TEM and SHV are among the most prevalentβ-lactamases contributing toβ-lactam antibiotic resistance in clinical settings,leading to treatment challenges and increased mortality rates.Except for penicillin and early c...TEM and SHV are among the most prevalentβ-lactamases contributing toβ-lactam antibiotic resistance in clinical settings,leading to treatment challenges and increased mortality rates.Except for penicillin and early cephalosporins,TEM and SHV variants have evolved with the ability to hydrolyze the second-and third-generation cephalosporins,monobactams,and evenβ-lactamase inhibitors.Accurate detection ofβ-lactamases is of paramount importance for optimizing antibiotic use and combating antimicrobial resistance(AMR).While genetic detection methods,such as polymerase chain reaction(PCR),are widely employed,their positive results may lack phenotypic correlation due to the low or absent expression of blaSHV and blaTEM in many strains[1].Therefore,a direct protein-level detection method such as targeted proteomics is more precise and clinically relevant.This study highlights the development of a rapid detection method using targeted proteomics with high-resolution accurate mass(HRAM)Orbitrap MS for the direct detection of TEM and SHV in Enterobacteriaceae strains,which offers greater clinical relevance compared to conventional genetic approaches.展开更多
The corrosion behavior of 304LN austenitic stainless steel in supercritical CO_(2) at 650℃ was investigated.The results show that 304LN follows Wagner’s law kinetics,forming a protective oxide flm consisting of SiO_...The corrosion behavior of 304LN austenitic stainless steel in supercritical CO_(2) at 650℃ was investigated.The results show that 304LN follows Wagner’s law kinetics,forming a protective oxide flm consisting of SiO_(2),(Cr,Mn)3O_(4),and Cr2O_(3) from the inner to outer layers.A shallow carburization depth of approximately 130 nm indicates excellent resistance to carburization.The roles of key elements in 18/8 austenitic stainless steel represented by 304LN,such as Cr,Ni,and Si,were analyzed,highlighting their contributions to anti-carburization performance and corrosion resistance under harsh conditions.展开更多
1.Introduction Microbiologically influenced corrosion(MIC)is the destruction of metal materials caused by the activity of microorganisms and the participation of biofilms[1].Global economic costs caused by marine corr...1.Introduction Microbiologically influenced corrosion(MIC)is the destruction of metal materials caused by the activity of microorganisms and the participation of biofilms[1].Global economic costs caused by marine corrosion come to hundreds of billion dollars per year,with approximately 20% of corrosion losses caused by MIC[2].The MIC poses a serious threat to the integrity and safety of assets in the oil and gas industry,water industry,and nuclear waste storage facili-ties[3-5].展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.:32141003,82330110,and 81803593)the CAMS Innovation Fund for Medical Sciences(CIFMS),China(Grant Nos.:2021-I2M-1-030,and 2021-I2M-1-039)+1 种基金the Fundamental Research Funds for the Central Universities,China(Grant No.:3332018094)the National Science and Technology Infrastructure of China(Project No.:National Pathogen Resource Center-NPRC-32).
文摘TEM and SHV are among the most prevalentβ-lactamases contributing toβ-lactam antibiotic resistance in clinical settings,leading to treatment challenges and increased mortality rates.Except for penicillin and early cephalosporins,TEM and SHV variants have evolved with the ability to hydrolyze the second-and third-generation cephalosporins,monobactams,and evenβ-lactamase inhibitors.Accurate detection ofβ-lactamases is of paramount importance for optimizing antibiotic use and combating antimicrobial resistance(AMR).While genetic detection methods,such as polymerase chain reaction(PCR),are widely employed,their positive results may lack phenotypic correlation due to the low or absent expression of blaSHV and blaTEM in many strains[1].Therefore,a direct protein-level detection method such as targeted proteomics is more precise and clinically relevant.This study highlights the development of a rapid detection method using targeted proteomics with high-resolution accurate mass(HRAM)Orbitrap MS for the direct detection of TEM and SHV in Enterobacteriaceae strains,which offers greater clinical relevance compared to conventional genetic approaches.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDA0410000)the CAS Project for Young Scientists in Basic Research(No.YSBR-043)+1 种基金the CNNC Science Fund for Talented Young Scholars,the National Funding Program for Postdoctoral Researchers(GZC20232747)the Youth Innovation Promotion Association CAS(2022187).
文摘The corrosion behavior of 304LN austenitic stainless steel in supercritical CO_(2) at 650℃ was investigated.The results show that 304LN follows Wagner’s law kinetics,forming a protective oxide flm consisting of SiO_(2),(Cr,Mn)3O_(4),and Cr2O_(3) from the inner to outer layers.A shallow carburization depth of approximately 130 nm indicates excellent resistance to carburization.The roles of key elements in 18/8 austenitic stainless steel represented by 304LN,such as Cr,Ni,and Si,were analyzed,highlighting their contributions to anti-carburization performance and corrosion resistance under harsh conditions.
基金supported by the National Natural Science Foun-dation of China(Nos.52371071,51971228,and 51771212).
文摘1.Introduction Microbiologically influenced corrosion(MIC)is the destruction of metal materials caused by the activity of microorganisms and the participation of biofilms[1].Global economic costs caused by marine corrosion come to hundreds of billion dollars per year,with approximately 20% of corrosion losses caused by MIC[2].The MIC poses a serious threat to the integrity and safety of assets in the oil and gas industry,water industry,and nuclear waste storage facili-ties[3-5].
