Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary sa...Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary salt intake,and diets deficient in vegetables and fruits.Due to the often subtle and nonspecific early symptoms,coupled with the lack of routine screening programs,a significant proportion of GC cases are diagnosed at advanced stages.The etiology of GC is multifactorial,and diagnosis is confirmed histologically through endoscopic biopsy,followed by staging via computed tomography,positron emission tomography,staging laparoscopy,and endoscopic ultrasound.Treatment strategies typically involve a multidisciplinary approach including chemotherapy,surgical resection,radiotherapy,and emerging immunotherapeutic options.Despite advances in diagnostic and therapeutic modalities,the prognosis of advanced GC remains poor,with high rates of recurrence and metastasis.In recent years,increasing attention has been given to the role of tight junction(TJ)proteins in the pathogenesis and progression of GC.TJ proteins,critical components of epithelial barrier function,have been implicated in various stages of gastric carcinogenesis,from intestinal metaplasia to invasion and metastasis.Infection and inflammation,particularly due to Helicobacter pylori,disrupt TJ integrity,compromising the gastric mucosal barrier and facilitating neoplastic transformation.This review synthesizes current evidence from PubMed,EMBASE,Google Scholar,ScienceDirect,SpringerLink,and other reputable databases to provide a comprehensive overview of the involvement of TJ proteins in GC.By elucidating the molecular interplay between TJ dysregulation and gastric tumorigenesis,this work aims to highlight the potential of TJ proteins as novel diagnostic biomarkers and therapeutic targets in GC management.展开更多
The blood-spinal cord barrier is crucial for preserving homeostasis of the central nervous system.After spinal cord injury,autophagic flux within endothelial cells is disrupted,compromising the integrity of the blood-...The blood-spinal cord barrier is crucial for preserving homeostasis of the central nervous system.After spinal cord injury,autophagic flux within endothelial cells is disrupted,compromising the integrity of the blood-spinal cord barrier.This disruption facilitates extensive infiltration of inflammatory cells,resulting in exacerbated neuroinflammatory responses,neuronal death,and impaired neuronal regeneration.Previous research has demonstrated that photobiomodulation promotes the regeneration of damaged nerves following spinal cord injury by inhibiting the recruitment of inflammatory cells to the injured site and restoring neuronal mitochondrial function.However,the precise mechanisms by which photobiomodulation regulates neuroinflammation remain incompletely elucidated.In this study,we established a mouse model of spinal cord injury and assessed the effects of photobiomodulation treatment.Photobiomodulation effectively cleared damaged mitochondria from endothelial cells in mice,promoting recovery of hindlimb motor function.Using microvascular endothelial bEnd.3 cells subjected to oxygen-glucose deprivation,we found that the effects of photobiomodulation were mediated through activation of the PINK1/Parkin pathway.Additionally,photobiomodulation reduced mitochondrial oxidative stress levels and increased the expression of tight junction proteins within the blood-spinal cord barrier.Our findings suggest that photobiomodulation activates mitochondrial autophagy in endothelial cells through the PINK1/Parkin pathway,thereby promoting repair of the blood-spinal cord barrier following spinal cord injury.展开更多
Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-...Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-mediated e–h recombination the dominant decay pathway.In this work,nonradiative e–h recombination within excitons in monolayer MoS2 is investigated using first-principles simulations that combine nonadiabatic molecular dynamics with𝐺𝑊and real-time Bethe–Salpeter equation(BSE)propagation.A two-step process is identified:rapid intervalley redistribution induced by exchange interaction,followed by slower phonon-assisted recombination facilitated by exciton binding.By selectively removing the screened Coulomb and exchange terms from the BSE Hamiltonian,their respective contributions are disentangled—exchange interaction is found to increase the number of accessible recombination pathways,while binding reduces the excitation energy and enhances nonradiative decay.A reduction in recombination lifetime by over an order of magnitude is observed due to the excitonic many-body effects.These findings provide microscopic insights for understanding and tuning exciton lifetimes in 2D transition-metal dichalcogenides.展开更多
The physical properties of hydrocarbon reservoirs are important factors affecting the percolation ability of the reservoirs.Tight-sand reservoirs exhibit complex pore throat connectivity due to the extensive developme...The physical properties of hydrocarbon reservoirs are important factors affecting the percolation ability of the reservoirs.Tight-sand reservoirs exhibit complex pore throat connectivity due to the extensive development of micro-and nano-scale pore and throat systems.Characterizing the microscopic properties of these reservoirs using nondestructive,quantitative methods serves as an important means to determine the characteristics of microscopic pores and throats in tight-sand reservoirs and the mechanism behind the influence of these characteristics on reservoir porosity and permeability.In this study,a low-permeability sandstone sample and two tight sandstone samples collected from the Ordos Basin were nondestructively tested using high-resolution nano-CT technology to quantitively characterize their microscopic pore throat structures and model them three-dimensionally(in 3D)based on CT threshold differences and gray models.A thorough analysis and comparison reveal that the three samples exhibit a certain positive correlation between their porosity and permeability but the most important factor affecting both porosity and permeability is the microscopic pore throat structure.Although the number of pores in tight sandstones shows a minor impact on their porosity,large pores(more than 20μm)contribute predominantly to porosity,suggesting that the permeability of tight sandstones is controlled primarily by large pore throats.For these samples,higher permeability corresponds to larger average throat sizes.Therefore,throats with average radii greater than 2μm can significantly improve the permeability of tight sandstones.展开更多
The tight sandstone reservoirs in the first sub-member of Chang 7 member(Chang 71)of Triassic Yanchang Formation in the Jiyuan area,Ordos Basin,show significant variations in microscopic pore-throat structure(PTS)and ...The tight sandstone reservoirs in the first sub-member of Chang 7 member(Chang 71)of Triassic Yanchang Formation in the Jiyuan area,Ordos Basin,show significant variations in microscopic pore-throat structure(PTS)and fluid mobility due to the influences of the northeast and northwest dual provenance systems.This study performed multiple experimental analyses on nine samples from the area to determine the petrological and petrophysical properties,as well as the PTS characteristics of reservoirs in different provenance-controlled regions.On this basis,the pore-throat size distribution(PSD)obtained from high-pressure mercury injection(HPMI)was utilized to convert the NMR movable fluid T2spectrum,allowing for quantitative characterization of the full PSD and the occurrence characteristics of movable fluids.A systematic analysis was conducted on the primary controlling factors affecting fluid mobility in the reservoir.The results indicated that the lithology in the eastern and western regions is lithic arkose.The eastern sandstones,being farther from the provenance,exhibit higher contents of feldspar and lithic fragments,along with the development of more dissolution pores.The reservoir possesses good petrophysical properties,low displacement pressure,and high pore-throat connectivity and homogeneity,indicating strong fluid mobility.In contrast,the western sandstones,being nearer to the provenance,exhibit poor grain sorting,high contents of lithic fragments,strong compaction and cementation effects,resulting in poor petrophysical properties,and strong pore-throat heterogeneity,revealing weak fluid mobility.The range of full PSD in the eastern reservoir is wider than that in the western reservoir,with relatively well-developed macropores.The macropores are the primary space for occurrence of movable fluids,and controls the fluid mobility of the reservoir.The effective porosity of movable fluids(EPMF)quantitatively represents the pore space occupied by movable fluids within the reservoir and correlates well with porosity,permeability,and PTS parameters,making it a valuable parameter for evaluating fluid mobility.Under the multi-provenance system,the eastern and western reservoirs underwent different sedimentation and diagenesis processes,resulting in differential distribution of reservoir mineral components and pore types,which in turn affects the PTS heterogeneity and reservoir quality.The composition and content of reservoir minerals are intrinsic factors influencing fluid mobility,while the microscopic PTS is the primary factor controlling it.Low clay mineral content,welldeveloped macropores,and weak pore-throat heterogeneity all contribute to the storage and seepage of reservoir fluids.展开更多
Supercritical CO_(2)(SC-CO_(2))fracturing stands out a promising waterless stimulation technique in the development of unconventional resources.While numerous studies have delved into the inducedfracture mechanism of ...Supercritical CO_(2)(SC-CO_(2))fracturing stands out a promising waterless stimulation technique in the development of unconventional resources.While numerous studies have delved into the inducedfracture mechanism of SC-CO_(2),the small scale of rock samples and synthetic materials used in many studies have limited a comprehensive understanding of fracture propagation in unconventional formations.In this study,cubic tight sandstone samples with dimensions of 300 mm were employed to conduct SC-CO_(2)fractu ring experiments under true-triaxial stre ss conditions.The spatial morphology and quantitative attributes of fracture induced by water and SC-CO_(2)fracturing were compared,while the impact of in-situ stress on fracture propagation was also investigated.The results indicate that the SCCO_(2)fracturing takes approximately ten times longer than water fracturing.Furthermore,under identical stress condition,the breakdown pressure(BP)for SC-CO_(2)fracturing is nearly 25%lower than that for water fracturing.A quantitative analysis of fracture morphology reveals that water fracturing typically produces relatively simple fracture pattern,with the primary fracture distribution predominantly controlled by bedding planes.In contrast,SC-CO_(2)fracturing results in a more complex fracture morphology.As the differential of horizontal principal stress increases,the BP for SC-CO_(2)fractured rock exhibits a downward trend,and the induced fracture morphology becomes more simplified.Moreover,the presence of abnormal in-situ stress leads to a further increase in the BP for SC-CO_(2)fracturing,simultaneously enhancing the development of a more conductive fracture network.These findings provide critical insights into the efficiency and behavior of SC-CO_(2)fracturing in comparison to traditional water-based fracturing,offering valuable implication for its potential applications in unconventional reservoirs.展开更多
Understanding the storage mechanisms in CO_(2)flooding is crucial,as many carbon capture,utilization,and storage(CCUS)projects are related to enhanced oil recovery(EOR).CO_(2)storage in reservoirs across large timesca...Understanding the storage mechanisms in CO_(2)flooding is crucial,as many carbon capture,utilization,and storage(CCUS)projects are related to enhanced oil recovery(EOR).CO_(2)storage in reservoirs across large timescales undergoes the two storage stages of oil displacement and well shut-in,which cover mul-tiple replacement processes of injection-production synchronization,injection only with no production,and injection-production stoppage.Because the controlling mechanism of CO_(2)storage in different stages is unknown,the evolution of CO_(2)storage mechanisms over large timescales is not understood.A math-ematical model for the evaluation of CO_(2)storage,including stratigraphic,residual,solubility,and mineral trapping in low-permeability tight sandstone reservoirs,was established using experimental and theoret-ical analyses.Based on a detailed geological model of the Huaziping Oilfield,calibrated with reservoir permeability and fracture characteristic parameters obtained from well test results,a dynamic simulation of CO_(2)storage for the entire reservoir life cycle under two scenarios of continuous injection and water-gas alternation were considered.The results show that CO_(2)storage exhibits the significant stage charac-teristics of complete storage,dynamic storage,and stable storage.The CO_(2)storage capacity and storage rate under the continuous gas injection scenario(scenario 1)were 6.34×10^(4)t and 61%,while those under the water-gas alternation scenario(scenario 2)were 4.62×10^(4)t and 46%.The proportions of stor-age capacity under scenarios 1 and 2 for structural or stratigraphic,residual,solubility,and mineral trap-ping were 33.36%,33.96%,32.43%,and 0.25%;and 15.09%,38.65%,45.77%,and 0.49%,respectively.The evolution of the CO_(2)storage mechanism showed an overall trend:stratigraphic and residual trapping first increased and then decreased,whereas solubility trapping gradually decreased,and mineral trapping continuously increased.Based on these results,an evolution diagram of the CO_(2)storage mechanism of low-permeability tight sandstone reservoirs across large timescales was established.展开更多
Coal measures are significant hydrocarbon source rocks and reservoirs in petroliferous basins.Many large gas fields and coalbed methane fields globally are originated from coal-measure source rocks or accumulated in c...Coal measures are significant hydrocarbon source rocks and reservoirs in petroliferous basins.Many large gas fields and coalbed methane fields globally are originated from coal-measure source rocks or accumulated in coal rocks.Inspired by the discovery of shale oil and gas,and guided by“the overall exploration concept of considering coal rock as reservoir”,breakthroughs in the exploration and development of coal-rock gas have been achieved in deep coal seams with favorable preservation conditions,thereby opening up a new development frontier for the unconventional gas in coal-rock reservoirs.Based on the data from exploration and development practices,a systematic study on the accumulation mechanism of coal-rock gas has been conducted.The mechanisms of“three fields”controlling coal-rock gas accumulation are revealed.It is confirmed that the coal-rock gas is different from CBM in accumulation process.The whole petroleum systems in the Carboniferous–Permian transitional facies coal measures of the eastern margin of the Ordos Basin and in the Jurassic continental facies coal measures of the Junggar Basin are characterized,and the key research directions for further developing the whole petroleum system theory of coal measures are proposed.Coal rocks,compared to shale,possess intense hydrocarbon generation potential,strong adsorption capacity,dual-medium reservoir properties,and partial or weak oil and gas self-sealing capacity.Additionally,unlike other unconventional gas such as shale gas and tight gas,coal-rock gas exhibits more complex accumulation characteristics,and its accumulation requires a certain coal-rock play form lithological and structural traps.Coal-rock gas also has the characteristics of conventional fractured gas reservoirs.Compared with the basic theory and model of the whole petroleum system established based on detrital rock formations,coal measures have distinct characteristics and differences in coal-rock reservoirs and source-reservoir coupling.The whole petroleum system of coal measures is composed of various types of coal-measure hydrocarbon plays with coal(and dark shale)in coal measures as source rock and reservoir,and with adjacent tight layers as reservoirs or cap or transport layers.Under the action of source-reservoir coupling,coal-rock gas is accumulated in coal-rock reservoirs with good preservation conditions,tight oil/gas is accumulated in tight layers,conventional oil/gas is accumulated in traps far away from sources,and coalbed methane is accumulated in coal-rock reservoirs damaged by later geological processes.The proposed whole petroleum system of coal measures represents a novel type of whole petroleum system.展开更多
Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was ...Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was conducted on the structural characteristics and evolution,reservoir diagenesis and densification processes,and types and stages of faults/fractures,and revealing the multi-stage and multi-factor dynamic coupled enrichment mechanisms of tight gas reservoirs.(1)In the early Yanshan period,the paleo-structural traps were formed with low-medium maturity hydrocarbons accumulating in structural highs driven by buoyancy since reservoirs were not fully densified in this stage,demonstrating paleo-structure control on traps and early hydrocarbon accumulation.(2)In the middle-late Yanshan period,the source rocks became mature to generate and expel a large quantity of hydrocarbons.Grain size and type of sandstone controlled the time of reservoir densification,which restricted the scale of hydrocarbon charging,allowing for only a small-scale migration through sand bodies near the fault/fracture or less-densified matrix reservoirs.(3)During the Himalayan period,the source rocks reached overmaturity,and the residual oil cracking gas was efficiently transported along the late-stage faults/fractures.Wells with high production capacity were mainly located in Type I and II fault/fracture zones comprising the late-stage north-south trending fourth-order faults and the late-stage fractures.The productivity of the wells was controlled by the transformation of the late-stage faults/fractures.(4)The Xinchang structural belt underwent three stages of tectonic evolution,two stages of reservoir formation,and three stages of fault/fractures development.Hydrocarbons mainly accumulated in the paleo-structure highs.After reservoir densification and late fault/fracture adjustment,a complex gas-water distribution pattern was formed.Thus,it is summarized as the model of“near-source and low-abundance hydrocarbon charging in the early stage,and differential enrichment of natural gas under the joint control of fault-fold-fracture complex,high-quality reservoirs and structural highs in the late stage”.Faults/fractures with well-coupled fault-fold-fracture-pore are favorable exploration targets with high exploration effectiveness.展开更多
The Triassic Xujiahe Formation in the slope zone of the Sichuan foreland basin is a new field of continental tight gas exploration in recent years.The fourth member of the Xujiahe Formation(Xu4 Member),the major inter...The Triassic Xujiahe Formation in the slope zone of the Sichuan foreland basin is a new field of continental tight gas exploration in recent years.The fourth member of the Xujiahe Formation(Xu4 Member),the major interval in the Jianyang Block of the Tianfu gas field in the basin,is characterized by considerable buried depth,tight reservoirs,and strong heterogeneity.By using cast thin section,X-ray diffraction(XRD),scanning electron microscopy(SEM),fluid inclusion thermometry,and core analysis,the reservoir rock types,dominant diageneses,diagenetic history,and controls on high-graded reservoirs were investigated.It is found that the Xu4 Member in Jianyang mainly consists of lithic feldspar sandstones and feldspar lithic sandstones,followed by lithic quartz sandstones.High-energy hydrodynamic conditions in the microfacies of underwater distributary channels and mouth bars are beneficial to the preservation of primary pores and the occurrence of secondary pores,and there are no significant differences in petrophysical properties between these two microfacies.Compaction and calcareous cementation are the dominant controls on reservoir porosity decrease in the Xujiahe Formation;corrosion is the major contributor to porosity increase by generating secondary dissolved pores,e.g.intragranular dissolved pores and intergranular dissolved pores,as major reservoir space in the study area.Fracture zones around the faults inside the Xujiahe Formation(fourth‒order faults)are favorable for proximal tight gas accumulation,preservation,and production.The research findings have been successfully applied to explore the Xujiahe Formation in the slope zone of the Sichuan foreland basin.They can be referential for other similar tight sandstone gas accumulations.展开更多
In order to identify the development characteristics of fracture network in tight conglomerate reservoir of Mahu after hydraulic fracturing,a hydraulic fracturing test site was set up in the second and third members o...In order to identify the development characteristics of fracture network in tight conglomerate reservoir of Mahu after hydraulic fracturing,a hydraulic fracturing test site was set up in the second and third members of Triassic Baikouquan Formation(T1b2 and T1b3)in Ma-131 well area,which learned from the successful experience of hydraulic fracturing test sites in North America(HFTS-1).Twelve horizontal wells and a high-angle coring well MaJ02 were drilled.The orientation,connection,propagation law and major controlling factors of hydraulic fractures were analyzed by comparing results of CT scans,imaging logs,direct observation of cores from Well MaJ02,and combined with tracer monitoring data.Results indicate that:(1)Two types of fractures have developed by hydraulic fracturing,i.e.tensile fractures and shear fractures.Tensile fractures are approximately parallel to the direction of the maximum horizontal principal stress,and propagate less than 50 m from perforation clusters.Shear fractures are distributed among tensile fractures and mainly in the strike-slip mode due to the induced stress field among tensile fractures,and some of them are in conjugated pairs.Overall,tensile fractures alternate with shear fractures,with shear fractures dominated and activated after tensile ones.(2)Tracer monitoring results indicate that communication between wells was prevalent in the early stage of production,and the static pressure in the fracture gradually decreased and the connectivity between wells reduced as production progressed.(3)Density of hydraulic fractures is mainly affected by the lithology and fracturing parameters,which is smaller in the mudstone than the conglomerate.Larger fracturing scale and smaller cluster spacing lead to a higher fracture density,which are important directions to improve the well productivity.展开更多
Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and ...Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.展开更多
This article presents a case study of a 20-year-old male patient diagnosed with dilated cardiomyopathy(DCM)(NYHA IV).This condition was diagnosed as"heart failure disease"(water overflowing due to yang defic...This article presents a case study of a 20-year-old male patient diagnosed with dilated cardiomyopathy(DCM)(NYHA IV).This condition was diagnosed as"heart failure disease"(water overflowing due to yang deficiency,intermingled phlegm and stasis)in traditional Chinese medicine(TCM).The treatment approach employed a combination of TCM and Western medicine.Western medicine involved the administration of sacubitril valsartan sodium tablets to inhibit ventricular remodeling,in conjunction with diuretics and cardiotonic agents.Initially,TCM utilized a static infusion of Shenfu injection,which was subsequently supplemented with Qiliqiangxin capsules to invigorate qi,warm yang,activate blood circulation,and promote diuresis.After a follow-up period of 3 years,the patient's ejection fraction(EF)improved from 23%to 51%,and the left ventricular end diastolic diameter(LVed)decreased from 68 to 52 mm,accompanied by a significant alleviation of symptoms.These findings indicate that the combined treatment of TCM and Western medicine can synergistically enhance cardiac function and impede the progression of the disease,thereby offering valuable insights for the optimal management of DCM.展开更多
In contrast to conventional reservoirs,tight formations have more complex pore structures and significant boundary layer effect,making it difficult to determine the effective permeability.To address this,this paper fi...In contrast to conventional reservoirs,tight formations have more complex pore structures and significant boundary layer effect,making it difficult to determine the effective permeability.To address this,this paper first proposes a semi-empirical model for calculating boundary layer thickness based on dimensional analysis,using published experimental data on microcapillary flow.Furthermore,considering the non-uniform distribution of fluid viscosity in the flow channels of tight reservoirs,a theoretical model for boundary layer thickness is established based on fractal theory,and permeability predictions are conducted through Monte Carlo simulations.Finally,sensitivity analyses of various influencing parameters are performed.The results show that,compared to other fractal-based analytical models,the proposed permeability probabilistic model integrates parameters affecting fluid flow with random numbers,reflecting both the fractal and randomness characteristics of capillary size distribution.The computational results exhibit the highest consistency with experimental data.Among the factors affecting the boundary layer,in addition to certain conventional physical and mechanical parameters,different microstructure parameters significantly influence the boundary layer as well.A higher tortuosity fractal dimension results in a thicker boundary layer,while increases in pore fractal dimension,porosity,and maximum capillary size help mitigate the boundary layer effect.It is also observed that the permeability of large pores exhibits greater sensitivity to changes in various influencing parameters.Considering micro-scale flow effects,the proposed model enhances the understanding of the physical mechanisms of fluid transport in dense porous media.展开更多
The Double Take column looks at a single topic from an African and Chinese perspective.This month,we discuss the concept of travel with a small budget.The concept of Qiongyou-travelling on a tight budget-has gained si...The Double Take column looks at a single topic from an African and Chinese perspective.This month,we discuss the concept of travel with a small budget.The concept of Qiongyou-travelling on a tight budget-has gained significant traction among young travellers in China.展开更多
This study constructed an in vitro blood-brain barrier(BBB)transwell model to investigate the regulatory effects and mechanisms of the photothermal effects of gold nanorods(AuNRs)excited by the second near-infrared re...This study constructed an in vitro blood-brain barrier(BBB)transwell model to investigate the regulatory effects and mechanisms of the photothermal effects of gold nanorods(AuNRs)excited by the second near-infrared region(NIR-II)on BBB permeability.The experimental results showed that the photothermal effects of NIR-II t AuNRs significantly decreased trans-epithelial electrical resistance(TEER)and increased the permeability of fluorescein isothiocyanate(FITC)-dextran,indicating that it can effectively open the BBB.This effect was reversible,and the TEER and FITC permeability returned to baseline levels within 24 h after treatment.Mechanistic studies revealed that BBB opening did not rely on apoptosis,cytoskeletal disruption,mitochondrial dysfunction,or inflammation.The opening of the BBB was closely associated with a temporary decrease in the expression and conformational change of the tight junction protein occludin due to the photothermal effect.Molecular simulations and docking analysis revealed that the heat shock protein HSP70 could bind to the conformationally altered occludin,supporting the regulatory role of photothermal effects on tight junction proteins.In summary,NIR-II t AuNRs achieved safe and reversible opening of the BBB by regulating the conformation and expression of tight junction proteins,providing a deeper insight for further research on BBB and the treatment of neurological diseases.展开更多
Tight oil is the most viable target for unconventional oil and gas exploration, but the complexity of micro-/nanopore throat systems significantly affects the oil content of reservoirs. To investigate the causes of he...Tight oil is the most viable target for unconventional oil and gas exploration, but the complexity of micro-/nanopore throat systems significantly affects the oil content of reservoirs. To investigate the causes of heterogeneity in oil-bearing reservoirs, a high-pressure mercury injection experiment combined with fractal theory was conducted to analyze the micro pore throat structure characteristics of the tight sandstone of Chang 7 Member reservoirs in the Ordos Basin. The factors controlling the variations in oil content among tight sandstone samples were identified based on mineral composition characteristics. The results indicate that the pore throat radius distribution is mainly unimodal an bimodal. In oil-bearing samples, the pore throat distributions align well with the corresponding permeability contribution curves, while in oil-free samples, there is a clear deviation from these curves. Mesopore throats exert the greatest influence on seepage capacity. Differences in fractal characteristics are primarily reflected in D1 values, with oil-free samples exhibiting D1 values close to 3, indicating an extremely nonuniform pore throat structure at this scale. The content of quartz, plagioclase, and chlorite is significantly higher in oil-bearing samples than in oil-free samples, whereas calcite content is lower in oil-bearing samples. There is a positive correlation between the contents of quartz, plagioclase, and chlorite with D1;their increased presence contributes to a more favorable pore throat structure.Conversely, the calcite contents show an inverse relationship with D1. Cementation increases the complexity of pore throat structures, while multiple diagenetic processes simultaneously control these characteristics, leading to variations in oil content.展开更多
In the context of complex tectonic evolution,due to the control of tectonic compression stress and faults on tectonic fractures,the formation and development of tectonic fractures in the T_3x~2 tight reservoirs presen...In the context of complex tectonic evolution,due to the control of tectonic compression stress and faults on tectonic fractures,the formation and development of tectonic fractures in the T_3x~2 tight reservoirs present significant variations across different tectonic segments in the Western Sichuan Foreland Basin.We clarified the control of differential tectonic evolution on the formation and development of tectonic fractures in different tectonic segments through field-based observations,core samples,image logging,as well as fluid inclusion petrography and temperature determinations of fracture-filling materials,combined with 2D balanced cross-section restoration.The study area primarily manifests two types of tectonic fractures in the tight reservoirs:orogen-related fractures(regional fractures)and fault-related fractures.The orientations of these fractures are predominantly E-W,nearly N-S,NE,and NW.Specifically,the northern segment area only shows the development of regional fractures,while the southern and middle segments exhibit the development of both regional and tectonic fractures.There are three phases of tectonic fractures in different tectonic segments,and their formation times are relatively consistent.The Mesozoic tectonic events had a significant impact on the northern and central segments,with the amount of tectonic shortening and the rate of stratigraphic shortening gradually decreasing from the northeast to the southwest.The compressional stress resulting from tectonic compression also decreases from the northeast to the southwest.As a result,the development of first-phase and second-phase tectonic shear fractures is more pronounced in the northern and middle segments compared to the southern segment.Under the significant control of faults,the development of N-S-and NE-oriented fault-related fractures is more pronounced in the southern segment,while the development of NE-oriented fault-related fractures is relatively higher in the middle segment.Overall,there is an increased density of fractures and an increasing trend in fracture scale from the northern to the middle and then to the southern segment.展开更多
Accurately predicting relative permeability is an important issue in the research of multiphase flow in tight reservoirs.Existing predictive models typically rely on the capillary tube bundle model featuring circular ...Accurately predicting relative permeability is an important issue in the research of multiphase flow in tight reservoirs.Existing predictive models typically rely on the capillary tube bundle model featuring circular cross-sections,often overlooking the impact of pore geometry on fluid flow behavior within reservoirs.In this work,the intermingled fractal theory of porous media is introduced to characterize the intricate local features within the internal space of tight rocks.Initially,iterative rules for diverse fractal units are skillfully designed to capture the actual characteristics of pore cross-sectional shapes.Subsequently,analytical relationships are derived between the iterative parameters and the area,wetted perimeter,and hydraulic diameter of pores generated by these units,followed by the establishment of a relative permeability model that considers pore geometry.The model's validity is confirmed through comparisons with experimental data and published relative permeability models,with correlation coefficients exceeding 0.996.Finally,various factors affecting two-phase flow characteristics are analyzed.The results reveal that pore geometry has a significant impact on flow behavior in porous media.Assuming that the flow channels are cylindrical typically leads to an overestimation of permeability,with the maximum relative error reaching 46.91%.Additionally,the tortuosity fractal dimension is a determinant factor influencing the relative permeability of both wetting and nonwetting fluids,and the phase permeability is sensitive to variations in solid particle size and porosity.The proposed intermingled fractal model enhances the accuracy of evaluating fluid flow characteristics in microscale pore channels and offers a novel framework for simulating porous media with complex geometries.展开更多
Tight sandstone has become an important area in gas exploration.In this study,we propose a 3D seismic reservoir parameter inversion method for tight gas-bearing sandstone reservoirs using dual neural networks.The firs...Tight sandstone has become an important area in gas exploration.In this study,we propose a 3D seismic reservoir parameter inversion method for tight gas-bearing sandstone reservoirs using dual neural networks.The first network referred to as the inversion network,receives seismic data and predicts reservoir parameters.At well locations,these predictions will be validated based on actual reservoir parameters to evaluate errors.For non-well locations,synthetic seismic data are generated by the application of rock physics forward modeling and seismic reflection coefficient equations.The errors are then calculated by comparing synthetic seismic data with actual seismic data.During the rock physics forward modeling,pseudo reservoir parameters are derived by perturbing the actual reservoir parameters,which are then used to generate pseudo elastic parameters through the modeling.Both the actual and pseudo parameters are then used to train the second network,referred to as the rock physics network.By incorporating the rock physics network,the method effectively alleviates issues such as gradient explosion that may arise from directly integrating rock physics computations into the network,while the inclusion of pseudo parameters enhances the network's generalization capability.The proposed method enables the direct inversion of porosity,clay conte nt,and water saturation from pre-stack seismic data using deep learning,thereby achieving quantitative predictions of reservoir rock physical parameters.The application to the field data from tight sandstone gas reservoirs in southwestern China demonstrates the method has the good capability of indicating the gas-bearing areas and provide high resolution.展开更多
文摘Gastric cancer(GC)is the fifth most prevalent malignancy worldwide and remains a leading cause of cancer-related mortality.Major risk factors for GC include Helicobacter pylori infection,increasing age,high dietary salt intake,and diets deficient in vegetables and fruits.Due to the often subtle and nonspecific early symptoms,coupled with the lack of routine screening programs,a significant proportion of GC cases are diagnosed at advanced stages.The etiology of GC is multifactorial,and diagnosis is confirmed histologically through endoscopic biopsy,followed by staging via computed tomography,positron emission tomography,staging laparoscopy,and endoscopic ultrasound.Treatment strategies typically involve a multidisciplinary approach including chemotherapy,surgical resection,radiotherapy,and emerging immunotherapeutic options.Despite advances in diagnostic and therapeutic modalities,the prognosis of advanced GC remains poor,with high rates of recurrence and metastasis.In recent years,increasing attention has been given to the role of tight junction(TJ)proteins in the pathogenesis and progression of GC.TJ proteins,critical components of epithelial barrier function,have been implicated in various stages of gastric carcinogenesis,from intestinal metaplasia to invasion and metastasis.Infection and inflammation,particularly due to Helicobacter pylori,disrupt TJ integrity,compromising the gastric mucosal barrier and facilitating neoplastic transformation.This review synthesizes current evidence from PubMed,EMBASE,Google Scholar,ScienceDirect,SpringerLink,and other reputable databases to provide a comprehensive overview of the involvement of TJ proteins in GC.By elucidating the molecular interplay between TJ dysregulation and gastric tumorigenesis,this work aims to highlight the potential of TJ proteins as novel diagnostic biomarkers and therapeutic targets in GC management.
基金supported by the National Natural Science Foundation of China,No.82471411(to ZW and TD)the Key Research and DevelopmentProgram of Shaanxi Province,No.2023-ZDLSF-12(to TD).
文摘The blood-spinal cord barrier is crucial for preserving homeostasis of the central nervous system.After spinal cord injury,autophagic flux within endothelial cells is disrupted,compromising the integrity of the blood-spinal cord barrier.This disruption facilitates extensive infiltration of inflammatory cells,resulting in exacerbated neuroinflammatory responses,neuronal death,and impaired neuronal regeneration.Previous research has demonstrated that photobiomodulation promotes the regeneration of damaged nerves following spinal cord injury by inhibiting the recruitment of inflammatory cells to the injured site and restoring neuronal mitochondrial function.However,the precise mechanisms by which photobiomodulation regulates neuroinflammation remain incompletely elucidated.In this study,we established a mouse model of spinal cord injury and assessed the effects of photobiomodulation treatment.Photobiomodulation effectively cleared damaged mitochondria from endothelial cells in mice,promoting recovery of hindlimb motor function.Using microvascular endothelial bEnd.3 cells subjected to oxygen-glucose deprivation,we found that the effects of photobiomodulation were mediated through activation of the PINK1/Parkin pathway.Additionally,photobiomodulation reduced mitochondrial oxidative stress levels and increased the expression of tight junction proteins within the blood-spinal cord barrier.Our findings suggest that photobiomodulation activates mitochondrial autophagy in endothelial cells through the PINK1/Parkin pathway,thereby promoting repair of the blood-spinal cord barrier following spinal cord injury.
基金supported by the National Key Research and Development Program of China (Grant Nos.2024YFA1409800 for J.Z.and2024YFA1408603 for Q.Z.)the National Natural Science Foundation of China (Grant Nos.12125408,12334004for J.Z.,and 12174363 for Q.Z.)+1 种基金the Innovation Program for Quantum Science and Technology (Grant No.2021ZD0303306 for J.Z.)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB0450101 for J.Z.)。
文摘Electron–hole(e–h)recombination is a fundamental process that governs energy dissipation and device efficiency in semiconductors.In two-dimensional(2D)materials,the formation of tightly bound excitons makes exciton-mediated e–h recombination the dominant decay pathway.In this work,nonradiative e–h recombination within excitons in monolayer MoS2 is investigated using first-principles simulations that combine nonadiabatic molecular dynamics with𝐺𝑊and real-time Bethe–Salpeter equation(BSE)propagation.A two-step process is identified:rapid intervalley redistribution induced by exchange interaction,followed by slower phonon-assisted recombination facilitated by exciton binding.By selectively removing the screened Coulomb and exchange terms from the BSE Hamiltonian,their respective contributions are disentangled—exchange interaction is found to increase the number of accessible recombination pathways,while binding reduces the excitation energy and enhances nonradiative decay.A reduction in recombination lifetime by over an order of magnitude is observed due to the excitonic many-body effects.These findings provide microscopic insights for understanding and tuning exciton lifetimes in 2D transition-metal dichalcogenides.
文摘The physical properties of hydrocarbon reservoirs are important factors affecting the percolation ability of the reservoirs.Tight-sand reservoirs exhibit complex pore throat connectivity due to the extensive development of micro-and nano-scale pore and throat systems.Characterizing the microscopic properties of these reservoirs using nondestructive,quantitative methods serves as an important means to determine the characteristics of microscopic pores and throats in tight-sand reservoirs and the mechanism behind the influence of these characteristics on reservoir porosity and permeability.In this study,a low-permeability sandstone sample and two tight sandstone samples collected from the Ordos Basin were nondestructively tested using high-resolution nano-CT technology to quantitively characterize their microscopic pore throat structures and model them three-dimensionally(in 3D)based on CT threshold differences and gray models.A thorough analysis and comparison reveal that the three samples exhibit a certain positive correlation between their porosity and permeability but the most important factor affecting both porosity and permeability is the microscopic pore throat structure.Although the number of pores in tight sandstones shows a minor impact on their porosity,large pores(more than 20μm)contribute predominantly to porosity,suggesting that the permeability of tight sandstones is controlled primarily by large pore throats.For these samples,higher permeability corresponds to larger average throat sizes.Therefore,throats with average radii greater than 2μm can significantly improve the permeability of tight sandstones.
文摘The tight sandstone reservoirs in the first sub-member of Chang 7 member(Chang 71)of Triassic Yanchang Formation in the Jiyuan area,Ordos Basin,show significant variations in microscopic pore-throat structure(PTS)and fluid mobility due to the influences of the northeast and northwest dual provenance systems.This study performed multiple experimental analyses on nine samples from the area to determine the petrological and petrophysical properties,as well as the PTS characteristics of reservoirs in different provenance-controlled regions.On this basis,the pore-throat size distribution(PSD)obtained from high-pressure mercury injection(HPMI)was utilized to convert the NMR movable fluid T2spectrum,allowing for quantitative characterization of the full PSD and the occurrence characteristics of movable fluids.A systematic analysis was conducted on the primary controlling factors affecting fluid mobility in the reservoir.The results indicated that the lithology in the eastern and western regions is lithic arkose.The eastern sandstones,being farther from the provenance,exhibit higher contents of feldspar and lithic fragments,along with the development of more dissolution pores.The reservoir possesses good petrophysical properties,low displacement pressure,and high pore-throat connectivity and homogeneity,indicating strong fluid mobility.In contrast,the western sandstones,being nearer to the provenance,exhibit poor grain sorting,high contents of lithic fragments,strong compaction and cementation effects,resulting in poor petrophysical properties,and strong pore-throat heterogeneity,revealing weak fluid mobility.The range of full PSD in the eastern reservoir is wider than that in the western reservoir,with relatively well-developed macropores.The macropores are the primary space for occurrence of movable fluids,and controls the fluid mobility of the reservoir.The effective porosity of movable fluids(EPMF)quantitatively represents the pore space occupied by movable fluids within the reservoir and correlates well with porosity,permeability,and PTS parameters,making it a valuable parameter for evaluating fluid mobility.Under the multi-provenance system,the eastern and western reservoirs underwent different sedimentation and diagenesis processes,resulting in differential distribution of reservoir mineral components and pore types,which in turn affects the PTS heterogeneity and reservoir quality.The composition and content of reservoir minerals are intrinsic factors influencing fluid mobility,while the microscopic PTS is the primary factor controlling it.Low clay mineral content,welldeveloped macropores,and weak pore-throat heterogeneity all contribute to the storage and seepage of reservoir fluids.
基金funded by the National Natural Scientific Foundation of China(Nos.52304008,52404038,52474043)the China Postdoctoral Science Foundation(No.2023MD734223)+1 种基金the Key Laboratory of Well Stability and Fluid&Rock Mechanics in Oil and Gas Reservoir of Shaanxi Province(No.23JS047)the Youth Talent Lifting Program of Xi'an Science and Technology Association(No.959202413078)。
文摘Supercritical CO_(2)(SC-CO_(2))fracturing stands out a promising waterless stimulation technique in the development of unconventional resources.While numerous studies have delved into the inducedfracture mechanism of SC-CO_(2),the small scale of rock samples and synthetic materials used in many studies have limited a comprehensive understanding of fracture propagation in unconventional formations.In this study,cubic tight sandstone samples with dimensions of 300 mm were employed to conduct SC-CO_(2)fractu ring experiments under true-triaxial stre ss conditions.The spatial morphology and quantitative attributes of fracture induced by water and SC-CO_(2)fracturing were compared,while the impact of in-situ stress on fracture propagation was also investigated.The results indicate that the SCCO_(2)fracturing takes approximately ten times longer than water fracturing.Furthermore,under identical stress condition,the breakdown pressure(BP)for SC-CO_(2)fracturing is nearly 25%lower than that for water fracturing.A quantitative analysis of fracture morphology reveals that water fracturing typically produces relatively simple fracture pattern,with the primary fracture distribution predominantly controlled by bedding planes.In contrast,SC-CO_(2)fracturing results in a more complex fracture morphology.As the differential of horizontal principal stress increases,the BP for SC-CO_(2)fractured rock exhibits a downward trend,and the induced fracture morphology becomes more simplified.Moreover,the presence of abnormal in-situ stress leads to a further increase in the BP for SC-CO_(2)fracturing,simultaneously enhancing the development of a more conductive fracture network.These findings provide critical insights into the efficiency and behavior of SC-CO_(2)fracturing in comparison to traditional water-based fracturing,offering valuable implication for its potential applications in unconventional reservoirs.
基金supported by the National Key Research and Development Program of China(2022YFE0206700).
文摘Understanding the storage mechanisms in CO_(2)flooding is crucial,as many carbon capture,utilization,and storage(CCUS)projects are related to enhanced oil recovery(EOR).CO_(2)storage in reservoirs across large timescales undergoes the two storage stages of oil displacement and well shut-in,which cover mul-tiple replacement processes of injection-production synchronization,injection only with no production,and injection-production stoppage.Because the controlling mechanism of CO_(2)storage in different stages is unknown,the evolution of CO_(2)storage mechanisms over large timescales is not understood.A math-ematical model for the evaluation of CO_(2)storage,including stratigraphic,residual,solubility,and mineral trapping in low-permeability tight sandstone reservoirs,was established using experimental and theoret-ical analyses.Based on a detailed geological model of the Huaziping Oilfield,calibrated with reservoir permeability and fracture characteristic parameters obtained from well test results,a dynamic simulation of CO_(2)storage for the entire reservoir life cycle under two scenarios of continuous injection and water-gas alternation were considered.The results show that CO_(2)storage exhibits the significant stage charac-teristics of complete storage,dynamic storage,and stable storage.The CO_(2)storage capacity and storage rate under the continuous gas injection scenario(scenario 1)were 6.34×10^(4)t and 61%,while those under the water-gas alternation scenario(scenario 2)were 4.62×10^(4)t and 46%.The proportions of stor-age capacity under scenarios 1 and 2 for structural or stratigraphic,residual,solubility,and mineral trap-ping were 33.36%,33.96%,32.43%,and 0.25%;and 15.09%,38.65%,45.77%,and 0.49%,respectively.The evolution of the CO_(2)storage mechanism showed an overall trend:stratigraphic and residual trapping first increased and then decreased,whereas solubility trapping gradually decreased,and mineral trapping continuously increased.Based on these results,an evolution diagram of the CO_(2)storage mechanism of low-permeability tight sandstone reservoirs across large timescales was established.
基金Supported by the PetroChina Basic Project(2024DJ23)CNPC Science Research and Technology Development Project(2021DJ0101)。
文摘Coal measures are significant hydrocarbon source rocks and reservoirs in petroliferous basins.Many large gas fields and coalbed methane fields globally are originated from coal-measure source rocks or accumulated in coal rocks.Inspired by the discovery of shale oil and gas,and guided by“the overall exploration concept of considering coal rock as reservoir”,breakthroughs in the exploration and development of coal-rock gas have been achieved in deep coal seams with favorable preservation conditions,thereby opening up a new development frontier for the unconventional gas in coal-rock reservoirs.Based on the data from exploration and development practices,a systematic study on the accumulation mechanism of coal-rock gas has been conducted.The mechanisms of“three fields”controlling coal-rock gas accumulation are revealed.It is confirmed that the coal-rock gas is different from CBM in accumulation process.The whole petroleum systems in the Carboniferous–Permian transitional facies coal measures of the eastern margin of the Ordos Basin and in the Jurassic continental facies coal measures of the Junggar Basin are characterized,and the key research directions for further developing the whole petroleum system theory of coal measures are proposed.Coal rocks,compared to shale,possess intense hydrocarbon generation potential,strong adsorption capacity,dual-medium reservoir properties,and partial or weak oil and gas self-sealing capacity.Additionally,unlike other unconventional gas such as shale gas and tight gas,coal-rock gas exhibits more complex accumulation characteristics,and its accumulation requires a certain coal-rock play form lithological and structural traps.Coal-rock gas also has the characteristics of conventional fractured gas reservoirs.Compared with the basic theory and model of the whole petroleum system established based on detrital rock formations,coal measures have distinct characteristics and differences in coal-rock reservoirs and source-reservoir coupling.The whole petroleum system of coal measures is composed of various types of coal-measure hydrocarbon plays with coal(and dark shale)in coal measures as source rock and reservoir,and with adjacent tight layers as reservoirs or cap or transport layers.Under the action of source-reservoir coupling,coal-rock gas is accumulated in coal-rock reservoirs with good preservation conditions,tight oil/gas is accumulated in tight layers,conventional oil/gas is accumulated in traps far away from sources,and coalbed methane is accumulated in coal-rock reservoirs damaged by later geological processes.The proposed whole petroleum system of coal measures represents a novel type of whole petroleum system.
基金Supported by the National Natural Science Foundation of China(42302141).
文摘Taking the second member of the Xujiahe Formation of the Upper Triassic in the Xinchang structural belt as an example,based on data such as logging,production,seismic interpretation and test,a systematic analysis was conducted on the structural characteristics and evolution,reservoir diagenesis and densification processes,and types and stages of faults/fractures,and revealing the multi-stage and multi-factor dynamic coupled enrichment mechanisms of tight gas reservoirs.(1)In the early Yanshan period,the paleo-structural traps were formed with low-medium maturity hydrocarbons accumulating in structural highs driven by buoyancy since reservoirs were not fully densified in this stage,demonstrating paleo-structure control on traps and early hydrocarbon accumulation.(2)In the middle-late Yanshan period,the source rocks became mature to generate and expel a large quantity of hydrocarbons.Grain size and type of sandstone controlled the time of reservoir densification,which restricted the scale of hydrocarbon charging,allowing for only a small-scale migration through sand bodies near the fault/fracture or less-densified matrix reservoirs.(3)During the Himalayan period,the source rocks reached overmaturity,and the residual oil cracking gas was efficiently transported along the late-stage faults/fractures.Wells with high production capacity were mainly located in Type I and II fault/fracture zones comprising the late-stage north-south trending fourth-order faults and the late-stage fractures.The productivity of the wells was controlled by the transformation of the late-stage faults/fractures.(4)The Xinchang structural belt underwent three stages of tectonic evolution,two stages of reservoir formation,and three stages of fault/fractures development.Hydrocarbons mainly accumulated in the paleo-structure highs.After reservoir densification and late fault/fracture adjustment,a complex gas-water distribution pattern was formed.Thus,it is summarized as the model of“near-source and low-abundance hydrocarbon charging in the early stage,and differential enrichment of natural gas under the joint control of fault-fold-fracture complex,high-quality reservoirs and structural highs in the late stage”.Faults/fractures with well-coupled fault-fold-fracture-pore are favorable exploration targets with high exploration effectiveness.
基金supported by the China National Petroleum Corporation Science and Technology Project(Study on genesis mechanism and distribution law of high quality reservoir of the fourth Member of Xujiahe Formation in middle and west Sichuan area,20230301-23)。
文摘The Triassic Xujiahe Formation in the slope zone of the Sichuan foreland basin is a new field of continental tight gas exploration in recent years.The fourth member of the Xujiahe Formation(Xu4 Member),the major interval in the Jianyang Block of the Tianfu gas field in the basin,is characterized by considerable buried depth,tight reservoirs,and strong heterogeneity.By using cast thin section,X-ray diffraction(XRD),scanning electron microscopy(SEM),fluid inclusion thermometry,and core analysis,the reservoir rock types,dominant diageneses,diagenetic history,and controls on high-graded reservoirs were investigated.It is found that the Xu4 Member in Jianyang mainly consists of lithic feldspar sandstones and feldspar lithic sandstones,followed by lithic quartz sandstones.High-energy hydrodynamic conditions in the microfacies of underwater distributary channels and mouth bars are beneficial to the preservation of primary pores and the occurrence of secondary pores,and there are no significant differences in petrophysical properties between these two microfacies.Compaction and calcareous cementation are the dominant controls on reservoir porosity decrease in the Xujiahe Formation;corrosion is the major contributor to porosity increase by generating secondary dissolved pores,e.g.intragranular dissolved pores and intergranular dissolved pores,as major reservoir space in the study area.Fracture zones around the faults inside the Xujiahe Formation(fourth‒order faults)are favorable for proximal tight gas accumulation,preservation,and production.The research findings have been successfully applied to explore the Xujiahe Formation in the slope zone of the Sichuan foreland basin.They can be referential for other similar tight sandstone gas accumulations.
基金Supported by the National Natural Science Foundation of China(52274051)CNPC-China University of Petroleum(Beijing)Strategic Cooperative Project(ZLZX2020-01).
文摘In order to identify the development characteristics of fracture network in tight conglomerate reservoir of Mahu after hydraulic fracturing,a hydraulic fracturing test site was set up in the second and third members of Triassic Baikouquan Formation(T1b2 and T1b3)in Ma-131 well area,which learned from the successful experience of hydraulic fracturing test sites in North America(HFTS-1).Twelve horizontal wells and a high-angle coring well MaJ02 were drilled.The orientation,connection,propagation law and major controlling factors of hydraulic fractures were analyzed by comparing results of CT scans,imaging logs,direct observation of cores from Well MaJ02,and combined with tracer monitoring data.Results indicate that:(1)Two types of fractures have developed by hydraulic fracturing,i.e.tensile fractures and shear fractures.Tensile fractures are approximately parallel to the direction of the maximum horizontal principal stress,and propagate less than 50 m from perforation clusters.Shear fractures are distributed among tensile fractures and mainly in the strike-slip mode due to the induced stress field among tensile fractures,and some of them are in conjugated pairs.Overall,tensile fractures alternate with shear fractures,with shear fractures dominated and activated after tensile ones.(2)Tracer monitoring results indicate that communication between wells was prevalent in the early stage of production,and the static pressure in the fracture gradually decreased and the connectivity between wells reduced as production progressed.(3)Density of hydraulic fractures is mainly affected by the lithology and fracturing parameters,which is smaller in the mudstone than the conglomerate.Larger fracturing scale and smaller cluster spacing lead to a higher fracture density,which are important directions to improve the well productivity.
基金supported by the National Natural Science Foundation of China(grants 31972908,81991500,81991502,and 32030010)Beijing Natural Science Foundation(grant 7202082).
文摘Tricellulin,a key tricellular tight junction(TJ)protein,is essential for maintaining the barrier integrity of acinar epithelia against macromolecular passage in salivary glands.This study aims to explore the role and regulatory mechanism of tricellulin in the development of salivary gland hypofunction in Sjögren’s syndrome(SS).Employing a multifaceted approach involving patient biopsies,non-obese diabetic(NOD)mice as a SS model,salivary gland acinar cell-specific tricellulin conditional knockout(TricCKO)mice,and IFN-γ-stimulated salivary gland epithelial cells,we investigated the role of tricellulin in SS-related hyposalivation.Our data revealed diminished levels of tricellulin in salivary glands of SS patients.Similarly,NOD mice displayed a reduction in tricellulin expression from the onset of the disease,concomitant with hyposecretion and an increase in salivary albumin content.Consistent with these findings,TricCKO mice exhibited both hyposecretion and leakage of macromolecular tracers when compared to control animals.Mechanistically,the JAK/STAT1/miR-145 axis was identified as mediating the IFN-γ-induced downregulation of tricellulin.Treatment with AT1001,a TJ sealer,ameliorated epithelial barrier dysfunction,restored tricellulin expression,and consequently alleviated hyposalivation in NOD mice.Importantly,treatment with miR-145 antagomir to specifically recover the expression of tricellulin in NOD mice significantly alleviated hyposalivation and macromolecular leakage.Collectively,we identified that tricellulin deficiency in salivary glands contributed to hyposalivation in SS.Our findings highlight tricellulin as a potential therapeutic target for hyposecretion,particularly in the context of reinforcing epithelial barrier function through preventing leakage of macromolecules in salivary glands.
文摘This article presents a case study of a 20-year-old male patient diagnosed with dilated cardiomyopathy(DCM)(NYHA IV).This condition was diagnosed as"heart failure disease"(water overflowing due to yang deficiency,intermingled phlegm and stasis)in traditional Chinese medicine(TCM).The treatment approach employed a combination of TCM and Western medicine.Western medicine involved the administration of sacubitril valsartan sodium tablets to inhibit ventricular remodeling,in conjunction with diuretics and cardiotonic agents.Initially,TCM utilized a static infusion of Shenfu injection,which was subsequently supplemented with Qiliqiangxin capsules to invigorate qi,warm yang,activate blood circulation,and promote diuresis.After a follow-up period of 3 years,the patient's ejection fraction(EF)improved from 23%to 51%,and the left ventricular end diastolic diameter(LVed)decreased from 68 to 52 mm,accompanied by a significant alleviation of symptoms.These findings indicate that the combined treatment of TCM and Western medicine can synergistically enhance cardiac function and impede the progression of the disease,thereby offering valuable insights for the optimal management of DCM.
基金supported by the Hebei Provincial Natural Science Foundation of China(No.D2023402012)the Major Science and Technology Project of China National Petroleum Corporation(No.2024DJ87).
文摘In contrast to conventional reservoirs,tight formations have more complex pore structures and significant boundary layer effect,making it difficult to determine the effective permeability.To address this,this paper first proposes a semi-empirical model for calculating boundary layer thickness based on dimensional analysis,using published experimental data on microcapillary flow.Furthermore,considering the non-uniform distribution of fluid viscosity in the flow channels of tight reservoirs,a theoretical model for boundary layer thickness is established based on fractal theory,and permeability predictions are conducted through Monte Carlo simulations.Finally,sensitivity analyses of various influencing parameters are performed.The results show that,compared to other fractal-based analytical models,the proposed permeability probabilistic model integrates parameters affecting fluid flow with random numbers,reflecting both the fractal and randomness characteristics of capillary size distribution.The computational results exhibit the highest consistency with experimental data.Among the factors affecting the boundary layer,in addition to certain conventional physical and mechanical parameters,different microstructure parameters significantly influence the boundary layer as well.A higher tortuosity fractal dimension results in a thicker boundary layer,while increases in pore fractal dimension,porosity,and maximum capillary size help mitigate the boundary layer effect.It is also observed that the permeability of large pores exhibits greater sensitivity to changes in various influencing parameters.Considering micro-scale flow effects,the proposed model enhances the understanding of the physical mechanisms of fluid transport in dense porous media.
文摘The Double Take column looks at a single topic from an African and Chinese perspective.This month,we discuss the concept of travel with a small budget.The concept of Qiongyou-travelling on a tight budget-has gained significant traction among young travellers in China.
基金supported by the Fundamental Research Funds for the Central Universities(No.DUT24YG142).
文摘This study constructed an in vitro blood-brain barrier(BBB)transwell model to investigate the regulatory effects and mechanisms of the photothermal effects of gold nanorods(AuNRs)excited by the second near-infrared region(NIR-II)on BBB permeability.The experimental results showed that the photothermal effects of NIR-II t AuNRs significantly decreased trans-epithelial electrical resistance(TEER)and increased the permeability of fluorescein isothiocyanate(FITC)-dextran,indicating that it can effectively open the BBB.This effect was reversible,and the TEER and FITC permeability returned to baseline levels within 24 h after treatment.Mechanistic studies revealed that BBB opening did not rely on apoptosis,cytoskeletal disruption,mitochondrial dysfunction,or inflammation.The opening of the BBB was closely associated with a temporary decrease in the expression and conformational change of the tight junction protein occludin due to the photothermal effect.Molecular simulations and docking analysis revealed that the heat shock protein HSP70 could bind to the conformationally altered occludin,supporting the regulatory role of photothermal effects on tight junction proteins.In summary,NIR-II t AuNRs achieved safe and reversible opening of the BBB by regulating the conformation and expression of tight junction proteins,providing a deeper insight for further research on BBB and the treatment of neurological diseases.
基金supported by the National Natural Science Foundation of China (Grant No. 42002139)the Basic Prospective Project of SINOPEC (Grant No. P23240-3)。
文摘Tight oil is the most viable target for unconventional oil and gas exploration, but the complexity of micro-/nanopore throat systems significantly affects the oil content of reservoirs. To investigate the causes of heterogeneity in oil-bearing reservoirs, a high-pressure mercury injection experiment combined with fractal theory was conducted to analyze the micro pore throat structure characteristics of the tight sandstone of Chang 7 Member reservoirs in the Ordos Basin. The factors controlling the variations in oil content among tight sandstone samples were identified based on mineral composition characteristics. The results indicate that the pore throat radius distribution is mainly unimodal an bimodal. In oil-bearing samples, the pore throat distributions align well with the corresponding permeability contribution curves, while in oil-free samples, there is a clear deviation from these curves. Mesopore throats exert the greatest influence on seepage capacity. Differences in fractal characteristics are primarily reflected in D1 values, with oil-free samples exhibiting D1 values close to 3, indicating an extremely nonuniform pore throat structure at this scale. The content of quartz, plagioclase, and chlorite is significantly higher in oil-bearing samples than in oil-free samples, whereas calcite content is lower in oil-bearing samples. There is a positive correlation between the contents of quartz, plagioclase, and chlorite with D1;their increased presence contributes to a more favorable pore throat structure.Conversely, the calcite contents show an inverse relationship with D1. Cementation increases the complexity of pore throat structures, while multiple diagenetic processes simultaneously control these characteristics, leading to variations in oil content.
基金financially supported by the National Natural Science Foundation of China(No.42402171)the China Postdoctoral Science Foundation(No.2023MD744255)+6 种基金the Natural Science Basic Research Program of Shaanxi(No.2024JC-YBQN-0353)the Scientific Research Program Funded by Shaanxi Provincial Education Department(No.23JK0600)the Shaanxi Postdoctoral Science Foundation(No.2023BSHEDZZ324)the project of Theory of Hydrocarbon Enrichment under Multi-Spheric Interactions of the Earth(No.THEMSIE04010107)the Key Research and Development Program of Shaanxi(No.2021KW-10)the Innovation Capability Support Program of Shaanxi(No.2022PT-08)the SINOPEC CCUS Fund Project(No.33550000-22ZC0613-0326)。
文摘In the context of complex tectonic evolution,due to the control of tectonic compression stress and faults on tectonic fractures,the formation and development of tectonic fractures in the T_3x~2 tight reservoirs present significant variations across different tectonic segments in the Western Sichuan Foreland Basin.We clarified the control of differential tectonic evolution on the formation and development of tectonic fractures in different tectonic segments through field-based observations,core samples,image logging,as well as fluid inclusion petrography and temperature determinations of fracture-filling materials,combined with 2D balanced cross-section restoration.The study area primarily manifests two types of tectonic fractures in the tight reservoirs:orogen-related fractures(regional fractures)and fault-related fractures.The orientations of these fractures are predominantly E-W,nearly N-S,NE,and NW.Specifically,the northern segment area only shows the development of regional fractures,while the southern and middle segments exhibit the development of both regional and tectonic fractures.There are three phases of tectonic fractures in different tectonic segments,and their formation times are relatively consistent.The Mesozoic tectonic events had a significant impact on the northern and central segments,with the amount of tectonic shortening and the rate of stratigraphic shortening gradually decreasing from the northeast to the southwest.The compressional stress resulting from tectonic compression also decreases from the northeast to the southwest.As a result,the development of first-phase and second-phase tectonic shear fractures is more pronounced in the northern and middle segments compared to the southern segment.Under the significant control of faults,the development of N-S-and NE-oriented fault-related fractures is more pronounced in the southern segment,while the development of NE-oriented fault-related fractures is relatively higher in the middle segment.Overall,there is an increased density of fractures and an increasing trend in fracture scale from the northern to the middle and then to the southern segment.
基金financially supported by the Hebei Provincial Natural Science Foundation of China(No.D2023402012)the Major Science and Technology Project of China National Petroleum Corporation(No.2024DJ87)。
文摘Accurately predicting relative permeability is an important issue in the research of multiphase flow in tight reservoirs.Existing predictive models typically rely on the capillary tube bundle model featuring circular cross-sections,often overlooking the impact of pore geometry on fluid flow behavior within reservoirs.In this work,the intermingled fractal theory of porous media is introduced to characterize the intricate local features within the internal space of tight rocks.Initially,iterative rules for diverse fractal units are skillfully designed to capture the actual characteristics of pore cross-sectional shapes.Subsequently,analytical relationships are derived between the iterative parameters and the area,wetted perimeter,and hydraulic diameter of pores generated by these units,followed by the establishment of a relative permeability model that considers pore geometry.The model's validity is confirmed through comparisons with experimental data and published relative permeability models,with correlation coefficients exceeding 0.996.Finally,various factors affecting two-phase flow characteristics are analyzed.The results reveal that pore geometry has a significant impact on flow behavior in porous media.Assuming that the flow channels are cylindrical typically leads to an overestimation of permeability,with the maximum relative error reaching 46.91%.Additionally,the tortuosity fractal dimension is a determinant factor influencing the relative permeability of both wetting and nonwetting fluids,and the phase permeability is sensitive to variations in solid particle size and porosity.The proposed intermingled fractal model enhances the accuracy of evaluating fluid flow characteristics in microscale pore channels and offers a novel framework for simulating porous media with complex geometries.
基金the supports provided by the CNPC Science and Technology Major Project(Grant Nos.2023ZZ05,2023ZZ0505,2023ZZ18-03)Changqing Oilfield Science and Technology Major Project(Grant No.2023DZZ01)。
文摘Tight sandstone has become an important area in gas exploration.In this study,we propose a 3D seismic reservoir parameter inversion method for tight gas-bearing sandstone reservoirs using dual neural networks.The first network referred to as the inversion network,receives seismic data and predicts reservoir parameters.At well locations,these predictions will be validated based on actual reservoir parameters to evaluate errors.For non-well locations,synthetic seismic data are generated by the application of rock physics forward modeling and seismic reflection coefficient equations.The errors are then calculated by comparing synthetic seismic data with actual seismic data.During the rock physics forward modeling,pseudo reservoir parameters are derived by perturbing the actual reservoir parameters,which are then used to generate pseudo elastic parameters through the modeling.Both the actual and pseudo parameters are then used to train the second network,referred to as the rock physics network.By incorporating the rock physics network,the method effectively alleviates issues such as gradient explosion that may arise from directly integrating rock physics computations into the network,while the inclusion of pseudo parameters enhances the network's generalization capability.The proposed method enables the direct inversion of porosity,clay conte nt,and water saturation from pre-stack seismic data using deep learning,thereby achieving quantitative predictions of reservoir rock physical parameters.The application to the field data from tight sandstone gas reservoirs in southwestern China demonstrates the method has the good capability of indicating the gas-bearing areas and provide high resolution.
