Fuel cell stacks as the automotive power source can be severely poisoned by a trace amount of NOx in atmosphere,which makes it necessary to provide clean air for fuel cell vehicles.In this work,activating commercial a...Fuel cell stacks as the automotive power source can be severely poisoned by a trace amount of NOx in atmosphere,which makes it necessary to provide clean air for fuel cell vehicles.In this work,activating commercial activated carbons with K2CO3 for the large enhancement of NO capture was studied.K2CO3 modified activated carbons(K2CO3 ACs)were prepared by impregnating activate carbons in K2CO3 solution under ultrasound treatment,followed by temperature programmed baking at 800 oC.The dynamic NO flow tests on K2CO3 ACs at room temperature indicated that NO adsorption capacity reached the maximum(96 mg/g)when K2CO3 loading was 19.5 wt%,which corresponded to a specific surface area of 1196.1 m2/g and total pore volume of 0.70 cm3/g.The ten-fold enhancement of NO adsorption on K2CO3 ACs compared to the unimpregnated activated carbon was mainly attributed to the formation of potassium nitrite,which was confirmed by FTIR and temperature programmed desorption measurements.Regeneration tests of NO adsorption on the optimum sample revealed that 76%of the NO adsorption capacity could be remained after the fourth cycle.展开更多
目的构建八年制临床医学专业学生(以下酌情简称八年制医学生)置信职业行为(entrustable professional activities,EPAs)框架,以解决传统的临床胜任力评价抽象、模糊的问题,并弥合胜任力导向教育与临床实践之间的差距。方法基于EPAs理论...目的构建八年制临床医学专业学生(以下酌情简称八年制医学生)置信职业行为(entrustable professional activities,EPAs)框架,以解决传统的临床胜任力评价抽象、模糊的问题,并弥合胜任力导向教育与临床实践之间的差距。方法基于EPAs理论模型,采用文献分析方法和德尔菲专家咨询方法确定八年制医学生EPAs框架核心指标,运用SPSS 26.0软件进行统计学处理。结果选择28名临床教学专家和教学管理专家,针对八年制医学生EPAs框架各项指标进行2轮德尔菲函询。2轮专家函询的有效问卷回收率均为100.0%(28/28)。第1轮专家函询针对置信职业行为重要性和可行性评分的W值分别为0.127和0.164(均P<0.05),第2轮专家函询的置信职业行为重要性和可行性评分的W值分别为0.228和0.140(均P<0.05)。经过2轮专家函询后,初步构建了八年制医学生EPAs框架,具体包括19项指标和67个指标内涵的观测评价要点。结论本研究初步构建了八年制医学生EPAs框架,实现了临床任务与能力标准的精准映射,为八年制医学生临床能力培养提供了可量化、可追踪的评价标准,对深化医学教育改革具有参考价值。展开更多
Background:Thimerosal is a mercury-containing preservative widely used in vaccines.This study aimed to investigate its potential antitumor effects and mechanisms in solid malignancies,particularly colorectal cancer(CR...Background:Thimerosal is a mercury-containing preservative widely used in vaccines.This study aimed to investigate its potential antitumor effects and mechanisms in solid malignancies,particularly colorectal cancer(CRC)and melanoma.Methods:A combination of in vitro and in vivo approaches was employed.Cell proliferation,apoptosis,migration,and invasion were assessed using Cell Counting Kit-8(CCK-8),colony formation,ATP viability,Western blotting,flow cytometry,wound-healing and Transwell assays.Subcutaneous,lung metastases,and Azoxymethane/Dextran Sulfate Sodium Salt(AOM/DSS)-induced colitis-associated CRC models were established to examine antitumor efficacy and safety.The functional role of mercury ions was validated using structural analogues.Mechanistic studies included RNA sequencing,Western blot,and immunohistochemical analysis of CD8^(+)T cell infiltration.The synergistic effect with programmed cell death protein 1(PD-1)antibody therapy was also evaluated.Results:Thimerosal potently inhibited tumor growth(with IC_(50) values ranging from 0.1 to 1μM in vitro)and significantly prolonged survival without overt toxicity in vivo.Mechanistically,mercury ions were identified as critical functional sites mediating Thimerosal’s antitumor effects.Specifically,Thimerosal inhibited the phosphorylation of Janus kinase 1(JAK1)and signal transducer and activator of transcription 3(STAT3).Furthermore,it enhanced the infiltration of CD8^(+)T cells into the tumor microenvironment and synergistically augmented the efficacy of anti-PD-1 therapy.Conclusion:Thimerosal exerts dual antitumor roles by direct JAK1/STAT3 inhibition and immune modulation via CD8^(+)T cell recruitment.It represents a promising repurposed drug and immunotherapeutic adjuvant for CRC and melanoma.展开更多
基金Project(2018YFB0105303)supported by the Ministry of Science and Technology of ChinaProject(17DZ1200702)supported by the Shanghai Science and Technology Committee,China
文摘Fuel cell stacks as the automotive power source can be severely poisoned by a trace amount of NOx in atmosphere,which makes it necessary to provide clean air for fuel cell vehicles.In this work,activating commercial activated carbons with K2CO3 for the large enhancement of NO capture was studied.K2CO3 modified activated carbons(K2CO3 ACs)were prepared by impregnating activate carbons in K2CO3 solution under ultrasound treatment,followed by temperature programmed baking at 800 oC.The dynamic NO flow tests on K2CO3 ACs at room temperature indicated that NO adsorption capacity reached the maximum(96 mg/g)when K2CO3 loading was 19.5 wt%,which corresponded to a specific surface area of 1196.1 m2/g and total pore volume of 0.70 cm3/g.The ten-fold enhancement of NO adsorption on K2CO3 ACs compared to the unimpregnated activated carbon was mainly attributed to the formation of potassium nitrite,which was confirmed by FTIR and temperature programmed desorption measurements.Regeneration tests of NO adsorption on the optimum sample revealed that 76%of the NO adsorption capacity could be remained after the fourth cycle.
文摘目的构建八年制临床医学专业学生(以下酌情简称八年制医学生)置信职业行为(entrustable professional activities,EPAs)框架,以解决传统的临床胜任力评价抽象、模糊的问题,并弥合胜任力导向教育与临床实践之间的差距。方法基于EPAs理论模型,采用文献分析方法和德尔菲专家咨询方法确定八年制医学生EPAs框架核心指标,运用SPSS 26.0软件进行统计学处理。结果选择28名临床教学专家和教学管理专家,针对八年制医学生EPAs框架各项指标进行2轮德尔菲函询。2轮专家函询的有效问卷回收率均为100.0%(28/28)。第1轮专家函询针对置信职业行为重要性和可行性评分的W值分别为0.127和0.164(均P<0.05),第2轮专家函询的置信职业行为重要性和可行性评分的W值分别为0.228和0.140(均P<0.05)。经过2轮专家函询后,初步构建了八年制医学生EPAs框架,具体包括19项指标和67个指标内涵的观测评价要点。结论本研究初步构建了八年制医学生EPAs框架,实现了临床任务与能力标准的精准映射,为八年制医学生临床能力培养提供了可量化、可追踪的评价标准,对深化医学教育改革具有参考价值。
基金supported by the National Natural Science Foundation of China(82441036)Ganzhou Municipal Science and Technology Project(2022-RC1342)+3 种基金Guangdong Basic and Applied Basic Research Foundation(2022B1515130004)Key-Area Research and Development Program of Guangdong Province(2019B020234003)Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Cancer(2020B121201004)Open Project Fund Project of Guangdong Academy of Medical Sciences(YKY-KF202210).
文摘Background:Thimerosal is a mercury-containing preservative widely used in vaccines.This study aimed to investigate its potential antitumor effects and mechanisms in solid malignancies,particularly colorectal cancer(CRC)and melanoma.Methods:A combination of in vitro and in vivo approaches was employed.Cell proliferation,apoptosis,migration,and invasion were assessed using Cell Counting Kit-8(CCK-8),colony formation,ATP viability,Western blotting,flow cytometry,wound-healing and Transwell assays.Subcutaneous,lung metastases,and Azoxymethane/Dextran Sulfate Sodium Salt(AOM/DSS)-induced colitis-associated CRC models were established to examine antitumor efficacy and safety.The functional role of mercury ions was validated using structural analogues.Mechanistic studies included RNA sequencing,Western blot,and immunohistochemical analysis of CD8^(+)T cell infiltration.The synergistic effect with programmed cell death protein 1(PD-1)antibody therapy was also evaluated.Results:Thimerosal potently inhibited tumor growth(with IC_(50) values ranging from 0.1 to 1μM in vitro)and significantly prolonged survival without overt toxicity in vivo.Mechanistically,mercury ions were identified as critical functional sites mediating Thimerosal’s antitumor effects.Specifically,Thimerosal inhibited the phosphorylation of Janus kinase 1(JAK1)and signal transducer and activator of transcription 3(STAT3).Furthermore,it enhanced the infiltration of CD8^(+)T cells into the tumor microenvironment and synergistically augmented the efficacy of anti-PD-1 therapy.Conclusion:Thimerosal exerts dual antitumor roles by direct JAK1/STAT3 inhibition and immune modulation via CD8^(+)T cell recruitment.It represents a promising repurposed drug and immunotherapeutic adjuvant for CRC and melanoma.
