Myocardial damage resulting from acute myocardial infarction often leads to progressive heart failure and sudden death,highlighting the urgent clinical need for effective therapies.Recently,tanshinoneⅡA has been iden...Myocardial damage resulting from acute myocardial infarction often leads to progressive heart failure and sudden death,highlighting the urgent clinical need for effective therapies.Recently,tanshinoneⅡA has been identified as a promising therapeutic agent for myocardial infarction.However,efficient delivery remains a major issue that limits clinical translation.To address this problem,an injectable thermosensitive poly(lactic acid-co-glycolic acid)-block-poly(ethylene glycol)-block-poly(lactic acid-co-glycolic acid)gel(PLGA-PEG-PLGA)system encapsulating tanshinoneⅡA-loaded reactive oxygen species-sensitive microspheres(Gel-MS/tanshinoneⅡA)has been designed and synthesized in this study.The thermosensitive hydrogel exhibits good mechanical properties after reaching body temperature.Microspheres initially immobilized by the gel exhibit excellent reactive oxygen species-triggered release properties in a high-reactive oxygen species environment after myocardial infarction onset.As a result,encapsulated tanshinoneⅡA is effectively released into the infarcted myocardium,where it exerts local anti-pyroptotic and anti-inflammatory effects.Importantly,the combined advantages of this technique contribute to the mitigation of left ventricular remodeling and the restoration of cardiac function following tanshinoneⅡA.Therefore,this novel,precision-guided intra-tissue therapeutic system allows for customized local release of tanshinoneⅡA,presenting a promising alternative treatment strategy aimed at inducing beneficial ventricular remodeling in the post-infarct heart.展开更多
Nowadays,there is a growing concern among consumers about the impact of diet on health.In this regard,oleogelation has been proposed as a promising strategy to replace unhealthy fats,as well as to encapsulate water-in...Nowadays,there is a growing concern among consumers about the impact of diet on health.In this regard,oleogelation has been proposed as a promising strategy to replace unhealthy fats,as well as to encapsulate water-insoluble bioactive compounds in food matrices.Consequently,the development of thermoreversible oleogels may play an important role in the bioavailability and biostability of functional foods and the beneficial effects associated with them.The aim of this work was to evaluate the viscoelastic behavior of oleogels using a commercial food additive(E471)composed of fatty acid monoglycerides(MG).Furthermore,the study of sol-gel transitions of these thermoreversible oleogels allowed to obtain the optimum concentration of gelling agent required.Refined sunflower oil containing 2-5 wt%of E471 were characterized by means of SAOS tests,as a function of temperature.All these systems were liquids above 48℃,forming stable oleogels below 20℃.An improvement of the elastic component of them was observed as gelling agent concentration was higher.Oleogels containing 4 and 5 wt%MG showed very similar viscoelastic properties and high stability at body temperature(ca.37℃).Conversely,systems containing 2%or 3%led to the formation of weaker gels exhibiting thermo-reversibility but only the latter was stable at body temperature.Therefore,sunflower oleogels containing MG above 3 wt%could be regarded as potential carrier food matrices for lipid-soluble active ingredients.To this end,the modulation of the oleogel network consistency should play a key role in maintaining the integrity of the active principle during gastrointestinal transit.展开更多
基金supported by the National Natural Science Foundation of China(82104962,82104647,82274271)Scientific Research Project of Guangdong Provincial Administration of Traditional Chinese Medicine(20211070)+2 种基金Science and Technology Planning Project of Guangzhou(202102010301)Young Talents Support Project from China Association of Chinese Medicine(2019-QNRC2-C06)Team of Prevention and Treatment of Acute Myocardial Infarction with Chinese Medicine(2019KCXTD009)
文摘Myocardial damage resulting from acute myocardial infarction often leads to progressive heart failure and sudden death,highlighting the urgent clinical need for effective therapies.Recently,tanshinoneⅡA has been identified as a promising therapeutic agent for myocardial infarction.However,efficient delivery remains a major issue that limits clinical translation.To address this problem,an injectable thermosensitive poly(lactic acid-co-glycolic acid)-block-poly(ethylene glycol)-block-poly(lactic acid-co-glycolic acid)gel(PLGA-PEG-PLGA)system encapsulating tanshinoneⅡA-loaded reactive oxygen species-sensitive microspheres(Gel-MS/tanshinoneⅡA)has been designed and synthesized in this study.The thermosensitive hydrogel exhibits good mechanical properties after reaching body temperature.Microspheres initially immobilized by the gel exhibit excellent reactive oxygen species-triggered release properties in a high-reactive oxygen species environment after myocardial infarction onset.As a result,encapsulated tanshinoneⅡA is effectively released into the infarcted myocardium,where it exerts local anti-pyroptotic and anti-inflammatory effects.Importantly,the combined advantages of this technique contribute to the mitigation of left ventricular remodeling and the restoration of cardiac function following tanshinoneⅡA.Therefore,this novel,precision-guided intra-tissue therapeutic system allows for customized local release of tanshinoneⅡA,presenting a promising alternative treatment strategy aimed at inducing beneficial ventricular remodeling in the post-infarct heart.
基金part of the project PID2022-142663OB-100,funded by MICIU/AEI/10.13039/501100011033by ERDF/EU,as well as the project PY20_01046,funded by the‘Consejería de Economía,Conocimiento,Investigacion y Universidad de la Junta de Andalucía/FEDER’.
文摘Nowadays,there is a growing concern among consumers about the impact of diet on health.In this regard,oleogelation has been proposed as a promising strategy to replace unhealthy fats,as well as to encapsulate water-insoluble bioactive compounds in food matrices.Consequently,the development of thermoreversible oleogels may play an important role in the bioavailability and biostability of functional foods and the beneficial effects associated with them.The aim of this work was to evaluate the viscoelastic behavior of oleogels using a commercial food additive(E471)composed of fatty acid monoglycerides(MG).Furthermore,the study of sol-gel transitions of these thermoreversible oleogels allowed to obtain the optimum concentration of gelling agent required.Refined sunflower oil containing 2-5 wt%of E471 were characterized by means of SAOS tests,as a function of temperature.All these systems were liquids above 48℃,forming stable oleogels below 20℃.An improvement of the elastic component of them was observed as gelling agent concentration was higher.Oleogels containing 4 and 5 wt%MG showed very similar viscoelastic properties and high stability at body temperature(ca.37℃).Conversely,systems containing 2%or 3%led to the formation of weaker gels exhibiting thermo-reversibility but only the latter was stable at body temperature.Therefore,sunflower oleogels containing MG above 3 wt%could be regarded as potential carrier food matrices for lipid-soluble active ingredients.To this end,the modulation of the oleogel network consistency should play a key role in maintaining the integrity of the active principle during gastrointestinal transit.
