Thigmotropism and thigmomorphogenesis are two related and pervasive processes that play crucial roles in plant adaptation to the environment.However,there have been few investigations into the molecular regulatory mec...Thigmotropism and thigmomorphogenesis are two related and pervasive processes that play crucial roles in plant adaptation to the environment.However,there have been few investigations into the molecular regulatory mechanisms of these phenomena.Cucumber(Cucumis sativus L.)tendrils are ideal material for studying thigmotropism and thigmomorphogenesis because they display a combination of the two processes.Here,we generated the transcriptome profiles of cucumber tendrils at the young,stretch,and coiling stages.Genes related to receptor proteins,transmembrane transport,and ion transport were significantly enriched among those differentially expressed between stages.Pharmacological assays illustrated that three GLUTAMATE RECEPTOR(GLR)genes might play a vital function in perceiving or transducing touch stimulation signals.Comparing the transcriptomes of tendrils and roots after touch stimulation,we found that genes related to extracellular stimulus and xyloglucan metabolism might have conserved functions in the regulation of thigmomorphogenesis.The transcriptome atlas of thigmotropism and thigmomorphogenesis of cucumber tendrils constructed in this study will help further elucidate the molecular mechanisms behind these processes.展开更多
This study investigated the influence of both shaking duration and number of trees per bale on postharvest needle characteristics such as percentage needle loss, needle retention duration and explored the physiologica...This study investigated the influence of both shaking duration and number of trees per bale on postharvest needle characteristics such as percentage needle loss, needle retention duration and explored the physiological roles of endogenous ethylene and volatile terpene compounds (VTCs). To accomplish these objectives, 25 six-year-old trees were detached and exposed to a range of shaking durations (0 to 60 sec.), and 30 six-year-old detached trees were exposed to baling treatments from 0 to 5 trees. Response variables measured were percent needle loss, needle retention duration, average water use, ethylene and volatile terpene compound evolution. Trees shaken for 60 seconds lost 16% less needle compared to control, which was consistent with the decrease in percent needle loss with increasing shaking duration. Baled trees lost 13% more needles compared to control, but percent needle loss was observed to decrease with increasing number of trees in a bale. These trends corresponded with increasing ethylene and VTC evolutions, where the longer the shaking duration or larger number of trees in a bale, the higher the ethylene and VTC evolutions. One can therefore draw inference that mechanical perturbation as a result of shaking and baling induce biosynthesis and regulation of ethylene and VTC in balsam fir trees in an effort to regulate postharvest needle abscission.展开更多
基金National Key R&D Program of China(Grant No.2019YFA0906200)the Science and Technology Innovation Team of Shaanxi(Grant No.2021TD-32)the Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(Grant Nos.CAAS-ASTIP and CAAS-ZDRW202009).
文摘Thigmotropism and thigmomorphogenesis are two related and pervasive processes that play crucial roles in plant adaptation to the environment.However,there have been few investigations into the molecular regulatory mechanisms of these phenomena.Cucumber(Cucumis sativus L.)tendrils are ideal material for studying thigmotropism and thigmomorphogenesis because they display a combination of the two processes.Here,we generated the transcriptome profiles of cucumber tendrils at the young,stretch,and coiling stages.Genes related to receptor proteins,transmembrane transport,and ion transport were significantly enriched among those differentially expressed between stages.Pharmacological assays illustrated that three GLUTAMATE RECEPTOR(GLR)genes might play a vital function in perceiving or transducing touch stimulation signals.Comparing the transcriptomes of tendrils and roots after touch stimulation,we found that genes related to extracellular stimulus and xyloglucan metabolism might have conserved functions in the regulation of thigmomorphogenesis.The transcriptome atlas of thigmotropism and thigmomorphogenesis of cucumber tendrils constructed in this study will help further elucidate the molecular mechanisms behind these processes.
文摘This study investigated the influence of both shaking duration and number of trees per bale on postharvest needle characteristics such as percentage needle loss, needle retention duration and explored the physiological roles of endogenous ethylene and volatile terpene compounds (VTCs). To accomplish these objectives, 25 six-year-old trees were detached and exposed to a range of shaking durations (0 to 60 sec.), and 30 six-year-old detached trees were exposed to baling treatments from 0 to 5 trees. Response variables measured were percent needle loss, needle retention duration, average water use, ethylene and volatile terpene compound evolution. Trees shaken for 60 seconds lost 16% less needle compared to control, which was consistent with the decrease in percent needle loss with increasing shaking duration. Baled trees lost 13% more needles compared to control, but percent needle loss was observed to decrease with increasing number of trees in a bale. These trends corresponded with increasing ethylene and VTC evolutions, where the longer the shaking duration or larger number of trees in a bale, the higher the ethylene and VTC evolutions. One can therefore draw inference that mechanical perturbation as a result of shaking and baling induce biosynthesis and regulation of ethylene and VTC in balsam fir trees in an effort to regulate postharvest needle abscission.
