Many clinical studies have addressed the treatment of acute cerebral hemorrhage using acupuncture. However, few studies have examined the relationship between time of acupuncture and curative effect on cerebral hemorr...Many clinical studies have addressed the treatment of acute cerebral hemorrhage using acupuncture. However, few studies have examined the relationship between time of acupuncture and curative effect on cerebral hemorrhage. By observing the effect of acupuncture on changes in histopathology, ultrastructure, and neuroethology in a cerebral hemorrhage model of rats, we have directly examined the time-effect relationship of acupuncture. The rat model of cerebral hemorrhage was produced by slowly injecting autologous blood to the right caudate nucleus. The experimental groups were: 3-, 9-, 24-, and 48-hour model groups; and 3-, 9-, 24-, and 48-hour acupuncture groups. The sham-operation group was used for comparison. Acupuncture was performed at the Neiguan(PC6) and Renzhong(DU26) acupoints, twice a day, 6 hours apart, for 5 consecutive days. Brain tissue changes were observed by light microscopy and transmission electron microscopy. Neuroethology was assessed using Bederson and Longa scores. Our results show that compared with the sham-operation and model groups, Bederson and Longa scores were lower in each acupuncture group, with visibly improved histopathology and brain tissue ultrastructure. Further, the results were better in the 3-and 9-hour acupuncture groups than the 24-and 48-hour acupuncture groups. Our findings show that acupuncture treatment can relieve pathological and ultrastructural deterioration and neurological impairment caused by the acute phase of cerebral hemorrhage, and may protect brain tissue during this period. In addition, earlier acupuncture intervention following cerebral hemorrhage(by 3 or 9 hours) is associated with a better treatment outcome.展开更多
Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided ...Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided into 19 groups （n- 10）： the vehicle control group, the PHAAC groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h） and the prasugrel hydrochloride groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h）. Rats were singly intra- gastic administration of the vehicle, the PHAAC （5 mg·kg^-1） or the prasugrel hydrochloride （5 mg · kg^-1 ）, re- spectively. Blood samples were taken at each time point for the determination of platelet aggregation rate （PAR）. For the dose-effect study, 110 SD rats were devided into 11 groups （n= 10）： the vehicle control group, the PHAAC groups （10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） and the prasugrel hydrochloride groups （ 10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） . Blood samples were taken at 4 h after drug administration for the determination of PAR. Results Compared with the vehicle group, PHAAC has significant anti-platelet ag- gregative effects （P 〈 0.05） at the time of 0.5, 1, 2, 4, 6, 24, 48 h, and the effect at the time of 4 h was the strongest. There were no obvious differences between the effect of PHAAC （5 mg · kg^-1） and prasugrel hydrochlo- ride （5 mg · kg^-1） at each time point. Compared with the vehicle group, intragastic administration of PHAAC at the doses of 10, 5, 2.5, 1, 0.5 mg · kg^-1 could obviously inhibite the platelet aggregation, and showed a dose- dependent manner. There were no significant differences between the effect of PHAAC and prasugrel hydrochloride at the same dose. Conclusion PHAAC can inhibit platelet aggregation in a dose-dependent manner, and the effect at 4 h after drug administration is the strongest. The action strength and duration of PHAAC are similar with that of the prasugrel hydrochloride.展开更多
Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmeno...Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmenorrhea were randomly allocated into a group of single point. In each treatment, the needles retained for 30 min and 15 person times were observed in each group. The VAS （Visual Analogue Scale） values were recorded immediately before needling, 5 min, 20 min and 30 min upon insertion, and 30 min, 60 min and 120 min upon withdrawal for statistical analysis. Results： Immediate pain relief occurred following acupuncture and the pain-alleviating effect was incremented within the 30 min of needle retaining. At 20 min upon needle insertion, the pain relief from needling multiple points appeared substantially stronger than needling a single point （P〈0.05） and this effect remained until 2 h after withdraw of the needles. Conclusion： Needling a single point or multiple points can both produce remarkably immediate pain relief for patients with severe primary dysmenorrhea and share similar time-effect relationship. However, needling multiple points showed substantially better effects than needling a single point in a number of time points.展开更多
目的:评估不同时段运动对血糖水平、脂质代谢、疲劳感知及心血管健康等健康指标的影响差异,并探讨其科学基础与实践应用价值。方法:系统检索PubMed、Web of Science、Embase、Scopus及中国知网(CNKI)等中外文数据库,整合相关文献回顾结...目的:评估不同时段运动对血糖水平、脂质代谢、疲劳感知及心血管健康等健康指标的影响差异,并探讨其科学基础与实践应用价值。方法:系统检索PubMed、Web of Science、Embase、Scopus及中国知网(CNKI)等中外文数据库,整合相关文献回顾结果与实验数据进行综合分析。结果:与早晨(8:00—10:00)运动相比,晚间(18:00—20:00)运动在调节血糖稳态和改善脂质代谢方面具有显著优势,表现为更优的糖耐量与胰岛素敏感性;下午(15:00—18:00)运动虽受益于核心体温升高0.8℃带来的生理优势及较低的疲劳感知,但伴随肌肉损伤标志物(CK、LDH等)水平升高,需延长恢复时间。此外,运动时间对血压调节具有显著时相效应,晚间运动诱导的后运动低血压(PEH)效应更显著,振幅达7.2 mmHg,对高血压患者尤为有益。结论:基于昼夜节律优化运动时间可最大化健康效益,下午至晚间(15:00—20:00)为综合效益最优时段。其中,降血糖相关训练建议安排在15:00—18:00,降压相关训练推荐于18:00—20:00开展。个体化运动方案的制定需充分考虑时型(chronotype)分型特征,晚型人群应避免晨间高强度运动,以降低心血管事件风险。建议:未来研究可聚焦三方面内容,一是饮食与运动的交互作用机制,二是不同运动模式的时相效应差异,三是长期择时运动在慢性病管理中的分子调控机制。展开更多
基金supported by a grant from the Tianjin Science and Technology Commission,China,No.05YFSZSF02600(to PL)
文摘Many clinical studies have addressed the treatment of acute cerebral hemorrhage using acupuncture. However, few studies have examined the relationship between time of acupuncture and curative effect on cerebral hemorrhage. By observing the effect of acupuncture on changes in histopathology, ultrastructure, and neuroethology in a cerebral hemorrhage model of rats, we have directly examined the time-effect relationship of acupuncture. The rat model of cerebral hemorrhage was produced by slowly injecting autologous blood to the right caudate nucleus. The experimental groups were: 3-, 9-, 24-, and 48-hour model groups; and 3-, 9-, 24-, and 48-hour acupuncture groups. The sham-operation group was used for comparison. Acupuncture was performed at the Neiguan(PC6) and Renzhong(DU26) acupoints, twice a day, 6 hours apart, for 5 consecutive days. Brain tissue changes were observed by light microscopy and transmission electron microscopy. Neuroethology was assessed using Bederson and Longa scores. Our results show that compared with the sham-operation and model groups, Bederson and Longa scores were lower in each acupuncture group, with visibly improved histopathology and brain tissue ultrastructure. Further, the results were better in the 3-and 9-hour acupuncture groups than the 24-and 48-hour acupuncture groups. Our findings show that acupuncture treatment can relieve pathological and ultrastructural deterioration and neurological impairment caused by the acute phase of cerebral hemorrhage, and may protect brain tissue during this period. In addition, earlier acupuncture intervention following cerebral hemorrhage(by 3 or 9 hours) is associated with a better treatment outcome.
文摘Aim To evaluate the time-effect and dose-effect of prasugrel hydrobromide acetic acid compound （PHAAC） inhibiting platelet aggregation. Methods For the time-effect study, 190 Sprague-Dawley （SD） rats were devided into 19 groups （n- 10）： the vehicle control group, the PHAAC groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h） and the prasugrel hydrochloride groups （0.5, 1, 2, 4, 6, 24, 48, 72, 96 h）. Rats were singly intra- gastic administration of the vehicle, the PHAAC （5 mg·kg^-1） or the prasugrel hydrochloride （5 mg · kg^-1 ）, re- spectively. Blood samples were taken at each time point for the determination of platelet aggregation rate （PAR）. For the dose-effect study, 110 SD rats were devided into 11 groups （n= 10）： the vehicle control group, the PHAAC groups （10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） and the prasugrel hydrochloride groups （ 10, 5, 2.5, 1, 0.5 mg · kg^-1, dosage of prasugrel） . Blood samples were taken at 4 h after drug administration for the determination of PAR. Results Compared with the vehicle group, PHAAC has significant anti-platelet ag- gregative effects （P 〈 0.05） at the time of 0.5, 1, 2, 4, 6, 24, 48 h, and the effect at the time of 4 h was the strongest. There were no obvious differences between the effect of PHAAC （5 mg · kg^-1） and prasugrel hydrochlo- ride （5 mg · kg^-1） at each time point. Compared with the vehicle group, intragastic administration of PHAAC at the doses of 10, 5, 2.5, 1, 0.5 mg · kg^-1 could obviously inhibite the platelet aggregation, and showed a dose- dependent manner. There were no significant differences between the effect of PHAAC and prasugrel hydrochloride at the same dose. Conclusion PHAAC can inhibit platelet aggregation in a dose-dependent manner, and the effect at 4 h after drug administration is the strongest. The action strength and duration of PHAAC are similar with that of the prasugrel hydrochloride.
基金Key Project of the National Eleventh-Five Year Research Program of China (2006BAI12B06)Shandong Science & Technology Development Program of Traditional Chinese Medicine (2009-135)
文摘Objective： To observe the time-effect relationship of needling a single point or multiple points for pain relief in patients with severe primary dysmenorrhea. Methods： A total of 20 cases with severe primary dysmenorrhea were randomly allocated into a group of single point. In each treatment, the needles retained for 30 min and 15 person times were observed in each group. The VAS （Visual Analogue Scale） values were recorded immediately before needling, 5 min, 20 min and 30 min upon insertion, and 30 min, 60 min and 120 min upon withdrawal for statistical analysis. Results： Immediate pain relief occurred following acupuncture and the pain-alleviating effect was incremented within the 30 min of needle retaining. At 20 min upon needle insertion, the pain relief from needling multiple points appeared substantially stronger than needling a single point （P〈0.05） and this effect remained until 2 h after withdraw of the needles. Conclusion： Needling a single point or multiple points can both produce remarkably immediate pain relief for patients with severe primary dysmenorrhea and share similar time-effect relationship. However, needling multiple points showed substantially better effects than needling a single point in a number of time points.
文摘目的:评估不同时段运动对血糖水平、脂质代谢、疲劳感知及心血管健康等健康指标的影响差异,并探讨其科学基础与实践应用价值。方法:系统检索PubMed、Web of Science、Embase、Scopus及中国知网(CNKI)等中外文数据库,整合相关文献回顾结果与实验数据进行综合分析。结果:与早晨(8:00—10:00)运动相比,晚间(18:00—20:00)运动在调节血糖稳态和改善脂质代谢方面具有显著优势,表现为更优的糖耐量与胰岛素敏感性;下午(15:00—18:00)运动虽受益于核心体温升高0.8℃带来的生理优势及较低的疲劳感知,但伴随肌肉损伤标志物(CK、LDH等)水平升高,需延长恢复时间。此外,运动时间对血压调节具有显著时相效应,晚间运动诱导的后运动低血压(PEH)效应更显著,振幅达7.2 mmHg,对高血压患者尤为有益。结论:基于昼夜节律优化运动时间可最大化健康效益,下午至晚间(15:00—20:00)为综合效益最优时段。其中,降血糖相关训练建议安排在15:00—18:00,降压相关训练推荐于18:00—20:00开展。个体化运动方案的制定需充分考虑时型(chronotype)分型特征,晚型人群应避免晨间高强度运动,以降低心血管事件风险。建议:未来研究可聚焦三方面内容,一是饮食与运动的交互作用机制,二是不同运动模式的时相效应差异,三是长期择时运动在慢性病管理中的分子调控机制。
