Ganoderma is a well-known type of medicine food homology fungal taxa and is frequently utilized as a dietary supplement in traditional healthcare practices across Asian countries.With the development of molecular biol...Ganoderma is a well-known type of medicine food homology fungal taxa and is frequently utilized as a dietary supplement in traditional healthcare practices across Asian countries.With the development of molecular biology,an increasing number of Ganoderma species have been sorted out,making it necessary to further clarify the chemical composition of these species.The extracts collected from thirteen Ganoderma species were analyzed using LC-MS/MS and their metabolites were organized into a molecular network mapped with taxonomic information,which was used to determine the priority species.The subsequent MS-guided isolation of the solid culture of Ganoderma shandongense led to the discovery of 11 new triterpenoids(1-11)and 2 known ones(12–13).Their structures were confirmed by HRESIMS and NMR spectra.The absolute configurations were established by NMR calculations,modified Mosher’s method,and Mo_(2)(OAc)4-induced ECD analyses.The inhibitory effects of all compounds on AChE were assessed,and compounds 1,6,and 12 demonstrated better performance than the positive control,with IC_(50) values of 24.91,31.52,and 16.39μM,respectively.In addition,kinetic studies indicated that both 1 and 6 operate as mixed-type inhibitors,and the CD spectra revealed that 1 and 6 induced alterations in the secondary structure of AChE.Molecular docking suggested that 1 and 6 bind to the peripheral anionic site to inhibit AChE catalytic activity.展开更多
基金the National Natural Science Foundation of China(Project No.32100013 and 82104060)the Science and Technology Planning Project of Liaoning Province(2023-BSBA-328)the Fund of CAS Key Laboratory of Forest Ecology and Silviculture,Institute of Applied Ecology,Chinese Academy of Sciences(No.KLFES-2028).
文摘Ganoderma is a well-known type of medicine food homology fungal taxa and is frequently utilized as a dietary supplement in traditional healthcare practices across Asian countries.With the development of molecular biology,an increasing number of Ganoderma species have been sorted out,making it necessary to further clarify the chemical composition of these species.The extracts collected from thirteen Ganoderma species were analyzed using LC-MS/MS and their metabolites were organized into a molecular network mapped with taxonomic information,which was used to determine the priority species.The subsequent MS-guided isolation of the solid culture of Ganoderma shandongense led to the discovery of 11 new triterpenoids(1-11)and 2 known ones(12–13).Their structures were confirmed by HRESIMS and NMR spectra.The absolute configurations were established by NMR calculations,modified Mosher’s method,and Mo_(2)(OAc)4-induced ECD analyses.The inhibitory effects of all compounds on AChE were assessed,and compounds 1,6,and 12 demonstrated better performance than the positive control,with IC_(50) values of 24.91,31.52,and 16.39μM,respectively.In addition,kinetic studies indicated that both 1 and 6 operate as mixed-type inhibitors,and the CD spectra revealed that 1 and 6 induced alterations in the secondary structure of AChE.Molecular docking suggested that 1 and 6 bind to the peripheral anionic site to inhibit AChE catalytic activity.
