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Rearing Velocity Impacts on Landlocked Fall Chinook Salmon (<i>Oncorhynchus tshawytscha</i>) Growth, Condition, and Survival 被引量:4
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作者 Timothy M. Parker Michael E. Barnes 《Open Journal of Animal Sciences》 2014年第5期244-252,共9页
Juvenile landlocked Chinook salmon (Oncorhynchus tshawytscha) (mean ± SD initial weight 2.6 ± 0.7 g, fork length 6.3 ± 0.5) were reared in three different water velocities [0.5, 1.5 and 3.0 body length/... Juvenile landlocked Chinook salmon (Oncorhynchus tshawytscha) (mean ± SD initial weight 2.6 ± 0.7 g, fork length 6.3 ± 0.5) were reared in three different water velocities [0.5, 1.5 and 3.0 body length/s (BL/s)] for four weeks to determine possible effects of water velocity on growth, condition, and survival. Fish were sampled for weight, fork length, condition factor, hepatosomatic index (HSI), viscerosomatic index (VSI), and fin erosion after four weeks of feeding to satiation. At the end of the feeding trial, the fish were handled and transported to simulate stocking, with survival observed over the following 10 d. Following four weeks of feeding, fish reared in 0.5 and 1.5 BL/s had the same growth and food conversion ratio, but fish reared at 3.0 BL/s had a significant reduction in both metrics. Furthermore, fish reared at 1.5 BL/s had a significantly higher condition factor than fish reared in other treatments. No significant differences were found for HSI, VSI, fin erosion, or survival. The results from this study indicate that a moderate velocity (1.5 BL/s), which is necessary for circular tanks to be self-cleaning, is not detrimental to fish growth or condition, but a faster water velocity (3.0 BL/s) negatively affects fish growth and food utilization. 展开更多
关键词 CHINOOK Salmon ONCORHYNCHUS tshawytscha VELOCITY Circular Tanks
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大鳞大马哈鱼垂体生长激素的分离、纯化与鉴定 被引量:4
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作者 陈松林 Bernard Breton Pierre-YvesLeBail 《生物化学杂志》 CSCD 1992年第6期656-660,共5页
应用ConA-Sepharose 4B亲和层析、凝胶过滤及离子交换层析等技术从大鳞大马哈鱼(Oncorhynchus tshawytscha)垂体中分离纯化了具有生物活性的生长激素(sGH)。用放射受体测定法(RRA)检测sGH组分的生物活性,结果表明纯化的8GH制品具有与兔... 应用ConA-Sepharose 4B亲和层析、凝胶过滤及离子交换层析等技术从大鳞大马哈鱼(Oncorhynchus tshawytscha)垂体中分离纯化了具有生物活性的生长激素(sGH)。用放射受体测定法(RRA)检测sGH组分的生物活性,结果表明纯化的8GH制品具有与兔肝细胞GH受体结合的生物活性。用放射免疫测定法(RIA)和酶联免疫吸附测定法(ELISA)分别检测了另两种垂体激素-催乳激素(PRL)和促性腺激素(GTH)在纯化的sGH制品中的残留量均在0.5%以下。用SDS-聚丙烯酰胺凝胶电泳SDS-PAGE评价sGH制品的电泳纯度并测定了其分子量为22000左右。等电聚焦电泳表明该种鱼GH由等电点分别为6.3和6.6的两种形式的分子组成。 展开更多
关键词 生长激素 提纯 大马哈鱼属
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Impacts of Zebra Mussel Veliger Control Treatments on the Survival of Water-Hardened Landlocked Fall Chinook Salmon Eggs 被引量:1
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作者 Sierra Hillard Nathan Huysman Michael E. Barnes 《Natural Resources》 2019年第4期115-120,共6页
Several treatment options have been developed to minimize the spread of zebra mussel Dreissena polymorphaveligers (larvae) during fish transportation. However, the effect of these treatments on the survival of newly-f... Several treatment options have been developed to minimize the spread of zebra mussel Dreissena polymorphaveligers (larvae) during fish transportation. However, the effect of these treatments on the survival of newly-fertilized salmonid eggs has not been evaluated. This study examined the survival of water-hardened landlocked fall Chinook salmon Oncorhynchus tshawytscha eggs after one of four different treatments: 1) Control (no chemicals), 2) 100 mg/L formalin for two hours, 3) 750 mg/L of potassium chloride for one hour followed by 20 mg/L formalin for two hours, and 4) 750 mg/L of potassium chloride for one hour followed by 20 mg/L formalin for three hours. The 100 mg/L formalin treatment produced complete egg mortality. Survival to hatch was not significantly different among the other three treatments. Based on these results, the use of 750 mg/L potassium chloride for one hour followed by 20 mg/L formalin for three hours is recommended when moving Chinook salmon eggs from waters potentially infested with zebra mussels to hatcheries for incubation. 展开更多
关键词 Zebra Mussel DREISSENA polymorpha Landlocked FALL CHINOOK Salmon ONCORHYNCHUS tshawytscha
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Vertically-Suspended Environmental Enrichment Improves Growth of Landlocked Fall Chinook Salmon during Initial Hatchery Rearing 被引量:1
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作者 Nathan Huysman Jill M. Voorhees +1 位作者 Eric Krebs Michael E. Barnes 《Open Journal of Applied Sciences》 2020年第11期725-731,共7页
Environmental enrichment has been shown to improve juvenile salmonid growth during hatchery rearing. This study examined the effects of vertically-suspended environmental enrichment starting seven days after feed trai... Environmental enrichment has been shown to improve juvenile salmonid growth during hatchery rearing. This study examined the effects of vertically-suspended environmental enrichment starting seven days after feed training and continuing for the next 32 days of landlocked fall Chinook salmon (</span><i><span style="font-family:Verdana;">Oncorhynchus tshawytscha</span></i><span style="font-family:Verdana;">) rearing. At the end of this experiment, final tank weights, gain, percent gain, and feed conversion ratio were all significantly improved in the tanks of salmon with environmental enrichment compared to barren tanks, with a nearly 25% improvement in feed conversion ratio. Individual salmon in tanks with environmental enrichment were significantly longer and weighed significantly more than salmon from tanks without enrichment. Specific growth rate was also significantly improved with the use of enrichment. The use of vertically-suspended environmental enrichment to improve growth and rearing efficiency is recommended during the initial rearing of landlocked fall Chinook salmon. 展开更多
关键词 Oncorhynchus tshawytscha Environmental Enrichment Structure Initial Feeding
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Exercise and Structure Improve Juvenile Chinook Salmon Rearing Performance
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作者 Jill M. Voorhees Nathan Huysman +1 位作者 Eric Krebs Michael E. Barnes 《Open Journal of Marine Science》 2021年第2期80-91,共12页
This experiment evaluated the use of an exercise routine and vertically-sus<span style="font-family:Verdana;">-<span style="font-family:;" "=""><span style="font-... This experiment evaluated the use of an exercise routine and vertically-sus<span style="font-family:Verdana;">-<span style="font-family:;" "=""><span style="font-family:Verdana;">pended structure during juvenile landlocked fall Chinook salmon (<span style="font-family:Verdana;">Oncor<span style="font-family:Verdana;">hynchus tshawytscha<span style="font-family:Verdana;">;<span style="font-family:;" "=""> <span style="font-family:Verdana;">mean ± SE, initial weight 1.47 ± 0.03 g, total length 56.4 ± 0.4 mm) rearing. Four treatments were used: 1<span style="font-family:Verdana;">)<span style="font-family:Verdana;"> no exercise routine nor vertically-suspended structure, 2<span style="font-family:Verdana;">)<span style="font-family:Verdana;"> exercise and structure, 3<span style="font-family:Verdana;">)<span style="font-family:Verdana;"> exercise and no structure, and 4<span style="font-family:Verdana;">)<span style="font-family:;" "=""><span style="font-family:Verdana;"> no exercise and structure. <span style="font-family:Verdana;">Water velocities in tanks without exercise were 12 cm/s, where-as the exercise routine consisted of seven days at 12 cm/s followed by seven days at 18 cm/s. The structure was an array consisting of four vertically-suspended aluminum angles. Total tank gain and percent gain were significantly greater after 50 days in the tanks of salmon subjected to the exercise routine and structure compared to the three other treatments. <span style="font-family:Verdana;">Gain and percent gain were also significantly greater in the tanks receiving<span style="font-family:Verdana;"> structure without exercise compared to tanks with exercise and no structure or tanks with neither exercise nor structure. Feed conversion ratio was signif<span style="font-family:Verdana;">icantly improved in the salmon tanks with structure and without exercise<span style="font-family:Verdana;"> com<span style="font-family:;" "=""><span style="font-family:Verdana;">pared to all other treatments. There were no significant differences for indi<span style="font-family:Verdana;">vidual fish weight, total length, specific growth rate, or condition factor<span style="font-family:Verdana;"> among any of the treatments at the end of the experiment. <span style="font-family:Verdana;">These results indicate that the use of both an exercise routine and vertically-suspended structure may be beneficial during the rearing of juvenile landlocked fall Chinook salmon. 展开更多
关键词 Environmental Enrichment SALMONID Oncorhynchus tshawytscha Vertically-Suspended
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Landlocked Fall Chinook Salmon Egg Storage for Delayed Fertilization
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作者 Hunter Eide Michael E. Barnes 《Advances in Bioscience and Biotechnology》 2020年第5期160-165,共6页
Delayed fertilization of salmonid eggs can be a useful option to enhance the efficiency of artificial spawning operations. This experiment examined the fertility of landlocked fall Chinook salmon (Oncorhynchus tshawyt... Delayed fertilization of salmonid eggs can be a useful option to enhance the efficiency of artificial spawning operations. This experiment examined the fertility of landlocked fall Chinook salmon (Oncorhynchus tshawytscha) eggs stored for various times after removal from female broodstock. Fertilization occurred either immediately after spawning or the eggs were stored with oxygen at 11&deg;C and fertilized at 4, 6, 8, 12, or 20 hours post-spawn. Survival to the eyed stage of egg development was significantly greater in the eggs fertilized immediately or after four, six, or eight hours of storage compared to those eggs stored for 12 or 20 hours prior to fertilization (P = 0.024). Survival to hatch was also significantly different (P = 0.018) and followed the same pattern. This study is the first to document the successful fertilization of landlocked fall Chinook salmon eggs after eight hours of storage. 展开更多
关键词 DELAYED FERTILIZATION CHINOOK Salmon EGG STORAGE Oncorhynchus tshawytscha
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Effect of Activated Milt Residence Time on Landlocked Fall Chinook Salmon Egg Survival
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作者 Jessica Shannon Nathan Huysman +2 位作者 Jill M. Voorhees Eric Krebs Michael E. Barnes 《Open Journal of Applied Sciences》 2020年第4期135-141,共7页
During artificial spawning of salmonids, activated sperm is allowed to remain in contact with eggs for variable durations. This study examined multiple residence times (20, 40, 60, 90, or 120 seconds) for activated sp... During artificial spawning of salmonids, activated sperm is allowed to remain in contact with eggs for variable durations. This study examined multiple residence times (20, 40, 60, 90, or 120 seconds) for activated sperm on landlocked fall Chinook salmon (Oncorhynchus tshawytscha) eggs during spawning. There was no significant difference in egg survival to the eyed-stage of development or to hatch among any of the treatments, with a mean (SE) percent survival to the eyed stage of 63.9 (5.8). These results indicate that only relatively short residence times of activated milt on eggs during landlocked fall Chinook salmon spawning are needed to ensure egg survival, resulting in potentially substantial reductions in production-level spawning times and associated labor costs. 展开更多
关键词 CHINOOK Salmon ONCORHYNCHUS tshawytscha MILT EGGS SPAWNING Sperm
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一株大鳞大麻哈鱼源鲑肾杆菌的检测与鉴定 被引量:1
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作者 徐立蒲 王静波 +5 位作者 张文 王小亮 曹欢 王姝 潘勇 江育林 《中国畜牧兽医》 CAS 北大核心 2015年第6期1592-1596,共5页
北京怀柔某养殖场的大鳞大麻哈鱼发生一种慢性,但累计死亡率很高的流行病,为研究大鳞大麻哈鱼感染病原菌的种类,本试验根据病鱼临床症状观察判断疑似感染鲑肾杆菌。试验对病鱼肾脏组织进行印片染色观察细菌形态特征,抽提病鱼的肝脏、肾... 北京怀柔某养殖场的大鳞大麻哈鱼发生一种慢性,但累计死亡率很高的流行病,为研究大鳞大麻哈鱼感染病原菌的种类,本试验根据病鱼临床症状观察判断疑似感染鲑肾杆菌。试验对病鱼肾脏组织进行印片染色观察细菌形态特征,抽提病鱼的肝脏、肾脏组织DNA,扩增16SrDNA和鲑肾杆菌P57表面蛋白基因片段,测序并运用BLAST进行同源性比对。结果显示,病鱼肾脏组织印片在显微镜下可见大量形态一致的革兰氏阳性短杆菌;采用病鱼肝脏、肾脏组织扩增的16SrDNA基因构建的系统发育树显示与鲑肾杆菌聚为一支;通过PCR扩增出鲑肾杆菌P57表面蛋白基因DNA片段,测序结果与鲑肾杆菌标准株ATCC 33209完全一致,符合率为100%。结果表明病鱼感染鲑肾杆菌并引发细菌性肾病(BKD)。这是中国首次在养殖鱼体内检出鲑肾杆菌。 展开更多
关键词 鲑肾杆菌 细菌性肾病 大鳞大马哈鱼
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