Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent ...Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent magnetic nanoparticles(FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human i PS cells were prepared and cultured for 72 h. The culture medium was collected, and then was coincubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human i PS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results: iP S cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iP S cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion: FMNP-labeled human i PS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.展开更多
Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran ...Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran and poly(ethyleneimine) for functionalization with folic acid and cyanine 7. The functionalized BP nanoparticles exhibit excellent biocompatibility, stability, and near infrared optical properties for targeted imaging of tumors through photoacoustic imaging and near-infrared fluorescence imaging.They also display high photothermal conversion efficiency for photothermal therapy of cancer. This work demonstrates the potential of functionalized small BP nanoparticles as an emerging nanotheranostic agent for the diagnosis and treatment of cancer.展开更多
Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases...Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases.Impact Statement:This study develops a new technology that can accurately visualize the thrombus and deliver drugs with controllable properties to diagnose and treat thrombotic diseases.Introduction:Thrombotic diseases are a serious threat to human life and health.The diagnosis and treatment of thrombotic diseases have always been a challenge.In recent years,nanomedicine has brought new ideas and new methods for the theranostics of thrombotic diseases.However,there are also many problems need to be solved,such as biosafety and stability of nanocarriers,early diagnosis,and timely treatment of thrombotic diseases,difficulty in clinical translation.Methods:The S1P@CD-PLGA-rtPA nanobubbles(NBs)were prepared by integrating sulfur hexafluoride(SF6)-loaded poly(D,L-lactide-co-glycolide)(PLGA)NBs,cyclodextrin(CD),sphingosine-1-phosphate(S1P),and recombinant tissue plasminogen activator(rtPA).Results:S1P@CD-PLGA-rtPA NBs had rapid and excellent thrombosis targeting imaging performance based on the specific interaction of S1P-S1PR1(sphingosine-1-phosphate receptor 1).Furthermore,S1P@CD-PLGA-rtPA NBs that specifically targeting to the thrombosis regions could also respond to external ultrasound to achieve accurate and efficient delivery of rtPA to enhance the thrombolysis effectiveness and efficiency.Conclusion:This study proposes a new idea and strategy of targeting thrombus in rats via the specific interaction of S1P-S1PR1.On this basis,the acoustic response properties of bubble carriers could be fully utilized by combining thrombus-specific targeted imaging and ultrasound-mediated drug delivery for effective thrombolysis,which is expected to be applied in targeted diagnosis and treatment of thrombotic diseases in the future.展开更多
Space target imaging simulation technology is an important tool for space target detection and identification,with advantages that include high flexibility and low cost.However,existing space target imaging simulation...Space target imaging simulation technology is an important tool for space target detection and identification,with advantages that include high flexibility and low cost.However,existing space target imaging simulation technologies are mostly based on target magnitudes for simulations,making it difficult to meet image simulation requirements for different signal-to-noise ratio(SNR)needs.Therefore,design of a simulation method that generates target image sequences with various SNRs based on the optical detection system parameters will be important for faint space target detection research.Addressing the SNR calculation issue in optical observation systems,this paper proposes a ground-based detection image SNR calculation method using the optical system parameters.This method calculates the SNR of an observed image precisely using radiative transfer theory,the optical system parameters,and the observation environment parameters.An SNR-based target sequence image simulation method for ground-based detection scenarios is proposed.This method calculates the imaging SNR using the optical system parameters and establishes a model for conversion between the target’s apparent magnitude and image grayscale values,thereby enabling generation of target sequence simulation images with corresponding SNRs for different system parameters.Experiments show that the SNR obtained using this calculation method has an average calculation error of<1 dB when compared with the theoretical SNR of the actual optical system.Additionally,the simulation images generated by the imaging simulation method show high consistency with real images,which meets the requirements of faint space target detection algorithm research and provides reliable data support for development of related technologies.展开更多
Aim To propose a generalized and closed representation of the Wigner Ville Hough transform(WVHT), for the moving target detection and imaging in the design of synthetic aperture radar(SAR). Methods Based on the li...Aim To propose a generalized and closed representation of the Wigner Ville Hough transform(WVHT), for the moving target detection and imaging in the design of synthetic aperture radar(SAR). Methods Based on the line integral, the WVH transform was derived by combining the Wigner Ville distribution (WVD) and the Hough transform (HT) together. The new transform was then verified with computer by the simulated SAR echoes. Results and Conclusion The correctness and the validity of the WVH transform were proved by the computer simulation. Compared with the conventional WVD HT method, the new approach based on the WVHT can simplify the processing procedure, it can translate the chirp echoes of multi targets of SAR from the time domain into the parameter space directly, while suppressing the cross terms of WVD and estimating the motion coefficients for the final imaging. It is obvious that the WVH transform can be also used in other cases for the chirp signal detection.展开更多
In recent years,multicolor cascade supramolecular assemblies with controllable topological morphology have become a research hotspot due to their wide application in light-emitting materials,cell imaging and other fie...In recent years,multicolor cascade supramolecular assemblies with controllable topological morphology have become a research hotspot due to their wide application in light-emitting materials,cell imaging and other fields.Herein,several kinds of macrocycles including cucurbiturils,calixarene and cyclodextrins are used as building blocks to construct fluorescent assemblies with anthryl-conjugated phenylpyridine(G),wherein cucurbit[8]uril(CB[8])and G can form nanowires at a stoichiometric ratio of n:n through host-vip encapsulation to form a non-covalent heterodimer.Significantly,the macrocycle confinement effect drastically enhances the fluorescence emission of G and emission peak generated bathochromic shift from 500 nm to 600 nm.When the supramolecular polymer is further assembled with amphiphilic calix[4]arene(SC_(4)A8),the fluorescence emission of G?CB[8]further increases to 1.4 times,accompanied by the morphological transformation from linear structure to nanorod structure.Subsequently,a very small amount of dye Cy5 is added to the assembly solution as an energy receptor,and the negatively charged G?CB[8]@SC_(4)A8 system is regarded as an energy donor.The efficient energy transfer process enables near-infrared(NIR)emission at 675 nm with 71%energy transfer efficiency(ΦET)at a donor/receptor ratio of 100:1.Finally,the cascade supramolecular assembly has been successfully applied to targeted imaging in the nucleus of HeLa and A549 cancer cells.展开更多
Based on the measurement model of inverse synthetic aperture radar (ISAR) within a small aspect sector,an imaging method was presented with the application of sparse signal processing.This method can form higher resol...Based on the measurement model of inverse synthetic aperture radar (ISAR) within a small aspect sector,an imaging method was presented with the application of sparse signal processing.This method can form higher resolution inverse synthetic aperture radar images from compensating incomplete measured data,and improves the clarity of the images and makes the feature structure much more clear,which is helpful for target recognition.The simulation results indicate that this method can provide clear ISAR images with high contrast under complex motion case.展开更多
Compared with the side-looking Synthetic Aperture Radar (SAR), the flexible beampointing of squint SAR makes great application value. This paper derives the image signature of the ground moving target after the proces...Compared with the side-looking Synthetic Aperture Radar (SAR), the flexible beampointing of squint SAR makes great application value. This paper derives the image signature of the ground moving target after the processing of Range-Doppler (RD) algorithm, the SAR signatures of ground moving targets are analyzed, including the geometry correction, the offsets and defocusing in both range and azimuth direction. Finally, computer simulation results validate its effectiveness. The research results are especially significant for moving targets detection and parameters estimation in squint mode SAR.展开更多
Based on dual-frequencies dual-apertures spaceborne SAR （Synthetic Aperture Radar）, a new SAR system with four receiving channels and two operation modes is presented in this paper, SAR imaging and Moving Target Ind...Based on dual-frequencies dual-apertures spaceborne SAR （Synthetic Aperture Radar）, a new SAR system with four receiving channels and two operation modes is presented in this paper, SAR imaging and Moving Target Indication （MTI） are studied in this system. High resolution imaging with wide swath is implemented by the Mode Ⅰ, and MTI is completed by the Mode Ⅱ. High azimuth resolution is achieved by the Displaced Phase Center （DPC） multibeam technique. And the Coherent Accumulation （CA） method, which combines dual channels data of different carrier frequency, is used to enhance the range resolution. For the data of different carrier frequency, the two aperture interferometric processing is executed to implement clutter cancellation, respectively. And the couple of clutter suppressed data are employed to implement Dual Carrier Frequency Conjugate Processing （DCFCP）, then both slow and fast moving targets detection can be completed, followed by moving target imaging. The simulation results show the validity of the signal processing method of this new SAR system.展开更多
The optimal imaging time selection of ship targets for shore-based inverse synthetic aperture radar （ISAR） in high sea conditions is investigated. The optimal imaging time includes opti- mal imaging instants and opt...The optimal imaging time selection of ship targets for shore-based inverse synthetic aperture radar （ISAR） in high sea conditions is investigated. The optimal imaging time includes opti- mal imaging instants and optimal imaging duration. A novel method for optimal imaging instants selection based on the estimation of the Doppler centroid frequencies （DCFs） of a series of images obtained over continuous short durations is proposed. Combined with the optimal imaging duration selection scheme using the image contrast maximization criteria, this method can provide the ship images with the highest focus. Simulated and real data pro- cessing results verify the effectiveness of the proposed imaging method.展开更多
This paper proposes a novel inverse synthetic aperture radar（ISAR） imaging method based on second-order keystone transform（KT） and Sandglass transform for group targets flying in a formation with constant accelera...This paper proposes a novel inverse synthetic aperture radar（ISAR） imaging method based on second-order keystone transform（KT） and Sandglass transform for group targets flying in a formation with constant accelerated rectilinear motion in the same radar beam. First, range curvature and range walk of each sub-target among group targets are corrected by the second-order KT combined with the quadratic phase term compensation. After range alignment, the signals in each range frequency cell can be modelled as multiple chirp signals and then the Sandglass transform is utilized to cross-range imaging, which transforms the time–frequency distribution of the signals in each range frequency cell into beelines parallel to the slow time axis simultaneously. Finally, cross-range profiles of group targets in each range frequency cell are obtained via a projection of the perk of every scatterer in the two-dimensional accumulation plane onto the frequency axis. The advantage of the proposed method is that it can align range profiles of each sub-target simultaneously and image cross-range profiles directly without separating the returned signals, which simplifies the operation procedure. Simulation results are used to demonstrate the effectiveness of the proposed method.展开更多
How to enhance the therapeutic efficacy of human cytokine induced killers cell(CIK)has become a great challenge.Herein,we report for the first time that human CIK cells loaded with gold nanoprisms were successfully us...How to enhance the therapeutic efficacy of human cytokine induced killers cell(CIK)has become a great challenge.Herein,we report for the first time that human CIK cells loaded with gold nanoprisms were successfully used for targeted photoacoustic imaging,enhanced immunotherapy and photothermal therapy of gastric cancer in vivo.Gold nanoprisms were synthesized and modified with PEG;human CIK cells were prepared and incubated with PEGylated gold nanoprisms(Au GNPrs),and then the effects of human CIK cells labeled with Au NPrs on gastric cancer MGC 803 cells were evaluated and further used for targeted photoacoustic imaging,immunotherapy and photothermal therapy of gastric cancer in vivo in mice models.Results showed that PEGylated Au NPrs could be uptaken high-efficiently by human CIK cells,resultant human CIK cells labeled with AuNPrs could inhibit the growth of gastric cancer MGC 803 cells actively by induced apoptosis and G1 phase arrest,and actively target and accumulate the tumor sites in gastric cancer-bearing nude mice.Enhanced synergistic therapeutic efficacy was demonstrated with the maximal inhibition of tumor through a combination of CIK cells-based immunotherapy for three days and then a continuous gold nanoprisms-based photothermal therapy.In conclusion,human CIK cells labeled with PEGylated Au NPrs can target gastric cancer cells in vivo,enhance immunotherapy and photothermal therapy efficacy,and have a great potential in applications such as targeted imaging,immunotherapy and photothermal therapy of gastric cancer in the near future.展开更多
In this paper, near-infrared emitting long-persistence luminescent Zn3Ga2Ge2O10:Cr3?(ZGG) nanoparticles with diameters of 30–100 nm and bright luminescence were prepared by a sol–gel synthesis method. After the surf...In this paper, near-infrared emitting long-persistence luminescent Zn3Ga2Ge2O10:Cr3?(ZGG) nanoparticles with diameters of 30–100 nm and bright luminescence were prepared by a sol–gel synthesis method. After the surface amination, the nanoparticles were further bioconjugated with breast cancer-specific monoclonal antibody(anti-Ep CAM) to form ZGG-Ep CAM nanoprobes which can specifically target breast cancer cell lines(MCF7) in vitro. The results of in vitro images show that the luminescence signals from the cells treated with ZGG-Ep CAM nanoprobes are stronger than those from cells treated with ZGG-unconjugated antibody, indicating that the prepared ZGG-Ep CAM nanoprobes possessed excellent specific recognition capability. Furthermore, due to their long afterglow properties, the imaging could persist more than 1 h. Therefore, these nanoprobes could not only provide a high specificity detection method for cancer cells but also realize the long-time monitoring. Developed near-infrared emitting long-persistence luminescent nanoprobes will be expected to find new perspectives for cell therapy research and diagnosis applications.展开更多
In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes...In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes made them of no use in real-time applications.Inspired by this,we propose another efficient and rapid image encryption plan dependent on the Trigonometric chaotic guide.In contrast to the most of current plans,we utilize this basic map to create just a couple of arbitrary rows and columns.Moreover,to additionally speed up,we raise the processing unit from the pixel level to the row/column level.The security of the new plot is accomplished through a substitution permutation network,where we apply a circular shift of rows and columns to break the solid connection of neighboring pixels.At that point,we join the XOR operation with modulo function to cover the pixels values and forestall any leaking of data.High-security tests and simulation analyses are carried out to exhibit that the scheme is very secure and exceptionally quick for real-time image processing at 80 fps(frames per second).展开更多
Background Magnetic resonance （MR） molecular imaging can detect abnormalities associated with disease at the level of cell and molecule. The epidermal growth factor receptor （EGFR） plays an important role in the d...Background Magnetic resonance （MR） molecular imaging can detect abnormalities associated with disease at the level of cell and molecule. The epidermal growth factor receptor （EGFR） plays an important role in the development of lung cancer. This study aimed to explore new MR molecular imaging targeting of the EGFR on lung cancer cells. Methods We attached ultra-small superparamagnetic iron oxide （USPIO） particles to cetuximab （C225） anti-human IgG using the carbodiimide method. We made the molecular MR contrast agents C225-USPIO and IgG-USPIO, the latter as a control reagent, and determined concentrations according to the Fe content. Lung cancer A549 cells were cultured and immunocytochemistry （SP） was used to detect the expression of EGFR on cells. We detected the binding rate of C225-USPIO to A549 cells with immunofluorescence staining and flow cytometry. We cultured A549 cells with C225-USPIO at a Fe concentration of 50 pg/ml and assayed the binding of C225-USPIO after 1 hour with Prussian blue staining and transmission electron microscopy （TEM）. We determined the effects on imaging of the contrast agent targeted to cells using a 4.7T MRI. We did scanning on the cells labeled with C225-USPIO, IgG-USPIO, and distilled water, respectively. The scanning sequences included axial T1WI, T2WI. Results Immunocytochemical detection of lung cancer A549 cells found them positive for EGFR expression. Immunofluorescence staining and flow cytometry after cultivation with different concentrations of C225-USPIO showed the binding rate higher than the control. Prussian blue staining and transmission electron microscopy revealed that in the C225-USPIO contrast agent group of cells the particle content of Fe in cytoplasmic vesicles or on surface was more than that in the control group. The 4.7T MR imaging （MRI） scan revealed the T2WI signal in the C225-USPIO group of cells decreased significantly more than in unlabeled cells, but there was no significant difference between the time gradients. Conclusions We successfully constructed the molecular imaging agent C225-USPIO targeting the EGFR of A549 lung cancer cells. The imaging agent showed good targeting effect and specificity, and reduced MRI T2 value significantly, thus such molecular contrast agents could provide a new way to measure EGFR levels.展开更多
Targeted-delivery is of great importance to molecular probes and drugs for cell biology study. Herein we reported 11 sulfur-containing coumarins as cell imaging probes. Different sulfur speciation of the 4 representat...Targeted-delivery is of great importance to molecular probes and drugs for cell biology study. Herein we reported 11 sulfur-containing coumarins as cell imaging probes. Different sulfur speciation of the 4 representative coumarins SC1-SC4 renders them significantly different subcellular localizations and cellular uptake pathways： SC1 containing thioether group located in lysosomes, while sulfoxide and sulfone compounds SC2 and SC3 distributed in the whole cell. Furthermore, the cationic sulfonium containing compound SC4 was internalized by clathrin-mediated endocytosis and localized at mitochondria. By analyzing the molecular parameters of all 11 coumarins, we found that different sulfur speciation affected their lipophilicity and electrostatic surface potential. These two key factors play roles in altering biological behaviors of the coumarins. The results revealed the importance of sulfur speciation on the physicochemical properties and thus subcellular localization of bioprobes. This is useful for designing new functional bioprobes.展开更多
The parametric scattering center model of radar tar-get has the advantages of simplicity,sparsity and mechanism relevant,making it widely applied in fields such as radar data compression and rapid generation,radar ima...The parametric scattering center model of radar tar-get has the advantages of simplicity,sparsity and mechanism relevant,making it widely applied in fields such as radar data compression and rapid generation,radar imaging,feature extraction and recognition.This paper summarizes and analyzes the research situation,development trend,and difficult prob-lems on scattering center(SC)parametric modeling from three aspects:parametric representation,determination method of model parameters,and application.展开更多
This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the sha...This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the shapes are segregated into various regions according to its nature;otherwise, it is treated as textured image and considered the entire image as it is for the experiment. The aforesaid tests are applied regions-wise. First, the F-ratio test is applied, if the images pass the test, then it is proceeded to test the spectrum of energy, i.e. means of the two images. If the images pass both tests, then it is concluded that the two images are the same or similar. Otherwise, they differ. Since the proposed system is distribution-based, it is invariant for rotation and scaling. Also, the system facilitates the user to fix the number of images to be retrieved, because the user can fix the level of significance according to their requirements. These are the main advantages of the proposed system.展开更多
To achieve accurate classification and recognition of ship target types,it is necessary to establish a sample library of ship targets to be identified.On the basis of exploring the principles of building a ship target...To achieve accurate classification and recognition of ship target types,it is necessary to establish a sample library of ship targets to be identified.On the basis of exploring the principles of building a ship target image library,the paper determines the sample set.Using 3DS MAX software as the platform,combined with the accurate 3D model of the ship in an offline state,the software fully utilizes its own rendering and animation functions to achieve the automatic generation of multi-view and multi-scale views of ship targets.To reduce the storage capacity of the image database,a construction method of the ship target image database based on the AP algorithm is presented.The algorithm can obtain the optimal cluster number,reduce the data storage capacity of the image database,and save the calculation amount for the subsequent matching calculation.展开更多
基金supported by National Natural Science Foundation of China (Grant No. 81225010, 20803040, 81028009, and 31170961)National Key Basic Research Program of China (973 Program) (Grant No. 2010CB933902 and 2015CB931802)+1 种基金National Key Technology Research and Development Program (863 Program) (Grant No. 2012AA022703 and 2014AA020700)Shanghai Science and Technology Fund (Grant No.13NM1401500)
文摘Objective: Human induced pluripotent stem(i PS) cells exhibit great potential for generating functional human cells for medical therapies. In this paper, we report for use of human i PS cells labeled with fluorescent magnetic nanoparticles(FMNPs) for targeted imaging and synergistic therapy of gastric cancer cells in vivo. Methods: Human i PS cells were prepared and cultured for 72 h. The culture medium was collected, and then was coincubated with MGC803 cells. Cell viability was analyzed by the MTT method. FMNP-labeled human i PS cells were prepared and injected into gastric cancer-bearing nude mice. The mouse model was observed using a small-animal imaging system. The nude mice were irradiated under an external alternating magnetic field and evaluated using an infrared thermal mapping instrument. Tumor sizes were measured weekly. Results: iP S cells and the collected culture medium inhibited the growth of MGC803 cells. FMNP-labeled human iP S cells targeted and imaged gastric cancer cells in vivo, as well as inhibited cancer growth in vivo through the external magnetic field. Conclusion: FMNP-labeled human i PS cells exhibit considerable potential in applications such as targeted dual-mode imaging and synergistic therapy for early gastric cancer.
基金support from the National Natural Science Foundation of China(81471657,81527901)the 1000 Plan for Young Talents,Jiangsu Specially Appointed Professorship+2 种基金Jiangsu Provincial Program for Innovative and Entrepreneurial Talentssupport from the Jiangsu Provincial Key Laboratory of Radiation Medicine and Protectionthe Priority Academic Development Program of Jiangsu Higher Education Institutions(PAPD)
文摘Black phosphorus(BP) nanomaterials have attracted extensive attention due to their unique physical,chemical, and biological properties. In this study, small BP nanoparticles were synthesized and modified with dextran and poly(ethyleneimine) for functionalization with folic acid and cyanine 7. The functionalized BP nanoparticles exhibit excellent biocompatibility, stability, and near infrared optical properties for targeted imaging of tumors through photoacoustic imaging and near-infrared fluorescence imaging.They also display high photothermal conversion efficiency for photothermal therapy of cancer. This work demonstrates the potential of functionalized small BP nanoparticles as an emerging nanotheranostic agent for the diagnosis and treatment of cancer.
基金supported by the project of National Natural Science Foundation of China(81971750)Natural Science Foundation of Jiangsu Province(Grant No.BK20222002)+1 种基金National Key Research and Development Program of China(2018YFA0704103)Project 333 of Jiangsu Province.
文摘Objective:The objective of this work is to design and fabricate a novel multifunctional nanocarrier combining thrombus-targeted imaging and ultrasound-mediated drug delivery for the theranostics of thrombotic diseases.Impact Statement:This study develops a new technology that can accurately visualize the thrombus and deliver drugs with controllable properties to diagnose and treat thrombotic diseases.Introduction:Thrombotic diseases are a serious threat to human life and health.The diagnosis and treatment of thrombotic diseases have always been a challenge.In recent years,nanomedicine has brought new ideas and new methods for the theranostics of thrombotic diseases.However,there are also many problems need to be solved,such as biosafety and stability of nanocarriers,early diagnosis,and timely treatment of thrombotic diseases,difficulty in clinical translation.Methods:The S1P@CD-PLGA-rtPA nanobubbles(NBs)were prepared by integrating sulfur hexafluoride(SF6)-loaded poly(D,L-lactide-co-glycolide)(PLGA)NBs,cyclodextrin(CD),sphingosine-1-phosphate(S1P),and recombinant tissue plasminogen activator(rtPA).Results:S1P@CD-PLGA-rtPA NBs had rapid and excellent thrombosis targeting imaging performance based on the specific interaction of S1P-S1PR1(sphingosine-1-phosphate receptor 1).Furthermore,S1P@CD-PLGA-rtPA NBs that specifically targeting to the thrombosis regions could also respond to external ultrasound to achieve accurate and efficient delivery of rtPA to enhance the thrombolysis effectiveness and efficiency.Conclusion:This study proposes a new idea and strategy of targeting thrombus in rats via the specific interaction of S1P-S1PR1.On this basis,the acoustic response properties of bubble carriers could be fully utilized by combining thrombus-specific targeted imaging and ultrasound-mediated drug delivery for effective thrombolysis,which is expected to be applied in targeted diagnosis and treatment of thrombotic diseases in the future.
基金supported by Open Fund of National Key Laboratory of Deep Space Exploration(NKDSEL2024014)by Civil Aerospace Pre-research Project of State Administration of Science,Technology and Industry for National Defence,PRC(D040103).
文摘Space target imaging simulation technology is an important tool for space target detection and identification,with advantages that include high flexibility and low cost.However,existing space target imaging simulation technologies are mostly based on target magnitudes for simulations,making it difficult to meet image simulation requirements for different signal-to-noise ratio(SNR)needs.Therefore,design of a simulation method that generates target image sequences with various SNRs based on the optical detection system parameters will be important for faint space target detection research.Addressing the SNR calculation issue in optical observation systems,this paper proposes a ground-based detection image SNR calculation method using the optical system parameters.This method calculates the SNR of an observed image precisely using radiative transfer theory,the optical system parameters,and the observation environment parameters.An SNR-based target sequence image simulation method for ground-based detection scenarios is proposed.This method calculates the imaging SNR using the optical system parameters and establishes a model for conversion between the target’s apparent magnitude and image grayscale values,thereby enabling generation of target sequence simulation images with corresponding SNRs for different system parameters.Experiments show that the SNR obtained using this calculation method has an average calculation error of<1 dB when compared with the theoretical SNR of the actual optical system.Additionally,the simulation images generated by the imaging simulation method show high consistency with real images,which meets the requirements of faint space target detection algorithm research and provides reliable data support for development of related technologies.
文摘Aim To propose a generalized and closed representation of the Wigner Ville Hough transform(WVHT), for the moving target detection and imaging in the design of synthetic aperture radar(SAR). Methods Based on the line integral, the WVH transform was derived by combining the Wigner Ville distribution (WVD) and the Hough transform (HT) together. The new transform was then verified with computer by the simulated SAR echoes. Results and Conclusion The correctness and the validity of the WVH transform were proved by the computer simulation. Compared with the conventional WVD HT method, the new approach based on the WVHT can simplify the processing procedure, it can translate the chirp echoes of multi targets of SAR from the time domain into the parameter space directly, while suppressing the cross terms of WVD and estimating the motion coefficients for the final imaging. It is obvious that the WVH transform can be also used in other cases for the chirp signal detection.
基金National Natural Science Foundation of China(Nos.22131008 and 21971127)the Fundamental Research Funds for the Central Universitiesthe Haihe Laboratory of Sustainable Chemical Transformations for financial support。
文摘In recent years,multicolor cascade supramolecular assemblies with controllable topological morphology have become a research hotspot due to their wide application in light-emitting materials,cell imaging and other fields.Herein,several kinds of macrocycles including cucurbiturils,calixarene and cyclodextrins are used as building blocks to construct fluorescent assemblies with anthryl-conjugated phenylpyridine(G),wherein cucurbit[8]uril(CB[8])and G can form nanowires at a stoichiometric ratio of n:n through host-vip encapsulation to form a non-covalent heterodimer.Significantly,the macrocycle confinement effect drastically enhances the fluorescence emission of G and emission peak generated bathochromic shift from 500 nm to 600 nm.When the supramolecular polymer is further assembled with amphiphilic calix[4]arene(SC_(4)A8),the fluorescence emission of G?CB[8]further increases to 1.4 times,accompanied by the morphological transformation from linear structure to nanorod structure.Subsequently,a very small amount of dye Cy5 is added to the assembly solution as an energy receptor,and the negatively charged G?CB[8]@SC_(4)A8 system is regarded as an energy donor.The efficient energy transfer process enables near-infrared(NIR)emission at 675 nm with 71%energy transfer efficiency(ΦET)at a donor/receptor ratio of 100:1.Finally,the cascade supramolecular assembly has been successfully applied to targeted imaging in the nucleus of HeLa and A549 cancer cells.
基金Project supported by the National Natural Science Foundation of China
文摘Based on the measurement model of inverse synthetic aperture radar (ISAR) within a small aspect sector,an imaging method was presented with the application of sparse signal processing.This method can form higher resolution inverse synthetic aperture radar images from compensating incomplete measured data,and improves the clarity of the images and makes the feature structure much more clear,which is helpful for target recognition.The simulation results indicate that this method can provide clear ISAR images with high contrast under complex motion case.
基金Supported by the National Natural Science Foundation of China (No. 61102110)Natural Science Foundation of Hebei Province (No. F2010001285, F2012203180)
文摘Compared with the side-looking Synthetic Aperture Radar (SAR), the flexible beampointing of squint SAR makes great application value. This paper derives the image signature of the ground moving target after the processing of Range-Doppler (RD) algorithm, the SAR signatures of ground moving targets are analyzed, including the geometry correction, the offsets and defocusing in both range and azimuth direction. Finally, computer simulation results validate its effectiveness. The research results are especially significant for moving targets detection and parameters estimation in squint mode SAR.
基金Supported by the National Natural Science Foundation of China (NSFC) (No.60772103)China National Key Laboratory of Microwave Imaging Technology Foundation (No.9140C1903050804)
文摘Based on dual-frequencies dual-apertures spaceborne SAR （Synthetic Aperture Radar）, a new SAR system with four receiving channels and two operation modes is presented in this paper, SAR imaging and Moving Target Indication （MTI） are studied in this system. High resolution imaging with wide swath is implemented by the Mode Ⅰ, and MTI is completed by the Mode Ⅱ. High azimuth resolution is achieved by the Displaced Phase Center （DPC） multibeam technique. And the Coherent Accumulation （CA） method, which combines dual channels data of different carrier frequency, is used to enhance the range resolution. For the data of different carrier frequency, the two aperture interferometric processing is executed to implement clutter cancellation, respectively. And the couple of clutter suppressed data are employed to implement Dual Carrier Frequency Conjugate Processing （DCFCP）, then both slow and fast moving targets detection can be completed, followed by moving target imaging. The simulation results show the validity of the signal processing method of this new SAR system.
基金supported by the Innovation Foundation for Scientific Research Base(NJ20140008NJ20150018)+1 种基金the Aeronautical Science Foundation of China(20132052035)the National Defense Basic Scientific Research(B2520110008)
文摘The optimal imaging time selection of ship targets for shore-based inverse synthetic aperture radar （ISAR） in high sea conditions is investigated. The optimal imaging time includes opti- mal imaging instants and optimal imaging duration. A novel method for optimal imaging instants selection based on the estimation of the Doppler centroid frequencies （DCFs） of a series of images obtained over continuous short durations is proposed. Combined with the optimal imaging duration selection scheme using the image contrast maximization criteria, this method can provide the ship images with the highest focus. Simulated and real data pro- cessing results verify the effectiveness of the proposed imaging method.
基金supported by the National Natural Science Foundation of China (No. 61372159)
文摘This paper proposes a novel inverse synthetic aperture radar（ISAR） imaging method based on second-order keystone transform（KT） and Sandglass transform for group targets flying in a formation with constant accelerated rectilinear motion in the same radar beam. First, range curvature and range walk of each sub-target among group targets are corrected by the second-order KT combined with the quadratic phase term compensation. After range alignment, the signals in each range frequency cell can be modelled as multiple chirp signals and then the Sandglass transform is utilized to cross-range imaging, which transforms the time–frequency distribution of the signals in each range frequency cell into beelines parallel to the slow time axis simultaneously. Finally, cross-range profiles of group targets in each range frequency cell are obtained via a projection of the perk of every scatterer in the two-dimensional accumulation plane onto the frequency axis. The advantage of the proposed method is that it can align range profiles of each sub-target simultaneously and image cross-range profiles directly without separating the returned signals, which simplifies the operation procedure. Simulation results are used to demonstrate the effectiveness of the proposed method.
基金supported by Chinese Key Basic Research Program(973 Project)(No.2010CB933901 and 2015CB931802)the National Natural Scientific Foundation of China(Grant No.81225010,81327002,and 31170961)+1 种基金863 project of China(no.2012AA022703 and 2014AA020700)Shanghai Science and Technology Fund(No.13NM1401500)。
文摘How to enhance the therapeutic efficacy of human cytokine induced killers cell(CIK)has become a great challenge.Herein,we report for the first time that human CIK cells loaded with gold nanoprisms were successfully used for targeted photoacoustic imaging,enhanced immunotherapy and photothermal therapy of gastric cancer in vivo.Gold nanoprisms were synthesized and modified with PEG;human CIK cells were prepared and incubated with PEGylated gold nanoprisms(Au GNPrs),and then the effects of human CIK cells labeled with Au NPrs on gastric cancer MGC 803 cells were evaluated and further used for targeted photoacoustic imaging,immunotherapy and photothermal therapy of gastric cancer in vivo in mice models.Results showed that PEGylated Au NPrs could be uptaken high-efficiently by human CIK cells,resultant human CIK cells labeled with AuNPrs could inhibit the growth of gastric cancer MGC 803 cells actively by induced apoptosis and G1 phase arrest,and actively target and accumulate the tumor sites in gastric cancer-bearing nude mice.Enhanced synergistic therapeutic efficacy was demonstrated with the maximal inhibition of tumor through a combination of CIK cells-based immunotherapy for three days and then a continuous gold nanoprisms-based photothermal therapy.In conclusion,human CIK cells labeled with PEGylated Au NPrs can target gastric cancer cells in vivo,enhance immunotherapy and photothermal therapy efficacy,and have a great potential in applications such as targeted imaging,immunotherapy and photothermal therapy of gastric cancer in the near future.
基金supported by the Science and Technology Project in Xiamen(3502Z20132012)the Xiamen Southern Oceanographic Center(14GYY008NF08)+2 种基金the Natural Science Foundation of Fujian Province(2011J01220)the Major Program of Department of Science and Technology(2012Y4009)the Science and Technology Planning Project of Xiamen(3502Z20123036)
文摘In this paper, near-infrared emitting long-persistence luminescent Zn3Ga2Ge2O10:Cr3?(ZGG) nanoparticles with diameters of 30–100 nm and bright luminescence were prepared by a sol–gel synthesis method. After the surface amination, the nanoparticles were further bioconjugated with breast cancer-specific monoclonal antibody(anti-Ep CAM) to form ZGG-Ep CAM nanoprobes which can specifically target breast cancer cell lines(MCF7) in vitro. The results of in vitro images show that the luminescence signals from the cells treated with ZGG-Ep CAM nanoprobes are stronger than those from cells treated with ZGG-unconjugated antibody, indicating that the prepared ZGG-Ep CAM nanoprobes possessed excellent specific recognition capability. Furthermore, due to their long afterglow properties, the imaging could persist more than 1 h. Therefore, these nanoprobes could not only provide a high specificity detection method for cancer cells but also realize the long-time monitoring. Developed near-infrared emitting long-persistence luminescent nanoprobes will be expected to find new perspectives for cell therapy research and diagnosis applications.
基金This research work was partially funded by the Chiang Mai University.
文摘In the most recent decades,a major number of image encryption plans have been proposed.The vast majority of these plans reached a highsecurity level;however,their moderate speeds because of their complicated processes made them of no use in real-time applications.Inspired by this,we propose another efficient and rapid image encryption plan dependent on the Trigonometric chaotic guide.In contrast to the most of current plans,we utilize this basic map to create just a couple of arbitrary rows and columns.Moreover,to additionally speed up,we raise the processing unit from the pixel level to the row/column level.The security of the new plot is accomplished through a substitution permutation network,where we apply a circular shift of rows and columns to break the solid connection of neighboring pixels.At that point,we join the XOR operation with modulo function to cover the pixels values and forestall any leaking of data.High-security tests and simulation analyses are carried out to exhibit that the scheme is very secure and exceptionally quick for real-time image processing at 80 fps(frames per second).
基金This study was supported by a grant from the National Natural Science Foundation of China （No. 30470525）.
文摘Background Magnetic resonance （MR） molecular imaging can detect abnormalities associated with disease at the level of cell and molecule. The epidermal growth factor receptor （EGFR） plays an important role in the development of lung cancer. This study aimed to explore new MR molecular imaging targeting of the EGFR on lung cancer cells. Methods We attached ultra-small superparamagnetic iron oxide （USPIO） particles to cetuximab （C225） anti-human IgG using the carbodiimide method. We made the molecular MR contrast agents C225-USPIO and IgG-USPIO, the latter as a control reagent, and determined concentrations according to the Fe content. Lung cancer A549 cells were cultured and immunocytochemistry （SP） was used to detect the expression of EGFR on cells. We detected the binding rate of C225-USPIO to A549 cells with immunofluorescence staining and flow cytometry. We cultured A549 cells with C225-USPIO at a Fe concentration of 50 pg/ml and assayed the binding of C225-USPIO after 1 hour with Prussian blue staining and transmission electron microscopy （TEM）. We determined the effects on imaging of the contrast agent targeted to cells using a 4.7T MRI. We did scanning on the cells labeled with C225-USPIO, IgG-USPIO, and distilled water, respectively. The scanning sequences included axial T1WI, T2WI. Results Immunocytochemical detection of lung cancer A549 cells found them positive for EGFR expression. Immunofluorescence staining and flow cytometry after cultivation with different concentrations of C225-USPIO showed the binding rate higher than the control. Prussian blue staining and transmission electron microscopy revealed that in the C225-USPIO contrast agent group of cells the particle content of Fe in cytoplasmic vesicles or on surface was more than that in the control group. The 4.7T MR imaging （MRI） scan revealed the T2WI signal in the C225-USPIO group of cells decreased significantly more than in unlabeled cells, but there was no significant difference between the time gradients. Conclusions We successfully constructed the molecular imaging agent C225-USPIO targeting the EGFR of A549 lung cancer cells. The imaging agent showed good targeting effect and specificity, and reduced MRI T2 value significantly, thus such molecular contrast agents could provide a new way to measure EGFR levels.
基金financial support from the National Key Basic Research Support Foundation of China(No. 2015CB856301)the National Natural Scientific Foundation of China (Nos. 21571007, 21271013,21321001)
文摘Targeted-delivery is of great importance to molecular probes and drugs for cell biology study. Herein we reported 11 sulfur-containing coumarins as cell imaging probes. Different sulfur speciation of the 4 representative coumarins SC1-SC4 renders them significantly different subcellular localizations and cellular uptake pathways： SC1 containing thioether group located in lysosomes, while sulfoxide and sulfone compounds SC2 and SC3 distributed in the whole cell. Furthermore, the cationic sulfonium containing compound SC4 was internalized by clathrin-mediated endocytosis and localized at mitochondria. By analyzing the molecular parameters of all 11 coumarins, we found that different sulfur speciation affected their lipophilicity and electrostatic surface potential. These two key factors play roles in altering biological behaviors of the coumarins. The results revealed the importance of sulfur speciation on the physicochemical properties and thus subcellular localization of bioprobes. This is useful for designing new functional bioprobes.
基金supported by the National Natural Science Foundation of China(62231001).
文摘The parametric scattering center model of radar tar-get has the advantages of simplicity,sparsity and mechanism relevant,making it widely applied in fields such as radar data compression and rapid generation,radar imaging,feature extraction and recognition.This paper summarizes and analyzes the research situation,development trend,and difficult prob-lems on scattering center(SC)parametric modeling from three aspects:parametric representation,determination method of model parameters,and application.
文摘This paper proposes a novel method based on statistical tests of hypotheses, such as F-ratio and Welch’s t-tests. The input query image is examined whether it is a textured or structured. If it is structured, the shapes are segregated into various regions according to its nature;otherwise, it is treated as textured image and considered the entire image as it is for the experiment. The aforesaid tests are applied regions-wise. First, the F-ratio test is applied, if the images pass the test, then it is proceeded to test the spectrum of energy, i.e. means of the two images. If the images pass both tests, then it is concluded that the two images are the same or similar. Otherwise, they differ. Since the proposed system is distribution-based, it is invariant for rotation and scaling. Also, the system facilitates the user to fix the number of images to be retrieved, because the user can fix the level of significance according to their requirements. These are the main advantages of the proposed system.
文摘To achieve accurate classification and recognition of ship target types,it is necessary to establish a sample library of ship targets to be identified.On the basis of exploring the principles of building a ship target image library,the paper determines the sample set.Using 3DS MAX software as the platform,combined with the accurate 3D model of the ship in an offline state,the software fully utilizes its own rendering and animation functions to achieve the automatic generation of multi-view and multi-scale views of ship targets.To reduce the storage capacity of the image database,a construction method of the ship target image database based on the AP algorithm is presented.The algorithm can obtain the optimal cluster number,reduce the data storage capacity of the image database,and save the calculation amount for the subsequent matching calculation.
