Bread wheat(Triticum aestivum L.)is one of the most important staple crops worldwide.The phytohormone auxin plays critical roles in the regulation of plant growth and development.However,only a few auxin-related genes...Bread wheat(Triticum aestivum L.)is one of the most important staple crops worldwide.The phytohormone auxin plays critical roles in the regulation of plant growth and development.However,only a few auxin-related genes have been genetically demonstrated to be involved in the control of plant architecture in wheat thus far.In this study,we characterized an auxinrelated gene in wheat,TaIAA15,and found that its ectopic expression in rice decreased the plant height and increased the leaf angle.Correlation analysis indicated that TaIAA15-3B was associated with plant height(Ph),spike length(SL)and 1000-grain weight(TGW)in wheat,and Hap-II of TaIAA15-3B was the most favored allele and selected by modern breeding in China.This study sheds light on the role of auxin signaling on wheat plant architecture as well as yield related traits.展开更多
Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regula...Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regulatory function of miR319 in common wheat plant height.Repression of tae-miR319 through short tandem target mimics(STTM) caused an increased plant height,while overexpression(OE) of tae-miR319 had the opposite effect.Overexpressing a miR319-resistant target gene TaPCF8(rTaPCF8),increased plant height.TaPCF8 acted as a transcription repressor of downstream genes TaIAAs,which interact physically with TaSPL14.The significant differences of indole-3-acetic acid(IAA) contents indicate the involvement of auxin pathway in miR319-mediated plant height regulation.Finally,we identified two TaPCF8 haplotypes in global wheat collections.TaPCF8-5A-Hap2,as per association and evolution examinations,was subjected to strong substantial selection throughout wheat breeding.This haplotype,associated with shorter plant height,aligns with global breeding requirements.Consequently,in high-yield wheat breeding,we proposed a potential molecular marker for marker-assisted selection(MAS).Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.展开更多
基金supported by the National Basic Research Program of China(2016YFD0100102 and 2016YFD0100302)。
文摘Bread wheat(Triticum aestivum L.)is one of the most important staple crops worldwide.The phytohormone auxin plays critical roles in the regulation of plant growth and development.However,only a few auxin-related genes have been genetically demonstrated to be involved in the control of plant architecture in wheat thus far.In this study,we characterized an auxinrelated gene in wheat,TaIAA15,and found that its ectopic expression in rice decreased the plant height and increased the leaf angle.Correlation analysis indicated that TaIAA15-3B was associated with plant height(Ph),spike length(SL)and 1000-grain weight(TGW)in wheat,and Hap-II of TaIAA15-3B was the most favored allele and selected by modern breeding in China.This study sheds light on the role of auxin signaling on wheat plant architecture as well as yield related traits.
基金financially supported by the Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-CSCB-202401)the National Natural Science Foundation of China(32172045)Beijing Natural Science Foundation(6242032)。
文摘Wheat culms,comprising four to six internodes,are critically involved in determining plant height and lodging resistance,essential factors for field performance and regional adaptability.This study revealed the regulatory function of miR319 in common wheat plant height.Repression of tae-miR319 through short tandem target mimics(STTM) caused an increased plant height,while overexpression(OE) of tae-miR319 had the opposite effect.Overexpressing a miR319-resistant target gene TaPCF8(rTaPCF8),increased plant height.TaPCF8 acted as a transcription repressor of downstream genes TaIAAs,which interact physically with TaSPL14.The significant differences of indole-3-acetic acid(IAA) contents indicate the involvement of auxin pathway in miR319-mediated plant height regulation.Finally,we identified two TaPCF8 haplotypes in global wheat collections.TaPCF8-5A-Hap2,as per association and evolution examinations,was subjected to strong substantial selection throughout wheat breeding.This haplotype,associated with shorter plant height,aligns with global breeding requirements.Consequently,in high-yield wheat breeding,we proposed a potential molecular marker for marker-assisted selection(MAS).Our findings offer fresh perspectives into the molecular mechanisms that underlie the miR319–TaPCF8 module's regulation of plant height by orchestrating auxin signaling and biosynthesis in wheat.
