Under the strategic framework of rural revitalization and agricultural modernization, Xinjiashan Specialty Coffee Base, located in Zaotang Village, Lujiang Town, Longyang District, Baoshan City, has been proactively i...Under the strategic framework of rural revitalization and agricultural modernization, Xinjiashan Specialty Coffee Base, located in Zaotang Village, Lujiang Town, Longyang District, Baoshan City, has been proactively investigating innovative models for agricultural development. Through extensive communication and collaboration, this base has established close partnerships with research institutions including Kunming University of Science and Technology, Baoshan University, and Yunnan Academy of Agricultural Sciences, with a commitment to thoroughly exploring the potential for resource recycling and ecological complementarity. An innovative four-in-one three-dimensional integrated planting system incorporating "coffee, bananas, green manure, and bees" has been implemented. Concurrently, technological and digital management strategies have been comprehensively integrated to improve planting efficiency. Under this model, the proportion of specialty coffee attains 71%, and the per-unit yield is 17% greater than that of the conventional planting model. This approach not only substantially enhances economic returns but also promotes the integrated development of ecological and social benefits, offering a valuable practical example and experiential reference for the specialty and sustainable advancement of the coffee industry in comparable regions.展开更多
Aerial surveys are dynamic and continuous processes,and there are different height distributions of the ground in the measurement area,which leads to problems such as overlapping measurement areas and inaccurate altit...Aerial surveys are dynamic and continuous processes,and there are different height distributions of the ground in the measurement area,which leads to problems such as overlapping measurement areas and inaccurate altitude correction during the survey process.Commonly used terrain correction methods are based on the concept of finite elementization of ground surface radioactive sources,using GPS coordinates,radar altitude,and ground elevation distribution information from aerial surveys,combined with the sourceless efficiency calibration method to construct a response matrix,which is then inverted for surface nuclide content.However,most of the sourceless efficiency calibration methods used are numerical calculations that consider the body detector as a point detector and do not consider the changes in intrinsic detection efficiency under different incident directions of gamma rays.Therefore,when the altitude of the measurement area varies significantly or the flight altitude of the aerial survey is relatively low,such sourceless efficiency calibration method calculations tend to have a large bias,which affects the accuracy of the terrain correction.To address the above problems,this study employs a novel sourceless efficiency calibration method based on the Boolean operation of the ray deposition process and simplifies the traditional body source measurement model to a surface source measurement model to achieve fast and accurate efficiency calibration.Then,through the discretization of the measurement process,the static measurement process is superposed as equivalent to the dynamic measurement process,and the dynamic measurement response matrix is built and optimized based on the calibration method.Finally,the PSO-MLEM algorithm was used to solve the dynamic measurement response matrix to achieve dynamic terrain correction of aerial survey data.Analysis of the Baiyun'ebo test area revealed that,after applying dynamic terrain correction,the inverted anomalies in uranium(eU),thorium(eTh),and potassium(K)concentrations were closer to ground measurements(within 5.72%-30.79%)and exhibited clearer anomaly boundaries compared to traditional height-based corrections.However,owing to the inherent statistical fluctuations and characteristics of matrix inversion,higher measurement values tend to absorb lower ones,potentially enlarging the anomalous regions.Nevertheless,the highanomaly regions after inversion largely coincided with the ground truth validation,demonstrating that the proposed method can effectively correct airborne gamma spectrometry data.展开更多
According to the Mindlin plate theory and the first-order piston theory,this work obtains accurate closed-form eigensolutions for the flutter problem of three-dimensional(3D)rectangular laminated panels.The governing ...According to the Mindlin plate theory and the first-order piston theory,this work obtains accurate closed-form eigensolutions for the flutter problem of three-dimensional(3D)rectangular laminated panels.The governing differential equations are derived by the Hamilton's variational principle,and then solved by the iterative Separation-of-Variable(i SOV)method,which are applicable to arbitrary combinations of homogeneous Boundary Conditions(BCs).However,only the simply-support,clamped and cantilever panels are considered in this work for the sake of clarity.With the closed-form eigensolutions,the flutter frequency,flutter mode and flutter boundary are presented,and the effect of shear deformation and aerodynamic damping on flutter frequencies is investigated.Besides,the relation between panel energy and the work of aerodynamic load is discussed.The numerical comparisons reveal the following.(A)The flutter eigenvalues obtained by the present method are accurate,validated by the Finite Element Method(FEM)and the Galerkin method.(B)When the span-chord ratio is larger than 3,simplifying a 3D panel to 2D(two-dimensional)panel is reasonable and the relative differences of the flutter points predicted by the two models are less than one percent.(C)The reciprocal relationship between the mechanical energy of the panel and the work done by aerodynamic load is verified by using the present flutter eigenvalues and modes,further indicating the high accuracy of the present solutions.(D)The coupling of shear deformation and aerodynamic damping prevents frequency coalescing.展开更多
In the rapidly evolving landscape of digital transformation and industrial integration,higher education faces the challenge of cultivating applied talents equipped with interdisciplinary knowledge,engineering skills,a...In the rapidly evolving landscape of digital transformation and industrial integration,higher education faces the challenge of cultivating applied talents equipped with interdisciplinary knowledge,engineering skills,and innovative thinking.Traditional teaching models often fail to bridge the gap between theoretical knowledge and practical application,resulting in passive learning and limited problem-solving capabilities.This paper proposes a three-dimensional integrated teaching model centered on“Information Technology-Domain Knowledge-Outcome Production”(the“2+2+2”credit framework)to address these challenges.Drawing on constructivist theories,Bloom’s Taxonomy,and the CDIO model,the framework uses real projects to drive learning,facilitating the seamless integration of theoretical teaching and practical innovation.The model emphasizes tiered teaching objectives and interdisciplinary pathways,supported by dynamic assessment systems that track students’growth in knowledge,skills,and abilities.Applied in smart health and financial technology domains,this approach enhances students’comprehensive capabilities,aligning educational outcomes with industry demands.This study offers replicable strategies for educational reform in new engineering disciplines,aiming to transform students into proactive innovators and versatile talents.展开更多
Fracture surface contour study is one of the important requirements for characterization and evaluation of the microstructure of rocks.Based on the improved cube covering method and the 3D contour digital reconstructi...Fracture surface contour study is one of the important requirements for characterization and evaluation of the microstructure of rocks.Based on the improved cube covering method and the 3D contour digital reconstruction model,this study proposes a quantitative microstructure characterization method combining the roughness evaluation index and the 3D fractal dimension to study the change rule of the fracture surface morphology after blasting.This method was applied and validated in the study of the fracture microstructure of the rock after blasting.The results show that the fracture morphology characteristics of the 3D contour digital reconstruction model have good correlation with the changes of the blasting action.The undulation rate of the three-dimensional surface profile of the rock is more prone to dramatic rise and dramatic fall morphology.In terms of tilting trend,the tilting direction also shows gradual disorder,with the tilting angle increasing correspondingly.All the roughness evaluation indexes of the rock fissure surface after blasting show a linear and gradually increasing trend as the distance to the bursting center increases;the difference between the two-dimensional roughness evaluation indexes and the three-dimensional ones of the same micro-area rock samples also becomes increasingly larger,among which the three-dimensional fissure roughness coefficient JRC and the surface roughness ratio Rs display better correlation.Compared with the linear fitting formula of the power function relationship,the three-dimensional fractal dimension of the postblast fissure surface is fitted with the values of JRC and Rs,which renders higher correlation coefficients,and the degree of linear fitting of JRC to the three-dimensional fractal dimension is higher.The fractal characteristics of the blast-affected region form a unity with the three-dimensional roughness evaluation of the fissure surface.展开更多
It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimens...It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass.展开更多
To improve the human-physical-virtual coordination and integration of the digital twin workshop,3D visual monitoring and human-computer interaction of the digital twin workshop was studied.First,a novel 6D model of th...To improve the human-physical-virtual coordination and integration of the digital twin workshop,3D visual monitoring and human-computer interaction of the digital twin workshop was studied.First,a novel 6D model of the 3D visualization interactive system for digital twin workshops is proposed.As the traditional 5D digital twin model ignores the importance of human-computer interaction,a new dimension of the user terminal was added.A hierarchical real-time data-driven mapping model for the workshop production process is then proposed.Moreover,a real-time data acquisition method for the industrial Internet of things is proposed based on OPC UA(object linking and embedding for process control unified architecture).Based on the 6D model of the system,the process of creating a 3D visualization virtual environment based on virtual reality is introduced,in addition to a data-driven process based on the data management cloud platform.Finally,the 6D model of the system was confirmed using the blade rotor test workshop as the object,and a 3D visualization interactive system is developed.The results show that the system is more transparent,real-time,data-driven and more efficient,as well as promotes the coordination and integration of human-physical-virtual,which has practical significance for developing digital twin workshops.展开更多
The complete stress-strain characteristics of sandstone specimens were investigated in a series of quasistatic monotonic uniaxial compression tests.Strain patterns development during pre-and post-peak behaviours in sp...The complete stress-strain characteristics of sandstone specimens were investigated in a series of quasistatic monotonic uniaxial compression tests.Strain patterns development during pre-and post-peak behaviours in specimens with different aspect ratios was also examined.Peak stress,post-peak portion of stress-strain,brittleness,characteristics of progressive localisation and field strain patterns development were affected at different extents by specimen aspect ratio.Strain patterns of the rocks were obtained by applying three-dimensional(3D) digital image correlation(DIC) technique.Unlike conventional strain measurement using strain gauges attached to specimen,3D DIC allowed not only measuring large strains,but more importantly,mapping the development of field strain throughout the compression test,i.e.in pre-and post-peak regimes.Field strain development in the surface of rock specimen suggests that strain starts localising progressively and develops at a lower rate in pre-peak regime.However,in post-peak regime,strains increase at different rates as local deformations take place at different extents in the vicinity and outside the localised zone.The extent of localised strains together with the rate of strain localisation is associated with the increase in rate of strength degradation.Strain localisation and local inelastic unloading outside the localised zone both feature post-peak regime.展开更多
We present an optical encryption method of multiple three-dimensional objects based on multiple interferences and single-pixel digital holography. By modifying the Mach-Zehnder interferometer, the interference of the ...We present an optical encryption method of multiple three-dimensional objects based on multiple interferences and single-pixel digital holography. By modifying the Mach-Zehnder interferometer, the interference of the multiple objects beams and the one reference beam is used to simultaneously encrypt multiple objects into a ciphertext. During decryption, each three-dimensional object can be decrypted independently without having to decrypt other objects. Since the single- pixel digital holography based on compressive sensing theory is introduced, the encrypted data of this method is effectively reduced. In addition, recording fewer encrypted data can greatly reduce the bandwidth of network transmission. Moreover, the compressive sensing essentially serves as a secret key that makes an intruder attack invalid, which means that the system is more secure than the conventional encryption method. Simulation results demonstrate the feasibility of the proposed method and show that the system has good security performance.展开更多
To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D lea...To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.展开更多
Objective:To evaluate the clinical efficacy of the preoperative digita1 design combined with three dimensional(3D)printing models to assist percutaneous kyphoplasty(PKP)treatment for thoracolumbar compression frac tur...Objective:To evaluate the clinical efficacy of the preoperative digita1 design combined with three dimensional(3D)printing models to assist percutaneous kyphoplasty(PKP)treatment for thoracolumbar compression frac tures.Methods:From January 2018 to August 2020,we obtained data of 99 patients diagnosed thoracolumbar compression fractures.These patients were divided into control group(n=50)underwent traditional PKP surgery,and observation group(n=49)underwent preoperative digital design combined with 3D printing model assisted PKP treatment.The clinical efficacy was evaluated with five parameters,including operation time,number of intraoperative radiographs,visual analogue scale(VAS)score,Cobb Angle change,and high compression rate of injured vertebrae.Results:There were statistically significant differences of operation time and number of intraoperative radio graphs between the two groups(P<0.05).For VAS score,Cobb Angle change and vertebral height compression rate,all of these three parameters were significantly improved when the patients accepted surgery teatment in two groups(P<0.05).However,there were no significant differences between control group and observation group for these three parameters either before or after surgery(P>0.05).Conclusions:Through the design of preoperative surgical guide plate and the application of 3D printing model to guide the operation,the precise design of preoperative surgical puncture site and puncture Angle of the injured vertebra was realized,the number of intraoperative radiographs was reduced,the operation time was shortened and the operation efficiency was improved.展开更多
Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significan...Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significant gap in detailed and consistent data on 3D building space structures with global coverage due to the challenges inherent in the data collection and model calibration processes.In this study,we constructed a global urban structure(GUS-3D)dataset,including building volume,height,and footprint information,at a 500 m spatial resolution using extensive satellite observation products and numerous reference building samples.Our analysis indicated that the total volume of buildings worldwide in2015 exceeded 1×10^(12)m^(3).Over the 1985 to 2015 period,we observed a slight increase in the magnitude of 3D building volume growth(i.e.,it increased from 166.02 km3 during the 1985–2000 period to 175.08km3 during the 2000–2015 period),while the expansion magnitudes of the two-dimensional(2D)building footprint(22.51×10^(3) vs 13.29×10^(3)km^(2))and urban extent(157×10^(3) vs 133.8×10^(3)km^(2))notably decreased.This trend highlights the significant increase in intensive vertical utilization of urban land.Furthermore,we identified significant heterogeneity in building space provision and inequality across cities worldwide.This inequality is particularly pronounced in many populous Asian cities,which has been overlooked in previous studies on economic inequality.The GUS-3D dataset shows great potential to deepen our understanding of the urban environment and creates new horizons for numerous 3D urban studies.展开更多
BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are ne...BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are necessary.However,due to differences in race and sex,people’s body shapes vary.According to European guidelines,the patch should measure 10 cm×15 cm.If any part of the MPO is dissected,injury to the nerves,vascular network,or organs may occur during surgery,thereby leading to inguinal discomfort,pain,and seroma formation after surgery.Therefore,accurate localization and measurement of the boundary of the MPO are crucial for selecting the optimal patch for inguinal hernia repair.AIM To compare the size of the MPO measured on three-dimensional multislice spiral computed tomography(CT)with that measured via laparoscopy and explore the relevant factors influencing the size of the MPO.METHODS Clinical data from 74 patients who underwent laparoscopic tension-free inguinal hernia repair at the General Surgery Department of the First Affiliated Hospital of Anhui University of Science and Technology between September 2022 and July 2024 were collected and analyzed retrospectively.Transabdominal preperitoneal was performed.Sixty-four males and 10 females,with an average age of 58.30±12.32 years,were included.The clinical data of the patients were collected.The boundary of the MPO was measured on three-dimensional CT images before surgery and then again during transabdominal preperitoneal.All the preoperative and intraoperative data were analyzed via paired t-tests.A t-test was used for comparisons of age,body mass index,and sex between the groups.In the comparative analysis,a P value less than 0.05 indicated a significant difference.RESULTS The boundaries of the MPO on 3-dimensional CT images measured 7.05±0.47 cm and 6.27±0.61 cm,and the area of the MPO was 19.54±3.33 cm^(2).The boundaries of the MPO during surgery were 7.18±0.51 cm and 6.17±0.40 cm.The errors were not statistically significant.However,the intraoperative BD(the width of the MPO,P=0.024,P<0.05)and preoperative AC(the length of the MPO,P=0.045,P<0.05)significantly differed according to sex.The AC and BD measurements before and during surgery were not significantly different according to age,body mass index,hernia side or hernia type(P>0.05).CONCLUSION The application of this technology can aid in determining the most appropriate dissection range and patch size.展开更多
Digitization is the inevitable path for the natural development of traditional Chinese medicine(TCM)in the context of the Fourth Industrial Revolution.The goal of TCM digitization is to generate intelligence from numb...Digitization is the inevitable path for the natural development of traditional Chinese medicine(TCM)in the context of the Fourth Industrial Revolution.The goal of TCM digitization is to generate intelligence from numbers.Originating from the reasoning paradigm of Xiangshu(象数,image-number)or phenotype-numerology thinking,TCM came with a deep correlation of clinical observations with digits and laid a strong theoretical basis for digitization.The digitization of TCM should start from the clinical aspect,solve the problem of electronic medical records,achieve standardization and informatization,and on this basis,form a TCM knowledge base through knowledge-building.This process depends on the combined efforts of multiple disciplines such as medicine,mathematics,and engineering to achieve the digitization and intelligent transformation of TCM.This era calls for TCM to break down barriers,embrace opportunities,and move towards digitization.However,during the transformation,it should maintain its essence,avoid simplistic conversions,be guided by scientific value,leverage cutting-edge technologies,and enhance the depth and breadth of the interpretation of TCM connotations.The digitization of TCM will also improve its service capabilities,create an innovative digitally-intelligent TCM service platform,and contribute to the development of“Healthy China”initiatives with wisdom and solutions.展开更多
The development of efficient catalysts for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)is of great significance for the practical application of water splitting in alkaline electrolytes.Transitio...The development of efficient catalysts for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)is of great significance for the practical application of water splitting in alkaline electrolytes.Transition metal sulfide electrocatalysts have been widely recognized as efficient catalysts for water splitting in alkaline media.In this work,an original and efficient synthesis strategy is proposed for the fabrication of asymmetric anode(N-(Co-Cu)S_(x))and cathode(N-CoS/Cu_(2)S).Impressively,these electrodes exhibit superior performance,benefiting from the construction of three-dimensional(3D)structures and the electronic structure adjustment caused by N-doping with increased active sites,improved mass/charge transport and enhanced evolution and release of gas bubbles.Hence,N-(Co-Cu)S_(x)anode exhibits excellent OER performance with only 217 mV overpotential at 10 mA·cm^(-2),while N-CoS/Cu_(2)S cathode possesses excellent HER performance with only 67 mV overpotential at 10 mA·cm^(-2).N-(Co-Cu)S_(x)||N-CoS/Cu_(2)S electrolyzer presents a low cell voltage of 1.53 V at 10 mA·cm^(-2)toward overall water splitting,which is superior to most recently reported transition metal sulfide-based catalysts.展开更多
The problem of taking an unorganized point cloud in 3D space and fitting a polyhedral surface to those points is both important and difficult. Aiming at increasing applications of full three dimensional digital terrai...The problem of taking an unorganized point cloud in 3D space and fitting a polyhedral surface to those points is both important and difficult. Aiming at increasing applications of full three dimensional digital terrain surface modeling, a new algorithm for the automatic generation of three dimensional triangulated irregular network from a point cloud is pro- posed. Based on the local topological consistency test, a combined algorithm of constrained 3D Delaunay triangulation and region-growing is extended to ensure topologically correct reconstruction. This paper also introduced an efficient neighbor- ing triangle location method by making full use of the surface normal information. Experimental results prove that this algo- rithm can efficiently obtain the most reasonable reconstructed mesh surface with arbitrary topology, wherein the automati- cally reconstructed surface has only small topological difference from the true surface. This algorithm has potential applica- tions to virtual environments, computer vision, and so on.展开更多
Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade compone...Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade components.In this paper,a dynamic model of 3D 4-directional braided composite thin plates considering braiding directions is established.Based on Kirchhoff's plate assumptions,the displacement variables of the plate are expressed.By incorporating the braiding directions into the constitutive equation of the braided composites,the dynamic model of the plate considering braiding directions is obtained.The effects of the speeds,braiding directions,and braided angles on the responses of the plate with fixed-axis rotation and translational motion,respectively,are investigated.This paper presents a dynamic theory for calculating the deformation of 3D braided composite structures undergoing both translational and rotational motions.It also provides a simulation method for investigating the dynamic behavior of non-isotropic material plates in various applications.展开更多
This paper proposes an attitude control strategy for a flexible satellite equipped with an orthogonal cluster of three-dimensional(3D)magnetically suspended wheels(MSWs).The mathematical model for the satellite incorp...This paper proposes an attitude control strategy for a flexible satellite equipped with an orthogonal cluster of three-dimensional(3D)magnetically suspended wheels(MSWs).The mathematical model for the satellite incorporating flexible appendages and an orthogonal cluster of magnetically suspended reaction wheel actuators is initially developed.After that,an adaptive attitude controller is designed with a switching surface of variable structure,an adaptive law for estimating inertia matrix uncertainty,and a fuzzy disturbance observer for estimating disturbance torques.Additionally,a Moore-Penrose-based steering law is proposed to derive the tilt angle commands of the orthogonal configuration of the 3D MSW to follow the designed control signal.Finally,numerical simulations are presented to validate the effectiveness of the proposed control strategy.展开更多
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ...Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.展开更多
文摘Under the strategic framework of rural revitalization and agricultural modernization, Xinjiashan Specialty Coffee Base, located in Zaotang Village, Lujiang Town, Longyang District, Baoshan City, has been proactively investigating innovative models for agricultural development. Through extensive communication and collaboration, this base has established close partnerships with research institutions including Kunming University of Science and Technology, Baoshan University, and Yunnan Academy of Agricultural Sciences, with a commitment to thoroughly exploring the potential for resource recycling and ecological complementarity. An innovative four-in-one three-dimensional integrated planting system incorporating "coffee, bananas, green manure, and bees" has been implemented. Concurrently, technological and digital management strategies have been comprehensively integrated to improve planting efficiency. Under this model, the proportion of specialty coffee attains 71%, and the per-unit yield is 17% greater than that of the conventional planting model. This approach not only substantially enhances economic returns but also promotes the integrated development of ecological and social benefits, offering a valuable practical example and experiential reference for the specialty and sustainable advancement of the coffee industry in comparable regions.
基金supported by the National Key Research and Development Program(No.2022YFC2807400)the National Natural Science Foundation of China(Nos.12265003 and 12205044)。
文摘Aerial surveys are dynamic and continuous processes,and there are different height distributions of the ground in the measurement area,which leads to problems such as overlapping measurement areas and inaccurate altitude correction during the survey process.Commonly used terrain correction methods are based on the concept of finite elementization of ground surface radioactive sources,using GPS coordinates,radar altitude,and ground elevation distribution information from aerial surveys,combined with the sourceless efficiency calibration method to construct a response matrix,which is then inverted for surface nuclide content.However,most of the sourceless efficiency calibration methods used are numerical calculations that consider the body detector as a point detector and do not consider the changes in intrinsic detection efficiency under different incident directions of gamma rays.Therefore,when the altitude of the measurement area varies significantly or the flight altitude of the aerial survey is relatively low,such sourceless efficiency calibration method calculations tend to have a large bias,which affects the accuracy of the terrain correction.To address the above problems,this study employs a novel sourceless efficiency calibration method based on the Boolean operation of the ray deposition process and simplifies the traditional body source measurement model to a surface source measurement model to achieve fast and accurate efficiency calibration.Then,through the discretization of the measurement process,the static measurement process is superposed as equivalent to the dynamic measurement process,and the dynamic measurement response matrix is built and optimized based on the calibration method.Finally,the PSO-MLEM algorithm was used to solve the dynamic measurement response matrix to achieve dynamic terrain correction of aerial survey data.Analysis of the Baiyun'ebo test area revealed that,after applying dynamic terrain correction,the inverted anomalies in uranium(eU),thorium(eTh),and potassium(K)concentrations were closer to ground measurements(within 5.72%-30.79%)and exhibited clearer anomaly boundaries compared to traditional height-based corrections.However,owing to the inherent statistical fluctuations and characteristics of matrix inversion,higher measurement values tend to absorb lower ones,potentially enlarging the anomalous regions.Nevertheless,the highanomaly regions after inversion largely coincided with the ground truth validation,demonstrating that the proposed method can effectively correct airborne gamma spectrometry data.
基金support of the National Natural Science Foundation of China(No.12172023)。
文摘According to the Mindlin plate theory and the first-order piston theory,this work obtains accurate closed-form eigensolutions for the flutter problem of three-dimensional(3D)rectangular laminated panels.The governing differential equations are derived by the Hamilton's variational principle,and then solved by the iterative Separation-of-Variable(i SOV)method,which are applicable to arbitrary combinations of homogeneous Boundary Conditions(BCs).However,only the simply-support,clamped and cantilever panels are considered in this work for the sake of clarity.With the closed-form eigensolutions,the flutter frequency,flutter mode and flutter boundary are presented,and the effect of shear deformation and aerodynamic damping on flutter frequencies is investigated.Besides,the relation between panel energy and the work of aerodynamic load is discussed.The numerical comparisons reveal the following.(A)The flutter eigenvalues obtained by the present method are accurate,validated by the Finite Element Method(FEM)and the Galerkin method.(B)When the span-chord ratio is larger than 3,simplifying a 3D panel to 2D(two-dimensional)panel is reasonable and the relative differences of the flutter points predicted by the two models are less than one percent.(C)The reciprocal relationship between the mechanical energy of the panel and the work done by aerodynamic load is verified by using the present flutter eigenvalues and modes,further indicating the high accuracy of the present solutions.(D)The coupling of shear deformation and aerodynamic damping prevents frequency coalescing.
文摘In the rapidly evolving landscape of digital transformation and industrial integration,higher education faces the challenge of cultivating applied talents equipped with interdisciplinary knowledge,engineering skills,and innovative thinking.Traditional teaching models often fail to bridge the gap between theoretical knowledge and practical application,resulting in passive learning and limited problem-solving capabilities.This paper proposes a three-dimensional integrated teaching model centered on“Information Technology-Domain Knowledge-Outcome Production”(the“2+2+2”credit framework)to address these challenges.Drawing on constructivist theories,Bloom’s Taxonomy,and the CDIO model,the framework uses real projects to drive learning,facilitating the seamless integration of theoretical teaching and practical innovation.The model emphasizes tiered teaching objectives and interdisciplinary pathways,supported by dynamic assessment systems that track students’growth in knowledge,skills,and abilities.Applied in smart health and financial technology domains,this approach enhances students’comprehensive capabilities,aligning educational outcomes with industry demands.This study offers replicable strategies for educational reform in new engineering disciplines,aiming to transform students into proactive innovators and versatile talents.
基金National Key Research and Development Program of China,Grant/Award Number:2021YFC2902103National Natural Science Foundation of China,Grant/Award Number:51934001Fundamental Research Funds for the Central Universities,Grant/Award Number:2023JCCXLJ02。
文摘Fracture surface contour study is one of the important requirements for characterization and evaluation of the microstructure of rocks.Based on the improved cube covering method and the 3D contour digital reconstruction model,this study proposes a quantitative microstructure characterization method combining the roughness evaluation index and the 3D fractal dimension to study the change rule of the fracture surface morphology after blasting.This method was applied and validated in the study of the fracture microstructure of the rock after blasting.The results show that the fracture morphology characteristics of the 3D contour digital reconstruction model have good correlation with the changes of the blasting action.The undulation rate of the three-dimensional surface profile of the rock is more prone to dramatic rise and dramatic fall morphology.In terms of tilting trend,the tilting direction also shows gradual disorder,with the tilting angle increasing correspondingly.All the roughness evaluation indexes of the rock fissure surface after blasting show a linear and gradually increasing trend as the distance to the bursting center increases;the difference between the two-dimensional roughness evaluation indexes and the three-dimensional ones of the same micro-area rock samples also becomes increasingly larger,among which the three-dimensional fissure roughness coefficient JRC and the surface roughness ratio Rs display better correlation.Compared with the linear fitting formula of the power function relationship,the three-dimensional fractal dimension of the postblast fissure surface is fitted with the values of JRC and Rs,which renders higher correlation coefficients,and the degree of linear fitting of JRC to the three-dimensional fractal dimension is higher.The fractal characteristics of the blast-affected region form a unity with the three-dimensional roughness evaluation of the fissure surface.
基金supported by grants from the Human Resources Development program (Grant No.20204010600250)the Training Program of CCUS for the Green Growth (Grant No.20214000000500)by the Korea Institute of Energy Technology Evaluation and Planning (KETEP)funded by the Ministry of Trade,Industry,and Energy of the Korean Government (MOTIE).
文摘It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass.
基金The National Natural Science Foundation of China(No.51875332)the Capacity Building Projects of Some Local Universities of Shanghai Science and Technology Commission(No.18040501600).
文摘To improve the human-physical-virtual coordination and integration of the digital twin workshop,3D visual monitoring and human-computer interaction of the digital twin workshop was studied.First,a novel 6D model of the 3D visualization interactive system for digital twin workshops is proposed.As the traditional 5D digital twin model ignores the importance of human-computer interaction,a new dimension of the user terminal was added.A hierarchical real-time data-driven mapping model for the workshop production process is then proposed.Moreover,a real-time data acquisition method for the industrial Internet of things is proposed based on OPC UA(object linking and embedding for process control unified architecture).Based on the 6D model of the system,the process of creating a 3D visualization virtual environment based on virtual reality is introduced,in addition to a data-driven process based on the data management cloud platform.Finally,the 6D model of the system was confirmed using the blade rotor test workshop as the object,and a 3D visualization interactive system is developed.The results show that the system is more transparent,real-time,data-driven and more efficient,as well as promotes the coordination and integration of human-physical-virtual,which has practical significance for developing digital twin workshops.
基金supported by the Deep Exploration Technologies Cooperative Research Centre whose activities are funded by the Australian Government's Cooperative Research Centre Programme.This is DET CRC Document 2017/954
文摘The complete stress-strain characteristics of sandstone specimens were investigated in a series of quasistatic monotonic uniaxial compression tests.Strain patterns development during pre-and post-peak behaviours in specimens with different aspect ratios was also examined.Peak stress,post-peak portion of stress-strain,brittleness,characteristics of progressive localisation and field strain patterns development were affected at different extents by specimen aspect ratio.Strain patterns of the rocks were obtained by applying three-dimensional(3D) digital image correlation(DIC) technique.Unlike conventional strain measurement using strain gauges attached to specimen,3D DIC allowed not only measuring large strains,but more importantly,mapping the development of field strain throughout the compression test,i.e.in pre-and post-peak regimes.Field strain development in the surface of rock specimen suggests that strain starts localising progressively and develops at a lower rate in pre-peak regime.However,in post-peak regime,strains increase at different rates as local deformations take place at different extents in the vicinity and outside the localised zone.The extent of localised strains together with the rate of strain localisation is associated with the increase in rate of strength degradation.Strain localisation and local inelastic unloading outside the localised zone both feature post-peak regime.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61405130 and 61320106015)
文摘We present an optical encryption method of multiple three-dimensional objects based on multiple interferences and single-pixel digital holography. By modifying the Mach-Zehnder interferometer, the interference of the multiple objects beams and the one reference beam is used to simultaneously encrypt multiple objects into a ciphertext. During decryption, each three-dimensional object can be decrypted independently without having to decrypt other objects. Since the single- pixel digital holography based on compressive sensing theory is introduced, the encrypted data of this method is effectively reduced. In addition, recording fewer encrypted data can greatly reduce the bandwidth of network transmission. Moreover, the compressive sensing essentially serves as a secret key that makes an intruder attack invalid, which means that the system is more secure than the conventional encryption method. Simulation results demonstrate the feasibility of the proposed method and show that the system has good security performance.
文摘To address the problem of multi-missile cooperative interception against maneuvering targets at a prespecified impact time and desired Line-of-Sight(LOS)angles in ThreeDimensional(3D)space,this paper proposes a 3D leader-following cooperative interception guidance law.First,in the LOS direction of the leader,an impact time-controlled guidance law is derived based on the fixed-time stability theory,which enables the leader to complete the interception task at a prespecified impact time.Next,in the LOS direction of the followers,by introducing a time consensus tracking error function,a fixed-time consensus tracking guidance law is investigated to guarantee the consensus tracking convergence of the time-to-go.Then,in the direction normal to the LOS,by combining the designed global integral sliding mode surface and the second-order Sliding Mode Control(SMC)theory,an innovative 3D LOS-angle-constrained interception guidance law is developed,which eliminates the reaching phase in the traditional sliding mode guidance laws and effectively saves energy consumption.Moreover,it effectively suppresses the chattering phenomenon while avoiding the singularity issue,and compensates for unknown interference caused by target maneuvering online,making it convenient for practical engineering applications.Finally,theoretical proof analysis and multiple sets of numerical simulation results verify the effectiveness,superiority,and robustness of the investigated guidance law.
基金supported in part by the General Program of Natural Science Foundation of Hubei Province,China(Grant No.2020CFB548)a Project in 2021 of Science and Technology Support Plan of Guizhou Province,China(Grant No.202158413293820389).
文摘Objective:To evaluate the clinical efficacy of the preoperative digita1 design combined with three dimensional(3D)printing models to assist percutaneous kyphoplasty(PKP)treatment for thoracolumbar compression frac tures.Methods:From January 2018 to August 2020,we obtained data of 99 patients diagnosed thoracolumbar compression fractures.These patients were divided into control group(n=50)underwent traditional PKP surgery,and observation group(n=49)underwent preoperative digital design combined with 3D printing model assisted PKP treatment.The clinical efficacy was evaluated with five parameters,including operation time,number of intraoperative radiographs,visual analogue scale(VAS)score,Cobb Angle change,and high compression rate of injured vertebrae.Results:There were statistically significant differences of operation time and number of intraoperative radio graphs between the two groups(P<0.05).For VAS score,Cobb Angle change and vertebral height compression rate,all of these three parameters were significantly improved when the patients accepted surgery teatment in two groups(P<0.05).However,there were no significant differences between control group and observation group for these three parameters either before or after surgery(P>0.05).Conclusions:Through the design of preoperative surgical guide plate and the application of 3D printing model to guide the operation,the precise design of preoperative surgical puncture site and puncture Angle of the injured vertebra was realized,the number of intraoperative radiographs was reduced,the operation time was shortened and the operation efficiency was improved.
基金supported by the National Science Fund for Distinguished Young Scholars(42225107)the National Natural Science Foundation of China(42001326,42371414,42171409,and 42271419)+1 种基金the Natural Science Foundation of Guangdong Province of China(2022A1515012207)the Basic and Applied Basic Research Project of Guangzhou Science and Technology Planning(202201011539)。
文摘Three-dimensional(3D)urban structures play a critical role in informing climate mitigation strategies aimed at the built environment and facilitating sustainable urban development.Regrettably,there exists a significant gap in detailed and consistent data on 3D building space structures with global coverage due to the challenges inherent in the data collection and model calibration processes.In this study,we constructed a global urban structure(GUS-3D)dataset,including building volume,height,and footprint information,at a 500 m spatial resolution using extensive satellite observation products and numerous reference building samples.Our analysis indicated that the total volume of buildings worldwide in2015 exceeded 1×10^(12)m^(3).Over the 1985 to 2015 period,we observed a slight increase in the magnitude of 3D building volume growth(i.e.,it increased from 166.02 km3 during the 1985–2000 period to 175.08km3 during the 2000–2015 period),while the expansion magnitudes of the two-dimensional(2D)building footprint(22.51×10^(3) vs 13.29×10^(3)km^(2))and urban extent(157×10^(3) vs 133.8×10^(3)km^(2))notably decreased.This trend highlights the significant increase in intensive vertical utilization of urban land.Furthermore,we identified significant heterogeneity in building space provision and inequality across cities worldwide.This inequality is particularly pronounced in many populous Asian cities,which has been overlooked in previous studies on economic inequality.The GUS-3D dataset shows great potential to deepen our understanding of the urban environment and creates new horizons for numerous 3D urban studies.
基金Supported by the 2022 Provincial Quality Engineering Project for Higher Education Institutions,No.2022sx031the 2023 Provincial Quality Engineering Project for Higher Education Institutions,No.2023jyxm1071.
文摘BACKGROUND Inguinal hernias are common after surgery.Tension-free repair is widely accepted as the main method for managing inguinal hernias.Adequate exposure,coverage,and repair of the myopectineal orifice(MPO)are necessary.However,due to differences in race and sex,people’s body shapes vary.According to European guidelines,the patch should measure 10 cm×15 cm.If any part of the MPO is dissected,injury to the nerves,vascular network,or organs may occur during surgery,thereby leading to inguinal discomfort,pain,and seroma formation after surgery.Therefore,accurate localization and measurement of the boundary of the MPO are crucial for selecting the optimal patch for inguinal hernia repair.AIM To compare the size of the MPO measured on three-dimensional multislice spiral computed tomography(CT)with that measured via laparoscopy and explore the relevant factors influencing the size of the MPO.METHODS Clinical data from 74 patients who underwent laparoscopic tension-free inguinal hernia repair at the General Surgery Department of the First Affiliated Hospital of Anhui University of Science and Technology between September 2022 and July 2024 were collected and analyzed retrospectively.Transabdominal preperitoneal was performed.Sixty-four males and 10 females,with an average age of 58.30±12.32 years,were included.The clinical data of the patients were collected.The boundary of the MPO was measured on three-dimensional CT images before surgery and then again during transabdominal preperitoneal.All the preoperative and intraoperative data were analyzed via paired t-tests.A t-test was used for comparisons of age,body mass index,and sex between the groups.In the comparative analysis,a P value less than 0.05 indicated a significant difference.RESULTS The boundaries of the MPO on 3-dimensional CT images measured 7.05±0.47 cm and 6.27±0.61 cm,and the area of the MPO was 19.54±3.33 cm^(2).The boundaries of the MPO during surgery were 7.18±0.51 cm and 6.17±0.40 cm.The errors were not statistically significant.However,the intraoperative BD(the width of the MPO,P=0.024,P<0.05)and preoperative AC(the length of the MPO,P=0.045,P<0.05)significantly differed according to sex.The AC and BD measurements before and during surgery were not significantly different according to age,body mass index,hernia side or hernia type(P>0.05).CONCLUSION The application of this technology can aid in determining the most appropriate dissection range and patch size.
基金Special Funds of the National Natural Science Foundation of China(T2341006).
文摘Digitization is the inevitable path for the natural development of traditional Chinese medicine(TCM)in the context of the Fourth Industrial Revolution.The goal of TCM digitization is to generate intelligence from numbers.Originating from the reasoning paradigm of Xiangshu(象数,image-number)or phenotype-numerology thinking,TCM came with a deep correlation of clinical observations with digits and laid a strong theoretical basis for digitization.The digitization of TCM should start from the clinical aspect,solve the problem of electronic medical records,achieve standardization and informatization,and on this basis,form a TCM knowledge base through knowledge-building.This process depends on the combined efforts of multiple disciplines such as medicine,mathematics,and engineering to achieve the digitization and intelligent transformation of TCM.This era calls for TCM to break down barriers,embrace opportunities,and move towards digitization.However,during the transformation,it should maintain its essence,avoid simplistic conversions,be guided by scientific value,leverage cutting-edge technologies,and enhance the depth and breadth of the interpretation of TCM connotations.The digitization of TCM will also improve its service capabilities,create an innovative digitally-intelligent TCM service platform,and contribute to the development of“Healthy China”initiatives with wisdom and solutions.
基金supported by the Science and Technology Project of Southwest Petroleum University(No.2021JBGS03)the Local Science and Technology Development Fund Projects Guided by the Central Government of China(No.2021ZYD0060)+2 种基金the National Natural Science Foundation of China(Nos.22209143 and 52371241)Guangdong High-level Innovation Institute Project(Nos.2021B0909050001 and 2021CX02L365)Guangdong Basic and Applied Basic Research Foundation(No.2023B1515120095).
文摘The development of efficient catalysts for hydrogen evolution reaction(HER)and oxygen evolution reaction(OER)is of great significance for the practical application of water splitting in alkaline electrolytes.Transition metal sulfide electrocatalysts have been widely recognized as efficient catalysts for water splitting in alkaline media.In this work,an original and efficient synthesis strategy is proposed for the fabrication of asymmetric anode(N-(Co-Cu)S_(x))and cathode(N-CoS/Cu_(2)S).Impressively,these electrodes exhibit superior performance,benefiting from the construction of three-dimensional(3D)structures and the electronic structure adjustment caused by N-doping with increased active sites,improved mass/charge transport and enhanced evolution and release of gas bubbles.Hence,N-(Co-Cu)S_(x)anode exhibits excellent OER performance with only 217 mV overpotential at 10 mA·cm^(-2),while N-CoS/Cu_(2)S cathode possesses excellent HER performance with only 67 mV overpotential at 10 mA·cm^(-2).N-(Co-Cu)S_(x)||N-CoS/Cu_(2)S electrolyzer presents a low cell voltage of 1.53 V at 10 mA·cm^(-2)toward overall water splitting,which is superior to most recently reported transition metal sulfide-based catalysts.
基金Supported by the National Natural Science Foundation of China （No.40671158）, the National 863 Program of China（No.2006AA12Z224） and the Program for New Century Excellent Talents in University （No.NCET-05-0626）.
文摘The problem of taking an unorganized point cloud in 3D space and fitting a polyhedral surface to those points is both important and difficult. Aiming at increasing applications of full three dimensional digital terrain surface modeling, a new algorithm for the automatic generation of three dimensional triangulated irregular network from a point cloud is pro- posed. Based on the local topological consistency test, a combined algorithm of constrained 3D Delaunay triangulation and region-growing is extended to ensure topologically correct reconstruction. This paper also introduced an efficient neighbor- ing triangle location method by making full use of the surface normal information. Experimental results prove that this algo- rithm can efficiently obtain the most reasonable reconstructed mesh surface with arbitrary topology, wherein the automati- cally reconstructed surface has only small topological difference from the true surface. This algorithm has potential applica- tions to virtual environments, computer vision, and so on.
基金Project supported by the National Natural Science Foundation of China(Nos.12372071 and 12372070)the Aeronautical Science Fund of China(No.2022Z055052001)the Foundation of China Scholarship Council(No.202306830079)。
文摘Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade components.In this paper,a dynamic model of 3D 4-directional braided composite thin plates considering braiding directions is established.Based on Kirchhoff's plate assumptions,the displacement variables of the plate are expressed.By incorporating the braiding directions into the constitutive equation of the braided composites,the dynamic model of the plate considering braiding directions is obtained.The effects of the speeds,braiding directions,and braided angles on the responses of the plate with fixed-axis rotation and translational motion,respectively,are investigated.This paper presents a dynamic theory for calculating the deformation of 3D braided composite structures undergoing both translational and rotational motions.It also provides a simulation method for investigating the dynamic behavior of non-isotropic material plates in various applications.
基金Project supported by the National Natural Science Foundation of China(Nos.W2433004 and 12472015)the Research Fund of the State Key Laboratory of Mechanics and Control of Mechanical Structures(Nanjing University of Aeronautics and Astronautics)(No.MCMS-I-0122K01).
文摘This paper proposes an attitude control strategy for a flexible satellite equipped with an orthogonal cluster of three-dimensional(3D)magnetically suspended wheels(MSWs).The mathematical model for the satellite incorporating flexible appendages and an orthogonal cluster of magnetically suspended reaction wheel actuators is initially developed.After that,an adaptive attitude controller is designed with a switching surface of variable structure,an adaptive law for estimating inertia matrix uncertainty,and a fuzzy disturbance observer for estimating disturbance torques.Additionally,a Moore-Penrose-based steering law is proposed to derive the tilt angle commands of the orthogonal configuration of the 3D MSW to follow the designed control signal.Finally,numerical simulations are presented to validate the effectiveness of the proposed control strategy.
文摘Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma.
