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Three-dimensional(3D)printing-assisted freeze-casting of processed pyritum-dopedβ-tricalcium phosphate biomimetic scaffold with angiogenesis and bone regeneration capability 被引量:1
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作者 Chenxu WEI Zongan LI +9 位作者 Xiaoyun LIANG Yuwei ZHAO Xingyu ZHU Haibing HUA Guobao CHEN Kunming QIN Zhipeng CHEN Changcan SHI Feng ZHANG Weidong LI 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 2025年第9期863-880,共18页
Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds ... Bone repair remains an important target in tissue engineering,making the development of bioactive scaffolds for effective bone defect repair a critical objective.In this study,β-tricalcium phosphate(β-TCP)scaffolds incorporated with processed pyritum decoction(PPD)were fabricated using three-dimensional(3D)printing-assisted freeze-casting.The produced composite scaffolds were evaluated for their mechanical strength,physicochemical properties,biocompatibility,in vitro proangiogenic activity,and in vivo efficacy in repairing rabbit femoral defects.They not only demonstrated excellent physicochemical properties,enhanced mechanical strength,and good biosafety but also significantly promoted the proliferation,migration,and aggregation of pro-angiogenic human umbilical vein endothelial cells(HUVECs).In vivo studies revealed that all scaffold groups facilitated osteogenesis at the bone defect site,with theβ-TCP scaffolds loaded with PPD markedly enhancing the expression of neurogenic locus Notch homolog protein 1(Notch1),vascular endothelial growth factor(VEGF),bone morphogenetic protein-2(BMP-2),and osteopontin(OPN).Overall,the scaffolds developed in this study exhibited strong angiogenic and osteogenic capabilities both in vitro and in vivo.The incorporation of PPD notably promoted the angiogenic-osteogenic coupling,thereby accelerating bone repair,which suggests that PPD is a promising material for bone repair and that the PPD/β-TCP scaffolds hold great potential as a bone graft alternative. 展开更多
关键词 Bone defect Processed pyritum three-dimensional(3d)printing-assisted freeze-casting ANGIOGENESIS Bone regeneration VEGF-Notch1-BMP-2-OPN coupling
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Advances and Prospects in Body-Size Measurement of Sheep:From 2D Vision to 3D Reconstruction and 2D-3D Fusion
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作者 DAI Weijiao LIANG Yudongchen +5 位作者 ZHOU Yong YAO Chao ZHANG Cheng SONG Yongjian LI Guoliang TIAN Fang 《智慧农业(中英文)》 2026年第1期120-147,共28页
[Significance]In alignment with the national germplasm security strategy,current research efforts are accelerating the adoption of precision breeding in sheep.Within the whole-genome selection,accurate phenotyping of ... [Significance]In alignment with the national germplasm security strategy,current research efforts are accelerating the adoption of precision breeding in sheep.Within the whole-genome selection,accurate phenotyping of body morphometrics is critical for assessing growth performance and breeding value.Traditional manual measurements are inefficient,prone to human error,and may cause stress to sheep,limiting their suitability for precision sheep management.By summarizing the applications of sheep body size measurement technologies and analyzing their development directions,this paper provides theoretical references and practical guidance for the research and application of non contact sheep body size measurement.[Progress]This review synthesizes progress across three principal methodological paradigms:two-dimensional(2D)image-based techniques,three-dimensional(3D)point cloud-based approaches,and integrated 2D-3D fusion systems.2D methods,employing either handcrafted geometric features or deep learning-based keypoint detector algorithms,are cost-effective and operationally simple but sensitive to variation in imaging conditions and unable to capture critical circumference metrics.3D point-cloud approaches enable precise reconstruction of full animal morphology,supporting comprehensive body-size acquisition with higher accuracy,yet face challenges including high hardware costs,complex data workflows,and sensitivity to posture variability.Hybrid 2D-3D fusion systems combine semantic richness from RGB imagery with geometric completeness from point clouds.Having been effectively validated in other livestock specise,e.g.,cattle and pigs,these fusion systems have demonstrated excellent performance,providing important technical references and practical insights for sheep body size measurement.[Conclusions and Prospects]Firstly,future research should focus on constructing large-scale,high-quality datasets for sheep body size measurement that encompass diverse breeds,growth stages,and environmental conditions,thereby enhancing model robustness and generalization.Secondly,the development of lightweight artificial intelligence models is essential.Techniques such as model compression,quantization,and algorithmic optimization can substantially reduce computational complexity and storage requirements,facilitating deployment in resource-constrained environments.Thirdly,the 3D point cloud processing pipeline should be streamlined to improve the efficiency of data acquisition,filtering,registration,and segmentation,while promoting the integration of low-cost,high-resilience vision systems into practical farming scenarios.Fourthly,specific emphasis should be placed on improving the accuracy of curved-dimensional measurements,such as chest circumference,abdominal circumference,and shank circumference,through advances in pose standardization,refined 3D segmentation strategies,and multimodal data fusion.Finally,the cross-fertilization of sheep body size measurement technologies with analogous methods for other livestock species offers a promising pathway for mutual learning and collaborative innovation,accelerating the industrialization of automated sheep morphometric systems and supporting the development of intelligent,data-driven pasture management practices. 展开更多
关键词 smart breeding computer vision image recognition three-dimensional reconstruction 2d-3d body measurement
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Effect of dominant fractures on triaxial behavior of 3D-printed rock analogs with internal fracture networks
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作者 Lishuai Jiang Pimao Li +3 位作者 Xin He Yang Zhao Quansen Wu Ye Zhao 《Journal of Rock Mechanics and Geotechnical Engineering》 2026年第2期1390-1412,共23页
Internal structural defects in engineering rock masses vary in size,exhibit complex shapes,and are unevenly distributed.Dominant fractures within a rock mass often play a critical to its mechanical behavior,directly a... Internal structural defects in engineering rock masses vary in size,exhibit complex shapes,and are unevenly distributed.Dominant fractures within a rock mass often play a critical to its mechanical behavior,directly affecting the macromechanical properties and failure modes.These fractures affect the instability and failure of the surrounding rock,significantlyimpacting the overall stability of engineering structures.Herein,sand-powder three-dimensional(3D)printing technology was used to prepare rock-like specimens with internal fracture networks.Triaxial compression testing,post-failure fracture mapping,and fractal dimension analysis of the fracture surfaces were conducted to investigate the effects of dominant fracture angles on the strength and deformation of rocks with internal fracture networks under triaxial stress.The results indicate that the dominant fracture angle has a pronounced effect on the mechanical behavior of rock.With increasing angle,both compressive strength and elastic modulus exhibit an initial decline followed by an increase.Moreover,higher confiningpressure significantlyimproves the compressive strength of fractured rock.This enhancement weakens as the confiningpressure further increases.Moreover,with increasing confiningpressure,the differences between the maximum and minimum values of elastic moduli and lateral strain ratios in fractured rock gradually decrease.Thus,the impact of the dominant fracture angle on rock mass deformation decreases with increasing confiningpressure.This research elucidates the effects of dominant fracture angles on the mechanical and failure properties of complex fractured rock masses and the influenceof the confiningpressure on these relationships.It provides valuable theoretical insights and practical guidance for stability analyses in engineering rock masses. 展开更多
关键词 Sand powder three-dimensional(3d) printing Internal fracture networks Triaxial compression Rock mechanics Fractal dimension
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Dynamic modeling of a three-dimensional braided composite thin plate considering braiding directions 被引量:1
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作者 Chentong GAO Huiyu SUN +1 位作者 Jianping GU W.M.HUANG 《Applied Mathematics and Mechanics(English Edition)》 2025年第1期123-138,共16页
Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade compone... Currently,there are a limited number of dynamic models available for braided composite plates with large overall motions,despite the incorporation of three-dimensional(3D)braided composites into rotating blade components.In this paper,a dynamic model of 3D 4-directional braided composite thin plates considering braiding directions is established.Based on Kirchhoff's plate assumptions,the displacement variables of the plate are expressed.By incorporating the braiding directions into the constitutive equation of the braided composites,the dynamic model of the plate considering braiding directions is obtained.The effects of the speeds,braiding directions,and braided angles on the responses of the plate with fixed-axis rotation and translational motion,respectively,are investigated.This paper presents a dynamic theory for calculating the deformation of 3D braided composite structures undergoing both translational and rotational motions.It also provides a simulation method for investigating the dynamic behavior of non-isotropic material plates in various applications. 展开更多
关键词 three-dimensional(3d)braided composite braiding direction composite thin plate large overall motion dynamic model
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Artificial intelligence-aided semi-automatic joint trace detection from textured three-dimensional models of rock mass 被引量:1
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作者 Seyedahmad Mehrishal Jineon Kim +1 位作者 Yulong Shao Jae Joon Song 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第4期1973-1985,共13页
It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimens... It is of great importance to obtain precise trace data,as traces are frequently the sole visible and measurable parameter in most outcrops.The manual recognition and detection of traces on high-resolution three-dimensional(3D)models are relatively straightforward but time-consuming.One potential solution to enhance this process is to use machine learning algorithms to detect the 3D traces.In this study,a unique pixel-wise texture mapper algorithm generates a dense point cloud representation of an outcrop with the precise resolution of the original textured 3D model.A virtual digital image rendering was then employed to capture virtual images of selected regions.This technique helps to overcome limitations caused by the surface morphology of the rock mass,such as restricted access,lighting conditions,and shading effects.After AI-powered trace detection on two-dimensional(2D)images,a 3D data structuring technique was applied to the selected trace pixels.In the 3D data structuring,the trace data were structured through 2D thinning,3D reprojection,clustering,segmentation,and segment linking.Finally,the linked segments were exported as 3D polylines,with each polyline in the output corresponding to a trace.The efficacy of the proposed method was assessed using a 3D model of a real-world case study,which was used to compare the results of artificial intelligence(AI)-aided and human intelligence trace detection.Rosette diagrams,which visualize the distribution of trace orientations,confirmed the high similarity between the automatically and manually generated trace maps.In conclusion,the proposed semi-automatic method was easy to use,fast,and accurate in detecting the dominant jointing system of the rock mass. 展开更多
关键词 Automatic trace detection digital joint mapping Rock discontinuities characterization three-dimensional(3d)trace network
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Three-dimensional models:from cell culture to Patient-Derived Organoid and its application to future liposarcoma research
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作者 SAYUMI TAHARA SYDNEY RENTSCH +4 位作者 FERNANDA COSTAS CASAL DE FARIA PATRICIA SARCHET ROMA KARNA FEDERICA CALORE RAPHAEL E.POLLOCK 《Oncology Research》 SCIE 2025年第1期1-13,共13页
Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma ... Liposarcoma is one of the most common soft tissue sarcomas,however,its occurrence rate is still rare compared to other cancers.Due to its rarity,in vitro experiments are an essential approach to elucidate liposarcoma pathobiology.Conventional cell culture-based research(2D cell culture)is still playing a pivotal role,while several shortcomings have been recently under discussion.In vivo,mouse models are usually adopted for pre-clinical analyses with expectations to overcome the issues of 2D cell culture.However,they do not fully recapitulate human dedifferentiated liposarcoma(DDLPS)characteristics.Therefore,three-dimensional(3D)culture systems have been the recent research focus in the cell biology field with the expectation to overcome at the same time the disadvantages of 2D cell culture and in vivo animal models and fill in the gap between them.Given the liposarcoma rarity,we believe that 3D cell culture techniques,including 3D cell cultures/co-cultures,and Patient-Derived tumor Organoids(PDOs),represent a promising approach to facilitate liposarcoma investigation and elucidate its molecular mechanisms and effective therapy development.In this review,we first provide a general overview of 3D cell cultures compared to 2D cell cultures.We then focus on one of the recent 3D cell culture applications,Patient-Derived Organoids(PDOs),summarizing and discussing several PDO methodologies.Finally,we discuss the current and future applications of PDOs to sarcoma,particularly in the field of liposarcoma. 展开更多
关键词 Cell culture LIPOSARCOMA Patient-derived Organoid(PdO) SPHEROId three-dimensional(3d)cell culture
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MXene-based 2D/2D Ti_(3)C_(2)/TiO_(2) heterojunction with spatially separate d re dox sites for efficient photocatalytic N_(2) reduction towards NH_(3) 被引量:1
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作者 Chi Cao Jinshuo Li +3 位作者 Linfeng Zhang Yang Hu Lin Zhang Wensheng Yang 《Journal of Materials Science & Technology》 2025年第11期180-193,共14页
Herein,a new type of two-dimensional(2D)/2D Ti_(3)C_(2)/TiO_(2) heterojunction was developed for efficient photocatalytic nitrogen reduction reaction(NRR),in which TiO_(2) nanosheets(TiO_(2) Ns)were designed as the ma... Herein,a new type of two-dimensional(2D)/2D Ti_(3)C_(2)/TiO_(2) heterojunction was developed for efficient photocatalytic nitrogen reduction reaction(NRR),in which TiO_(2) nanosheets(TiO_(2) Ns)were designed as the main catalyst,while Ti_(3)C_(2) MXene served as the co-catalyst.Experimental and theoretical results revealed that Ti_(3)C_(2) MXene introduced electron-rich unsaturated Ti sites,serving as highly active sites for both the adsorption and activation of N_(2) on the Ti_(3)C_(2)/TiO_(2) heterojunction.Furthermore,the 2D/2D Ti_(3)C_(2)/TiO_(2) heterostructure greatly promoted the directional separation and transfer of charge carriers,facilitated by the internal electric field.This structural feature enabled the spatial separation of the N_(2) reduction and H2 O oxidation half-reactions on the distinct surfaces of Ti_(3)C_(2)(001)and TiO_(2)(001),con-sequently reducing the reaction energy barrier for each respective process.The synergistic effects arising from the interface and surface interactions within the heterojunction conspicuously improved the photo-catalytic NRR activity.As a result,the optimized Ti_(3)C_(2)/TiO_(2) heterojunction exhibited a high NH_(3) produc-tion rate of 24.4μmol g−1 h−1 in the absence of sacrificial agents,representing a remarkable 12.8-fold increase compared to individual TiO_(2) Ns.This work provides new insights into rational design of high-performance heterogeneous photocatalysts and offers a deeper understanding of the mechanism under-lying surface active sites in the photocatalytic NRR process. 展开更多
关键词 2d/2d heterojunctionTi_(3)C_(2)MXene Photocatalytic N_(2)reduction reaction N_(2)adsorption and activation spatially separated redox sites
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Real-time model updating and prediction of three-dimensional timevarying consolidation settlement using machine learning
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作者 Huaming Tian Yu Wang Danni Zhang 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第9期5954-5969,共16页
The development of digital twins for geotechnical structures necessitates the real-time updates of threedimensional(3D)virtual models(e.g.numerical finite element method(FEM)model)to accurately predict time-varying ge... The development of digital twins for geotechnical structures necessitates the real-time updates of threedimensional(3D)virtual models(e.g.numerical finite element method(FEM)model)to accurately predict time-varying geotechnical responses(e.g.consolidation settlement)in a 3D spatial domain.However,traditional 3D numerical model updating approaches are computationally prohibitive and therefore difficult to update the 3D responses in real time.To address these challenges,this study proposes a novel machine learning framework called sparse dictionary learning(T-3D-SDL)for real-time updating of time-varying 3D geotechnical responses.In T-3D-SDL,a concerned dataset(e.g.time-varying 3D settlement)is approximated as a linear superposition of dictionary atoms generated from 3D random FEM analyses.Field monitoring data are then used to identify non-trivial atoms and estimate their weights within a Bayesian framework for model updating and prediction.The proposed approach enables the real-time update of temporally varying settlements with a high 3D spatial resolution and quantified uncertainty as field monitoring data evolve.The proposed approach is illustrated using an embankment construction project.The results show that the proposed approach effectively improves settlement predictions along temporal and 3D spatial dimensions,with minimal latency(e.g.within minutes),as monitoring data appear.In addition,the proposed approach requires only a reasonably small number of 3D FEM model evaluations,avoids the use of widely adopted yet often criticized surrogate models,and effectively addresses the limitations(e.g.computational inefficiency)of existing 3D model updating approaches. 展开更多
关键词 digital twin three-dimensional(3d)finite element method(FEM) Time-varying 3d settlement Real-time model update Sparse dictionary learning(SdL)
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Interacting bosons in a three-dimensional lattice
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作者 Dian-Cheng Zhang Shi-Jie Yang 《Chinese Physics B》 2025年第8期501-504,共4页
We theoretically investigate the extended Bose-Hubbard model using a three-dimensional cubic lattice.In the framework of the dynamical Gutzwiller mean-field theory,we identify a checkerboard supersolid phase.By consid... We theoretically investigate the extended Bose-Hubbard model using a three-dimensional cubic lattice.In the framework of the dynamical Gutzwiller mean-field theory,we identify a checkerboard supersolid phase.By considering the repulsive interactions between next-nearest-neighbor lattice sites,we further discover an exotic type of supersolid state,whose site occupancies show a stereoscopically arrayed and staggered distribution rather than checkerboard ordering.Intriguingly,if the physical observations of two neighboring layers were superimposed,they would give rise to a checkerboard configuration.This novel structure is convincingly induced by the simultaneous existence of nearest-neighbor and nextnearest-neighbor interactions.We also identify arrayed stripes in the ground state,as well as arrayed holes in the pattern of occupancies. 展开更多
关键词 SUPERSOLId three-dimensional(3d)optical lattice extended Bose-Hubbard dynamical Gutzwiller theory
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A human-machine interaction method for rock discontinuities mapping by three-dimensional point clouds with noises
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作者 Qian Chen Yunfeng Ge +3 位作者 Changdong Li Huiming Tang Geng Liu Weixiang Chen 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第3期1646-1663,共18页
Rock discontinuities control rock mechanical behaviors and significantly influence the stability of rock masses.However,existing discontinuity mapping algorithms are susceptible to noise,and the calculation results ca... Rock discontinuities control rock mechanical behaviors and significantly influence the stability of rock masses.However,existing discontinuity mapping algorithms are susceptible to noise,and the calculation results cannot be fed back to users timely.To address this issue,we proposed a human-machine interaction(HMI)method for discontinuity mapping.Users can help the algorithm identify the noise and make real-time result judgments and parameter adjustments.For this,a regular cube was selected to illustrate the workflows:(1)point cloud was acquired using remote sensing;(2)the HMI method was employed to select reference points and angle thresholds to detect group discontinuity;(3)individual discontinuities were extracted from the group discontinuity using a density-based cluster algorithm;and(4)the orientation of each discontinuity was measured based on a plane fitting algorithm.The method was applied to a well-studied highway road cut and a complex natural slope.The consistency of the computational results with field measurements demonstrates its good accuracy,and the average error in the dip direction and dip angle for both cases was less than 3.Finally,the computational time of the proposed method was compared with two other popular algorithms,and the reduction in computational time by tens of times proves its high computational efficiency.This method provides geologists and geological engineers with a new idea to map rapidly and accurately rock structures under large amounts of noises or unclear features. 展开更多
关键词 Rock discontinuities three-dimensional(3d)point clouds discontinuity identification Orientation measurement Human-machine interaction
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Attitude control of flexible satellite via three-dimensional magnetically suspended wheel
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作者 J.TAYEBI Yingjie CHEN +1 位作者 Ti CHEN Shiyuan JIA 《Applied Mathematics and Mechanics(English Edition)》 2025年第3期555-572,共18页
This paper proposes an attitude control strategy for a flexible satellite equipped with an orthogonal cluster of three-dimensional(3D)magnetically suspended wheels(MSWs).The mathematical model for the satellite incorp... This paper proposes an attitude control strategy for a flexible satellite equipped with an orthogonal cluster of three-dimensional(3D)magnetically suspended wheels(MSWs).The mathematical model for the satellite incorporating flexible appendages and an orthogonal cluster of magnetically suspended reaction wheel actuators is initially developed.After that,an adaptive attitude controller is designed with a switching surface of variable structure,an adaptive law for estimating inertia matrix uncertainty,and a fuzzy disturbance observer for estimating disturbance torques.Additionally,a Moore-Penrose-based steering law is proposed to derive the tilt angle commands of the orthogonal configuration of the 3D MSW to follow the designed control signal.Finally,numerical simulations are presented to validate the effectiveness of the proposed control strategy. 展开更多
关键词 flexible satellite three-dimensional(3d)magnetically suspended wheel(MSW) attitude control adaptive control disturbance observer
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The detection of keratoconus using a three-dimensional corneal model derived from anterior segment optical coherence tomography
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作者 Sang Ngoc Tran Isa S.K.Mohammed +1 位作者 Zeshan Tariq Wuqaas M.Munir 《Annals of Eye Science》 2025年第3期73-82,共10页
Background:Traditional imaging approaches to keratoconus(KCN)have thus far failed to produce a standardized approach for diagnosis.While many diagnostic modalities and metrics exist,none have proven robust enough to b... Background:Traditional imaging approaches to keratoconus(KCN)have thus far failed to produce a standardized approach for diagnosis.While many diagnostic modalities and metrics exist,none have proven robust enough to be considered a gold standard.This study aims to introduce novel metrics to differentiate between KCN and healthy corneas using three-dimensional(3D)measurements of surface area and volume.Methods:This retrospective observational study examined KCN patients along with healthy control patients between the ages of 20 and 79 years old at the University of Maryland,Baltimore.The selected patients underwent a nine-line raster scan anterior segment optical coherence tomography(AS-OCT).ImageJ was used to determine the central 6 mm of each image and each corneal image was then divided into six 1 mm segments.Free-D software was then used to render the nine different images into a 3D model to calculate corneal surface area and volume.A two-tailed Mann-Whitney test was used to assess statistical significance when comparing these subsets.Results:Thirty-three eyes with KCN,along with 33 healthy control,were enrolled.There were statistically significant differences between the healthy and KCN groups in the metric of anterior corneal surface area(13.927 vs.13.991 mm^(2),P=0.046),posterior corneal surface area(14.045 vs.14.173 mm^(2),P<0.001),and volume(8.430 vs.7.773 mm3,P<0.001)within the central 6 mm.Conclusions:3D corneal models derived from AS-OCT can be used to measure anterior corneal surface area,posterior corneal surface area,and corneal volume.All three parameters are statistically different between corneas with KCN and healthy corneas.Further study and application of these parameters may yield new methodologies for the detection of KCN. 展开更多
关键词 CORNEA ECTASIA keratoconus(KCN) anterior segment optical coherence tomography(AS-OCT) three-dimensional model(3d model)
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Three-dimensional gravity inversion based on 3D U-Net++ 被引量:4
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作者 Wang Yu-Feng Zhang Yu-Jie +1 位作者 Fu Li-Hua Li Hong-Wei 《Applied Geophysics》 SCIE CSCD 2021年第4期451-460,592,共11页
The gravity inversion is to restore genetic density distribution of the underground target to be explored for explaining the internal structure and distribution of the Earth.In this paper,we propose a new 3D gravity i... The gravity inversion is to restore genetic density distribution of the underground target to be explored for explaining the internal structure and distribution of the Earth.In this paper,we propose a new 3D gravity inversion method based on 3D U-Net++.Compared with two-dimensional gravity inversion,three-dimensional(3D)gravity inversion can more precisely describe the density distribution of underground space.However,conventional 3D gravity inversion method input is two-dimensional,the input and output of the network proposed in our method are three-dimensional.In the training stage,we design a large number of diversifi ed simulation model-data pairs by using the random walk method to improve the generalization ability of the network.In the test phase,we verify the network performance by using the model-data pairs generated by the simulation.To further illustrate the eff ectiveness of the algorithm,we apply the method to the inversion of the San Nicolas mining area,and the inversion results are basically consistent with the borehole measurement results.Moreover,the results of the 3D U-Net++inversion and the 3D U-Net inversion are compared.The density models of the 3D U-Net++inversion have higher resolution,more concentrated inversion results,and a clearer boundary of the density model. 展开更多
关键词 deep learning gravity anomaly three-dimensional gravity inversion 3d U-Net++
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A Three-Dimensional (3D) Environment to Maintain the Integrity of Mouse Testicular Can Cause the Occurrence of Meiosis 被引量:1
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作者 CHU Zhi-li LIU Chao +3 位作者 BAI Yao-fu ZHU Hai-jing HU Yue HUA Jin-lian 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第8期1481-1488,共8页
Adhesions between different cells and extracellular matrix have been studied extensively in vitro, but little is known about their functions in testicular tissue counterparts. Spermatogonia and their companion somatic... Adhesions between different cells and extracellular matrix have been studied extensively in vitro, but little is known about their functions in testicular tissue counterparts. Spermatogonia and their companion somatic cells maintain a close association throughout spermatogenesis and this association is necessary for normal spermatogenesis. In order to keep the relative integrity of the testicular tissues, and to detect the development in vitro, culture testicular tissues in a three- dimensional (3D) agarose matrix was examined. Testicular tissues isolated from 6.5 d postpartum (dpp) mouse were cultured on the top of the matrix for 26 d with a medium height up to 4/5 of the 3D agarose matrix. The results showed that in this 3D culture environment, each type of testicular cells kept the same structure, localization and function as in vivo and might be more biologically relevant to living organisms. After culture, germ cell marker VASA and meiosis markers DAZL and SCP3 showed typical positive analysed by immunofluorescence staining and RT-PCR. It demonstrated that this 3D culture system was able to maintain the number of germ cells and promote the meiosis initiation of male germ cells. 展开更多
关键词 three-dimensional culture 3d MEIOSIS organ culture MOUSE
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Three-dimensional(3D)parametric measurements of individual gravels in the Gobi region using point cloud technique
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作者 JING Xiangyu HUANG Weiyi KAN Jiangming 《Journal of Arid Land》 SCIE CSCD 2024年第4期500-517,共18页
Gobi spans a large area of China,surpassing the combined expanse of mobile dunes and semi-fixed dunes.Its presence significantly influences the movement of sand and dust.However,the complex origins and diverse materia... Gobi spans a large area of China,surpassing the combined expanse of mobile dunes and semi-fixed dunes.Its presence significantly influences the movement of sand and dust.However,the complex origins and diverse materials constituting the Gobi result in notable differences in saltation processes across various Gobi surfaces.It is challenging to describe these processes according to a uniform morphology.Therefore,it becomes imperative to articulate surface characteristics through parameters such as the three-dimensional(3D)size and shape of gravel.Collecting morphology information for Gobi gravels is essential for studying its genesis and sand saltation.To enhance the efficiency and information yield of gravel parameter measurements,this study conducted field experiments in the Gobi region across Dunhuang City,Guazhou County,and Yumen City(administrated by Jiuquan City),Gansu Province,China in March 2023.A research framework and methodology for measuring 3D parameters of gravel using point cloud were developed,alongside improved calculation formulas for 3D parameters including gravel grain size,volume,flatness,roundness,sphericity,and equivalent grain size.Leveraging multi-view geometry technology for 3D reconstruction allowed for establishing an optimal data acquisition scheme characterized by high point cloud reconstruction efficiency and clear quality.Additionally,the proposed methodology incorporated point cloud clustering,segmentation,and filtering techniques to isolate individual gravel point clouds.Advanced point cloud algorithms,including the Oriented Bounding Box(OBB),point cloud slicing method,and point cloud triangulation,were then deployed to calculate the 3D parameters of individual gravels.These systematic processes allow precise and detailed characterization of individual gravels.For gravel grain size and volume,the correlation coefficients between point cloud and manual measurements all exceeded 0.9000,confirming the feasibility of the proposed methodology for measuring 3D parameters of individual gravels.The proposed workflow yields accurate calculations of relevant parameters for Gobi gravels,providing essential data support for subsequent studies on Gobi environments. 展开更多
关键词 Gobi gravels three-dimensional(3d)parameters point cloud 3d reconstruction Random Sample Consensus(RANSAC)algorithm density-Based spatial Clustering of Applications with Noise(dBSCAN)
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A COMPUTER CODE FASTOR-3D FOR TRANSIENT THREE-DIMENSIONAL SIMULATION ON FLOW AND HEAT TRANS-FER WITH POROSITY APPROXIMATION
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作者 卢万成 邓保庆 席时桐 《Journal of Shanghai Jiaotong university(Science)》 EI 1997年第2期43-46,52,共5页
Upon the conservation of mass, momentum and energy, volume fraction and surface penetrative rate were employed to modify the conservative equations to simulate the effect of blockages on fluid flows and heat transfer.... Upon the conservation of mass, momentum and energy, volume fraction and surface penetrative rate were employed to modify the conservative equations to simulate the effect of blockages on fluid flows and heat transfer. These equations were solved numerically with the finite differential method and the primitive variable approach. This method uses staggered grid and pressure correction schemes. A computer code FASTOR3D integrated the aforementioned algorithm. The preliminary results have been compared with conventional benchmark solutions. With auxiliary software DV, the numerical results were visualized in colorful images to demonstrate the variation of flow patterns and temperature profiles during the transient process. The results of the simulation code for the fluid flows and heat transfer in the sodium pool of a fast breeder reactor are acceptable. 展开更多
关键词 three-dimensional SIMULATION FLOW and HEAT transfer POROSITY APPROXIMATION FASTOR3d
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Three-dimensional spatial multi-point uniform light focusing through scattering media based on feedback wavefront shaping
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作者 Fan Yang Yang Zhao +2 位作者 Chengchao Xiang Qi Feng Yingchun Ding 《Chinese Physics B》 SCIE EI CAS CSCD 2021年第4期264-270,共7页
We use feedback wavefront shaping technology to realize the multi-point uniform light focusing in three-dimensional(3D) space through scattering media only by loading the optimal mask once.General 3D spatial focusing ... We use feedback wavefront shaping technology to realize the multi-point uniform light focusing in three-dimensional(3D) space through scattering media only by loading the optimal mask once.General 3D spatial focusing needs to load the optimal mask multiple times to realize the spatial movement of the focal point and the uniformity of multi-point focusing cannot be guaranteed.First,we investigate the effects of speckle axial correlation and different axial distances on 3D spatial multi-point uniform focusing and propose possible solutions.Then we use our developed non-dominated sorting genetic algorithm suitable for 3D spatial focusing(S-NSGA) to verify the experiment of multi-point focusing in 3D space.This research is expected to have potential applications in the fields of optical manipulation and optogenetics. 展开更多
关键词 wavefront shaping speckle correlation 3d spatial focusing multi-point focusing
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Three-Dimensional Ocean Sensor Networks:A Survey 被引量:21
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作者 WANG Yu LIU Yingjian GUO Zhongwen 《Journal of Ocean University of China》 SCIE CAS 2012年第4期436-450,共15页
The past decade has seen a growing interest in ocean sensor networks because of their wide applications in marine research,oceanography,ocean monitoring,offshore exploration,and defense or homeland security.Ocean sens... The past decade has seen a growing interest in ocean sensor networks because of their wide applications in marine research,oceanography,ocean monitoring,offshore exploration,and defense or homeland security.Ocean sensor networks are generally formed with various ocean sensors,autonomous underwater vehicles,surface stations,and research vessels.To make ocean sensor network applications viable,efficient communication among all devices and components is crucial.Due to the unique characteristics of underwater acoustic channels and the complex deployment environment in three dimensional(3D) ocean spaces,new efficient and reliable communication and networking protocols are needed in design of ocean sensor networks.In this paper,we aim to provide an overview of the most recent advances in network design principles for 3D ocean sensor networks,with focuses on deployment,localization,topology design,and position-based routing in 3D ocean spaces. 展开更多
关键词 ocean sensor networks underwater sensor networks three-dimensional sensor networks ocean applications 3d de-ployment topology design LOCALIZATION position-based routing
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GTP-based Integral Real-3D Spatial Model for Engineering Excavation GIS 被引量:11
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作者 WULixin SHIWenzhong 《Geo-Spatial Information Science》 2004年第2期123-128,共6页
Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP mo... Engineering excavation GIS (E 2 GIS) is a real-3D GIS serving for geosciences related to geo-engineering, civil engineering and mining engineering based on generalized tri-prism (GTP) model. As two instances of GTP model, G\|GTP is used for the real\|3D modeling of subsurface geological bodies, and E\|GTP is used for the real\|3D modeling of subsurface engineering excavations.In the light of the discussions on the features and functions of E 2 GIS, the modeling principles of G\|GTP and E\|GTP are introduced. The two models couple together seamlessly to form an integral model for subsurface spatial objects including both geological bodies and excavations. An object\|oriented integral real\|3D data model and integral spatial topological relations are discussed. 展开更多
关键词 real-3d spatial modeling GEOSCIENCES engineering GTP model E^2 GIS
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Effects of dry-wet cycles on three-dimensional pore structure and permeability characteristics of granite residual soil using X-ray micro computed tomography 被引量:26
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作者 Ran An Lingwei Kong +1 位作者 Xianwei Zhang Chengsheng Li 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2022年第3期851-860,共10页
Due to seasonal climate alterations,the microstructure and permeability of granite residual soil are easily affected by multiple dry-wet cycles.The X-ray micro computed tomography(micro-CT)acted as a nondestructive to... Due to seasonal climate alterations,the microstructure and permeability of granite residual soil are easily affected by multiple dry-wet cycles.The X-ray micro computed tomography(micro-CT)acted as a nondestructive tool for characterizing the microstructure of soil samples exposed to a range of damage levels induced by dry-wet cycles.Subsequently,the variations of pore distribution and permeability due to drywet cycling effects were revealed based on three-dimensional(3D)pore distribution analysis and seepage simulations.According to the results,granite residual soils could be separated into four different components,namely,pores,clay,quartz,and hematite,from micro-CT images.The reconstructed 3D pore models dynamically demonstrated the expanding and connecting patterns of pore structures during drywet cycles.The values of porosity and connectivity are positively correlated with the number of dry-wet cycles,which were expressed by exponential and linear functions,respectively.The pore volume probability distribution curves of granite residual soil coincide with the χ^(2)distribution curve,which verifies the effectiveness of the assumption of χ^(2)distribution probability.The pore volume distribution curves suggest that the pores in soils were divided into four types based on their volumes,i.e.micropores,mesopores,macropores,and cracks.From a quantitative and visual perspective,considerable small pores are gradually transformed into cracks with a large volume and a high connectivity.Under the action of dry-wet cycles,the number of seepage flow streamlines which contribute to water permeation in seepage simulation increases distinctly,as well as the permeability and hydraulic conductivity.The calculated hydraulic conductivity is comparable with measured ones with an acceptable error margin in general,verifying the accuracy of seepage simulations based on micro-CT results. 展开更多
关键词 Granite residual soil dry-wet cycle X-ray micro computed tomography(micro-CT) three-dimensional(3d)pore distribution Seepage simulations PERMEABILITY
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