Modern thermoelectric modules have emerged as promising platforms for precision thermal analysis in biological and chemical applications.This study presents a high-throughput microcalorimeter employing a patterned bis...Modern thermoelectric modules have emerged as promising platforms for precision thermal analysis in biological and chemical applications.This study presents a high-throughput microcalorimeter employing a patterned bismuth telluride(Bi_(2)Te_(3))thermopile array as integrated heat flux sensors,overcoming the throughput limitations of conventional calorimetric systems.Through finite element analysis-guided device optimization,we established that increasing thermocouple height from 0.4mm to 0.8mm reduces thermal conductance,achieving around 1 V·W^(-1)power sensitivity.The system demonstrated dual-mode calibration methods using both the electrical(Joule heating)and the chemical(water-ethanol mixing enthalpy)references.Device functionality was validated through real-time monitoring of Escherichia coli metabolism,revealing distinct thermal signatures upon antibiotic challenge.The antimicrobial susceptibility testing(AST)is performed with 4 commonly used antibiotics.The platform achieved 4 h AST with coherent values to Clinical and Laboratory Standards Institute(CLSI)guidelines for minimum inhibitory concentration(MIC)determination.Notably,the modular chip architecture integrates 8 sensing units as a proof-ofconcept,coupled with disposable microfluidic chambers that eliminate cross-contamination risks.This chipcalorimeter implementation establishes a new paradigm for chemical reaction heat measurement and rapid clinical diagnostics of infectious diseases.展开更多
基金supported by National Natural Science Foundation of China No.62203055China Postdoctoral Science Foundation No.2023M730232.
文摘Modern thermoelectric modules have emerged as promising platforms for precision thermal analysis in biological and chemical applications.This study presents a high-throughput microcalorimeter employing a patterned bismuth telluride(Bi_(2)Te_(3))thermopile array as integrated heat flux sensors,overcoming the throughput limitations of conventional calorimetric systems.Through finite element analysis-guided device optimization,we established that increasing thermocouple height from 0.4mm to 0.8mm reduces thermal conductance,achieving around 1 V·W^(-1)power sensitivity.The system demonstrated dual-mode calibration methods using both the electrical(Joule heating)and the chemical(water-ethanol mixing enthalpy)references.Device functionality was validated through real-time monitoring of Escherichia coli metabolism,revealing distinct thermal signatures upon antibiotic challenge.The antimicrobial susceptibility testing(AST)is performed with 4 commonly used antibiotics.The platform achieved 4 h AST with coherent values to Clinical and Laboratory Standards Institute(CLSI)guidelines for minimum inhibitory concentration(MIC)determination.Notably,the modular chip architecture integrates 8 sensing units as a proof-ofconcept,coupled with disposable microfluidic chambers that eliminate cross-contamination risks.This chipcalorimeter implementation establishes a new paradigm for chemical reaction heat measurement and rapid clinical diagnostics of infectious diseases.
