Radix codonopsis(党参)，which is derived fromCodonopsis pilosula, C.pilosula subsp. Modesta andC. Tangshen(ChP,2000),has been used as a remedy fora decrease of appetite, psychoneurosis, fatigue, dyspepsiaetc.or as ...Radix codonopsis(党参)，which is derived fromCodonopsis pilosula, C.pilosula subsp. Modesta andC. Tangshen(ChP,2000),has been used as a remedy fora decrease of appetite, psychoneurosis, fatigue, dyspepsiaetc.or as a substitute for Panax ginseng in traditionalChinese medicine. Relating to the constituents of theseherbs, triterpenes, phytosterols, furaldehyde, sesquitesand some phenolic glycosides have been reported, butnone of these compounds seems to be responsible for thebiological activities of this drug. As regards the quality evaluation of Radix Codonopsis, a HPLC analysis of atractylenolide Ⅲ has been described, but this sesquiterpene lactone exists in a very low content and is difficultfor conventional analysis. The determination of polysaccharide also met limitation because of its non-specialization. In this paper, we report the HPLC/MS fingerprintsanalysis of phelolic glycosides from Radix Codonopsis including three species.展开更多
Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the acti...Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the active composition of C.pilosula lacks systematic research.This study integrates the HPLC and UV-Vis methodologies to evaluate the quality of C.pilosula from commodity specification grades and different origins,and it explores the correlation between ecological factors and production origins with active components.Here,network pharmacology is used to determine that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The HPLC and UV-Vis methods were employed to quantitatively analyse the levels of five active compounds from different origins and commodity specification grades.Ecological factors were collected from the different production origins with ArcGIS,and correlation analysis was conducted between these factors and the active components of C.pilosula to identify the key ecological influences that drive the accumulation of active compounds.Results showed that network pharmacology analyses indicated that the active components of C.pilosula,including lobetyolin,syringin,and tangshenoside I,bind to targets and exhibit antioxidant and antipulmonary fibrosis effects.Differences in the contents of active components across three commodity specification grades were not significant.The contents of active components in C.pilosula showed differences with varying origins,with the most variation observed in soluble sugar content,and notable variations are also observed in the levels of lobetyolin,syringin,and tangshenoside I,which could serve as potential biomarkers for different origins.Additionally,ecological factors influenced the accumulation of C.pilosula’s active components.The contents of soluble sugars and tangshenoside I were positively correlated with temperature and precipitation.Our study evaluated the active components of C.pilosula,and findings show that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The differences in the quality of C.pilosula across varying commodity specification grades are not significant.The different contents of C.pilosula across varying origins are significant,with soluble sugars and glycosides serving as potential markers for distinguishing C.pilosula from different origins.Moreover,ecological factors drove the accumulation of C.pilosula components.Soluble sugars and tangshenoside I content were particularly influenced by temperature and precipitation.Sand content and electrical conductivity significantly correlated with syringin,whereas organic carbon negatively influenced total flavonoids.This research provides a theoretical basis for the selection of the C.pilosula growing area and lays a foundation for the study of the C.pilosula quality standard.展开更多
文摘Radix codonopsis(党参)，which is derived fromCodonopsis pilosula, C.pilosula subsp. Modesta andC. Tangshen(ChP,2000),has been used as a remedy fora decrease of appetite, psychoneurosis, fatigue, dyspepsiaetc.or as a substitute for Panax ginseng in traditionalChinese medicine. Relating to the constituents of theseherbs, triterpenes, phytosterols, furaldehyde, sesquitesand some phenolic glycosides have been reported, butnone of these compounds seems to be responsible for thebiological activities of this drug. As regards the quality evaluation of Radix Codonopsis, a HPLC analysis of atractylenolide Ⅲ has been described, but this sesquiterpene lactone exists in a very low content and is difficultfor conventional analysis. The determination of polysaccharide also met limitation because of its non-specialization. In this paper, we report the HPLC/MS fingerprintsanalysis of phelolic glycosides from Radix Codonopsis including three species.
基金funded by the National Key R&D Plan(2022YFC3501804)Fundamental Research Funds for the Central Public Welfare Research Institutes(No.ZZ13-YQ-049)National Natural Science Foundation of China(ZXKT22001).
文摘Codonopsis pilosula is a major Qi-tonifying medicinal herb,and its active composition is analyzed systematically.However,the relationship between its production origins and commodity specification grades with the active composition of C.pilosula lacks systematic research.This study integrates the HPLC and UV-Vis methodologies to evaluate the quality of C.pilosula from commodity specification grades and different origins,and it explores the correlation between ecological factors and production origins with active components.Here,network pharmacology is used to determine that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The HPLC and UV-Vis methods were employed to quantitatively analyse the levels of five active compounds from different origins and commodity specification grades.Ecological factors were collected from the different production origins with ArcGIS,and correlation analysis was conducted between these factors and the active components of C.pilosula to identify the key ecological influences that drive the accumulation of active compounds.Results showed that network pharmacology analyses indicated that the active components of C.pilosula,including lobetyolin,syringin,and tangshenoside I,bind to targets and exhibit antioxidant and antipulmonary fibrosis effects.Differences in the contents of active components across three commodity specification grades were not significant.The contents of active components in C.pilosula showed differences with varying origins,with the most variation observed in soluble sugar content,and notable variations are also observed in the levels of lobetyolin,syringin,and tangshenoside I,which could serve as potential biomarkers for different origins.Additionally,ecological factors influenced the accumulation of C.pilosula’s active components.The contents of soluble sugars and tangshenoside I were positively correlated with temperature and precipitation.Our study evaluated the active components of C.pilosula,and findings show that lobetyolin,syringin,and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress.The differences in the quality of C.pilosula across varying commodity specification grades are not significant.The different contents of C.pilosula across varying origins are significant,with soluble sugars and glycosides serving as potential markers for distinguishing C.pilosula from different origins.Moreover,ecological factors drove the accumulation of C.pilosula components.Soluble sugars and tangshenoside I content were particularly influenced by temperature and precipitation.Sand content and electrical conductivity significantly correlated with syringin,whereas organic carbon negatively influenced total flavonoids.This research provides a theoretical basis for the selection of the C.pilosula growing area and lays a foundation for the study of the C.pilosula quality standard.
