目的研究马来酸罗格列酮对大鼠Tan is mRNA表达及血浆白细胞介素-6(IL-6)水平的影响。方法W istar大鼠24只,随机分为模型组(A)、给药组(B)和对照组(C),每组8只。A、B两组高糖高脂喂养6周建立胰岛素抵抗模型,C组常规饲喂。模型制作成功后...目的研究马来酸罗格列酮对大鼠Tan is mRNA表达及血浆白细胞介素-6(IL-6)水平的影响。方法W istar大鼠24只,随机分为模型组(A)、给药组(B)和对照组(C),每组8只。A、B两组高糖高脂喂养6周建立胰岛素抵抗模型,C组常规饲喂。模型制作成功后B组喂食马来酸罗格列酮,4 mg.kg-1.d-1;A组喂以常规饲料。两周后所有大鼠禁食12 h后称重,测空腹血糖(FBG)、空腹胰岛素(FINS),并按Hom a公式计算胰岛素抵抗指数(IR);应用RT-PCR方法测定大鼠肝组织Tan is mRNA的表达;ELISA法测定IL-6水平。结果与C组相比,A组大鼠血清IL-6水平明显升高(P<0.05)。Tan is mRNA的表达明显增强(P<0.05);B组大鼠血清IL-6水平、Tan is mRNA的表达较A组明显降低(P<0.05)。多元回归分析显示,IL-6与FINS、IR正相关(r1=0.633,P=0.014;r2=0.638,P=0.015),Tan is mRNA的表达与IL-6、FINS、IR正相关(r1=0.606,P=0.022;r2=0.651,P=0.012;r3=0.584,P=0.028)。结论Tan is mRNA的高表达可能是胰岛素抵抗和糖尿病的发生机制之一,马来酸罗格列酮可有效改善胰岛素抵抗,降低血浆IL-6水平及Tan ismRNA表达。展开更多
社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观...社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观念、情感和行为模式。研究表明,社会情感能力不仅对个体的学业、事业发展和身心健康具有重要影响,还是减少反社会行为、促进社会和谐、提升幸福感的重要因素。初中生阶段是社会情感能力发展的关键阶段,本文以社会情感学习理论框架(SEL)为理论基础,在学校教育中对初中生社会情感能力的培养进行探索,并以心理课堂为例,以期为素质教育及学生非认知能力的培养提供理论基础。Social-emotional competence is a component of the core competence of middle school students facing the 21st century, which has an important impact on individual’s future achievement and mental health. Social affective ability learning is a comprehensive ability gradually formed by individuals through formal and informal learning, which is related to emotion management, communication and cooperation, effective cognition and achievement of goals, etc. It is manifested in the continuous development of ideas, emotions and behavior patterns. Studies have shown that social-emotional ability not only has an important impact on individual’s academic and career development and physical and mental health, but also an important factor to reduce anti-social behavior, promote social harmony and enhance happiness. The stage of junior high school students is the key stage of the development of social-emotional ability. Based on the theoretical framework of social-emotional learning (SEL), this paper explores the cultivation of junior high school students’ social-emotional ability in school education, and takes psychological classroom as an example, in order to provide a theoretical basis for quality education and the cultivation of students’ non-cognitive ability.展开更多
BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitorin...BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitoring protein synthesis and processing unfolded or misfolded proteins in actively growing tumor cells.However,the role of the multiple ERAD complexes in liver cancer remains unclear.AIM To elucidate the effects of SEL1L-mediated ERAD on Huh7 and explore the underlying mechanisms in vivo and in vitro.METHODS Huh7 cells were treated with ERAD inhibitor to identify ERAD’s role.Cell counting kit-8,5-ethynyl-2’-deoxyuridine and colony formation experiments were performed.Apoptosis level and migration ability were assessed using fluorescence activated cell sorting and Transwell assay,respectively.Huh7 SEL1L knockout cell line was established via clustered regularly interspaced short palindromic repeats,proliferation,apoptosis,and migration were assessed through previous experiments.The role of SEL1L in vivo and the downstream target of SEL1L were identified using Xenograft and mass spectrometry,respectively.RESULTS The ERAD inhibitor suppressed cell proliferation and migration and promoted apoptosis.SEL1L-HRD1 significantly influenced Huh7 cell growth.SEL1L knockout suppressed tumor cell proliferation and migration and enhanced apoptosis.Mass spectrometry revealed EXT2 is a primary substrate of ERAD.SEL1L knockout significantly increased the protein expression of EXT2.Furthermore,EXT2 knockdown partially restored the effect of SEL1L knockout.CONCLUSION ERAD inhibition suppressed the proliferation and migration of Huh7 and promoted its apoptosis.EXT2 plays an important role and ERAD might be a potential treatment for Huh7 hepatocellular carcinoma.展开更多
目的探讨SEL1L蛋白、BCL-2蛋白在弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织和细胞株SUDHL-4、LY-10中的表达及意义。方法采用免疫组化法检测123例DLBCL组织中SEL1L、BCL-2蛋白的表达及60例反应性淋巴组织增生(reacti...目的探讨SEL1L蛋白、BCL-2蛋白在弥漫大B细胞淋巴瘤(diffuse large B cell lymphoma,DLBCL)组织和细胞株SUDHL-4、LY-10中的表达及意义。方法采用免疫组化法检测123例DLBCL组织中SEL1L、BCL-2蛋白的表达及60例反应性淋巴组织增生(reactive lymphoid hyperplasia,RLH)组织中SEL1L蛋白的表达。应用免疫细胞化学及Western blot法检测SEL1L蛋白在DLBCL细胞株SUDHL-4及LY-10中的表达。结果 SEL1L蛋白在DLBCL中的高表达率明显高于RLH(69.9%vs 25.0%,P<0.05),其表达与各临床病理特征均无关。BCL-2蛋白在DLBCL中的阳性率为83.7%,与肿瘤原发位置、免疫表型及Ann Arbor分期有关(P<0.05);SEL1L蛋白与BCL-2蛋白在DLBCL中的表达呈正相关(r=0.227,P<0.05)。SEL1L蛋白在SUDHL-4及LY-10中均呈阳性。结论 SEL1L与BCL-2在DLBCL中均呈高表达,提示SEL1L在DLBCL的发生、发展中可能起重要作用,并可能与BCL-2表达产生协同作用。展开更多
在30万t/a Hostalen淤浆法聚乙烯生产装置中,采用国产SEL催化剂试生产了双峰聚乙烯薄膜料(牌号为HM-9455 F 1)。结果表明:试用SEL催化剂期间,装置运行平稳;SEL催化剂的组成、微观结构与参比催化剂(牌号为THT)相近;与参比催化剂相比,SEL...在30万t/a Hostalen淤浆法聚乙烯生产装置中,采用国产SEL催化剂试生产了双峰聚乙烯薄膜料(牌号为HM-9455 F 1)。结果表明:试用SEL催化剂期间,装置运行平稳;SEL催化剂的组成、微观结构与参比催化剂(牌号为THT)相近;与参比催化剂相比,SEL催化剂的聚合活性提高了17%,氢气单耗和副产物蜡产量分别降低了6.6%,9.5%;由SEL催化剂生产的双峰聚乙烯薄膜料,粒径分布集中在>63～250μm。展开更多
文摘目的研究马来酸罗格列酮对大鼠Tan is mRNA表达及血浆白细胞介素-6(IL-6)水平的影响。方法W istar大鼠24只,随机分为模型组(A)、给药组(B)和对照组(C),每组8只。A、B两组高糖高脂喂养6周建立胰岛素抵抗模型,C组常规饲喂。模型制作成功后B组喂食马来酸罗格列酮,4 mg.kg-1.d-1;A组喂以常规饲料。两周后所有大鼠禁食12 h后称重,测空腹血糖(FBG)、空腹胰岛素(FINS),并按Hom a公式计算胰岛素抵抗指数(IR);应用RT-PCR方法测定大鼠肝组织Tan is mRNA的表达;ELISA法测定IL-6水平。结果与C组相比,A组大鼠血清IL-6水平明显升高(P<0.05)。Tan is mRNA的表达明显增强(P<0.05);B组大鼠血清IL-6水平、Tan is mRNA的表达较A组明显降低(P<0.05)。多元回归分析显示,IL-6与FINS、IR正相关(r1=0.633,P=0.014;r2=0.638,P=0.015),Tan is mRNA的表达与IL-6、FINS、IR正相关(r1=0.606,P=0.022;r2=0.651,P=0.012;r3=0.584,P=0.028)。结论Tan is mRNA的高表达可能是胰岛素抵抗和糖尿病的发生机制之一,马来酸罗格列酮可有效改善胰岛素抵抗,降低血浆IL-6水平及Tan ismRNA表达。
文摘社会情感能力是中学生面向21世纪核心竞争力的构成要素,对个体未来成就以及心理健康有重要影响。社会情感能力学习是个体通过正式和非正式学习,逐渐形成的与情绪管理、交流合作,有效认知并达成目标等相关的综合能力,表现为不断发展的观念、情感和行为模式。研究表明,社会情感能力不仅对个体的学业、事业发展和身心健康具有重要影响,还是减少反社会行为、促进社会和谐、提升幸福感的重要因素。初中生阶段是社会情感能力发展的关键阶段,本文以社会情感学习理论框架(SEL)为理论基础,在学校教育中对初中生社会情感能力的培养进行探索,并以心理课堂为例,以期为素质教育及学生非认知能力的培养提供理论基础。Social-emotional competence is a component of the core competence of middle school students facing the 21st century, which has an important impact on individual’s future achievement and mental health. Social affective ability learning is a comprehensive ability gradually formed by individuals through formal and informal learning, which is related to emotion management, communication and cooperation, effective cognition and achievement of goals, etc. It is manifested in the continuous development of ideas, emotions and behavior patterns. Studies have shown that social-emotional ability not only has an important impact on individual’s academic and career development and physical and mental health, but also an important factor to reduce anti-social behavior, promote social harmony and enhance happiness. The stage of junior high school students is the key stage of the development of social-emotional ability. Based on the theoretical framework of social-emotional learning (SEL), this paper explores the cultivation of junior high school students’ social-emotional ability in school education, and takes psychological classroom as an example, in order to provide a theoretical basis for quality education and the cultivation of students’ non-cognitive ability.
基金Supported by the National Natural Science Foundation of China,No.82241219,No.82127808 and No.81921004The Shenzhen Science and Technology Program,No.JCYJ20210324120813037.
文摘BACKGROUND Proteins play a central role in regulating biological functions,and various pathways regulate their synthesis and secretion.Endoplasmic reticulum-associated protein degradation(ERAD)is crucial for monitoring protein synthesis and processing unfolded or misfolded proteins in actively growing tumor cells.However,the role of the multiple ERAD complexes in liver cancer remains unclear.AIM To elucidate the effects of SEL1L-mediated ERAD on Huh7 and explore the underlying mechanisms in vivo and in vitro.METHODS Huh7 cells were treated with ERAD inhibitor to identify ERAD’s role.Cell counting kit-8,5-ethynyl-2’-deoxyuridine and colony formation experiments were performed.Apoptosis level and migration ability were assessed using fluorescence activated cell sorting and Transwell assay,respectively.Huh7 SEL1L knockout cell line was established via clustered regularly interspaced short palindromic repeats,proliferation,apoptosis,and migration were assessed through previous experiments.The role of SEL1L in vivo and the downstream target of SEL1L were identified using Xenograft and mass spectrometry,respectively.RESULTS The ERAD inhibitor suppressed cell proliferation and migration and promoted apoptosis.SEL1L-HRD1 significantly influenced Huh7 cell growth.SEL1L knockout suppressed tumor cell proliferation and migration and enhanced apoptosis.Mass spectrometry revealed EXT2 is a primary substrate of ERAD.SEL1L knockout significantly increased the protein expression of EXT2.Furthermore,EXT2 knockdown partially restored the effect of SEL1L knockout.CONCLUSION ERAD inhibition suppressed the proliferation and migration of Huh7 and promoted its apoptosis.EXT2 plays an important role and ERAD might be a potential treatment for Huh7 hepatocellular carcinoma.
文摘在30万t/a Hostalen淤浆法聚乙烯生产装置中,采用国产SEL催化剂试生产了双峰聚乙烯薄膜料(牌号为HM-9455 F 1)。结果表明:试用SEL催化剂期间,装置运行平稳;SEL催化剂的组成、微观结构与参比催化剂(牌号为THT)相近;与参比催化剂相比,SEL催化剂的聚合活性提高了17%,氢气单耗和副产物蜡产量分别降低了6.6%,9.5%;由SEL催化剂生产的双峰聚乙烯薄膜料,粒径分布集中在>63～250μm。
