Eleutheronema Tetradactylum is commercially important marine fish and a fixture at tables.However,nonnegligible hazardous substances,such as heterocyclic aromatic amines(HAAs)and advanced glycation end products(AGEs),...Eleutheronema Tetradactylum is commercially important marine fish and a fixture at tables.However,nonnegligible hazardous substances,such as heterocyclic aromatic amines(HAAs)and advanced glycation end products(AGEs),generates along during thermal processing.Herein,we determined HAAs and two representative AGEs,Nε-carboxyethyl-lysine(CEL)and Nε-carboxymethyl-lysine(CML)formation in roasted Eleutheronema Tetradactylum,and proposed inhibitory method of tea polyphenol to effectively reduce HAAs,CML and CEL,which caused 51.23,46.72,24.06 and 35.48 percentage reduction of Norharman,Harman,CML and CEL,respectively,led IQ,8-MeIQx below detected limitation.Precursors(amino acids,creatin(in)e,glucose and glyoxal),lipid oxidation,fatty acid analysis and further nontargeted lipidomic approaches are attempted to reveal the inhibition mechanism.This work develops a new method to minimize HAAs and AGEs in Eleutheronema Tetradactylum,provide a comprehensive interpretation of inhibition mechanism by tea polyphenols,which is helpful to food manufacturers for healthier fish production.展开更多
基金financially supported by National Key Research and Development Program of China(2023YFD2100200)Dalian High-level Talent Innovation Support Program(2022RQ003).
文摘Eleutheronema Tetradactylum is commercially important marine fish and a fixture at tables.However,nonnegligible hazardous substances,such as heterocyclic aromatic amines(HAAs)and advanced glycation end products(AGEs),generates along during thermal processing.Herein,we determined HAAs and two representative AGEs,Nε-carboxyethyl-lysine(CEL)and Nε-carboxymethyl-lysine(CML)formation in roasted Eleutheronema Tetradactylum,and proposed inhibitory method of tea polyphenol to effectively reduce HAAs,CML and CEL,which caused 51.23,46.72,24.06 and 35.48 percentage reduction of Norharman,Harman,CML and CEL,respectively,led IQ,8-MeIQx below detected limitation.Precursors(amino acids,creatin(in)e,glucose and glyoxal),lipid oxidation,fatty acid analysis and further nontargeted lipidomic approaches are attempted to reveal the inhibition mechanism.This work develops a new method to minimize HAAs and AGEs in Eleutheronema Tetradactylum,provide a comprehensive interpretation of inhibition mechanism by tea polyphenols,which is helpful to food manufacturers for healthier fish production.
文摘于2015年6月~9月在广东珠海对人工孵化和培育的2龄四指马鲅(Eleutheronema tetradactylum)亲鱼的初次性成熟和产卵情况以及苗种规模化全人工繁育技术进行了系统的观察和研究。结果显示,实验期间培育亲鱼2 000余尾,成熟率93%以上。成熟亲鱼的体质量雌雄之间差异较大,雌鱼性腺指数(GSI)为1.03%~6.05%,雄鱼GSI为0.26%~1.76%。雌鱼产卵类型为一年产卵1次,分批成熟、分批产卵,初次性成熟的产卵期从2015年6月13日~9月30日。共计产卵75.0×106粒,受精率为72%~95%,胚胎发育在盐度27~28.8、温度29~31℃、p H 8.1~8.3条件下历时13 h 52 min,孵化率为58%~95%。仔鱼在室内培育,投喂小球藻和轮虫,5~7 d后转移到室外土池进行培育,7 d后投喂小球藻、轮虫、枝角类和桡足类幼体。第25天起投喂卤虫无节幼体,第30天起投喂粉状配合饲料并逐渐过渡到投喂人工颗粒饲料。从2015年7月23日开始至9月30日累计培育出子二代苗15.0×106尾,平均成活率为25.0%。综合亲鱼产卵和幼苗育成情况,认为该鱼全人工繁育的技术关键在于如何培育优质的亲鱼以及育苗过程中水质的控制和适口饵料选择及其适时更换。
