Soft tissue integration around titanium(Ti)implants is weaker than that around natural teeth,compromising long-term success of Ti implants.Carbon monoxide(CO)possesses distinctive therapeutic properties,rendering it as a highly promising candidate for enhancing STI.However,achieving controlled CO generation at the STI interface remains challenging.Herein,a controlled CO-releasing dual-function coating was constructed on Ti surfaces.Under near-infrared(NIR)irradiation,the designed surface could actively accelerate CO generation for antibiosis against both aerobic and anaerobic bacteria.More importantly,in the absence of NIR,the slow release of CO induces macrophage polarization from pro-inflammatory phenotype towards pro-regenerative phenotype.In a rat implantation model with induced infection,the designed surface effectively controlled the bacterial infection,alleviates accompanying inflammation and modulated immune microenvironment,leading to enhanced STI.Single-cell sequencing revealed that the coating alters the cytokine profile within the soft tissue,thereby influencing cellular functions.Differentially expressed genes in macrophages are highly enriched in the PIK3-Akt pathway.Furthermore,the cellular communication between fibroblasts and macrophages was significantly enhanced through the CXCL12/CXCL14/CXCR4 and CSF1-CSF1R ligand-receptor pair.These findings indicate that our coating showed an appealing prospect for enhancing STI around Ti implants,which would ultimately contribute to the improved long-term success of Ti implants.展开更多
Biomaterials are increasingly being evolved to actively adapt to the desired microenvironments so as to introduce tissue integration, reconstruct stability, promote regeneration, and avoid immune rejection. The comple...Biomaterials are increasingly being evolved to actively adapt to the desired microenvironments so as to introduce tissue integration, reconstruct stability, promote regeneration, and avoid immune rejection. The complexity of its mechanisms poses great challenge to current biomimetic synthetic materials. Although still at initial stage, harnessing cells, tissues, or even entire body to synthesize bioadaptive materials is introducing a promising future.展开更多
Spatial transcriptomics is undergoing rapid advancements and iterations.It is a beneficial tool to significantly enhance our understanding of tissue organization and relationships between cells.Recent technological ad...Spatial transcriptomics is undergoing rapid advancements and iterations.It is a beneficial tool to significantly enhance our understanding of tissue organization and relationships between cells.Recent technological advancements have achieved subcellular resolution,providing much denser spot placement for downstream analysis.A key challenge for this following analysis is accurate cell segmentation and the assignment of spots to individual cells.The primary objective of this study was to evaluate the effectiveness of a new cell segmentation approach based on subcellular level spatial transcriptomic data by confirming nuclei positions and using Voronoi diagrams,compared to direct clustering with cellbin data.Our findings demonstrate that the Voronoi method not only outperforms traditional methods in providing clearer boundaries and better separation of cell types,but also excels in preserving the most transcripts,addressing the issue of low capture efficiency.This integrative methodology presents a substantial advancement in spatial transcriptomics,offering improved cell type classification and spatial pattern recognition.展开更多
基金the support of the International Team for Implantology(ITI,Grant No:1796-2023)the Australian Research Council through the Discovery Early Career Researcher Award(DECRA,DE210100662).
文摘Dental implants are the primary solution for tooth replacement,providing both aesthetic and functional restoration.Their long-term success depends not only on osseointegration but also on robust peri-implant soft tissue integration(PSTI),particularly in the transmucosal region,where a stable epithelial seal is critical to preventing microbial infiltration and peri-implant inflammation.While surface topography modifications such as roughness,morphology,and porosity influence gingival cell behavior,passive surface modifications alone are often insufficient to promote rapid PSTI.This raises a fundamental question in dental implant design:How can implant surfaces be bioengineered to actively promote PSTI rather than passively relying on cellular responses?This review examines how biofunctionalization has emerged as a transformative strategy in implant surface engineering and critically analyses the latest biofunctionalization strategies for dental implants,with a particular focus on the underlying mechanisms that regulate biomolecule-implant interactions.It evaluates biomolecule incorporation via physical and covalent attachment,highlighting their distinct advantages in stability,efficiency,and scalability.We discuss approaches for functionalizing dental implant surfaces with bioactive molecules,such as proteins and peptides,and cells to replicate natural biological interactions,regulate immune responses,and enhance antimicrobial defense mechanisms.By addressing how bioengineered surfaces can be designed to actively engage with biological systems,this review provides a framework for developing next-generation implant technologies that achieve more effective and predictable PSTI,with strong potential for clinical translation.
基金support from the Natural Science Foundation of China(52073224 and 52073230)the Shaanxi Provincial Science Fund for Distinguished Young Scholars(2023-JC-JQ-32)+1 种基金Key Research and Development Program of Shaanxi(2024SFYBXM-438 and 2022SF-165)Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0225).
文摘Soft tissue integration around titanium(Ti)implants is weaker than that around natural teeth,compromising long-term success of Ti implants.Carbon monoxide(CO)possesses distinctive therapeutic properties,rendering it as a highly promising candidate for enhancing STI.However,achieving controlled CO generation at the STI interface remains challenging.Herein,a controlled CO-releasing dual-function coating was constructed on Ti surfaces.Under near-infrared(NIR)irradiation,the designed surface could actively accelerate CO generation for antibiosis against both aerobic and anaerobic bacteria.More importantly,in the absence of NIR,the slow release of CO induces macrophage polarization from pro-inflammatory phenotype towards pro-regenerative phenotype.In a rat implantation model with induced infection,the designed surface effectively controlled the bacterial infection,alleviates accompanying inflammation and modulated immune microenvironment,leading to enhanced STI.Single-cell sequencing revealed that the coating alters the cytokine profile within the soft tissue,thereby influencing cellular functions.Differentially expressed genes in macrophages are highly enriched in the PIK3-Akt pathway.Furthermore,the cellular communication between fibroblasts and macrophages was significantly enhanced through the CXCL12/CXCL14/CXCR4 and CSF1-CSF1R ligand-receptor pair.These findings indicate that our coating showed an appealing prospect for enhancing STI around Ti implants,which would ultimately contribute to the improved long-term success of Ti implants.
基金supported by the National Basic Research Program of China(973 Program,No.2012CB619105)the China Postdoctoral Science Foundation(Nos.2013M531876 and 2014T70826)+4 种基金the National Natural Science Foundation of China(Nos.31430030,81272041,81071512 and 31170902)the Natural Science Foundation of Guangdong Province(Nos.2014A030310466 and 2013B060300007)the Foundation of Shenzhen Committee for Science and Technology Innovation(Nos.CXZZ20130516103023168 and 2015-336,2013-950)the Jiangxi Province Science and Technology Support Plan Project(No.2010BSA14800)the Guangdong Provincial Key Laboratory of Orthopaedics and Tranmstology
文摘Biomaterials are increasingly being evolved to actively adapt to the desired microenvironments so as to introduce tissue integration, reconstruct stability, promote regeneration, and avoid immune rejection. The complexity of its mechanisms poses great challenge to current biomimetic synthetic materials. Although still at initial stage, harnessing cells, tissues, or even entire body to synthesize bioadaptive materials is introducing a promising future.
文摘Spatial transcriptomics is undergoing rapid advancements and iterations.It is a beneficial tool to significantly enhance our understanding of tissue organization and relationships between cells.Recent technological advancements have achieved subcellular resolution,providing much denser spot placement for downstream analysis.A key challenge for this following analysis is accurate cell segmentation and the assignment of spots to individual cells.The primary objective of this study was to evaluate the effectiveness of a new cell segmentation approach based on subcellular level spatial transcriptomic data by confirming nuclei positions and using Voronoi diagrams,compared to direct clustering with cellbin data.Our findings demonstrate that the Voronoi method not only outperforms traditional methods in providing clearer boundaries and better separation of cell types,but also excels in preserving the most transcripts,addressing the issue of low capture efficiency.This integrative methodology presents a substantial advancement in spatial transcriptomics,offering improved cell type classification and spatial pattern recognition.
