Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young S...Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars,Deputy Director,Life Sciences and Medicine,USTC.展开更多
Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe R...Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe Royal Society of Chemistry(HonFRSC).展开更多
Editor-in-Chief:Peng Li Academician,Chinese Academy of Sciences Academician,The World Academy of Sciences President,Zhengzhou University Director,Tianjian Laboratory of Advanced Biomedical Sciences Professor,Tsinghua ...Editor-in-Chief:Peng Li Academician,Chinese Academy of Sciences Academician,The World Academy of Sciences President,Zhengzhou University Director,Tianjian Laboratory of Advanced Biomedical Sciences Professor,Tsinghua University Vice President,Chinese Biophysical Society President,Chinese Biophysical Society Metabolic Biology Division Advisory Board Member.展开更多
The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing W...The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof.Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2024.展开更多
Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treat...Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treatment of human hereditary disorders.Efficient base editing and expanded base conversion range have been achieved in human cells through base editors fusing with Rad51 DNA binding domain(Rad51DBD),such as hyA3A-BE4max.Here,we show that hyA3A-BE4max catalyzes C-to-T substitution in the zebrafish genome and extends editing positions(C_(12)-C_(16))proximal to the protospacer adjacent motif.We develop a codon-optimized counterpart zhyA3A-CBE5,which exhibits substantially high C-to-T conversion with 1.59-to 3.50-fold improvement compared with the original hyA3A-BE4max.With these tools,disease-relevant hereditary mutations can be more efficaciously generated in zebrafish.We introduce human genetic mutation rpl11^(Q42*)and abcc6a^(R1463C) by zhyA3A-CBE5 in zebrafish,mirroring Diamond-Blackfan anemia and Pseudoxanthoma Elasticum,respectively.Our study expands the base editing platform targeting the zebrafish genomic landscape and the application of single-base editors for disease modeling and gene function study.展开更多
Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Director of the Faculty of Clinical Nutrition,Capital Medical University.Dr.Shi received his M.D.degree at the School of Medic...Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Director of the Faculty of Clinical Nutrition,Capital Medical University.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.From 1999 to 2002,Dr.Shi was a postdoc fellow in School of Medicine,Johns Hopkins University,under the supervision of Adrian Barbul,M.D.,FACS,and then in New Jersey Medical School,Rutgers University,under the supervision of Edwin A.Deitch,M.D.,FACS.Dr.Han-Ping Shi was the Chairman of the Chinese Society for Parenteral&Enteral Nutrition(CSPEN)between 2018-2021.He is the founder Chairman of the Chinese Society of Nutritional Oncology(2012-2024),and the director of the Department of Academic,China Anti-Cancer Association(CACA)since 2017.Dr.Han-Ping Shi is currently the founder Editor-in-Chief of Electronic Journal of Metabolism and Nutrition of Cancer(Chinese),founder Editor-in-Chief of Journal of Nutritional Oncology(English)and founder Editor-in-Chief of Precision Nutrition(English).展开更多
Chuan Qin,MD,PhD,is the President of the Chinese Association for Laboratory Animal Sciences(CALAS),and the permanent professor of Chinese Academy of Medical Sciences(CAMS)&Peking Union Medical College(PUMC).Moreov...Chuan Qin,MD,PhD,is the President of the Chinese Association for Laboratory Animal Sciences(CALAS),and the permanent professor of Chinese Academy of Medical Sciences(CAMS)&Peking Union Medical College(PUMC).Moreover,she is the senior scientist of Institute of Laboratory Animal Sciences(ILAS),CAMS&PUMC.She is also the Vice-President of the Asian Federation of Laboratory Animal Science Associations(AFLAS),a board member of the International Council for Laboratory Animal Science(ICLAS),and Chairman of the National Standardization Technical Committee of Laboratory Animals.In addition to Animal Models and Experimental Medicine,Prof.Qin is also the Editor-in-Chief of Acta Laboratorium Animalis Scientia Sinica and Chinese Journal of Comparative Medicine.展开更多
Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,Ch...Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,China.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.展开更多
Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Scien...Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.展开更多
Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Scien...Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.展开更多
Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Scien...Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.展开更多
Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Scien...Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.展开更多
Yongduan Song (PhD,PE,Fellow IEEE,AAIA,CAA) received the Ph.D.degree in electrical and computer engineering from Tennessee Technological University,USA,in 1992.He held a tenured Full Professor with North Carolina A&am...Yongduan Song (PhD,PE,Fellow IEEE,AAIA,CAA) received the Ph.D.degree in electrical and computer engineering from Tennessee Technological University,USA,in 1992.He held a tenured Full Professor with North Carolina A&T State University,USA,from 1993 to 2008 and a Langley Distinguished Professor with the National Institute of Aerospace,USA,from 2005 to 2008.He is currently the Chair Professor with School of Automation,Chongqing University.展开更多
Editors-in-Chief Zhong Lin Wang,Beijing Institute of Nanoenergy and Nanosys-tems,Chinese Academy of Sciences;Nanometer Institute of the University of Chinese Academy of Sciences,China Liqun Zhang,South China Universit...Editors-in-Chief Zhong Lin Wang,Beijing Institute of Nanoenergy and Nanosys-tems,Chinese Academy of Sciences;Nanometer Institute of the University of Chinese Academy of Sciences,China Liqun Zhang,South China University of Technology,China。展开更多
文摘Quanming Lu,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars.Rongbin Zhou,Prosessor,Changjiang Distinguished Professor,National Science Fund for Distinguished Young Scholars,Deputy Director,Life Sciences and Medicine,USTC.
文摘Editor-in-Chief:Xinhe Bao Professor,President of University of Science and Technology of China,Academician of Chinese Academy of Sciences(CAS),Academician of The World Academy of Sciences(TWAS),Honorary Fellow ofthe Royal Society of Chemistry(HonFRSC).
文摘Editor-in-Chief:Peng Li Academician,Chinese Academy of Sciences Academician,The World Academy of Sciences President,Zhengzhou University Director,Tianjian Laboratory of Advanced Biomedical Sciences Professor,Tsinghua University Vice President,Chinese Biophysical Society President,Chinese Biophysical Society Metabolic Biology Division Advisory Board Member.
文摘The Editorial Office of Water Science and Engineering would like to express their sincere appreciation to the academic editors including Prof.Carlo Gualtieri from University of Napoli Federico Ⅱ,Italy,Prof.Guo-qing Wang and Prof.Zhong-zhi Fu from Nanjing Hydraulic Research Institute,China,and Prof.Yan-hui Ao,Prof.Ching-sheng Huang,Prof.Guang-qiu Jin,Prof.Bin Xu,Prof.Sai-yu Yuan,Prof.Zeng Zhou,Prof.Bo Chen,and Prof.Da-wei Guan from Hohai Uiversity,China,for their great effort and contribution to WSE in the year 2024.
基金supported by grants from Ministry of Science and Technology of the People's Republic of China(2018YFA0801004 and 2018YFA0800103)the National Natural Science Foundation of China(NSFC31530044,NSFC31970780,NSFC82202056).
文摘Single-base editors,including cytosine base editors(CBEs)and adenine base editors(ABEs),facilitate accurate C·G to T·A and A·T to G·C,respectively,holding promise for the precise modeling and treatment of human hereditary disorders.Efficient base editing and expanded base conversion range have been achieved in human cells through base editors fusing with Rad51 DNA binding domain(Rad51DBD),such as hyA3A-BE4max.Here,we show that hyA3A-BE4max catalyzes C-to-T substitution in the zebrafish genome and extends editing positions(C_(12)-C_(16))proximal to the protospacer adjacent motif.We develop a codon-optimized counterpart zhyA3A-CBE5,which exhibits substantially high C-to-T conversion with 1.59-to 3.50-fold improvement compared with the original hyA3A-BE4max.With these tools,disease-relevant hereditary mutations can be more efficaciously generated in zebrafish.We introduce human genetic mutation rpl11^(Q42*)and abcc6a^(R1463C) by zhyA3A-CBE5 in zebrafish,mirroring Diamond-Blackfan anemia and Pseudoxanthoma Elasticum,respectively.Our study expands the base editing platform targeting the zebrafish genomic landscape and the application of single-base editors for disease modeling and gene function study.
文摘Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Director of the Faculty of Clinical Nutrition,Capital Medical University.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.From 1999 to 2002,Dr.Shi was a postdoc fellow in School of Medicine,Johns Hopkins University,under the supervision of Adrian Barbul,M.D.,FACS,and then in New Jersey Medical School,Rutgers University,under the supervision of Edwin A.Deitch,M.D.,FACS.Dr.Han-Ping Shi was the Chairman of the Chinese Society for Parenteral&Enteral Nutrition(CSPEN)between 2018-2021.He is the founder Chairman of the Chinese Society of Nutritional Oncology(2012-2024),and the director of the Department of Academic,China Anti-Cancer Association(CACA)since 2017.Dr.Han-Ping Shi is currently the founder Editor-in-Chief of Electronic Journal of Metabolism and Nutrition of Cancer(Chinese),founder Editor-in-Chief of Journal of Nutritional Oncology(English)and founder Editor-in-Chief of Precision Nutrition(English).
文摘Chuan Qin,MD,PhD,is the President of the Chinese Association for Laboratory Animal Sciences(CALAS),and the permanent professor of Chinese Academy of Medical Sciences(CAMS)&Peking Union Medical College(PUMC).Moreover,she is the senior scientist of Institute of Laboratory Animal Sciences(ILAS),CAMS&PUMC.She is also the Vice-President of the Asian Federation of Laboratory Animal Science Associations(AFLAS),a board member of the International Council for Laboratory Animal Science(ICLAS),and Chairman of the National Standardization Technical Committee of Laboratory Animals.In addition to Animal Models and Experimental Medicine,Prof.Qin is also the Editor-in-Chief of Acta Laboratorium Animalis Scientia Sinica and Chinese Journal of Comparative Medicine.
文摘Han-Ping Shi,M.D.,Ph.D.,FACS,Professor of Surgery,Oncology and Nutrition.He is the current Chairman of the Department of GI Surgery/Clinical Nutrition at Beijing Shijitan Hospital,Capital Medical University,Beijing,China.Dr.Shi received his M.D.degree at the School of Medicine,Wuhan University,Wuhan,and his Ph.D.degree at the Second Military Medical University,Shanghai,China.
文摘Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.
文摘Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.
文摘Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.
文摘Editor-in-Chief Yuanming Lai,Academician of Chinese Academy of Sciences,director of Northwest Institute of Eco-Environment and Resources,Chinese Academy of Sciences,Lanzhou,China,Associate Editor of Cold Regions Science and Technology.
文摘Yongduan Song (PhD,PE,Fellow IEEE,AAIA,CAA) received the Ph.D.degree in electrical and computer engineering from Tennessee Technological University,USA,in 1992.He held a tenured Full Professor with North Carolina A&T State University,USA,from 1993 to 2008 and a Langley Distinguished Professor with the National Institute of Aerospace,USA,from 2005 to 2008.He is currently the Chair Professor with School of Automation,Chongqing University.
文摘Editors-in-Chief Zhong Lin Wang,Beijing Institute of Nanoenergy and Nanosys-tems,Chinese Academy of Sciences;Nanometer Institute of the University of Chinese Academy of Sciences,China Liqun Zhang,South China University of Technology,China。
