Soybean(Glycine max L.)is a globally vital crop for oil production and food security.High-quality genomic resources are instrumental for both functional genomics and breeding.Here,we report a near-complete,high-qualit...Soybean(Glycine max L.)is a globally vital crop for oil production and food security.High-quality genomic resources are instrumental for both functional genomics and breeding.Here,we report a near-complete,high-quality genome assembly of the elite cultivar Tianlong 1(TL1),featuring fully resolved telomeres and centromeres,as well as a gap-free assembly of 14 of its 20 chromosomes.On the basis of the genome assembly,we generate an ethyl methanesulfonate(EMS)-mutagenized population comprising 2555 M7 plants.Whole-genome resequencing of 288 EMS mutants uncovers 1,163,869 high-confidence single-nucleotide polymorphisms(SNPs)and 542,709 insertions/deletions(InDels),achieving 91.89%coverage of predicted protein-coding genes.Phenotypic screening demonstrates robust genotype–phenotype associations,with two nonsynonymous mutants displaying pronounced defects in seed and leaf development.Collectively,the chromosome-scale TL1 genome assembly and the extensively characterized mutant population establish valuable resources for functional genomics and precision breeding in soybean and related legume species.展开更多
‘Tianlong 1’ is widely popularized and applied with good comprehensive characters and is also good receptor for genetic transformation. However, little is known about symbiotic nitrogen fixation of ‘Tianlong 1’. I...‘Tianlong 1’ is widely popularized and applied with good comprehensive characters and is also good receptor for genetic transformation. However, little is known about symbiotic nitrogen fixation of ‘Tianlong 1’. In this report, 10 soybean rhizobia from different areas were selected to perform symbiotic matching test with ‘Tianlong 1’ in chamber. Two aboveground indicators (fresh weight aboveground and plant height) and 4 underground indicators (length of root, number of nodules, dry weight of root nodules per plant and morphology of nodules) were measured and data were statistically analyzed. Results showed that ‘Tianlong 1’ was a broad-hosted soybean which has different symbiotic matching abilities with these 10 rhizobia. Bradyrhizobium japonicum has more advantages than Sinorhizobium fredii in promoting plant growth and nodulation. 113-2 and 5036 were high effective strains. 113-2 was regarded as the most effective rhizobium matching to ‘Tianlong 1’ due to geographical advantages and adaptability. This research will promote symbiotic fixation of ‘Tianlong 1’ in agricultural industry for the first time.展开更多
基金supported by the National Natural Science Foundation of China(31970344)Joint Funds of the Natural Science Foundation of Hainan Province,China(2021JJLH0065).
文摘Soybean(Glycine max L.)is a globally vital crop for oil production and food security.High-quality genomic resources are instrumental for both functional genomics and breeding.Here,we report a near-complete,high-quality genome assembly of the elite cultivar Tianlong 1(TL1),featuring fully resolved telomeres and centromeres,as well as a gap-free assembly of 14 of its 20 chromosomes.On the basis of the genome assembly,we generate an ethyl methanesulfonate(EMS)-mutagenized population comprising 2555 M7 plants.Whole-genome resequencing of 288 EMS mutants uncovers 1,163,869 high-confidence single-nucleotide polymorphisms(SNPs)and 542,709 insertions/deletions(InDels),achieving 91.89%coverage of predicted protein-coding genes.Phenotypic screening demonstrates robust genotype–phenotype associations,with two nonsynonymous mutants displaying pronounced defects in seed and leaf development.Collectively,the chromosome-scale TL1 genome assembly and the extensively characterized mutant population establish valuable resources for functional genomics and precision breeding in soybean and related legume species.
文摘‘Tianlong 1’ is widely popularized and applied with good comprehensive characters and is also good receptor for genetic transformation. However, little is known about symbiotic nitrogen fixation of ‘Tianlong 1’. In this report, 10 soybean rhizobia from different areas were selected to perform symbiotic matching test with ‘Tianlong 1’ in chamber. Two aboveground indicators (fresh weight aboveground and plant height) and 4 underground indicators (length of root, number of nodules, dry weight of root nodules per plant and morphology of nodules) were measured and data were statistically analyzed. Results showed that ‘Tianlong 1’ was a broad-hosted soybean which has different symbiotic matching abilities with these 10 rhizobia. Bradyrhizobium japonicum has more advantages than Sinorhizobium fredii in promoting plant growth and nodulation. 113-2 and 5036 were high effective strains. 113-2 was regarded as the most effective rhizobium matching to ‘Tianlong 1’ due to geographical advantages and adaptability. This research will promote symbiotic fixation of ‘Tianlong 1’ in agricultural industry for the first time.
