The SDS-sedimentation volume(SSV)is a critical indicator for assessing wheat gluten quality and is widely used when evaluating wheat processing quality.However,the molecular mechanisms regulating SSV remain poorly und...The SDS-sedimentation volume(SSV)is a critical indicator for assessing wheat gluten quality and is widely used when evaluating wheat processing quality.However,the molecular mechanisms regulating SSV remain poorly understood.In this study,we performed an analysis of quantitative trait loci(QTLs)for SSV using a recombinant inbred line(RIL)population derived from a cross between TAA10 and XX329,and identified four environmentally stable QTLs located on chromosomes 1D,2D,4D,and 6D.Among them,the effects of Qssv.cau-1D and Qssv.cau-6D were likely to be explained by genome variations at the Glu-D1 and Gli-D2 loci.We fine mapped Qssv.cau-2D to the candidate causal gene TaSED,encoding a nucleolar protein.Gene-edited TaSED knockout mutants(tased)had a lower SSV,while TaSED overexpression lines showed a higher SSV.We demonstrated that TaSED interacted with the transcription factor TaSPA to enhance its transcriptional activation activity of glutenin and gliadin,whose expression was downregulated in tased and upregulated in TaSED-OE plants,with corresponding differences in glutenin and gliadin content compared with the wild-type.A molecular marker sedTX was further developed based on a nonsynonymous mutation of the parents in TaSED that could be used to identify haplotypes with high SSV effectively.Our findings elucidate a molecular mechanism governing SSV and reveal valuable variants with promising applications for improving wheat quality.展开更多
The structural features and three-dimensional nature of the charge density wave （CDW） state of the layered chalcogenide 1T-TaSe2-xTex （0≤x≤2.0） are characterized by Cs-corrected transmission electron microscopy ...The structural features and three-dimensional nature of the charge density wave （CDW） state of the layered chalcogenide 1T-TaSe2-xTex （0≤x≤2.0） are characterized by Cs-corrected transmission electron microscopy measurements. Notable changes of both average structure and the CDW state arising from Te substitution for Se are clearly demonstrated in samples with x〉0.3. The commensurate CDW state characterized by the known star-of-David clustering in the 1T-TaSe2 crystal becomes visibly unstable with Te substitution and vanishes when x=0.3. The 1T-TaSe2-xTex （0.3≤x≤1.3） samples generally adopt a remarkable incommensurate CDW state with monoclinic distortion, which could be fundamentally in correlation with the strong qq-dependent electron-phonon coupling-induced period-lattice-distortion as identified in TaTe22. Systematic analysis demonstrates that the occurrence of superconductivity is related to the suppression of the commensurate CDW phase and the presence of discommensuration is an evident structural feature observed in the superconducting samples.展开更多
基金supported financially by STI2030-Major Projects(No.2023ZD0406903)the China Postdoctoral Science Foundation(No.2023M743818)the China Postdoctoral Science Foundation(No.GZC20233050).
文摘The SDS-sedimentation volume(SSV)is a critical indicator for assessing wheat gluten quality and is widely used when evaluating wheat processing quality.However,the molecular mechanisms regulating SSV remain poorly understood.In this study,we performed an analysis of quantitative trait loci(QTLs)for SSV using a recombinant inbred line(RIL)population derived from a cross between TAA10 and XX329,and identified four environmentally stable QTLs located on chromosomes 1D,2D,4D,and 6D.Among them,the effects of Qssv.cau-1D and Qssv.cau-6D were likely to be explained by genome variations at the Glu-D1 and Gli-D2 loci.We fine mapped Qssv.cau-2D to the candidate causal gene TaSED,encoding a nucleolar protein.Gene-edited TaSED knockout mutants(tased)had a lower SSV,while TaSED overexpression lines showed a higher SSV.We demonstrated that TaSED interacted with the transcription factor TaSPA to enhance its transcriptional activation activity of glutenin and gliadin,whose expression was downregulated in tased and upregulated in TaSED-OE plants,with corresponding differences in glutenin and gliadin content compared with the wild-type.A molecular marker sedTX was further developed based on a nonsynonymous mutation of the parents in TaSED that could be used to identify haplotypes with high SSV effectively.Our findings elucidate a molecular mechanism governing SSV and reveal valuable variants with promising applications for improving wheat quality.
基金Supported by the National Basic Research Program of China under Grant Nos 2015CB921300 and 2012CB821404the National Key Research and Development Program of China under Grant Nos 2016YFA0300300 and 2016YFA0300404+1 种基金the National Natural Science Foundation of China under Grant Nos 11474323,11604372,11274368,91221102,11190022,11674326 and 91422303the Strategic Priority Research Program(B)of the Chinese Academy of Sciences under Grant No XDB07020000
文摘The structural features and three-dimensional nature of the charge density wave （CDW） state of the layered chalcogenide 1T-TaSe2-xTex （0≤x≤2.0） are characterized by Cs-corrected transmission electron microscopy measurements. Notable changes of both average structure and the CDW state arising from Te substitution for Se are clearly demonstrated in samples with x〉0.3. The commensurate CDW state characterized by the known star-of-David clustering in the 1T-TaSe2 crystal becomes visibly unstable with Te substitution and vanishes when x=0.3. The 1T-TaSe2-xTex （0.3≤x≤1.3） samples generally adopt a remarkable incommensurate CDW state with monoclinic distortion, which could be fundamentally in correlation with the strong qq-dependent electron-phonon coupling-induced period-lattice-distortion as identified in TaTe22. Systematic analysis demonstrates that the occurrence of superconductivity is related to the suppression of the commensurate CDW phase and the presence of discommensuration is an evident structural feature observed in the superconducting samples.
