Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such a...Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such as biomedicine,green energy,and environmental monitoring.However,due to limitations in imaging framerate and realtime data processing,the real-time throughput of existing IFC systems has been restricted to approximately 1000-10,000 events per second(eps),which is insufficient for large-scale cell analysis.In this work,we demonstrate IFC with real-time throughput exceeding 1,000,000 eps by integrating optical time-stretch(OTS)imaging,microfluidic-based cell manipulation,and online image processing.Cells flowing at speeds up to 15 m/s are clearly imaged with a spatial resolution of 780 nm,and images of each individual cell are captured,stored,and analyzed.The capabilities and performance of our system are validated through the identification of malignancies in clinical colorectal samples.This work sets a new record for throughput in imaging flow cytometry,and we believe it has the potential to revolutionize cell analysis by enabling highly efficient,accurate,and intelligent measurement.展开更多
A tightly synchronized fiber laser system composed of two mode-locked Yb-doped fiber lasers in a master-slave configuration is built.The synchronization could sustain for more than 6 h,and the maximum tolerance of cav...A tightly synchronized fiber laser system composed of two mode-locked Yb-doped fiber lasers in a master-slave configuration is built.The synchronization could sustain for more than 6 h,and the maximum tolerance of cavity length mismatch is measured to be about 210μm.Afterward,a time-stretch dispersive Fourier transform technique is introduced to analyze the synchronization process over multiple cycles.The pulse evolution,center wavelength shift,spectral reshaping,and broadening are all clearly detected.And the synchronization time is experimentally determined on the order of microseconds(hundreds of roundtrips).These results also show the seed pulse acting as a temporal gate for mode locking in some cases.To the best of our knowledge,this is the first time that pulse formation,spectral evolution,center wavelength shift,and synchronization time during the synchronization process are precisely revealed in experiment.These results would help to improve the performances of synchronized laser devices and deeply understand the mechanisms of the synchronization process and other light-light interactions in materials.展开更多
Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, ...Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, such as magnetic resonance imaging （MRI）, aerospace remote sensing, terahertz photography, and hyperspectral imaging. Compared with conventional silicon-based cameras, single-pixel cameras （SPCs） can achieve image compression and operate over a much broader spectral range. However, the imaging speed of SPCs is governed by the response time of digital mieromirror devices （DMDs） and the amount of com- pression of acquired images, leading to low （ms-level） temporal resolution. Consequently, it is particularly challenging for SPCs to investigate fast dynamic phenomena, which is required commonly in microscopy. Recently, a unique approach based on photonic time stretch （PTS） to achieve high-speed SPI has been reported. It achieves a frame rate far beyond that can be reached with conventional SPCs. In this paper, we first introduce the principles and applications of the PTS technique. Then the basic archi- tecture of the high-speed SPI system is presented, and an imaging flow cytometer with high speed and high throughput is demonstrated experimentally. Finally, the limitations and potential applications of high-speed SPI are discussed.展开更多
Raman scattering spectroscopy is widely used as an analytical technique in various fields,but its measurement process tends to be slow due to the low scattering cross-section.In the last decade,various broadband coher...Raman scattering spectroscopy is widely used as an analytical technique in various fields,but its measurement process tends to be slow due to the low scattering cross-section.In the last decade,various broadband coherent Raman scattering spectroscopy techniques have been developed to address this limitation,achieving a measurement rate of 500 kSpectra/s.Here,we present a substantially increased measurement rate of 50 MSpectra/s,which is 100 times higher than the previous state-of-the-art,by developing time-stretch coherent Raman scattering spectroscopy.Our newly developed system,based on a mode-locked Yb fiber laser,enables highly efficient broadband excitation of molecular vibrations via impulsive stimulated Raman scattering with an ultrashort femtosecond pulse and sensitive time-stretch detection with a picosecond probe pulse at a high repetition rate of the laser.As a proof-of-concept demonstration,we measure broadband coherent Stokes Raman scattering spectra of organic compounds covering the molecular fingerprint region from 200 to 1,200 cm^(-1).This high-speed broadband vibrational spectroscopy technique holds promise for unprecedented measurements of sub-microsecond dynamics of irreversible phenomena and extremely high-throughput measurements.展开更多
基金supported by the National Key R&D Program of China(2023YFF0723300)National Natural Science Foundation of China(62475198,62075200,12374295)+8 种基金Fundamental Research Funds for the Central Universities(2042024kf0003,2042024kf1010,2042023kf0105)Hubei Provincial Natural Science Foundation of China(2023AFB133)Jiangsu Science and Technology Program(BK20221257)Shenzhen Science and Technology Program(JCYJ20220530140601003,JCYJ20230807090207014)Translational Medicine and Multidisciplinary Research Project of Zhongnan Hospital of Wuhan University(ZNJC202217,ZNJC202232)The Interdisciplinary Innovative Talents Foundation from Renmin Hospital of Wuhan University(JCRCYR-2022-006)Hubei Province Young Science and Technology Talent Morning Hight Lift Project(202319)The Fund of National Key Laboratory of Plasma Physics(6142A04230201)We gratefully acknowledge Serendipity Lab for facilitating collaboration opportunities.
文摘Imaging flow cytometry(IFC)combines the imaging capabilities of microscopy with the high throughput of flow cytometry,offering a promising solution for high-precision and high-throughput cell analysis in fields such as biomedicine,green energy,and environmental monitoring.However,due to limitations in imaging framerate and realtime data processing,the real-time throughput of existing IFC systems has been restricted to approximately 1000-10,000 events per second(eps),which is insufficient for large-scale cell analysis.In this work,we demonstrate IFC with real-time throughput exceeding 1,000,000 eps by integrating optical time-stretch(OTS)imaging,microfluidic-based cell manipulation,and online image processing.Cells flowing at speeds up to 15 m/s are clearly imaged with a spatial resolution of 780 nm,and images of each individual cell are captured,stored,and analyzed.The capabilities and performance of our system are validated through the identification of malignancies in clinical colorectal samples.This work sets a new record for throughput in imaging flow cytometry,and we believe it has the potential to revolutionize cell analysis by enabling highly efficient,accurate,and intelligent measurement.
基金Natural Science Foundation of Beijing Municipality(4192015)National Natural Science Foundation of China(61975003)。
文摘A tightly synchronized fiber laser system composed of two mode-locked Yb-doped fiber lasers in a master-slave configuration is built.The synchronization could sustain for more than 6 h,and the maximum tolerance of cavity length mismatch is measured to be about 210μm.Afterward,a time-stretch dispersive Fourier transform technique is introduced to analyze the synchronization process over multiple cycles.The pulse evolution,center wavelength shift,spectral reshaping,and broadening are all clearly detected.And the synchronization time is experimentally determined on the order of microseconds(hundreds of roundtrips).These results also show the seed pulse acting as a temporal gate for mode locking in some cases.To the best of our knowledge,this is the first time that pulse formation,spectral evolution,center wavelength shift,and synchronization time during the synchronization process are precisely revealed in experiment.These results would help to improve the performances of synchronized laser devices and deeply understand the mechanisms of the synchronization process and other light-light interactions in materials.
基金Project supported by the National Natural Science Foundation of China （Nos. 61771284 and 61322113）
文摘Single-pixel imaging （SPI） technology has garnered great interest within the last decade because of its ability to record high-resolution images using a single-pixel detector. It has been applied to diverse fields, such as magnetic resonance imaging （MRI）, aerospace remote sensing, terahertz photography, and hyperspectral imaging. Compared with conventional silicon-based cameras, single-pixel cameras （SPCs） can achieve image compression and operate over a much broader spectral range. However, the imaging speed of SPCs is governed by the response time of digital mieromirror devices （DMDs） and the amount of com- pression of acquired images, leading to low （ms-level） temporal resolution. Consequently, it is particularly challenging for SPCs to investigate fast dynamic phenomena, which is required commonly in microscopy. Recently, a unique approach based on photonic time stretch （PTS） to achieve high-speed SPI has been reported. It achieves a frame rate far beyond that can be reached with conventional SPCs. In this paper, we first introduce the principles and applications of the PTS technique. Then the basic archi- tecture of the high-speed SPI system is presented, and an imaging flow cytometer with high speed and high throughput is demonstrated experimentally. Finally, the limitations and potential applications of high-speed SPI are discussed.
基金supported by JSPS KAKENHI(20H00125,21K20500,and 23H00273),Research Foundation for Opto-Science and Technology,Nakatani FoundationUTEC-UTokyo FSI Research Grant Program.
文摘Raman scattering spectroscopy is widely used as an analytical technique in various fields,but its measurement process tends to be slow due to the low scattering cross-section.In the last decade,various broadband coherent Raman scattering spectroscopy techniques have been developed to address this limitation,achieving a measurement rate of 500 kSpectra/s.Here,we present a substantially increased measurement rate of 50 MSpectra/s,which is 100 times higher than the previous state-of-the-art,by developing time-stretch coherent Raman scattering spectroscopy.Our newly developed system,based on a mode-locked Yb fiber laser,enables highly efficient broadband excitation of molecular vibrations via impulsive stimulated Raman scattering with an ultrashort femtosecond pulse and sensitive time-stretch detection with a picosecond probe pulse at a high repetition rate of the laser.As a proof-of-concept demonstration,we measure broadband coherent Stokes Raman scattering spectra of organic compounds covering the molecular fingerprint region from 200 to 1,200 cm^(-1).This high-speed broadband vibrational spectroscopy technique holds promise for unprecedented measurements of sub-microsecond dynamics of irreversible phenomena and extremely high-throughput measurements.
