Tear fluid,also referred to as tears or tear film,is an important biological fluid that plays a key role in maintaining ocular surface health and immune homeostasis.Recent studies have found that tear fluid not only p...Tear fluid,also referred to as tears or tear film,is an important biological fluid that plays a key role in maintaining ocular surface health and immune homeostasis.Recent studies have found that tear fluid not only participates in the occurrence and development of ocular diseases,but also exerts profound effects in the immune pathological mechanisms of systemic diseases,breaking through the inherent understanding previously held by the scientific community.Immune cells in tear fluid(such as T cells,neutrophils,natural killer cells,macrophages),cytokines,and immunoglobulins can specifically participate in autoimmune diseases(such as Sjögren’s syndrome,rheumatoid arthritis,systemic lupus erythematosus,multiple sclerosis,Graves’ophthalmopathy)and systemic diseases(such as Alzheimer’s disease,diabetes mellitus,graft-versus-host disease).The dynamic changes in tear fluid components can reflect systemic immune homeostasis imbalance.Tear fluid biomarkers,such as exosomal microRNA(miR)-204,miR-200b-5p,and the protein markerβ2-microglobulin,have shown great potential in early disease screening,diagnostic stratification,and therapeutic target discovery.Tear fluid immune component analysis may provide innovative diagnostic tools and therapeutic targets for systemic diseases.Future research should focus on promoting the standardization and clinical transformation of tear fluid testing technologies and their clinical application.展开更多
目的:观察成功准分子激光角膜磨镶术(laser-assisted in situ keratomileusis,LASIK)术后人眼泪液中基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)的长期变化。方法:分别收集32例64眼LASIK手术的近视患者术前、术后15d;1,3mo;1a...目的:观察成功准分子激光角膜磨镶术(laser-assisted in situ keratomileusis,LASIK)术后人眼泪液中基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)的长期变化。方法:分别收集32例64眼LASIK手术的近视患者术前、术后15d;1,3mo;1a的泪液,进行Western-blot实验,检测MMP-2浓度的变化。结果:LASIK手术导致术后患者泪液中MMP-2浓度增加。术后15d,MMP-2约为术前的1.4倍,随着术后时间的延长逐渐下降,术后3mo仍未恢复(P<0.05),术后1a几乎恢复至术前水平(P>0.05)。结论:LASIK手术增加人眼泪液中MMP-2的浓度,且随着术后时间的延长逐渐下降,术后3mo仍未恢复,术后1a恢复至术前水平,表明LASIK诱发的创伤修复反应至少持续3mo,术后1a几乎恢复。展开更多
基金supported by the Medical Scientific Research Foundation of Guangdong Province,China(A2023423)。
文摘Tear fluid,also referred to as tears or tear film,is an important biological fluid that plays a key role in maintaining ocular surface health and immune homeostasis.Recent studies have found that tear fluid not only participates in the occurrence and development of ocular diseases,but also exerts profound effects in the immune pathological mechanisms of systemic diseases,breaking through the inherent understanding previously held by the scientific community.Immune cells in tear fluid(such as T cells,neutrophils,natural killer cells,macrophages),cytokines,and immunoglobulins can specifically participate in autoimmune diseases(such as Sjögren’s syndrome,rheumatoid arthritis,systemic lupus erythematosus,multiple sclerosis,Graves’ophthalmopathy)and systemic diseases(such as Alzheimer’s disease,diabetes mellitus,graft-versus-host disease).The dynamic changes in tear fluid components can reflect systemic immune homeostasis imbalance.Tear fluid biomarkers,such as exosomal microRNA(miR)-204,miR-200b-5p,and the protein markerβ2-microglobulin,have shown great potential in early disease screening,diagnostic stratification,and therapeutic target discovery.Tear fluid immune component analysis may provide innovative diagnostic tools and therapeutic targets for systemic diseases.Future research should focus on promoting the standardization and clinical transformation of tear fluid testing technologies and their clinical application.
文摘目的:观察成功准分子激光角膜磨镶术(laser-assisted in situ keratomileusis,LASIK)术后人眼泪液中基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)的长期变化。方法:分别收集32例64眼LASIK手术的近视患者术前、术后15d;1,3mo;1a的泪液,进行Western-blot实验,检测MMP-2浓度的变化。结果:LASIK手术导致术后患者泪液中MMP-2浓度增加。术后15d,MMP-2约为术前的1.4倍,随着术后时间的延长逐渐下降,术后3mo仍未恢复(P<0.05),术后1a几乎恢复至术前水平(P>0.05)。结论:LASIK手术增加人眼泪液中MMP-2的浓度,且随着术后时间的延长逐渐下降,术后3mo仍未恢复,术后1a恢复至术前水平,表明LASIK诱发的创伤修复反应至少持续3mo,术后1a几乎恢复。
