白先勇是誉满海内外的当代华语文学的领军人物,生命悲剧书写是他始终不渝的创作取向。“Danny Boy”和“Tea for Two”两部小说关注生活在艾滋病肆虐背景下的美国纽约华人同性恋群体,在挖掘他们面对命运不公的孤独、无奈及自我救赎的同...白先勇是誉满海内外的当代华语文学的领军人物,生命悲剧书写是他始终不渝的创作取向。“Danny Boy”和“Tea for Two”两部小说关注生活在艾滋病肆虐背景下的美国纽约华人同性恋群体,在挖掘他们面对命运不公的孤独、无奈及自我救赎的同时,也隐含对美国社会现实的批判。小说运用疾病的隐喻、音乐的象征等艺术手法,含蓄地表达自己的情感态度和文化立场,是白先勇人道主义情怀和人文主义精神的升华之作;且作为20世纪七八十年代美国纽约华人世界那段不堪回首历史的文学镜像,颇具认识价值和启示意义。白先勇的悲悯情怀源自他对世界和人生的深刻生命体验及由此形成的生命悲剧意识,源于童年经验、人生经历、文化接受及认同危机等多方面影响。展开更多
Materials exhibiting broadband nonlinear optical responses are critically important for ultrafast photonics applications,particularly as saturable absorbers(SAs)that facilitate broadband optical pulse generation.In th...Materials exhibiting broadband nonlinear optical responses are critically important for ultrafast photonics applications,particularly as saturable absorbers(SAs)that facilitate broadband optical pulse generation.In this study,tea polyphenolpolyvinyl alcohol(TP-PVA)composite films are synthesized via a polymer embedding method and employed as SAs to initiate ultrafast pulse operation in fiber lasers.The TP-PVA SA film exhibits excellent broadband saturable absorption performance at wavelengths of 1.0μm,1.5μm,and 2.0μm,with modulation depths of 54.21%,41.41%,and 51.16%,respectively.Stable passively mode-locked pulses with pulse widths of 588 fs,419 fs,and 743 fs are generated in Yb-,Er-,and Tm-doped fiber lasers,respectively.This work confirms the effective performance of TP-PVA as a broadband SA,and establishes a foundation for the integration of novel and sustainable materials within ultrafast photonic systems.The approach paves the way for developing compact broadband ultrafast laser systems operating in the near-infrared spectral region.展开更多
As the source and main producing area of tea in the world, China has formed unique tea culture, and achievedremarkable economic benefits. However, frequent meteorological disasters, particularly low temperature frostd...As the source and main producing area of tea in the world, China has formed unique tea culture, and achievedremarkable economic benefits. However, frequent meteorological disasters, particularly low temperature frostdamage in late spring has seriously threatened the growth status of tea trees and caused quality and yield reduction of tea industry. Thus, timely and accurate early warning of frost damage occurrence in specific tea garden isvery important for tea plantation management and economic values. Aiming at the problems existing in currentmeteorological disaster forecasting methods, such as difficulty in obtaining massive meteorological data, largeamount of calculation for predicted models and incomplete information on frost damage occurrence, this paperproposed a two-fold algorithm for short-term and real-time prediction of temperature using field environmentaldata, and temperature trend results from a nearest local weather station for accurate frost damage occurrence leveldetermination, so as to achieve a specific tea garden frost damage occurrence prediction in a microclimate. Timeseries meteorological data collected from a small weather station was used for testing and parameterization of atwo-fold method, and another dataset acquired from Tea Experimental Base of Zhejiang University was furtherused to validate the capability of a two-fold model for frost damage forecasting. Results showed that comparedwith the results of autoregressive integrated moving average (ARIMA) and multiple linear regression (MLR),the proposed two-fold method using a second order Furrier fitting model and a K-Nearest Neighbor model(K = 3) with three days historical temperature data exhibited excellent accuracy for frost damage occurrence prediction on consideration of both model accuracy and computation (98.46% forecasted duration of frost damage,and 95.38% for forecasted temperature at the onset time). For field test in a tea garden, the proposed methodaccurately predicted three times frost damage occurrences, including onset time, duration and occurrence level.These results suggested the newly-proposed two-fold method was suitable for tea plantation frost damage occurrence forecasting.展开更多
Once a mining village,Jingshan in Hangzhou has reinvented itself over the past 20 years as a thriving model of green transformation.STROLLING through a village named Jingshan located in Hangzhou City,east China’s Zhe...Once a mining village,Jingshan in Hangzhou has reinvented itself over the past 20 years as a thriving model of green transformation.STROLLING through a village named Jingshan located in Hangzhou City,east China’s Zhejiang Province,one will notice the image of a“little tea farmer”wearing a bamboo hat and carrying a tea basket in many places.展开更多
Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibili...Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibility of constructing an optimal industrialization operation system driven by the dual wheels of"branding+standardization".The article first clarifies the connotation of high-quality development and the synergistic mechanism between branding and standardization.It then analyzes the current situation and bottlenecks of China's tea industry development.Subsequently,it proposes a dual-wheel drive strategy where branding enhances value and standardization guarantees quality,and designs a systematic implementation plan involving industrial chain synergy optimization and integrated support from government,industry,academia,research,and application.On this basis,strategies and suggestions are proposed,encompassing the starting point,standard focal points,key effort areas,innovation points,and target achievement points.The aim is to promote the tea industry to break through homogeneous competition,achieve value ascent,and provide important industrial support for regional high-quality development through the construction of the aforementioned system.展开更多
The water conservancy construction and soil and water conservation projects in New China have reshaped the tea industry landscape in Xinyang City of Henan Province from multiple dimensions.In terms of hydraulic techno...The water conservancy construction and soil and water conservation projects in New China have reshaped the tea industry landscape in Xinyang City of Henan Province from multiple dimensions.In terms of hydraulic technology,engineering systems represented by the Nanwan Irrigation District redefined the watershed irrigation pattern,while the application of modern agricultural technologies such as sprinkler and electric irrigation accelerated the shift from traditional experience to scientific management in tea garden practices.Ecologically,systematic soil and water management significantly improved growing conditions,making it possible for tea cultivation to break through the traditional geographical limitation of"tea does not grow north of the Huai River."This integrated governance model not only laid the foundation for the northward expansion of Xinyang's tea industry across the Huai River but also facilitated the transition toward scale and intensification through improved irrigation infrastructure and the breeding of cold-resistant tea varieties.Ultimately,driven by the tripartite interactive mechanism of"engineering governance-ecological improvement-spatial expansion,"the tea industry in Xinyang achieved a historic leap from traditional production to modern intensive development.展开更多
The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacter...The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacterial efficacy remains a formidable challenge.In this context,we proposed a novel strategy that leverages swelling and erosion mechanisms to facilitate drug release of drug-loaded ultrahigh molecular weight polyethylene(UHMWPE),thereby ensuring its long-lasting antibacterial performance.Polyethylene oxide(PEO),a hydrophilic polymer with fast hydrating ability and high swelling capacity,was incorporated in UHMWPE alongside the antibacterial tea polyphenol(epigallocatechin gallate,EGCG as representative).The swelling of PEO enhanced water infiltration into the matrix,while the erosion of PEO balanced the release of the encapsulated EGCG,resulting in a steady release.The behavior was supported by the EGCG release profiles and the corresponding fitted release kinetic models.As demonstrated by segmented antibacterial assessments,the antibacterial efficiency was enhanced 2to 3 times in the PEO/EGCG/UHMWPE composite compared to that of EGCG/UHMWPE.Additionally,the PEO/EGCG/UHMWPE composite exhibited favorable biocompatibility and mechanical performance,making it a potential candidate for the development of drug-releasing joint implants to combat prosthetic bacterial infections.展开更多
BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves ...BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.展开更多
This study explores the reform and innovation of experimental and practical teaching for the Teochew Gongfu Tea course within the framework of intangible cultural heritage(ICH)education.Through the construction of a&q...This study explores the reform and innovation of experimental and practical teaching for the Teochew Gongfu Tea course within the framework of intangible cultural heritage(ICH)education.Through the construction of a"Three-Dimensional Synergy,Four-Competency Progression,and Five-Integration"model,the research aims to establish a comprehensive and sustainable system that integrates ICH inheritance,technical training,and innovation education.Drawing on Outcome-Based Education(OBE)and constructivism learning theory,the reform introduces multi-source collaboration,hybrid learning,and competence-oriented assessment mechanisms.Through case-based teaching and iterative feedback,the model effectively enhances students cultural cognition,technical proficiency,creative thinking,and professional development.The findings demonstrate that this model not only improves students practical performance and engagement but also deepens their understanding and appreciation of traditional culture,offering a replicable model for other ICH-related programs in higher education.展开更多
Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations b...Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations by formulating a double nano-emulsion(W/O/W)system incorporating macadamia oil and tea tree oil,using homogenization and phase inversion temperature(PIT)techniques.Comprehensive physicochemical charac-terization,including droplet size,polydispersity index(PDI),zeta potential,turbidity,Fourier transform infrared spectroscopy(FTIR),and SEM,was conducted alongside stability assessments under varying pH,temperature,and storage conditions.The optimized nano-emulsions exhibited nanoscale droplet sizes(10-40 nm),low PDI values(indicating high uniformity),and robust stability.Interestingly,the formulation with 2%W/O loading,with a particle size of 11.57 nm and a PDI of 0.04,demonstrated an antioxidant capacity of 4622.62μg ascorbic acid equivalents(AA)/g,which was significantly higher(p<0.05)compared to both natural oils(macadamia oil:20.91μg AA/g,tea tree oil:16.86μg AA/g)and a 10%Vitamin C aqueous solution(592.94μg AA/g).FTIR analysis confirmed the molecular integrity of Vitamin C and its successful encapsulation with macadamia and tea tree oils,while SEM images revealed uniformly spherical and well-dispersed droplets.Moreover,the formulation retained its structural integrity and antioxidant functionality under diverse pH and thermal conditions.These findings underscore the potential of double nano-emulsion systems to overcome the stability challenges of Vitamin C,offering a promising approach to enhance its bioavailability and therapeutic performance in phar-maceutical and cosmetic applications.展开更多
[Objectives]This study was conducted to establish a detection method for the simultaneous determination of 18 perfluorinated compounds(PFCs)in milk tea by ultra-high performance liquid chromatography-tandem mass spect...[Objectives]This study was conducted to establish a detection method for the simultaneous determination of 18 perfluorinated compounds(PFCs)in milk tea by ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).[Methods]The samples were first subjected to precipitation of proteins by potassium ferrocyanide solution and zinc acetate solution,and then extracted by acetonitrile and detected by LC-MS/MS.Next,sodium chloride was added,and after vortex centrifugation,the acetonitrile layer was dried by blowing with nitrogen.Subsequently,1 ml of methanol was added to prepare a test solution.An ACQUITY UPLC BEH C18(2.6 m,2.1 mm×100 mm)chromatographic column was used for liquid phase separation,and gradient elution was performed using 10 mmol ammonium acetate solution-acetonitrile as the mobile phase.The MS detection adopted the MRM mode for acquisition,positive and negative ion mode switching for simultaneous determination,and external standard method for quantification.[Results]In the linear ranges of the target substances,the correlation coefficients R^(2)were all greater than 0.99.The detection limits of the method was in the range of 0.001-0.05μg/kg,and the quantitation limits were in the range of 0.03-0.20 ng/L.The recovery values ranged from 72.8%to 110.5%.[Conclusions]This method has high sensitivity and good accuracy,and thus strong practical value.展开更多
On the stone-paved lanes of Songyang County that date back to ancient times,morning mist lingered as a faint fragrance of tea wafted from a century-old house.Inside,Yang Junjie,a tea maker born in the 1980s,worked def...On the stone-paved lanes of Songyang County that date back to ancient times,morning mist lingered as a faint fragrance of tea wafted from a century-old house.Inside,Yang Junjie,a tea maker born in the 1980s,worked deftly at the stove,his hands moving swiftly over the scorching iron wok as tender green tea leaves dance between his fingers.展开更多
Vine tea is documented in ancient Chinese books as having the function of promoting blood circulation.However,its effects and mechanisms remain unclear.The aim of this study was to comprehensively investigate the prot...Vine tea is documented in ancient Chinese books as having the function of promoting blood circulation.However,its effects and mechanisms remain unclear.The aim of this study was to comprehensively investigate the protective potential and mechanisms of vine tea in high-fat diet rat through a combination of in vivo and in vitro experiments.The efficacy of vine tea was evaluated using a high-fat diet rat model and an oxidized low-density lipoprotein-treated cell model,with physiological and biochemical indicators measured in rat serum and cell supernatants.Transcriptomics was utilized to investigate alterations mRNA expression following the administration of dihydromyricetin in cell model.Metabolomics and 16S rRNA sequencing was employed to examine changes in metabolites in the serum and changes in gut microbiota of high-fat diet rats after administering vine tea extract.Vine tea extract and dihydromyricetin can reduce elevated levels of lipids,including total cholesterol and triglycerides,following modeling.Transcriptomic data indicate that dihydromyricetin exerts its effects by regulating ferroptosis signaling pathways.Metabolomic analysis demonstrates that the administration of vine tea extract influences the vitamin K cycle and glutathione,thereby alleviating the progression of ferroptosis.Additionally,16S rRNA sequencing reveals that vine tea extract increases Lactobacillaceae in the gut microbiota,which subsequently affects the levels of lysophosphatidylcholine,a major phospholipid component of oxidized low-density lipoprotein in serum.Our results indicate that vine tea can regulate ferroptosis signaling pathways and increase Lactobacillaceae in the gut microbiota,thereby exhibiting cardiovascular protective effects in high-fat diet rats.展开更多
Understanding the molecular responses of tea leaves to mechanical stress is crucial for elucidating the mechanisms of post-harvest quality formation during oolong tea processing.This study employed an integrated multi...Understanding the molecular responses of tea leaves to mechanical stress is crucial for elucidating the mechanisms of post-harvest quality formation during oolong tea processing.This study employed an integrated multi-omics strategy to characterize the changes and interactions among metabolomic(MB),transcriptomic(TX),and proteomic(PT)profiles in mechanically stressed tea leaves.Mechanical stress initially activated damage-associated molecular patterns(DAMPs),including Ca^(2+)signaling,jasmonic acid signaling,and glutathione metabolism pathways.These processes subsequently induced quality-related metabolic pathways(QRMPs),particularly α-linolenic acid and phenylalanine metabolism.Upregulated expression of LOX,ADH1,and PAR genes,together with the increased abundance of their encoded proteins,respectively promoted the accumulation of jasmine lactone,benzyl alcohol,and 2-phenylethanol.These findings indicate that mechanical stress influences the metabolite biosynthesis in tea leaves through coordinated molecular responses.This study provides new insights into the molecular mechanisms underlying tea leaf responses to mechanical stress and a foundation for future investigations into how early molecular events may contribute to post-harvest metabolic changes during oolong tea processing.展开更多
Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved i...Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.展开更多
May 21,2024 marks the 5th.International Tea Day.On May 23,the Chinese Embassy in the UK held the 2024 Tea for Harmony Yaji Cultural Salon.At the event,vips were treated with three Hubei speciality teas,namely Enshi ...May 21,2024 marks the 5th.International Tea Day.On May 23,the Chinese Embassy in the UK held the 2024 Tea for Harmony Yaji Cultural Salon.At the event,vips were treated with three Hubei speciality teas,namely Enshi Yulu green tea,Yihong black tea,and Green Brick team,introduced to the history and culture of Chinese tea.展开更多
A simple and accurate high-performance liquid chromatography（HPLC）method to generate the fingerprints of crude Pu-erh tea（CPT）and ripened Pu-erh tea（RPT）is described.A suitable chromatographic system was establi...A simple and accurate high-performance liquid chromatography（HPLC）method to generate the fingerprints of crude Pu-erh tea（CPT）and ripened Pu-erh tea（RPT）is described.A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.6% formic acid as the mobile phase and a detection wavelength of 300 nm.HPLC analysis identified 24 common peaks among RPT and 21 common peaks among CPT.This study revealed that crude Pu-erh tea and ripened Pu-erh tea contained some similar major constituents,but with distinctive peaks,which may be caused by the difference in the fermentation process.This HPLC fingerprint method could be used to evaluate and authenticate crude Pu-erh tea and ripened Pu-erh tea.展开更多
[Objective] This study was conducted to select a batch of new high-effi- cient low-toxicity high-selectivity pesticides for the application in tea gardens. [Method] This experiment was designed with 12 treatments incl...[Objective] This study was conducted to select a batch of new high-effi- cient low-toxicity high-selectivity pesticides for the application in tea gardens. [Method] This experiment was designed with 12 treatments including clear water as the CK, in randomized block arrangement, with 4 replications, and each plot had an area of 30 m2. The pesticides were sprayed in the high occurrence period of larvae of tea lesser leafhopper when tea shoots grew vigorously. [Result] In the 11 pesti- cides, 100 g/L bifenthrin EC , 70% imidacloprid WDG and 150 g/L indoxacarb EC were the best pesticides with efficacies above 90%; and in the bio-pesticides, 0.5% azadirachtin SC showed an efficacy over 87.97%. [Conclusions] Among the tested pesticides, 100 g/L bifenthrin EC , 70% imidacloprid WDG and 150 g/L indoxacarb EC could effectively control tea lesser leafhopper during the outbreak of tea lesser leafhopper, and when the occurrence degree of tea lesser leafhopper is below the medium level, environment-friendly 0.5% azadirachtin SC, 0.5% veratrine SC, 0.3% matrine SC and 4.0×10^10 spores/g Beauveria bassiana OD could be selected.展开更多
文摘白先勇是誉满海内外的当代华语文学的领军人物,生命悲剧书写是他始终不渝的创作取向。“Danny Boy”和“Tea for Two”两部小说关注生活在艾滋病肆虐背景下的美国纽约华人同性恋群体,在挖掘他们面对命运不公的孤独、无奈及自我救赎的同时,也隐含对美国社会现实的批判。小说运用疾病的隐喻、音乐的象征等艺术手法,含蓄地表达自己的情感态度和文化立场,是白先勇人道主义情怀和人文主义精神的升华之作;且作为20世纪七八十年代美国纽约华人世界那段不堪回首历史的文学镜像,颇具认识价值和启示意义。白先勇的悲悯情怀源自他对世界和人生的深刻生命体验及由此形成的生命悲剧意识,源于童年经验、人生经历、文化接受及认同危机等多方面影响。
基金supported by the Opening Foundation of Hubei Key Laboratory for New Textile Materials and Applications Research(Grant No.FZXCL202410)the Key Project of Science and Technology Research Program of Hubei Provincial Department of Education,China(Grant No.D20231704)+1 种基金Wuhan Textile University(Grant No.523058)the Foundation of Wuhan Textile University(Grant No.K24058)。
文摘Materials exhibiting broadband nonlinear optical responses are critically important for ultrafast photonics applications,particularly as saturable absorbers(SAs)that facilitate broadband optical pulse generation.In this study,tea polyphenolpolyvinyl alcohol(TP-PVA)composite films are synthesized via a polymer embedding method and employed as SAs to initiate ultrafast pulse operation in fiber lasers.The TP-PVA SA film exhibits excellent broadband saturable absorption performance at wavelengths of 1.0μm,1.5μm,and 2.0μm,with modulation depths of 54.21%,41.41%,and 51.16%,respectively.Stable passively mode-locked pulses with pulse widths of 588 fs,419 fs,and 743 fs are generated in Yb-,Er-,and Tm-doped fiber lasers,respectively.This work confirms the effective performance of TP-PVA as a broadband SA,and establishes a foundation for the integration of novel and sustainable materials within ultrafast photonic systems.The approach paves the way for developing compact broadband ultrafast laser systems operating in the near-infrared spectral region.
基金Zhejiang Public Welfare Program of Applied Research(LGN19D010001)the National Key R&D Program of China(2019YFE0125300)+1 种基金Zhejiang Provincial Natural Science Foundation of China under Grant No.LGN19F030001Zhejiang Agricultural Cooperative and Extensive Project of Key Technology(2020XTTGCY04-02).
文摘As the source and main producing area of tea in the world, China has formed unique tea culture, and achievedremarkable economic benefits. However, frequent meteorological disasters, particularly low temperature frostdamage in late spring has seriously threatened the growth status of tea trees and caused quality and yield reduction of tea industry. Thus, timely and accurate early warning of frost damage occurrence in specific tea garden isvery important for tea plantation management and economic values. Aiming at the problems existing in currentmeteorological disaster forecasting methods, such as difficulty in obtaining massive meteorological data, largeamount of calculation for predicted models and incomplete information on frost damage occurrence, this paperproposed a two-fold algorithm for short-term and real-time prediction of temperature using field environmentaldata, and temperature trend results from a nearest local weather station for accurate frost damage occurrence leveldetermination, so as to achieve a specific tea garden frost damage occurrence prediction in a microclimate. Timeseries meteorological data collected from a small weather station was used for testing and parameterization of atwo-fold method, and another dataset acquired from Tea Experimental Base of Zhejiang University was furtherused to validate the capability of a two-fold model for frost damage forecasting. Results showed that comparedwith the results of autoregressive integrated moving average (ARIMA) and multiple linear regression (MLR),the proposed two-fold method using a second order Furrier fitting model and a K-Nearest Neighbor model(K = 3) with three days historical temperature data exhibited excellent accuracy for frost damage occurrence prediction on consideration of both model accuracy and computation (98.46% forecasted duration of frost damage,and 95.38% for forecasted temperature at the onset time). For field test in a tea garden, the proposed methodaccurately predicted three times frost damage occurrences, including onset time, duration and occurrence level.These results suggested the newly-proposed two-fold method was suitable for tea plantation frost damage occurrence forecasting.
文摘Once a mining village,Jingshan in Hangzhou has reinvented itself over the past 20 years as a thriving model of green transformation.STROLLING through a village named Jingshan located in Hangzhou City,east China’s Zhejiang Province,one will notice the image of a“little tea farmer”wearing a bamboo hat and carrying a tea basket in many places.
基金Supported by General Project of Philosophy and Social Sciences Research in Universities of Jiangsu Province,2024(2024SJYB1650).
文摘Based on the requirements of local high-quality economic development and addressing the critical task of transformation and upgrading in the tea industry,this paper systematically discusses the necessity and feasibility of constructing an optimal industrialization operation system driven by the dual wheels of"branding+standardization".The article first clarifies the connotation of high-quality development and the synergistic mechanism between branding and standardization.It then analyzes the current situation and bottlenecks of China's tea industry development.Subsequently,it proposes a dual-wheel drive strategy where branding enhances value and standardization guarantees quality,and designs a systematic implementation plan involving industrial chain synergy optimization and integrated support from government,industry,academia,research,and application.On this basis,strategies and suggestions are proposed,encompassing the starting point,standard focal points,key effort areas,innovation points,and target achievement points.The aim is to promote the tea industry to break through homogeneous competition,achieve value ascent,and provide important industrial support for regional high-quality development through the construction of the aforementioned system.
文摘The water conservancy construction and soil and water conservation projects in New China have reshaped the tea industry landscape in Xinyang City of Henan Province from multiple dimensions.In terms of hydraulic technology,engineering systems represented by the Nanwan Irrigation District redefined the watershed irrigation pattern,while the application of modern agricultural technologies such as sprinkler and electric irrigation accelerated the shift from traditional experience to scientific management in tea garden practices.Ecologically,systematic soil and water management significantly improved growing conditions,making it possible for tea cultivation to break through the traditional geographical limitation of"tea does not grow north of the Huai River."This integrated governance model not only laid the foundation for the northward expansion of Xinyang's tea industry across the Huai River but also facilitated the transition toward scale and intensification through improved irrigation infrastructure and the breeding of cold-resistant tea varieties.Ultimately,driven by the tripartite interactive mechanism of"engineering governance-ecological improvement-spatial expansion,"the tea industry in Xinyang achieved a historic leap from traditional production to modern intensive development.
基金the National Natural Science Foundation of China(No.5220031085)the Postdoctoral Research Project in Henan Province(No.HN2022054)+2 种基金the Key Scientific Research Projects of Colleges and Universities in Henan Province(No.23A430009)the State Key Laboratory of Polymer Materials Engineering(No.sklpme2022-4-03)the Key Technologies R&D Program of Henan Province(No.242102230131)。
文摘The considerable hazard posed by periprosthetic joint infections underlines the urgent need for the rapid advancement of in-situ drug delivery systems within joint materials.However,the pursuit of sustained antibacterial efficacy remains a formidable challenge.In this context,we proposed a novel strategy that leverages swelling and erosion mechanisms to facilitate drug release of drug-loaded ultrahigh molecular weight polyethylene(UHMWPE),thereby ensuring its long-lasting antibacterial performance.Polyethylene oxide(PEO),a hydrophilic polymer with fast hydrating ability and high swelling capacity,was incorporated in UHMWPE alongside the antibacterial tea polyphenol(epigallocatechin gallate,EGCG as representative).The swelling of PEO enhanced water infiltration into the matrix,while the erosion of PEO balanced the release of the encapsulated EGCG,resulting in a steady release.The behavior was supported by the EGCG release profiles and the corresponding fitted release kinetic models.As demonstrated by segmented antibacterial assessments,the antibacterial efficiency was enhanced 2to 3 times in the PEO/EGCG/UHMWPE composite compared to that of EGCG/UHMWPE.Additionally,the PEO/EGCG/UHMWPE composite exhibited favorable biocompatibility and mechanical performance,making it a potential candidate for the development of drug-releasing joint implants to combat prosthetic bacterial infections.
文摘BACKGROUND Periodontal disease is a chronic inflammatory condition characterized by periodontal pocket formation,clinical attachment loss,and destruction of alveolar bone.Its conventional treatment primarily involves mechanical debridement and plaque control,but localized antimicrobial therapy offers site-specific advantages.While antibiotics such as metronidazole and doxycycline are commonly used,green tea extract,which is rich in epigallocatechin gallate,has been proposed as a promising alternative for local drug delivery due to its anti-inflammatory and antimicrobial properties.AIM To compare the clinical efficacy of green tea extract gel and ornidazole gel as adjuncts to scaling and root planing(SRP)in patients with periodontitis.METHODS Teeth with probing pocket depths(PPD)of 4-7 mm were selected.Participants’baseline oral hygiene index-simplified,plaque index,clinical attachment loss,and PPD were recorded.The participants were randomized into two groups:One received green tea extract gel after SRP,and the other received ornidazole gel.Subgingival drug delivery was performed,and participants refrained from brushing or interproximal cleaning for ten days.Their clinical parameters were re-evaluated after one month.RESULTS The PPD decreased significantly from baseline to one month in both groups.However,the green tea extract gel group exhibited superior outcomes to the ornidazole gel group,with a mean difference in PPD of 0.28±0.78 mm at one month(P<0.007).CONCLUSION As an adjunct to SRP,green tea extract gel showed greater efficacy in improving clinical periodontal parameters than ornidazole gel.
文摘This study explores the reform and innovation of experimental and practical teaching for the Teochew Gongfu Tea course within the framework of intangible cultural heritage(ICH)education.Through the construction of a"Three-Dimensional Synergy,Four-Competency Progression,and Five-Integration"model,the research aims to establish a comprehensive and sustainable system that integrates ICH inheritance,technical training,and innovation education.Drawing on Outcome-Based Education(OBE)and constructivism learning theory,the reform introduces multi-source collaboration,hybrid learning,and competence-oriented assessment mechanisms.Through case-based teaching and iterative feedback,the model effectively enhances students cultural cognition,technical proficiency,creative thinking,and professional development.The findings demonstrate that this model not only improves students practical performance and engagement but also deepens their understanding and appreciation of traditional culture,offering a replicable model for other ICH-related programs in higher education.
基金Ho Chi Minh City University of Technology(HCMUT),VNU-HCM for supporting this study.
文摘Vitamin C,a potent antioxidant with broad therapeutic applications,is limited by rapid degradation under environmental stressors,which compromises its stability and bioactivity.This study addresses these limitations by formulating a double nano-emulsion(W/O/W)system incorporating macadamia oil and tea tree oil,using homogenization and phase inversion temperature(PIT)techniques.Comprehensive physicochemical charac-terization,including droplet size,polydispersity index(PDI),zeta potential,turbidity,Fourier transform infrared spectroscopy(FTIR),and SEM,was conducted alongside stability assessments under varying pH,temperature,and storage conditions.The optimized nano-emulsions exhibited nanoscale droplet sizes(10-40 nm),low PDI values(indicating high uniformity),and robust stability.Interestingly,the formulation with 2%W/O loading,with a particle size of 11.57 nm and a PDI of 0.04,demonstrated an antioxidant capacity of 4622.62μg ascorbic acid equivalents(AA)/g,which was significantly higher(p<0.05)compared to both natural oils(macadamia oil:20.91μg AA/g,tea tree oil:16.86μg AA/g)and a 10%Vitamin C aqueous solution(592.94μg AA/g).FTIR analysis confirmed the molecular integrity of Vitamin C and its successful encapsulation with macadamia and tea tree oils,while SEM images revealed uniformly spherical and well-dispersed droplets.Moreover,the formulation retained its structural integrity and antioxidant functionality under diverse pH and thermal conditions.These findings underscore the potential of double nano-emulsion systems to overcome the stability challenges of Vitamin C,offering a promising approach to enhance its bioavailability and therapeutic performance in phar-maceutical and cosmetic applications.
基金Supported by Natural Science Foundation of Hunan Province(2024JJ8266).
文摘[Objectives]This study was conducted to establish a detection method for the simultaneous determination of 18 perfluorinated compounds(PFCs)in milk tea by ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).[Methods]The samples were first subjected to precipitation of proteins by potassium ferrocyanide solution and zinc acetate solution,and then extracted by acetonitrile and detected by LC-MS/MS.Next,sodium chloride was added,and after vortex centrifugation,the acetonitrile layer was dried by blowing with nitrogen.Subsequently,1 ml of methanol was added to prepare a test solution.An ACQUITY UPLC BEH C18(2.6 m,2.1 mm×100 mm)chromatographic column was used for liquid phase separation,and gradient elution was performed using 10 mmol ammonium acetate solution-acetonitrile as the mobile phase.The MS detection adopted the MRM mode for acquisition,positive and negative ion mode switching for simultaneous determination,and external standard method for quantification.[Results]In the linear ranges of the target substances,the correlation coefficients R^(2)were all greater than 0.99.The detection limits of the method was in the range of 0.001-0.05μg/kg,and the quantitation limits were in the range of 0.03-0.20 ng/L.The recovery values ranged from 72.8%to 110.5%.[Conclusions]This method has high sensitivity and good accuracy,and thus strong practical value.
文摘On the stone-paved lanes of Songyang County that date back to ancient times,morning mist lingered as a faint fragrance of tea wafted from a century-old house.Inside,Yang Junjie,a tea maker born in the 1980s,worked deftly at the stove,his hands moving swiftly over the scorching iron wok as tender green tea leaves dance between his fingers.
基金supported by Project of Zhangjiajie City’s Industry-Academia-Research Collaborative Innovation‘Open Project Leader’,Zhangjiajie City’s Project of Taking on Challenging Tasks by Responding to Calls for Solutions(JB20230530)Basic Research Funds Project for Central Universities of China(2023XCZX001,2020kfyXJJS120)Academician and Expert Workstations in Yunnan Province(202205AF150090).
文摘Vine tea is documented in ancient Chinese books as having the function of promoting blood circulation.However,its effects and mechanisms remain unclear.The aim of this study was to comprehensively investigate the protective potential and mechanisms of vine tea in high-fat diet rat through a combination of in vivo and in vitro experiments.The efficacy of vine tea was evaluated using a high-fat diet rat model and an oxidized low-density lipoprotein-treated cell model,with physiological and biochemical indicators measured in rat serum and cell supernatants.Transcriptomics was utilized to investigate alterations mRNA expression following the administration of dihydromyricetin in cell model.Metabolomics and 16S rRNA sequencing was employed to examine changes in metabolites in the serum and changes in gut microbiota of high-fat diet rats after administering vine tea extract.Vine tea extract and dihydromyricetin can reduce elevated levels of lipids,including total cholesterol and triglycerides,following modeling.Transcriptomic data indicate that dihydromyricetin exerts its effects by regulating ferroptosis signaling pathways.Metabolomic analysis demonstrates that the administration of vine tea extract influences the vitamin K cycle and glutathione,thereby alleviating the progression of ferroptosis.Additionally,16S rRNA sequencing reveals that vine tea extract increases Lactobacillaceae in the gut microbiota,which subsequently affects the levels of lysophosphatidylcholine,a major phospholipid component of oxidized low-density lipoprotein in serum.Our results indicate that vine tea can regulate ferroptosis signaling pathways and increase Lactobacillaceae in the gut microbiota,thereby exhibiting cardiovascular protective effects in high-fat diet rats.
基金supported by the National Key Research and Development Program of China(2022YFD2101101)the Earmarked Fund for CARS-19+2 种基金the National Natural Science Foundation of China(32402634)the Modern Agricultural(Tea)Industry Technology System of Fujian Province,China(2025 No.593)the Special Fund for Science and Technology Innovation of Fujian Zhang Tianfu Tea Development Foundation,China(FJZTF01)。
文摘Understanding the molecular responses of tea leaves to mechanical stress is crucial for elucidating the mechanisms of post-harvest quality formation during oolong tea processing.This study employed an integrated multi-omics strategy to characterize the changes and interactions among metabolomic(MB),transcriptomic(TX),and proteomic(PT)profiles in mechanically stressed tea leaves.Mechanical stress initially activated damage-associated molecular patterns(DAMPs),including Ca^(2+)signaling,jasmonic acid signaling,and glutathione metabolism pathways.These processes subsequently induced quality-related metabolic pathways(QRMPs),particularly α-linolenic acid and phenylalanine metabolism.Upregulated expression of LOX,ADH1,and PAR genes,together with the increased abundance of their encoded proteins,respectively promoted the accumulation of jasmine lactone,benzyl alcohol,and 2-phenylethanol.These findings indicate that mechanical stress influences the metabolite biosynthesis in tea leaves through coordinated molecular responses.This study provides new insights into the molecular mechanisms underlying tea leaf responses to mechanical stress and a foundation for future investigations into how early molecular events may contribute to post-harvest metabolic changes during oolong tea processing.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072630,32372774,and U22A20499)the earmarked fund for CARS(Grant No.CARS-19-01A).
文摘Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
文摘May 21,2024 marks the 5th.International Tea Day.On May 23,the Chinese Embassy in the UK held the 2024 Tea for Harmony Yaji Cultural Salon.At the event,vips were treated with three Hubei speciality teas,namely Enshi Yulu green tea,Yihong black tea,and Green Brick team,introduced to the history and culture of Chinese tea.
基金"Study on the Active Components and Healthcare Function of Pu-erh Tea"(2007BAD58B04-2)"National Tea Industrial Science and Technology System"from Modern Agriculture Industrial Technology System,Yunnan Agricultural University
文摘A simple and accurate high-performance liquid chromatography（HPLC）method to generate the fingerprints of crude Pu-erh tea（CPT）and ripened Pu-erh tea（RPT）is described.A suitable chromatographic system was established using a linear gradient elution with acetonitrile and water containing 0.6% formic acid as the mobile phase and a detection wavelength of 300 nm.HPLC analysis identified 24 common peaks among RPT and 21 common peaks among CPT.This study revealed that crude Pu-erh tea and ripened Pu-erh tea contained some similar major constituents,but with distinctive peaks,which may be caused by the difference in the fermentation process.This HPLC fingerprint method could be used to evaluate and authenticate crude Pu-erh tea and ripened Pu-erh tea.
基金Supported by Project from Anhui Academy of Agricultural Sciences(16A1133)
文摘[Objective] This study was conducted to select a batch of new high-effi- cient low-toxicity high-selectivity pesticides for the application in tea gardens. [Method] This experiment was designed with 12 treatments including clear water as the CK, in randomized block arrangement, with 4 replications, and each plot had an area of 30 m2. The pesticides were sprayed in the high occurrence period of larvae of tea lesser leafhopper when tea shoots grew vigorously. [Result] In the 11 pesti- cides, 100 g/L bifenthrin EC , 70% imidacloprid WDG and 150 g/L indoxacarb EC were the best pesticides with efficacies above 90%; and in the bio-pesticides, 0.5% azadirachtin SC showed an efficacy over 87.97%. [Conclusions] Among the tested pesticides, 100 g/L bifenthrin EC , 70% imidacloprid WDG and 150 g/L indoxacarb EC could effectively control tea lesser leafhopper during the outbreak of tea lesser leafhopper, and when the occurrence degree of tea lesser leafhopper is below the medium level, environment-friendly 0.5% azadirachtin SC, 0.5% veratrine SC, 0.3% matrine SC and 4.0×10^10 spores/g Beauveria bassiana OD could be selected.
