Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domest...Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domestic ungulates,commonly used for food and as bioreactors.Generating stable pluripotent porcine PSC lines remains challenging.So far,the pluripotency gene network of porcine PSCs is poorly understood.Here we found that TBX3-derived induced pluripotent stem cells(iPSCs)closely resemble porcine 4-cell embryos with the capacity of totipotent-like stem cells(TLSCs).Interestingly,our data suggest that TBX3 facilitates the activation of H3K4me3 methyltransferase,specifically MLL1.Subsequent investigations revealed that the porcine 4-cell specific gene,MCL1,is a key downstream effector of the TBX3-MLL1 axis.Together,our study of the TBX3 regulatory network is helpful in the understanding of the totipotency characteristics of pigs.展开更多
目的通过检测常染色体显性遗传性多囊肾(ADPKD)患者肾组织中TBX1基因的表达,探讨其在多囊肾发病中的分子遗传学机制。方法 ADPKD多囊肾肾组织11例为病例组;正常肾组织7例为对照组。实时定量聚合酶链反应(RT-PCR)法检测肾组织TBX1 m RNA...目的通过检测常染色体显性遗传性多囊肾(ADPKD)患者肾组织中TBX1基因的表达,探讨其在多囊肾发病中的分子遗传学机制。方法 ADPKD多囊肾肾组织11例为病例组;正常肾组织7例为对照组。实时定量聚合酶链反应(RT-PCR)法检测肾组织TBX1 m RNA表达;Western blot及免疫组化法检测TBX1蛋白表达。采用SPSS 13.0软件比较两组差异。结果ADPKD多囊肾肾组织TBX1 m RNA及蛋白表达较对照组均明显增强,差异有统计学意义(P<0.01);TBX1在对照组肾组织的肾小管上皮细胞中有微量表达,而在多囊肾囊肿组织上皮细胞中有较强的表达。结论在ADPKD发病过程中TBX1基因过度表达可能是ADPKD发生的一种潜在致病机制。展开更多
基金funded by the National Basic Research Program of China(2022YFD1302200,2023YFF1000904,2023ZD0404303 and 2021YFD1200301)the National Natural Science Foundation of China(32072806,32372970 and 32002246)+2 种基金the Program of Shaanxi Province Science and Technology Innovation Team,China(2019TD-036)the Key Technologies Demonstration of Animal Husbandry in Shaanxi Province,China(20221086 and 20230978)the Inner Mongolia Autonomous Region Open Competition Project,China(2022JBGS0025).
文摘Pluripotent stem cells(PSCs)are useful for developmental and translational research because they have the potential to differentiate into all cell types of an adult individual.Pigs are one of the most important domestic ungulates,commonly used for food and as bioreactors.Generating stable pluripotent porcine PSC lines remains challenging.So far,the pluripotency gene network of porcine PSCs is poorly understood.Here we found that TBX3-derived induced pluripotent stem cells(iPSCs)closely resemble porcine 4-cell embryos with the capacity of totipotent-like stem cells(TLSCs).Interestingly,our data suggest that TBX3 facilitates the activation of H3K4me3 methyltransferase,specifically MLL1.Subsequent investigations revealed that the porcine 4-cell specific gene,MCL1,is a key downstream effector of the TBX3-MLL1 axis.Together,our study of the TBX3 regulatory network is helpful in the understanding of the totipotency characteristics of pigs.
文摘目的通过检测常染色体显性遗传性多囊肾(ADPKD)患者肾组织中TBX1基因的表达,探讨其在多囊肾发病中的分子遗传学机制。方法 ADPKD多囊肾肾组织11例为病例组;正常肾组织7例为对照组。实时定量聚合酶链反应(RT-PCR)法检测肾组织TBX1 m RNA表达;Western blot及免疫组化法检测TBX1蛋白表达。采用SPSS 13.0软件比较两组差异。结果ADPKD多囊肾肾组织TBX1 m RNA及蛋白表达较对照组均明显增强,差异有统计学意义(P<0.01);TBX1在对照组肾组织的肾小管上皮细胞中有微量表达,而在多囊肾囊肿组织上皮细胞中有较强的表达。结论在ADPKD发病过程中TBX1基因过度表达可能是ADPKD发生的一种潜在致病机制。
