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小麦TaHTAS-5A基因的克隆、表达分析及亚细胞定位 被引量:4
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作者 刘燕 赵毅 +3 位作者 吕千 张丽 李立群 李学军 《麦类作物学报》 CAS CSCD 北大核心 2018年第10期1137-1145,共9页
非生物胁迫对小麦的生长发育具有不良影响,克隆与非生物胁迫相关的基因,并对其结构及表达特性进行分析,可以为进一步探索小麦的抗逆机制和抗逆育种奠定基础。本研究利用同源克隆技术从普通小麦品种中国春中克隆了水稻E3泛素连接酶基因Os... 非生物胁迫对小麦的生长发育具有不良影响,克隆与非生物胁迫相关的基因,并对其结构及表达特性进行分析,可以为进一步探索小麦的抗逆机制和抗逆育种奠定基础。本研究利用同源克隆技术从普通小麦品种中国春中克隆了水稻E3泛素连接酶基因OsHTAS的同源基因TaHTAS-5A(GeneBank登录号:MF967573),并利用生物信息学方法对其基因序列特征进行分析;利用qRT-PCR技术对该基因在小麦不同组织及不同逆境胁迫下的表达模式进行分析;同时,对该基因的表达产物进行了亚细胞定位。结果表明,小麦TaHTAS-5A基因含有220bp的5′UTR、1 242bp的ORF以及126bp的3′UTR,共编码413个氨基酸;基因组分析表明,该基因全长3 819bp,共包含4个外显子和3个内含子;缺体-四体定位表明该基因位于小麦5A染色体上;蛋白结构分析显示,TaHTAS-5A蛋白的N端包含四个跨膜结构域,C端包含一个E3泛素连接酶特有的RING finger保守结构域,具有植物E3泛素连接酶的结构特征;qRT-PCR结果显示,TaHTAS-5A对高温、低温和ABA都有响应,对干旱和盐响应不敏感;TaHTAS-5A在小麦的根、茎、叶和幼穗等不同组织中均有表达,但表达量有差异,在叶和穗中的表达量明显高于根部和茎部;亚细胞定位结果表明,TaHTAS-5A位于细胞膜上。本研究为进一步探究小麦TaHTAS-5A基因的功能奠定了基础,也为小麦非生物胁迫抗性改良提供了一定的理论依据。 展开更多
关键词 小麦 tahtaS-5A 非生物胁迫 表达分析 亚细胞定位
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Comparison of the Groundwater Quality in the West Tahta Area, Upper Egypt in 1989 and 2011 被引量:1
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作者 Ismail Esam Faid Abdalla +1 位作者 Niesner Erich Mauritsch Hermann 《Journal of Environmental Protection》 2012年第11期1442-1457,共16页
Egypt is characterized by arid climate with rare rainfall that means surface water. The River Nile is the main source of water supply. The study area is located in Upper Egypt in the western desert. The area under inv... Egypt is characterized by arid climate with rare rainfall that means surface water. The River Nile is the main source of water supply. The study area is located in Upper Egypt in the western desert. The area under investigation is subjected to intensive development in the last twenty years, clearly seen in the change of the agriculture and urban areas. The rapid increase of the population in Egypt especially in the upper part leads to an increasing demand for houses on the old cultivated land lead to the migration towards the desert and reclaimed new areas. These new reclaimed areas are depending on the ground water. The extensive use of groundwater and increasing fertilizing system leads to the change of the water table, characterization and quality. In this study we use the analysis of 30 groundwater samples collected and analyzed in 1989 and 28 groundwater samples collected and analyzed in 2011 from the same area to identify the change in the quality of the ground water. According the hydrogeologic data which were collected from the study area in 1989 and 2011 we found a changing water table decreasing, from 1 to 48 m in 1989 and 5 to 60 m in 2011. The water level is decreasing in the same direction towards the River Nile. The sufficient recharge and leaching and dissolution of the aquifer materials due to extensive use lead to increase in TDS which reaching to 4453 ppm in 2011. The water type in the 1989 samples is mainly NaHCO3 and in 2011 samples Na2SO4. Grid classification shows that the water samples collected in 1989 are mainly sodium bicarbonate and sodium chloride extending to sodium sulphate while the water samples collected in 2011 are mainly sodium sulphate and sodium chloride. Most of the 1989 groundwater samples are suitable for drinking purposes while majority of the 2011 groundwater samples are not suitable due to high salinity. 展开更多
关键词 Under GROUNDWATER WITHDRAWAL WEST tahta Evaluation of GROUNDWATER
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