To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic a...To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.展开更多
目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜...目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜形成的影响;提取细菌总RNA,逆转录后进行实时荧光定量PCR检测3种培养基对表皮葡萄球菌粘附基因表达量的影响。结果与LB(0.149±0.047)和M-H(0.323±0.003)培养基相比,TSB(2.954±0.287)培养基能显著促进表皮葡萄球菌生物膜的形成(TSB vs LB,t=16.706,P<0.01;TSB vs M-H,t=15.877,P<0.01);与LB培养基相比,TSB培养基可显著促进表皮葡萄球菌ica A基因的表达(t=9.667,P<0.01)并抑制icaR基因的表达(t=13.283,P<0.01)。结论与LB和M-H肉汤培养基相比,TSB培养基能显著促进表皮葡萄球菌生物膜的形成和粘附基因ica A的表达。展开更多
Based on the study of visitors' individual spatial behaviors, a tourists'spatial behavior simulator (TSBS) to assess the carrying capacity of tourist resorts was developed,TSBS employs GIS (Geographic Informat...Based on the study of visitors' individual spatial behaviors, a tourists'spatial behavior simulator (TSBS) to assess the carrying capacity of tourist resorts was developed,TSBS employs GIS (Geographic Information System) to manage the spatial data,and Multi-Agent systemto simulate the actions of individual visitors. By utilizing TSBS, visitors' travel patterns such aslocation, cost, and state can be analyzed and predicted. Based on this analysis and prediction, themodel of assessing the carrying capacity of resorts is built. Our results show that TSBS will be aneffective tool to accurately assess the carrying capacity of tourist resorts.展开更多
文摘To obtain a new lysine rich protein gene, tomato tsb cDNA was synthesized from nearly matural anther mRNA by RT PCR. Sequence analysis indicated that the isolated fragment was composed of 748 bp, encoding a glutamic acid rich (22 55%) and lysine rich (14 89%) protein which was hydrophilic throughout and contained seven imperfect repeated motifs of sequence V V E K K N/E E. It had 70 22% homology to the sequence of SB 401 cDNA reported by Liu et al. The deduced amino acid sequence shares 69 96% identity with the reported gene. Southern blot analysis indicated that tsb was one copy in the tomato genome tsb expressed only in pollen.
文摘目的探讨胰蛋白胨大豆肉汤(TSB)、LB和M-H 3种肉汤培养基对表皮葡萄球菌生物膜形成的影响。方法通过96孔和6孔板构建细菌生物膜,结晶紫染色对其进行半定量分析和显微镜观察生物膜形态,探讨TSB、LB和M-H肉汤培养基对表皮葡萄球菌生物膜形成的影响;提取细菌总RNA,逆转录后进行实时荧光定量PCR检测3种培养基对表皮葡萄球菌粘附基因表达量的影响。结果与LB(0.149±0.047)和M-H(0.323±0.003)培养基相比,TSB(2.954±0.287)培养基能显著促进表皮葡萄球菌生物膜的形成(TSB vs LB,t=16.706,P<0.01;TSB vs M-H,t=15.877,P<0.01);与LB培养基相比,TSB培养基可显著促进表皮葡萄球菌ica A基因的表达(t=9.667,P<0.01)并抑制icaR基因的表达(t=13.283,P<0.01)。结论与LB和M-H肉汤培养基相比,TSB培养基能显著促进表皮葡萄球菌生物膜的形成和粘附基因ica A的表达。
文摘Based on the study of visitors' individual spatial behaviors, a tourists'spatial behavior simulator (TSBS) to assess the carrying capacity of tourist resorts was developed,TSBS employs GIS (Geographic Information System) to manage the spatial data,and Multi-Agent systemto simulate the actions of individual visitors. By utilizing TSBS, visitors' travel patterns such aslocation, cost, and state can be analyzed and predicted. Based on this analysis and prediction, themodel of assessing the carrying capacity of resorts is built. Our results show that TSBS will be aneffective tool to accurately assess the carrying capacity of tourist resorts.
