Background： Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogencsis. The aim of this study was to determine whether C to T base substitution within Taq...Background： Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogencsis. The aim of this study was to determine whether C to T base substitution within Taql Vitamin D receptor （VDR） gene （rs731236） in exon 9 was a risk factor among patients with breast cancer. Methods： Peripheral blood was drawn from 122 Jordanian breast cancer patients and 100 healthy Jordanian volunteers in Al-Bashecr Hospital during the summer months （from June to November of 2013, 2014, and 2015）. DNA was amplified using polymerase chain reaction （PCR）, followed by Taql restriction enzyme digestion. Quantification of serum 25-hydroxy Vitamin D （25[OH]D） level was determined by competitive immunoassay Elecsys. Results： Genotypic frequencies for Taql TT, Tt, and tt genotypes were 41%, 46%, and 13% for breast cancer compared to 42%, 50%. and 8% for control, respectively. Vitamin D serum level was significantly lower in the breast cancer patients （8.1 ± 0.3 ng/ml） compared to the control group （21.2± 0.6 ng/ml; P =0.001）. This study showed an inverse association between 25（OH）D serum level and brcasl cancer risk （odds ratio [OR], 22.72, 95% confidence interval [C/], 10.06 51.29）. Conclusions： An inverse association was found between 25（OH）D serum level and breast cancer risk. Statistical difference was also found between different VDR Taql genotypes and circulating levels of 25（OH）D among Jordanian females with breast cancer.展开更多
Vitamin D receptor （VDR） polymorphisms have been studied as potential contributors to multiple sclerosis （MS）. However, published studies differ with respect to study design and the significance of the effects det...Vitamin D receptor （VDR） polymorphisms have been studied as potential contributors to multiple sclerosis （MS）. However, published studies differ with respect to study design and the significance of the effects detected. The aim of this study was to quantify the magnitude of the risk associated with the Taql, Bsml, Apal and Fold VDR polymorphisms in MS using a meta-analysis approach. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we conducted a systematic search and meta-analysis of the literature. Subgroup analyses were performed to detect potential sources of heterogeneity from the selected study characteristics. The stability of the summary risk was evaluated using sensitivity analyses. The meta-analysis included a total of 3300 cases and 3194 controls from 13 case- control studies. There were no significant associations found between Taql and Bsml polymorphisms and MS risk. The association between the Apal polymorphism and MS risk was significant in the homozygous and codominant models （P=0.013 and P=0.031, respectively）, suggesting that the AA Apal genotype might be a significant MS risk factor. Publication year and age significantly affected the association between Taql polymorphisms and MS （P=0.014 and P=0.010, respectively）, which indicates a protective effect of the major T allele. The AA Apal and FF Fold genotypes are significant risk factors for MS. The association between the Taql polymorphism and MS risk is significantly affected by study characteristics.展开更多
文摘Background： Breast cancer is the most common type of cancer among females. Genetic polymorphisms might have a role in carcinogencsis. The aim of this study was to determine whether C to T base substitution within Taql Vitamin D receptor （VDR） gene （rs731236） in exon 9 was a risk factor among patients with breast cancer. Methods： Peripheral blood was drawn from 122 Jordanian breast cancer patients and 100 healthy Jordanian volunteers in Al-Bashecr Hospital during the summer months （from June to November of 2013, 2014, and 2015）. DNA was amplified using polymerase chain reaction （PCR）, followed by Taql restriction enzyme digestion. Quantification of serum 25-hydroxy Vitamin D （25[OH]D） level was determined by competitive immunoassay Elecsys. Results： Genotypic frequencies for Taql TT, Tt, and tt genotypes were 41%, 46%, and 13% for breast cancer compared to 42%, 50%. and 8% for control, respectively. Vitamin D serum level was significantly lower in the breast cancer patients （8.1 ± 0.3 ng/ml） compared to the control group （21.2± 0.6 ng/ml; P =0.001）. This study showed an inverse association between 25（OH）D serum level and brcasl cancer risk （odds ratio [OR], 22.72, 95% confidence interval [C/], 10.06 51.29）. Conclusions： An inverse association was found between 25（OH）D serum level and breast cancer risk. Statistical difference was also found between different VDR Taql genotypes and circulating levels of 25（OH）D among Jordanian females with breast cancer.
文摘Vitamin D receptor （VDR） polymorphisms have been studied as potential contributors to multiple sclerosis （MS）. However, published studies differ with respect to study design and the significance of the effects detected. The aim of this study was to quantify the magnitude of the risk associated with the Taql, Bsml, Apal and Fold VDR polymorphisms in MS using a meta-analysis approach. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines, we conducted a systematic search and meta-analysis of the literature. Subgroup analyses were performed to detect potential sources of heterogeneity from the selected study characteristics. The stability of the summary risk was evaluated using sensitivity analyses. The meta-analysis included a total of 3300 cases and 3194 controls from 13 case- control studies. There were no significant associations found between Taql and Bsml polymorphisms and MS risk. The association between the Apal polymorphism and MS risk was significant in the homozygous and codominant models （P=0.013 and P=0.031, respectively）, suggesting that the AA Apal genotype might be a significant MS risk factor. Publication year and age significantly affected the association between Taql polymorphisms and MS （P=0.014 and P=0.010, respectively）, which indicates a protective effect of the major T allele. The AA Apal and FF Fold genotypes are significant risk factors for MS. The association between the Taql polymorphism and MS risk is significantly affected by study characteristics.
